ATE244759T1 - Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation - Google Patents

Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation

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Publication number
ATE244759T1
ATE244759T1 AT96101903T AT96101903T ATE244759T1 AT E244759 T1 ATE244759 T1 AT E244759T1 AT 96101903 T AT96101903 T AT 96101903T AT 96101903 T AT96101903 T AT 96101903T AT E244759 T1 ATE244759 T1 AT E244759T1
Authority
AT
Austria
Prior art keywords
nucleic acid
compositions
acid amplification
reverse transcriptase
rna polymerase
Prior art date
Application number
AT96101903T
Other languages
English (en)
Inventor
Nancy Lau Liu Shen
Daniel Louis Kacian
James Garfield Putnam
William Michael Davis
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ATE244759T1 publication Critical patent/ATE244759T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1276RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6846Common amplification features
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/81Packaged device or kit
AT96101903T 1995-02-10 1996-02-09 Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation ATE244759T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/387,011 US5556771A (en) 1995-02-10 1995-02-10 Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification

Publications (1)

Publication Number Publication Date
ATE244759T1 true ATE244759T1 (de) 2003-07-15

Family

ID=23528056

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96101903T ATE244759T1 (de) 1995-02-10 1996-02-09 Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation

Country Status (10)

Country Link
US (3) US5556771A (de)
EP (1) EP0726310B1 (de)
JP (1) JP3282819B2 (de)
KR (1) KR19980702110A (de)
AT (1) ATE244759T1 (de)
AU (1) AU699590B2 (de)
CA (1) CA2210584A1 (de)
DE (1) DE69628959T2 (de)
ES (1) ES2202387T3 (de)
WO (1) WO1996024664A1 (de)

