ATE244759T1 - Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation - Google Patents

Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation

Info

Publication number
ATE244759T1
ATE244759T1 AT96101903T AT96101903T ATE244759T1 AT E244759 T1 ATE244759 T1 AT E244759T1 AT 96101903 T AT96101903 T AT 96101903T AT 96101903 T AT96101903 T AT 96101903T AT E244759 T1 ATE244759 T1 AT E244759T1
Authority
AT
Austria
Prior art keywords
nucleic acid
compositions
acid amplification
reverse transcriptase
rna polymerase
Prior art date
Application number
AT96101903T
Other languages
English (en)
Inventor
Nancy Lau Liu Shen
Daniel Louis Kacian
James Garfield Putnam
William Michael Davis
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ATE244759T1 publication Critical patent/ATE244759T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
AT96101903T 1995-02-10 1996-02-09 Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation ATE244759T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/387,011 US5556771A (en) 1995-02-10 1995-02-10 Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification

Publications (1)

Publication Number Publication Date
ATE244759T1 true ATE244759T1 (de) 2003-07-15

Family

ID=23528056

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96101903T ATE244759T1 (de) 1995-02-10 1996-02-09 Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation

Country Status (10)

Country Link
US (3) US5556771A (de)
EP (1) EP0726310B1 (de)
JP (1) JP3282819B2 (de)
KR (1) KR19980702110A (de)
AT (1) ATE244759T1 (de)
AU (1) AU699590B2 (de)
CA (1) CA2210584A1 (de)
DE (1) DE69628959T2 (de)
ES (1) ES2202387T3 (de)
WO (1) WO1996024664A1 (de)

