DE69523360D1 - Isotherme Amplifikation von Nukleinsäuren durch Strangverdrängung - Google Patents

Isotherme Amplifikation von Nukleinsäuren durch Strangverdrängung

Info

Publication number
DE69523360D1
DE69523360D1 DE69523360T DE69523360T DE69523360D1 DE 69523360 D1 DE69523360 D1 DE 69523360D1 DE 69523360 T DE69523360 T DE 69523360T DE 69523360 T DE69523360 T DE 69523360T DE 69523360 D1 DE69523360 D1 DE 69523360D1
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DE
Germany
Prior art keywords
primers
nucleic acids
strand displacement
isothermal amplification
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69523360T
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English (en)
Other versions
DE69523360T2 (de
Inventor
Nanibhushan Dattagupta
Paul Douglas Stull
Marc Spingola
Daniel Louis Kacian
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Gen Probe Inc
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Gen Probe Inc
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Publication of DE69523360D1 publication Critical patent/DE69523360D1/de
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Publication of DE69523360T2 publication Critical patent/DE69523360T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69523360T 1994-03-16 1995-03-16 Isotherme Amplifikation von Nukleinsäuren durch Strangverdrängung Expired - Lifetime DE69523360T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US21508194A 1994-03-16 1994-03-16

Publications (2)

Publication Number Publication Date
DE69523360D1 true DE69523360D1 (de) 2001-11-29
DE69523360T2 DE69523360T2 (de) 2002-07-11

Family

ID=22801567

Family Applications (1)

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DE69523360T Expired - Lifetime DE69523360T2 (de) 1994-03-16 1995-03-16 Isotherme Amplifikation von Nukleinsäuren durch Strangverdrängung

Country Status (11)

Country Link
US (4) US6087133A (de)
EP (1) EP0676476B1 (de)
JP (1) JPH09510351A (de)
KR (1) KR100230718B1 (de)
AT (1) ATE207543T1 (de)
AU (1) AU687535B2 (de)
CA (1) CA2185239C (de)
DE (1) DE69523360T2 (de)
DK (1) DK0676476T3 (de)
ES (1) ES2164112T3 (de)
WO (1) WO1995025180A1 (de)