Families Citing this family (135)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08131171A (ja) * 1994-11-11 1996-05-28 Asahi Chem Ind Co Ltd 逆転写酵素の安定化組成物
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US6551794B1 (en) * 1995-11-09 2003-04-22 E. R. Squibb & Sons, Inc. Stable biotinylated biomolecule composition
US5876992A (en) * 1996-07-03 1999-03-02 Molecular Biology Resources, Inc. Method and formulation for stabilization of enzymes
DE59611424D1 (de) * 1996-07-16 2007-05-03 Qiagen North American Holdings Verfahren zur herstellung komplexer multienzymatischer lagerstabiler reaktionsgemische und deren verwendung
US6458556B1 (en) * 1996-07-25 2002-10-01 The Institute Of Physical & Chemical Research Method for enhancing enzyme activity at elevated temperature
US6013488A (en) 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
US5861251A (en) * 1996-10-15 1999-01-19 Bioneer Corporation Lyophilized reagent for polymerase chain reaction
EP0868916A3 (de) * 1997-03-04 2004-09-15 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Reduktionshemmungsmittel für Aktiv-Sauerstoff entfernende Aktivität
CN1259957B (zh) 1997-04-22 2012-08-22 茵维特罗根公司 产生由多个亚单位组成的aslv逆转录酶的方法
US6558901B1 (en) 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
US5989499A (en) * 1997-05-02 1999-11-23 Biomerieux, Inc. Dual chamber disposable reaction vessel for amplification reactions
US6429007B1 (en) 1997-05-02 2002-08-06 BIOMéRIEUX, INC. Nucleic acid amplification reaction station for disposable test devices
US6410275B1 (en) 1997-05-02 2002-06-25 Biomerieux, Inc. Disposable test devices for performing nucleic acid amplification reactions
IL124275A (en) 1997-05-02 2002-03-10 Bio Merieux Vitek Inc A method to produce sequences of nucleic acids
GB9717972D0 (en) * 1997-08-22 1997-10-29 Amersham Int Ltd Labelling composition and method
WO1999013113A1 (en) * 1997-09-12 1999-03-18 The Public Health Research Institute Of The City Of New York, Inc. Non-competitive co-amplification methods
US6844158B1 (en) 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
US20040002079A1 (en) * 1998-02-11 2004-01-01 Gunn Robert B. Sodium-phosphate cotransporter in lithium therapy for the treatment of mental illness
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
US6451572B1 (en) * 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
WO2000009086A2 (en) * 1998-08-14 2000-02-24 Valentis, Inc. Protected one-vial formulation for nucleic acid molecules, methods of making the same by in-line mixing, and related products and methods
DE19840531C2 (de) * 1998-08-28 2003-05-15 Roboscreen Ges Fuer Molekulare Mit Nukleinsäuren beschichtete Reaktionsräume, Verfahren zu ihrer Herstellung und ihre Verwendung
US6153412A (en) * 1998-12-07 2000-11-28 Bioneer Corporation Lyophilized reagent for polymerase chain reaction
DK1165806T3 (da) * 1999-03-31 2005-12-05 Cornell Res Foundation Inc Phosphataser med forbedret phytaseaktivitet
US6271004B1 (en) * 1999-06-25 2001-08-07 Display Systems Biotech A/S Method for improved reverse transcription at high temperatures
US6379930B1 (en) * 1999-07-30 2002-04-30 Applied Gene Technologies, Inc. Stabilization of nucleic acid amplification cocktails
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
JP2004511211A (ja) 2000-05-26 2004-04-15 インヴィトロジェン コーポレーション 耐熱性逆転写酵素およびその使用
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
JP2004513617A (ja) * 2000-06-26 2004-05-13 ニューゲン テクノロジーズ, インコーポレイテッド 転写に基づく核酸増幅のための方法および組成物
US6653062B1 (en) 2000-07-26 2003-11-25 Wisconsin Alumni Research Foundation Preservation and storage medium for biological materials
FR2812306B1 (fr) * 2000-07-28 2005-01-14 Gabriel Festoc Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles
US6455256B1 (en) * 2000-08-04 2002-09-24 E. & J. Gallo Winery Method of amplifying nucleic acids of microorganisms present in fruit juice
ATE316581T1 (de) 2000-09-05 2006-02-15 Zeltz Patrick Dr Verfahren zur spezifischen bestimmung von dna- sequenzen mittels paralleler amplifikation
CA2430329A1 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
BR0205268A (pt) 2001-03-09 2004-11-30 Nugen Technologies Inc Processos e composições para a mplificação de sequências de rna
ES2180416B1 (es) * 2001-03-12 2004-06-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen.