Families Citing this family (140)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08131171A (ja) * 1994-11-11 1996-05-28 Asahi Chem Ind Co Ltd 逆転写酵素の安定化組成物
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
US6551794B1 (en) * 1995-11-09 2003-04-22 E. R. Squibb & Sons, Inc. Stable biotinylated biomolecule composition
US5876992A (en) * 1996-07-03 1999-03-02 Molecular Biology Resources, Inc. Method and formulation for stabilization of enzymes
ATE357513T1 (de) * 1996-07-16 2007-04-15 Qiagen North American Holdings Verfahren zur herstellung komplexer multienzymatischer lagerstabiler reaktionsgemische und deren verwendung
US6013488A (en) 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
US6458556B1 (en) * 1996-07-25 2002-10-01 The Institute Of Physical & Chemical Research Method for enhancing enzyme activity at elevated temperature
US5861251A (en) * 1996-10-15 1999-01-19 Bioneer Corporation Lyophilized reagent for polymerase chain reaction
TW466116B (en) * 1997-03-04 2001-12-01 Hayashibara Biochem Lab Reduction inhibitory agent for active-oxygen eliminating activity, method for inhibiting the reduction of said activity, and composition containing said agent
EP1005481B1 (de) 1997-04-22 2009-10-14 Life Technologies Corporation Verfahren zur herstellung von aslv reverser transkriptase zusammengestellt aus mehreren untereinheiten
US6558901B1 (en) * 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
US6429007B1 (en) 1997-05-02 2002-08-06 BIOMéRIEUX, INC. Nucleic acid amplification reaction station for disposable test devices
US6410275B1 (en) 1997-05-02 2002-06-25 Biomerieux, Inc. Disposable test devices for performing nucleic acid amplification reactions
US5989499A (en) * 1997-05-02 1999-11-23 Biomerieux, Inc. Dual chamber disposable reaction vessel for amplification reactions
IL124275A (en) * 1997-05-02 2002-03-10 Bio Merieux Vitek Inc A method to produce sequences of nucleic acids
GB9717972D0 (en) * 1997-08-22 1997-10-29 Amersham Int Ltd Labelling composition and method
EP1012344A4 (de) 1997-09-12 2004-08-11 Public Health Res Instituteof Nicht-kompetitive co-amplifizierungsmethoden
US6844158B1 (en) 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
US20040002079A1 (en) * 1998-02-11 2004-01-01 Gunn Robert B. Sodium-phosphate cotransporter in lithium therapy for the treatment of mental illness
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
US6451572B1 (en) * 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
JP4538150B2 (ja) * 1998-08-14 2010-09-08 ヴァレンティス，インコーポレイテッド 核酸分子の保護された単一バイアル調製物、インライン混合によってそれを形成する方法、及び関連する生成物及び方法
DE19840531C2 (de) * 1998-08-28 2003-05-15 Roboscreen Ges Fuer Molekulare Mit Nukleinsäuren beschichtete Reaktionsräume, Verfahren zu ihrer Herstellung und ihre Verwendung
US6153412A (en) * 1998-12-07 2000-11-28 Bioneer Corporation Lyophilized reagent for polymerase chain reaction
EP1165806B1 (de) * 1999-03-31 2005-08-03 Cornell Research Foundation, Inc. Phospahatasen mit verbesserter phytase-aktivität
US6271004B1 (en) * 1999-06-25 2001-08-07 Display Systems Biotech A/S Method for improved reverse transcription at high temperatures
US6379930B1 (en) * 1999-07-30 2002-04-30 Applied Gene Technologies, Inc. Stabilization of nucleic acid amplification cocktails
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
CN1430670A (zh) 2000-05-26 2003-07-16 茵维特罗根公司 热稳定逆转录酶及其用途
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
ATE455186T1 (de) * 2000-06-26 2010-01-15 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
US6653062B1 (en) * 2000-07-26 2003-11-25 Wisconsin Alumni Research Foundation Preservation and storage medium for biological materials
FR2812306B1 (fr) * 2000-07-28 2005-01-14 Gabriel Festoc Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles
US6455256B1 (en) * 2000-08-04 2002-09-24 E. & J. Gallo Winery Method of amplifying nucleic acids of microorganisms present in fruit juice
EP1186669B1 (de) 2000-09-05 2006-01-25 Zeltz, Patrick, Dr. Verfahren zur spezifischen Bestimmung von DNA-Sequenzen mittels paralleler Amplifikation
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
KR20030082535A (ko) 2001-03-09 2003-10-22 뉴젠 테크놀로지스 인코포레이티드 Ｒｎａ 서열의 증폭을 위한 방법 및 조성물
ES2180416B1 (es) * 2001-03-12 2004-06-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen.
PT2335501T (pt) * 2001-10-31 2016-10-28 Huvepharma Ad Alimento animal contendo fitase e método
DE60231901D1 (de) 2001-11-14 2009-05-20 Toyo Boseki Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon
US7435572B2 (en) * 2002-04-12 2008-10-14 New England Biolabs, Inc. Methods and compositions for DNA manipulation
ES2579803T3 (es) * 2002-09-03 2016-08-16 Quanta Biosciences, Inc. Composiciones y métodos mejorados para la síntesis de ADNc
EP2325303B1 (de) 2002-09-13 2013-11-06 Life Technologies Corporation Wärmestabile reverse Transkriptasen und Verwendungen davon
DE60335497D1 (de) * 2002-09-13 2011-02-03 Cornell Res Foundation Inc Ergillus-phytasen