Families Citing this family (309)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2185239C (en) * 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification
US5514551A (en) * 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
AT402203B (de) * 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
EP0796347A2 (de) * 1995-09-21 1997-09-24 Becton, Dickinson and Company Nachweis von nukleinsäure in zelle unter verwendung von thermophilen strangverdrängungsamplifikation
CA2286304C (en) 1997-04-10 2007-08-07 Diagnocure Inc. Pca3, pca3 genes, and methods of use
GB9717061D0 (en) * 1997-08-13 1997-10-15 Tepnel Medical Ltd Amplification of nucleic acids
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
WO2000004193A1 (en) 1998-07-20 2000-01-27 Yale University Method for detecting nucleic acids using target-mediated ligation of bipartite primers
EP1114184A2 (de) 1998-09-15 2001-07-11 Yale University Klonierung mittels "rolling circle" amplifizierung
US6255082B1 (en) 1998-09-15 2001-07-03 Yale University Artificial long terminal repeat vectors
EP1222266B1 (de) 1999-09-29 2006-03-29 Diagnocure Inc. Pca3 mrna in gutartigen und bösartigen prostatageweben
WO2001077317A1 (en) * 2000-04-07 2001-10-18 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
US20060166227A1 (en) * 2000-06-20 2006-07-27 Stephen Kingsmore Protein expression profiling
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
DE60045932D1 (de) 2000-09-01 2011-06-16 Gen Probe Inc HIV-1-Sequenzverstärkung zur Erkennung von Sequenzen in Zusammenhang mit Wirkstoffresistenz-Mutationen
JP4092201B2 (ja) * 2000-12-26 2008-05-28 タカラバイオ株式会社 病原微生物の検出方法
KR20030071854A (ko) * 2001-02-06 2003-09-06 다카라 바이오 가부시키가이샤 증폭 핵산 및 그의 고정화물
EP1392866B1 (de) * 2001-05-14 2012-03-28 Henry Hongjun Ji Neues verfahren zur klonierung von sequenzen variabler domänen des immunologischen genrepertoires
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
JPWO2002101042A1 (ja) * 2001-06-12 2005-04-07 タカラバイオ株式会社 核酸増幅又は検出反応用試薬の安定化方法ならびに保存方法
US7118864B2 (en) * 2001-06-15 2006-10-10 Quiatech Ab Amplifiable probe
CA2492220C (en) 2001-07-15 2014-03-18 Keck Graduate Institute Nucleic acid amplification using nicking agents
WO2004022701A2 (en) * 2001-07-15 2004-03-18 Keck Graduate Institute Exponential amplification of nucleic acids using nicking agents
AU2002318253A1 (en) 2001-07-15 2003-03-03 Keck Graduate Institute Methylation analysis using nicking agents
KR20040028991A (ko) 2001-08-20 2004-04-03 다카라 바이오 가부시키가이샤 핵산 증폭 방법
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6977148B2 (en) * 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
EP1481088B1 (de) * 2002-03-01 2008-09-17 Integrated Dna Technologies, Inc. Polynomiale amplifikation von nukleinsäuren
US7629152B2 (en) * 2002-03-01 2009-12-08 Integrated Dna Technologies, Inc. Methods for amplifying polymeric nucleic acids
US7405062B2 (en) * 2002-05-14 2008-07-29 San Diego Antibody Method for cloning variable domain sequences of immunological gene repertoire
WO2004027025A2 (en) * 2002-09-20 2004-04-01 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
EP1567675A4 (de) 2002-11-21 2006-05-10 Epict Technologies Verfahren zur verwendung von primern,die für einen strang eines doppelsträngigen promotors codieren
JP4185763B2 (ja) * 2002-12-12 2008-11-26 富士フイルム株式会社 ターゲット核酸の定量方法
WO2004058987A2 (en) 2002-12-20 2004-07-15 Qiagen Gmbh Nucleic acid amplification
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
CA2513780C (en) 2003-02-07 2014-12-30 Diagnocure Inc. Method to detect prostate cancer from a urine sample
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
EP1633893B1 (de) 2003-05-19 2012-01-25 Gen-Probe Incorporated Zusammensetzungen, verfahren und kits zur bestimmung des vorhandenseins von trichomonas vaginalis in einer testprobe
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
US20050053980A1 (en) 2003-06-20 2005-03-10 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20040259100A1 (en) * 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20050069926A1 (en) * 2003-08-01 2005-03-31 Affymetrix, Inc. Helicase-amplified reverse transcription
KR100968901B1 (ko) 2003-12-10 2010-07-14 다카라 바이오 가부시키가이샤 핵산의 증폭방법
US7314714B2 (en) 2003-12-19 2008-01-01 Affymetrix, Inc. Method of oligonucleotide synthesis
TW200525026A (en) 2003-12-25 2005-08-01 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