BRPI0213813B1 (pt) 2001-10-31 2018-11-21 Cornell Res Foundation Inc método para aprimorar o valor nutricional de uma ração consumida por um animal monogástrico e ração
EP1452593B1 (de) * 2001-11-14 2009-04-08 Toyo Boseki Kabushiki Kaisha Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon
EP2455488B1 (de) * 2002-04-12 2017-06-07 New England Biolabs, Inc. Verfahren und zusammensetzung zur dna-manipulation
ES2579803T3 (es) * 2002-09-03 2016-08-16 Quanta Biosciences, Inc. Composiciones y métodos mejorados para la síntesis de ADNc
WO2004024885A2 (en) * 2002-09-13 2004-03-25 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
EP2325303B1 (de) 2002-09-13 2013-11-06 Life Technologies Corporation Wärmestabile reverse Transkriptasen und Verwendungen davon
EP1546386A4 (de) * 2002-09-17 2006-05-10 Pharmacia Corp Erhaltung von rna und reverser transkriptase während der automatisierten handhabung von flüssigkeiten
US20040053319A1 (en) * 2002-09-17 2004-03-18 Mcwilliams Diana R. Isolation of genetic molecules from a complex biological constrauct for use in genetic expression analysis
US7407747B2 (en) * 2002-10-15 2008-08-05 Applera Corporation Method for drying dye-terminator sequencing reagents
DE10252239B4 (de) * 2002-11-07 2006-08-17 Micronas Holding Gmbh Verfahren zum Drucken von Biomolekülen
JP2006505283A (ja) * 2002-11-08 2006-02-16 フアルマシア・コーポレーシヨン 高速処理自動化核酸単離及び定量法
US20040101859A1 (en) * 2002-11-25 2004-05-27 Cepheid Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
ES2214144B1 (es) * 2003-02-26 2005-09-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Composicion estabilizada para ensayos fluorimetricos, colorimetricos o quimio-luminiscentes, kits que la contienen y procedimiento para su obtencion.
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
WO2004092418A2 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
JP4836795B2 (ja) * 2003-05-19 2011-12-14 ブランデイズ ユニバーシティー 核酸プロセシング方法、キット、及び装置
FR2855177B1 (fr) * 2003-05-23 2005-07-08 Raymond Robert Composition contenant des acides nucleiques lyophilises en presence de collagene et de disaccharides
US20050048460A1 (en) * 2003-05-29 2005-03-03 The Regents Of The University Of California Preservative and method for preserving cells
US20090011488A1 (en) * 2003-08-18 2009-01-08 Rosetta Inpharmatics, Llc Methods for storing compositions useful for synthesizing nucleic acid molecules
JPWO2005030958A1 (ja) * 2003-09-30 2007-11-15 独立行政法人理化学研究所 酵素が固定されている支持体、印刷物、試薬キット、該支持体の製造法、酵素の保存法及び酵素の再生法
EP1737573A2 (de) 2004-04-08 2007-01-03 Biomatrica, Inc. Integration von probenlagerung und probenverwaltung für biowissenschaften
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
FI20040768A0 (fi) * 2004-06-04 2004-06-04 Teemu Korpimaeki Menetelmä määritysreagenssien stabiloimiseksi, stabilisoituja määritysreagensseja sisältävä reagenssisäiliö ja sen käyttö
EP1975242B1 (de) 2004-08-27 2011-03-02 Gen-Probe Incorporated Einfach-Primernukleinsäuren-Erweiterungsverfahren
US20060068399A1 (en) * 2004-09-24 2006-03-30 Cepheid Multiple bead reagent system for protein based assays with optimized matrices
US20060068398A1 (en) * 2004-09-24 2006-03-30 Cepheid Universal and target specific reagent beads for nucleic acid amplification
JP4699008B2 (ja) * 2004-11-02 2011-06-08 旭化成株式会社 酵素反応試薬
FR2878254B1 (fr) * 2004-11-22 2010-08-20 Bio Rad Pasteur Composition pour l'amplification d'acides nucleiques
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
WO2006119280A2 (en) * 2005-05-03 2006-11-09 Handylab, Inc. Lyophilized pellets
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US8168206B1 (en) 2005-10-06 2012-05-01 Allergan, Inc. Animal protein-free pharmaceutical compositions
US8137677B2 (en) * 2005-10-06 2012-03-20 Allergan, Inc. Non-protein stabilized clostridial toxin pharmaceutical compositions
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
DK1945821T3 (da) 2006-06-06 2011-03-07 Gen Probe Inc Mærkede oligonukleotider og anvendelse deraf i fremgangsmåder til amplifikation af nukleinsyrer
US8187557B2 (en) * 2006-07-13 2012-05-29 Cepheid Reagent reservoir system for analytical instruments
US8540984B2 (en) * 2006-08-03 2013-09-24 Cornell Research Foundation, Inc. Phytases with improved thermal stability