US20040053319A1 (en) * 2002-09-17 2004-03-18 Mcwilliams Diana R. Isolation of genetic molecules from a complex biological constrauct for use in genetic expression analysis
JP2005538734A (ja) * 2002-09-17 2005-12-22 フアルマシア・コーポレーシヨン 自動液体処理中のｒｎａ及び逆転写酵素の保存
US7407747B2 (en) 2002-10-15 2008-08-05 Applera Corporation Method for drying dye-terminator sequencing reagents
DE10252239B4 (de) * 2002-11-07 2006-08-17 Micronas Holding Gmbh Verfahren zum Drucken von Biomolekülen
JP2006505283A (ja) * 2002-11-08 2006-02-16 フアルマシア・コーポレーシヨン 高速処理自動化核酸単離及び定量法
US20040101859A1 (en) * 2002-11-25 2004-05-27 Cepheid Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
ES2214144B1 (es) * 2003-02-26 2005-09-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Composicion estabilizada para ensayos fluorimetricos, colorimetricos o quimio-luminiscentes, kits que la contienen y procedimiento para su obtencion.
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
ES2355899T3 (es) * 2003-05-19 2011-04-01 Brandeis University Procedimientos, kits y dispositivos de tratamiento de ácidos nucleicos.
FR2855177B1 (fr) * 2003-05-23 2005-07-08 Raymond Robert Composition contenant des acides nucleiques lyophilises en presence de collagene et de disaccharides
US20050048460A1 (en) * 2003-05-29 2005-03-03 The Regents Of The University Of California Preservative and method for preserving cells
US20090011488A1 (en) * 2003-08-18 2009-01-08 Rosetta Inpharmatics, Llc Methods for storing compositions useful for synthesizing nucleic acid molecules
US20070117094A1 (en) * 2003-09-30 2007-05-24 Yoshihide Hayashizaki Support, printed material and reagent kit having enzyme fixed thereon, method for preparing the support, method of storing enzyme and method for restoration enzymes
AU2005245338B2 (en) * 2004-04-08 2011-11-17 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
FI20040768A0 (fi) 2004-06-04 2004-06-04 Teemu Korpimaeki Menetelmä määritysreagenssien stabiloimiseksi, stabilisoituja määritysreagensseja sisältävä reagenssisäiliö ja sen käyttö
EP2071031B1 (de) 2004-08-27 2013-10-09 Gen-Probe Incorporated Einfach-Primernukleinsäuren-Erweiterungsverfahren
US20060068398A1 (en) * 2004-09-24 2006-03-30 Cepheid Universal and target specific reagent beads for nucleic acid amplification
US20060068399A1 (en) * 2004-09-24 2006-03-30 Cepheid Multiple bead reagent system for protein based assays with optimized matrices
JP4699008B2 (ja) * 2004-11-02 2011-06-08 旭化成株式会社 酵素反応試薬
FR2878254B1 (fr) * 2004-11-22 2010-08-20 Bio Rad Pasteur Composition pour l'amplification d'acides nucleiques
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
WO2006119280A2 (en) * 2005-05-03 2006-11-09 Handylab, Inc. Lyophilized pellets
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US8137677B2 (en) * 2005-10-06 2012-03-20 Allergan, Inc. Non-protein stabilized clostridial toxin pharmaceutical compositions
US8168206B1 (en) 2005-10-06 2012-05-01 Allergan, Inc. Animal protein-free pharmaceutical compositions
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
WO2007146154A1 (en) 2006-06-06 2007-12-21 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US8187557B2 (en) 2006-07-13 2012-05-29 Cepheid Reagent reservoir system for analytical instruments
WO2008017066A2 (en) * 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
DE102006056790B3 (de) * 2006-12-01 2008-01-17 IfP Privates Institut für Produktqualität GmbH Laboreinmalartikel für Analyse und Diagnosik
CA2673772A1 (en) * 2007-01-17 2008-07-24 Meridian Bioscience, Inc. Stable reagents and kits useful in loop-mediated isothermal amplification (lamp)
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
US7738094B2 (en) 2007-01-26 2010-06-15 Becton, Dickinson And Company Method, system, and compositions for cell counting and analysis
KR100984483B1 (ko) * 2007-03-19 2010-10-01 (주)바이오니아 순환적 역전사 방법
WO2009009210A2 (en) * 2007-04-24 2009-01-15 Biomatrica, Inc. Sample storage for life science
KR20100017126A (ko) * 2007-04-25 2010-02-16 쓰리엠 이노베이티브 프로퍼티즈 컴파니 화학적 요소 및 처리 장치 조립체
US8835157B2 (en) * 2007-04-25 2014-09-16 3M Innovative Properties Company Supported reagents, methods, and devices
GB0711683D0 (en) * 2007-06-16 2007-07-25 Enigma Diagnostics Ltd Compositions
JP5282088B2 (ja) 2007-06-21 2013-09-04 ジェン−プロウブ インコーポレイテッド 処理実行に使用する機器および容器
WO2009006301A2 (en) * 2007-06-29 2009-01-08 Battelle Memorial Institute Protein stabilization
US7816135B2 (en) 2007-07-05 2010-10-19 Becton, Dickinson And Company Method of analyzing lymphocytes
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
KR101098764B1 (ko) * 2007-10-29 2011-12-26 (주)바이오니아 안정화된 핫스타트 ｐｃｒ용 건조 조성물
US20100209927A1 (en) * 2007-11-06 2010-08-19 Menon Vinod P Processing device tablet
US8911946B2 (en) 2007-12-21 2014-12-16 Gen-Probe Incorporated Detection of antibiotic-resistant microorganisms
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
KR100978215B1 (ko) * 2008-02-28 2010-08-26 주식회사 인트론바이오테크놀로지 건조형태의 ｐｃｒ 반응용 조성물에 기반한 ｐｃｒ의 최적증폭 조건 탐색 및 설정에 활용될 수 있는 키트 및 그것의제조방법