CA2558266C (en) 2004-03-05 2017-10-17 Gen-Probe Incorporated Reagents, methods and kits for use in deactivating nucleic acids
WO2005113816A2 (en) 2004-05-07 2005-12-01 Henry M. Jackson Foundation For The Advancement Of Military Medicine Methods of diagnosing or treating prostate cancer using the erg gene, alone or in combination with other over or under expressed genes in prostate cancer
EP1751314B1 (de) 2004-05-13 2019-09-18 Nanobiosym, Inc. Nano-pcr: verfahren und vorrichtung zur amplifikation und detektion von nukleinsäuren
GB0416944D0 (en) * 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
US20060216724A1 (en) * 2004-07-30 2006-09-28 Affymetrix, Inc. Methods for normalized amplification of nucleic acids
US20060275792A1 (en) * 2004-11-15 2006-12-07 Lee Jun E Enhancement of nucleic acid amplification using double-stranded DNA binding proteins
CA2491067A1 (en) 2004-12-24 2006-06-24 Stichting Katholieke Universiteit Mrna rations in urinary sediments and/or urine as a prognostic marker for prostate cancer
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
US20100015654A1 (en) * 2005-05-16 2010-01-21 Institut De Recherches Cliniques De Montreal/ I.R.C.M. Negative regulation of NK cell functions by EAT-2, a sap-related adaptor expressed in innate immune cells
ATE550441T1 (de) * 2005-09-06 2012-04-15 Gen Probe Inc Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP2377929A1 (de) 2005-10-06 2011-10-19 Lucigen Corporation Wärmestabile virale Polymerasen und Verwendungsverfahren
KR100816419B1 (ko) * 2005-10-14 2008-03-27 래플진(주) 핵산의 등온증폭 방법 및 핵산과 신호 프로브의 동시등온증폭을 이용한 핵산의 검출방법
US20080057499A1 (en) * 2006-02-06 2008-03-06 Affymetrix, Inc. Methods for high specificity whole genome amplification and hybridization
CN101479605A (zh) * 2006-04-21 2009-07-08 纳诺拜希姆公司 用于药物发现的单分子平台：用于包括抗癌和抗病毒剂的发现的药物发现的方法和装置
EP2021506B1 (de) * 2006-04-28 2016-03-16 Igor Kutyavin Verwendung von pcr-amplifikationsprodukten mit elementen sekundärer struktur für verbesserten pcr-basierten nukleinsäurenachweis
AU2007244658B2 (en) 2006-04-28 2014-02-13 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates
DE102006020885A1 (de) * 2006-05-05 2007-11-08 Qiagen Gmbh Einführung von Sequenzelementen in Nukleinsäuren
US7501254B2 (en) 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
US8980561B1 (en) 2006-08-22 2015-03-17 Los Alamos National Security, Llc. Nucleic acid detection system and method for detecting influenza
WO2008105814A2 (en) 2006-08-22 2008-09-04 Los Alamos National Security, Llc Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids
AU2008206221A1 (en) * 2007-01-17 2008-07-24 Meridian Bioscience, Inc. Stable reagents and kits useful in loop-mediated isothermal amplification (LAMP)
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
US8153064B2 (en) 2007-03-22 2012-04-10 Doebler Ii Robert W Systems and devices for isothermal biochemical reactions and/or analysis
CN103952470B (zh) 2007-03-28 2017-11-10 信号诊断公司 高解析度分析核酸以检测序列变异的系统和方法
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
EP2232259B1 (de) 2008-01-09 2016-10-12 Keck Graduate Institute System, vorrichtung und verfahren zur materialvorbereitung und/oder -handhabung
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
CN102084238B (zh) 2008-05-05 2016-06-01 洛斯阿拉莫斯国家安全有限责任公司 基于高度简化的侧向流动的核酸样品制备和被动流体流动控制
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2010048605A1 (en) 2008-10-24 2010-04-29 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
AU2010208386B2 (en) 2009-01-27 2016-08-11 Qiagen Gaithersburg Thermophilic helicase dependent amplification technology with endpoint homogenous fluorescent detection
WO2010091111A1 (en) 2009-02-03 2010-08-12 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
DK2408905T3 (en) 2009-03-16 2017-08-28 Pangu Biopharma Ltd Compositions and Methods comprising Histidyl-tRNA Synthetase Splicing Variants with Non-Canonical Biological Activities
WO2010120509A2 (en) 2009-03-31 2010-10-21 Atyr Pharma, Inc. Compositions and methods comprising aspartyl-trna synthetases having non-canonical biological activities
JP5711227B2 (ja) 2009-06-26 2015-04-30 クレアモント バイオソリューソンズ エルエルシー 試料破壊用ビーズを利用した生物検体の捕捉および溶出
EP2531515B1 (de) 2010-01-22 2015-10-21 Gen-Probe Incorporated Sonden zur erkennung des vorhandenseins von trichomonas vaginalis in einer probe