DE102006056790B3 (de) * 2006-12-01 2008-01-17 IfP Privates Institut für Produktqualität GmbH Laboreinmalartikel für Analyse und Diagnosik
EP2108048A1 (de) * 2007-01-17 2009-10-14 Meridian Bioscience, Inc. Stabile reagenzien und kits für loop-vermittelte isotherme verstärkung
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
US7738094B2 (en) 2007-01-26 2010-06-15 Becton, Dickinson And Company Method, system, and compositions for cell counting and analysis
KR100984483B1 (ko) * 2007-03-19 2010-10-01 (주)바이오니아 순환적 역전사 방법
BRPI0810516A2 (pt) * 2007-04-24 2014-10-07 Biomatrica Inc Armazenamento de amostra para ciência da vida
KR20100017126A (ko) * 2007-04-25 2010-02-16 쓰리엠 이노베이티브 프로퍼티즈 컴파니 화학적 요소 및 처리 장치 조립체
ES2453379T3 (es) * 2007-04-25 2014-04-07 3M Innovative Properties Company Reactivos soportados, métodos y dispositivos
GB0711683D0 (en) * 2007-06-16 2007-07-25 Enigma Diagnostics Ltd Compositions
CN101970111B (zh) 2007-06-21 2013-09-11 简·探针公司 用于执行处理的仪器和容器
US20110014676A1 (en) * 2007-06-29 2011-01-20 Battelle Memorial Institute Protein stabilization
US7816135B2 (en) 2007-07-05 2010-10-19 Becton, Dickinson And Company Method of analyzing lymphocytes
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
KR101098764B1 (ko) * 2007-10-29 2011-12-26 (주)바이오니아 안정화된 핫스타트 pcr용 건조 조성물
US20100209927A1 (en) * 2007-11-06 2010-08-19 Menon Vinod P Processing device tablet
WO2009086218A2 (en) 2007-12-21 2009-07-09 Gen-Probe Incorporated Detection of antibiotic-resistant microorganisms
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
KR100978215B1 (ko) * 2008-02-28 2010-08-26 주식회사 인트론바이오테크놀로지 건조형태의 pcr 반응용 조성물에 기반한 pcr의 최적증폭 조건 탐색 및 설정에 활용될 수 있는 키트 및 그것의제조방법
WO2009117698A2 (en) * 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
EP2806037B1 (de) 2008-05-13 2016-09-21 Gen-Probe Incorporated Inaktivierbare Target-Capture-Oligomere zur Verwendung bei der selektiven Hybridisierung und beim Fangen von Zielnukleinsäuresequenzen
GB0814570D0 (en) 2008-08-08 2008-09-17 Diagnostics For The Real World Isolation of nucleic acid
EP2391452B1 (de) 2009-01-30 2015-06-17 Gen-Probe Incorporated Systeme und verfahren zur erfassung eines signals und beaufschlagung des signalübertragungselements mit wärmeenergie
CA2761675A1 (en) * 2009-05-11 2010-11-18 Biomatrica, Inc. Compositions and methods for biological sample storage
IT1394539B1 (it) * 2009-05-19 2012-07-05 Sentinel Ch S P A Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso
US20130157265A1 (en) 2009-10-22 2013-06-20 Jesus Mingorance Cruz Composition, method and kit for detecting bacteria by means of sequencing
EP2566985A4 (de) * 2010-05-06 2014-08-06 Ibis Biosciences Inc Integrierte probenpräparationssysteme und stabilisierte enzymmischungen
DE102010038330A1 (de) * 2010-07-23 2012-03-01 Aj Innuscreen Gmbh Verfahren, Vorrichtung und Testkit für Molekularbiologische Reaktionen
EP2598661B1 (de) 2010-07-26 2017-09-27 Biomatrica, INC. Zusammensetzungen zur stabilisierung von dna, rna und proteinen in speichel- und anderen biologischen proben während des transports und der lagerung bei umgebungstemperaturen
US9376709B2 (en) 2010-07-26 2016-06-28 Biomatrica, Inc. Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures
CN103140145B (zh) 2010-08-13 2014-08-20 高级生物营养公司 用于生物材料的干的贮存稳定用组合物
WO2012054727A1 (en) 2010-10-22 2012-04-26 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
US20130023010A1 (en) * 2011-07-21 2013-01-24 Genereach Biotechnology Corp. Method for reverse transcription polymerase chain reaction
US8192659B1 (en) 2011-09-12 2012-06-05 On Demand Therapeutics, Inc. Methods of making microtablets for drug delivery
WO2013071168A1 (en) 2011-11-11 2013-05-16 Gen-Probe Gti Diagnostics, Inc., C/O Gen-Probe Incorporated Polypeptide substrate for the detection of von willebrand factor cleaving protease adamts13
EP2824172B1 (de) * 2012-03-08 2017-09-06 Sony Corporation Verfahren zur herstellung eines mikrochips zur verwendung bei einer nucleinsäureamplifikationsreaktion
KR101870311B1 (ko) * 2012-03-09 2018-06-25 (주)바이오니아 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물
RU2535995C2 (ru) * 2012-06-01 2014-12-20 Общество с ограниченной ответственностью "ИнтерЛабСервис" Сухая смесь для приготовления реакционной смеси для амплификации нуклеиновой кислоты и способ ее получения