GB2470672B (en) * 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
WO2009140374A2 (en) 2008-05-13 2009-11-19 Gen-Probe Incorporated Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
GB0814570D0 (en) 2008-08-08 2008-09-17 Diagnostics For The Real World Isolation of nucleic acid
CA2750900C (en) 2009-01-30 2017-03-28 Gen-Probe Incorporated Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
EP2430195B1 (de) * 2009-05-11 2019-01-23 Biomatrica, INC. Zusammensetzungen und verfahren zur lagerung biologischer proben
IT1394539B1 (it) * 2009-05-19 2012-07-05 Sentinel Ch S P A Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso
WO2011048227A1 (es) 2009-10-22 2011-04-28 Biotools Biotechnological & Medical Laboratories, S.A. Composición, método y kit para la detección de bacterias mediante secuenciación
SG10201503540QA (en) * 2010-05-06 2015-06-29 Ibis Biosciences Inc Integrated sample preparation systems and stabilized enzyme mixtures
DE102010038330A1 (de) * 2010-07-23 2012-03-01 Aj Innuscreen Gmbh Verfahren, Vorrichtung und Testkit für Molekularbiologische Reaktionen
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
WO2012018639A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
MX350047B (es) 2010-08-13 2017-08-24 Advanced Bionutrition Corp Composicion de estabilizacion de almacenamiento en seco para materiales biologicos.
US9458484B2 (en) 2010-10-22 2016-10-04 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
WO2013010333A1 (zh) * 2011-07-21 2013-01-24 瑞基海洋生物科技股份有限公司 反转录聚合酶链式反应的方法
US8192659B1 (en) 2011-09-12 2012-06-05 On Demand Therapeutics, Inc. Methods of making microtablets for drug delivery
JP6419576B2 (ja) 2011-11-11 2018-11-07 イムコア ジーティーアイ ダイアグノスティクス インコーポレイテッド プロテアーゼａｄａｍｔｓ１３を切断するフォン・ヴィレブランド因子の検出用ポリペプチド基体
RU2014135538A (ru) * 2012-03-08 2016-03-20 Сони Корпорейшн Способ изготовления микрочипа для реакции амплификации нуклеиновых кислот
KR101870311B1 (ko) 2012-03-09 2018-06-25 (주)바이오니아 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물
RU2535995C2 (ru) * 2012-06-01 2014-12-20 Общество с ограниченной ответственностью "ИнтерЛабСервис" Сухая смесь для приготовления реакционной смеси для амплификации нуклеиновой кислоты и способ ее получения
MY167603A (en) 2012-10-04 2018-09-20 The Board Of Trustees Of Leland Stanford Junior Univ Method and reagents for detection, quantitation, and serotyping of dengue viruses
EP3249054A1 (de) * 2012-12-20 2017-11-29 Biomatrica, INC. Formulierungen und verfahren zur stabilisierung von pcr-reagenzien
KR20140110138A (ko) * 2013-03-04 2014-09-17 우송대학교 산학협력단 교차오염 억제용 ｕｄｇ를 함유한 중합효소연쇄반응액의 동결건조물
JP6413228B2 (ja) * 2013-11-13 2018-10-31 東ソー株式会社 長期保存可能な核酸増幅試薬
US9663770B2 (en) 2014-01-22 2017-05-30 Life Technologies Corporation Reverse transcriptases for use in high temperature nucleic acid synthesis
US20150337364A1 (en) * 2014-01-27 2015-11-26 ArcherDX, Inc. Isothermal Methods and Related Compositions for Preparing Nucleic Acids
WO2015191632A1 (en) 2014-06-10 2015-12-17 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
KR20170019458A (ko) 2014-06-18 2017-02-21 루미넥스 코포레이션 안정화되고 동결건조된 물질을 생성하는 방법
WO2016181897A1 (ja) * 2015-05-08 2016-11-17 株式会社カネカ 乾燥形態の酵素反応試薬およびその調製法
KR102589056B1 (ko) 2015-12-08 2023-10-12 바이오매트리카 인코포레이티드 적혈구 침강 속도의 감소
EP4286529A3 (de) * 2016-02-05 2024-03-27 Gen-Probe Incorporated Getrocknete verstärkungszusammensetzungen
FI126979B (en) * 2016-02-29 2017-09-15 Faron Pharmaceuticals Oy Lyophilized pharmaceutical formulation and its use
IL265109B2 (en) 2016-09-13 2024-05-01 Allergan Inc Stabilized non-protein clostridial toxin compositions
JP6997773B2 (ja) 2016-09-15 2022-01-18 アーチャーディーエックス， エルエルシー 無細胞ｄｎａの分析用の核酸サンプル調製の方法
CA3037185A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
EP4219771A3 (de) * 2017-03-24 2023-08-16 Gen-Probe Incorporated Zusammensetzungen und verfahren für den multiplexnachweis viraler krankheitserreger in proben
DE112018002597B4 (de) 2017-05-19 2024-05-02 Gen-Probe Incorporated Getrocknete zusammensetzungen, die flap-endonuklease enthalten
EP3665278A1 (de) 2017-08-08 2020-06-17 Thermo Fisher Scientific Baltics UAB Glycerinfreie formulierungen für reverse-transkriptasen
CN107365754A (zh) * 2017-09-04 2017-11-21 广州和实生物技术有限公司 一种m‑mlv逆转录酶的冻干工艺
US20220195494A1 (en) * 2019-04-25 2022-06-23 Northwestern University Stabilization and preservation of in vitro transcription reactions
WO2022018685A1 (en) 2020-07-22 2022-01-27 King Abdullah University Of Science And Technology Compositions and methods for one-step nucleic acid amplification and diagnostic methods based thereon
CN116121349B (zh) * 2022-12-20 2024-04-09 南京诺唯赞生物科技股份有限公司 一种扩增含乙醇的核酸样本的方法
GB2630305A (en) 2023-05-22 2024-11-27 Thermo Fisher Scientific Baltics Uab Reverse transcriptase with improved properties
WO2024240872A1 (en) 2023-05-23 2024-11-28 Biocartis Nv Cartridges comprising tablets
WO2026006318A1 (en) 2024-06-24 2026-01-02 Amplifidx, Inc. Point-of-care bioassay systems and methods of making and using the same