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
PT2556171E (pt) 2010-04-05 2015-12-21 Prognosys Biosciences Inc Ensaios biológicos codificados espacialmente
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
EP2563380B1 (de) 2010-04-26 2018-05-30 aTyr Pharma, Inc. Innovative entdeckung therapeutischer, diagnostischer und antikörperhaltiger zusammensetzungen im zusammenhang mit proteinfragmenten der cysteinyl-trna-synthetase
JP6294074B2 (ja) 2010-04-27 2018-03-14 エータイアー ファーマ， インコーポレイテッド イソロイシルｔＲＮＡ合成酵素のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
US8993723B2 (en) 2010-04-28 2015-03-31 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of alanyl-tRNA synthetases
CA2800557A1 (en) 2010-04-29 2011-11-03 Medical Prognosis Institute A/S Methods and devices for predicting treatment efficacy
US9068177B2 (en) 2010-04-29 2015-06-30 Atyr Pharma, Inc Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of glutaminyl-tRNA synthetases
CA2797393C (en) 2010-04-29 2020-03-10 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of valyl trna synthetases
AU2011248490B2 (en) 2010-04-29 2016-11-10 Pangu Biopharma Limited Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of Asparaginyl tRNA synthetases
CN103140233B (zh) 2010-05-03 2017-04-05 Atyr 医药公司 与甲硫氨酰‑tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的发现
WO2011139986A2 (en) 2010-05-03 2011-11-10 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of arginyl-trna synthetases
WO2011140132A2 (en) 2010-05-03 2011-11-10 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of phenylalanyl-alpha-trna synthetases
JP6008844B2 (ja) 2010-05-04 2016-10-19 エータイアー ファーマ， インコーポレイテッド ｐ３８ＭＵＬＴＩ−ｔＲＮＡ合成酵素複合体のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
JP6396656B2 (ja) 2010-05-14 2018-09-26 エータイアー ファーマ， インコーポレイテッド フェニルアラニルβｔＲＮＡ合成酵素のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
WO2011143611A2 (en) 2010-05-14 2011-11-17 Life Technologies Corporation Karyotyping assay
JP6027965B2 (ja) 2010-05-17 2016-11-16 エータイアー ファーマ， インコーポレイテッド ロイシルｔＲＮＡ合成酵素のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
DK2576577T3 (en) 2010-05-28 2015-04-20 Life Technologies Corp Synthesis of 2 ', 3'-dideoxynucleosides for automated DNA synthesis and PYROPHOSPHOROLYSIS ACTIVATED POLYMERIZATION
JP5906237B2 (ja) 2010-06-01 2016-04-20 エータイアー ファーマ， インコーポレイテッド リジルｔＲＮＡ合成酵素のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
JP6116479B2 (ja) 2010-07-12 2017-04-19 エータイアー ファーマ， インコーポレイテッド グリシルｔＲＮＡ合成酵素のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
US9175354B2 (en) 2010-08-03 2015-11-03 Life Technologies Corporation Detection of Salmonella enterica subspecies enterica serovar Enteritidis in food and environmental samples, methods and compositions therefor
EP2420579A1 (de) 2010-08-17 2012-02-22 QIAGEN GmbH Helikasenabhängige isothermale Amplifikation mit Nicking-Enzymen
CA2808539C (en) 2010-08-25 2021-05-25 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of tyrosyl-trna synthetases
WO2012044956A1 (en) * 2010-10-01 2012-04-05 Ibis Biosciences, Inc. Targeted genome amplification methods
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2663639B1 (de) 2011-01-14 2017-09-13 Life Technologies Corporation Verfahren zur isolierung, identifikation und quantifizierung von mirnas
EP2665833B1 (de) 2011-01-17 2017-04-19 Life Technologies Corporation Arbeitsablauf zur nachweis von liganden mit nukleinsäuren
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
KR20140044323A (ko) 2011-04-20 2014-04-14 메사 테크 인터내셔널, 인코포레이티드 핵산의 왕복 증폭 반응
WO2012159025A2 (en) 2011-05-18 2012-11-22 Life Technologies Corporation Chromosome conformation analysis
US20140106986A1 (en) 2011-06-01 2014-04-17 Medical Prognosis Institute A/S Methods and devices for prognosis of cancer relapse
WO2012170908A1 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Design and development of novel detergents for use in pcr systems
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
US9586987B2 (en) 2011-09-08 2017-03-07 Kabushiki Kaisha Dnaform Primer set for isothermal amplication of a target nucleic acid sequence
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
EP2814514B1 (de) 2012-02-16 2017-09-13 Atyr Pharma, Inc. Histidyl-trna-synthetasen zur behandlung von autoimmun- und entzündungserkrankungen
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