EP2904120B1 (de) 2012-10-04 2018-01-10 The Board of Trustees of The Leland Stanford Junior University Verfahren und reagenzien für den nachweis, die quantifizierung und serotypisierung von dengueviren
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
KR20140110138A (ko) * 2013-03-04 2014-09-17 우송대학교 산학협력단 교차오염 억제용 udg를 함유한 중합효소연쇄반응액의 동결건조물
JP6413228B2 (ja) * 2013-11-13 2018-10-31 東ソー株式会社 長期保存可能な核酸増幅試薬
EP3097190A2 (de) 2014-01-22 2016-11-30 Life Technologies Corporation Neuartige reverse transkriptasen zur verwendung bei hochtemperatur-nukleinsäuresynthese
CN107075566B (zh) * 2014-01-27 2021-06-15 阿谢尔德克斯有限责任公司 用于制备核酸的等温方法及相关组合物
JP6661554B2 (ja) 2014-06-10 2020-03-11 バイオマトリカ,インク. 周囲温度における血小板の安定化
JP6403809B2 (ja) * 2014-06-18 2018-10-10 ルミネックス コーポレーション 安定化させた凍結乾燥物質を作製するための方法
JP6890539B2 (ja) * 2015-05-08 2021-06-18 株式会社カネカ 乾燥形態の酵素反応試薬およびその調製法
SG11201804776SA (en) 2015-12-08 2018-07-30 Biomatrica Inc Reduction of erythrocyte sedimentation rate
KR20180103945A (ko) * 2016-02-05 2018-09-19 젠-프로브 인코포레이티드 건조된 증폭 조성물
FI126979B (en) * 2016-02-29 2017-09-15 Faron Pharmaceuticals Oy Lyophilized pharmaceutical formulation and use thereof
ES2755815T3 (es) 2016-09-13 2020-04-23 Allergan Inc Composiciones de toxina de Clostridium no proteicas estabilizadas
CA3037190A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free dna
ES2903357T3 (es) 2016-09-15 2022-04-01 Archerdx Llc Métodos de preparación de muestras de ácido nucleico
CN110506126A (zh) 2017-05-19 2019-11-26 简·探针公司 含有瓣状核酸内切酶的干燥组合物
US11268084B2 (en) 2017-08-08 2022-03-08 Thermo Fisher Scientific Baltics, UAB Glycerol-free formulations for reverse transcriptases
CN107365754A (zh) * 2017-09-04 2017-11-21 广州和实生物技术有限公司 一种m‑mlv逆转录酶的冻干工艺
US20230265531A1 (en) 2020-07-22 2023-08-24 King Abdullah University Of Science And Technology Compositions and methods for one-step nucleic acid amplification and diagnostic methods based thereon
CN116121349B (zh) * 2022-12-20 2024-04-09 南京诺唯赞生物科技股份有限公司 一种扩增含乙醇的核酸样本的方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS589688A (ja) * 1981-07-06 1983-01-20 Toyobo Co Ltd 安定な酵素組成物
HU189251B (en) * 1982-04-07 1986-06-30 Asahi Kasei Kogyo Kk,Jp Process for stabilizing tumor necrosis factor
WO1987000196A1 (en) * 1985-07-09 1987-01-15 Quadrant Bioresources Limited Protection of proteins and the like
US4806478A (en) * 1984-10-17 1989-02-21 Minnesota Mining And Manufacturing Company Dry enzyme formulations containing D-amino acid oxidase
CA1294215C (en) * 1986-10-27 1992-01-14 Ze'ev Shaked Pharmaceutical compositions of recombinant beta-interferon and formulation processes
GB8715238D0 (en) * 1987-06-29 1987-08-05 Quadrant Bioresources Ltd Food process
GB8801338D0 (en) * 1988-01-21 1988-02-17 Quadrant Bioresources Ltd Preservation of viruses
US5262296A (en) * 1988-03-30 1993-11-16 Toray Industries, Inc. Freeze-dried composition containing enzyme-labeled anti-human interferon-β antibody and enzyme immunoassay kit containing the composition
GB8826429D0 (en) * 1988-11-11 1988-12-14 Univ Leeds Ind Service Ltd Enzyme stabilisation systems
GB8927365D0 (en) * 1989-12-04 1990-01-31 Ici Plc Reagent
IE64738B1 (en) * 1990-03-20 1995-09-06 Akzo Nv Stabilized gonadotropin containing preparations
WO1993000807A1 (en) * 1991-07-03 1993-01-21 Cryolife, Inc. Method for stabilization of biomaterials
US5250429A (en) * 1991-09-20 1993-10-05 Pharmacia P-L Biochemicals Inc. Glassified restriction enzymes
CA2127877A1 (en) * 1992-01-21 1993-07-22 Robert M. Platz Improved process for preparing micronized polypeptide drugs
WO1994016107A1 (en) * 1993-01-12 1994-07-21 Thomas Jefferson University DNA SEQUENCING WITH Bst POLYMERASE
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification

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ES2202387T3 (es) 2004-04-01
AU4916796A (en) 1996-08-27
US5614387A (en) 1997-03-25
EP0726310A1 (de) 1996-08-14
US5556771A (en) 1996-09-17
DE69628959T2 (de) 2004-05-13
WO1996024664A1 (en) 1996-08-15
JP3282819B2 (ja) 2002-05-20
EP0726310B1 (de) 2003-07-09
KR19980702110A (ko) 1998-07-15
DE69628959D1 (de) 2003-08-14
US5834254A (en) 1998-11-10
CA2210584A1 (en) 1996-08-15
JPH10503383A (ja) 1998-03-31

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