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS589688A (ja) * 1981-07-06 1983-01-20 Toyobo Co Ltd 安定な酵素組成物
US4447355A (en) * 1982-04-07 1984-05-08 Asahi Kasei Kogyo Kabushiki Kaisha Method for stabilizing a tumor necrosis factor and a stable aqueous solution or powder containing the same
US4806478A (en) * 1984-10-17 1989-02-21 Minnesota Mining And Manufacturing Company Dry enzyme formulations containing D-amino acid oxidase
ATE68524T1 (de) * 1985-07-09 1991-11-15 Quadrant Bioresources Ltd Beschuetzung von proteinen und aehnlichem.
CA1294215C (en) * 1986-10-27 1992-01-14 Ze'ev Shaked Pharmaceutical compositions of recombinant beta-interferon and formulation processes
GB8715238D0 (en) * 1987-06-29 1987-08-05 Quadrant Bioresources Ltd Food process
GB8801338D0 (en) * 1988-01-21 1988-02-17 Quadrant Bioresources Ltd Preservation of viruses
EP0365685B1 (de) * 1988-03-30 1995-01-18 Toray Industries, Inc. Gefriergetrocknete Zusammensetzung, die ein mit Meerrettichperoxydase markiertes Fab'-Fragment eines gegen menschliches Beta-Interferon gerichteten Antikörpers und Trehalose enthält; EIA Kit, der diese Zusammensetzung enthält
GB8826429D0 (en) * 1988-11-11 1988-12-14 Univ Leeds Ind Service Ltd Enzyme stabilisation systems
GB8927365D0 (en) * 1989-12-04 1990-01-31 Ici Plc Reagent
IE64738B1 (en) * 1990-03-20 1995-09-06 Akzo Nv Stabilized gonadotropin containing preparations
WO1993000807A1 (en) * 1991-07-03 1993-01-21 Cryolife, Inc. Method for stabilization of biomaterials
US5250429A (en) * 1991-09-20 1993-10-05 Pharmacia P-L Biochemicals Inc. Glassified restriction enzymes
JPH07503154A (ja) * 1992-01-21 1995-04-06 エス・アール・アイ・インターナシヨナル 微細化ポリペプチド薬剤の改良調製方法
WO1994016107A1 (en) * 1993-01-12 1994-07-21 Thomas Jefferson University DNA SEQUENCING WITH Bst POLYMERASE
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification

Also Published As

Publication number Publication date
WO1996024664A1 (en) 1996-08-15
AU4916796A (en) 1996-08-27
JP3282819B2 (ja) 2002-05-20
EP0726310B1 (de) 2003-07-09
ES2202387T3 (es) 2004-04-01
KR19980702110A (ko) 1998-07-15
JPH10503383A (ja) 1998-03-31
DE69628959D1 (de) 2003-08-14
US5834254A (en) 1998-11-10
CA2210584A1 (en) 1996-08-15
US5556771A (en) 1996-09-17
DE69628959T2 (de) 2004-05-13
AU699590B2 (en) 1998-12-10
US5614387A (en) 1997-03-25
EP0726310A1 (de) 1996-08-14

Similar Documents

Publication Publication Date Title
DE69628959D1 (de) Stabilisierte Zusammensetzungen von Reverser Transkriptase und RNA Polymerase für Nukleinsäureamplifikation
EP2272952B8 (de) Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
DE69613856D1 (de) Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
DE69907240D1 (de) Feste phytase-zusammensetzungen
ES2186293T3 (es) Metodo para la amplificacion exponencial directa y secuenciacion de moleculas de dna y su aplicacion.
EP1050587A3 (de) Erzeugung und Amplifizierung von Nukleinsäuren ausgehend von Ribonukleinsäuren
CA2222744A1 (en) Thermostable dna polymerases
CA2335153A1 (en) Enzyme stabilization by cationic surfactants
EP0975806A4 (de) Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)
DE69534855D1 (de) Enzymaktive dna moleküle
DE69523360D1 (de) Isotherme Amplifikation von Nukleinsäuren durch Strangverdrängung
CA2135073A1 (en) Nucleic acid sequence amplification method, composition and kit
WO1995004162A3 (en) Dna sequencing enzymes
DK0550696T3 (da) Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus
FI960540A0 (fi) Eksonukleaasin ja/tai glykosylaasin käyttö antipolymeraasivasta-aineenlisäaineina spesifisyyden lisäämiseksi polymeraasiketjureaktiossa
WO1995027067A1 (en) Purified dna polymerase from bacillus stearothermophilus
DE60022541D1 (de) Polynukleotid amplifizierungsverfahren
CA2158486A1 (en) Coamplification of target nucleic acids using volume exclusion agent, in reaction composition, test kit and test device useful therefor
CA2273078A1 (en) Use of nucleic acids bound to carrier macromolecules
ATE198083T1 (de) Thermostable nukleinsäure polymerase
CA2300071A1 (en) Novel compounds for generating chemiluminescence with a peroxidase
EP1312684A3 (de) Quantitative Multiplex-PCR mit grossem Dynamikbereich
EP1064296A4 (de) Temperaturstabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus
Sun et al. Pyrene nucleotide as a mechanistic probe: evidence for a transient abasic site-like intermediate in the bypass of dipyrimidine photoproducts by T7 DNA polymerase
WO2003027258A3 (en) RECOMBINANT DNA PROCESSES USING A dNTP MIXTURE CONTAINING MODIFIED NUCLEOTIDES

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0726310

Country of ref document: EP

REN Ceased due to non-payment of the annual fee