EP2861757B1 (de) 2012-06-14 2019-11-06 Life Technologies Corporation Neuartige zusammensetzungen, verfahren und kits für polymerasekettenreaktionen (pcr)
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
EP3428290B1 (de) 2012-07-26 2022-04-06 Illumina, Inc. Zusammensetzungen und verfahren zur amplifikation von nukleinsäuren
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9181583B2 (en) 2012-10-23 2015-11-10 Illumina, Inc. HLA typing using selective amplification and sequencing
WO2014071070A1 (en) * 2012-11-01 2014-05-08 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
US9416405B2 (en) 2012-11-02 2016-08-16 Life Technologies Corporation Compositions, methods and kits for enhancing PCR specificity
EP2914618B1 (de) 2012-11-02 2017-07-26 Novartis Tiergesundheit AG Mit 'theiler's disease' assoziiertes flavivirus
WO2014085434A1 (en) 2012-11-27 2014-06-05 Pontificia Universidad Catolica De Chile Compositions and methods for diagnosing thyroid tumors
WO2014093330A1 (en) 2012-12-10 2014-06-19 Clearfork Bioscience, Inc. Methods for targeted genomic analysis
US9074248B1 (en) 2012-12-18 2015-07-07 Qiagen Gmbh Primers for helicase dependent amplification and their methods of use
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US11254977B2 (en) 2013-03-12 2022-02-22 Life Technologies Corporation Universal reporter-based genotyping methods and materials
WO2014142850A1 (en) 2013-03-13 2014-09-18 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2014142841A1 (en) 2013-03-13 2014-09-18 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
WO2014140167A1 (en) 2013-03-14 2014-09-18 Dsm Ip Assets B.V. Cell wall deconstruction enzymes of malbranchea cinnamomea and uses thereof
WO2014140165A1 (en) 2013-03-14 2014-09-18 Dsm Ip Assets B.V. Cell wall deconstruction enzymes of paecilomyces byssochlamydoides and uses thereof
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
US10392667B2 (en) 2013-06-07 2019-08-27 Medical Prognosis Institute A/S Methods and devices for predicting treatment efficacy of fulvestrant in cancer patients
DK3013984T3 (da) 2013-06-25 2023-06-06 Prognosys Biosciences Inc Metode til bestemmelse af spatiale mønstre i biologiske targets i en prøve
PT3017065T (pt) 2013-07-01 2018-12-18 Illumina Inc Funcionalização de superfícies sem catalisador e enxerto de polímeros
GB2546833B (en) 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
US9155992B2 (en) 2013-09-16 2015-10-13 Savannah River Nuclear Solutions, Llc Mass transfer apparatus and method for separation of gases
ES2925313T3 (es) 2013-10-09 2022-10-14 Fluoresentric Inc Método para la detección de secuencias de ácido nucleico diana
WO2015061714A1 (en) 2013-10-25 2015-04-30 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
EP3068896B1 (de) 2013-11-12 2018-08-08 Life Technologies Corporation Reagenzien und verfahren zur sequenzierung
AU2014364180B2 (en) 2013-12-09 2021-03-04 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
WO2015095226A2 (en) 2013-12-20 2015-06-25 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic dna samples
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
KR102001554B1 (ko) 2014-01-16 2019-07-18 일루미나, 인코포레이티드 고형 지지체 상에서의 앰플리콘 제조 방법 및 시퀀싱
WO2015152024A1 (ja) 2014-03-31 2015-10-08 独立行政法人理化学研究所 蛍光性標識一本鎖核酸及びその用途
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
GB201410420D0 (en) 2014-06-11 2014-07-23 Illumina Cambridge Ltd Methods for estimating cluster numbers
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
WO2016003814A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
US11214836B2 (en) 2014-07-15 2022-01-04 Ontario Institute For Cancer Research Methods and devices for predicting anthracycline treatment efficacy
KR101974915B1 (ko) 2014-08-04 2019-05-03 젠셀 바이오시스템즈 리미티드 게놈 분석 장치 및 사용 방법
EP3183577B1 (de) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible oberflächenfunktionalisierung
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
WO2016061111A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, kits & compositions for determining gene copy numbers
RU2709655C2 (ru) 2014-10-17 2019-12-19 Иллумина Кембридж Лимитед Транспозиция с сохранением сцепления генов
CN107108822B (zh) 2014-10-31 2020-02-07 伊鲁米纳剑桥有限公司 聚合物以及dna共聚物涂层
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
EP3218513B1 (de) 2014-11-11 2018-10-31 Illumina, Inc. Polynukleotidamplifikation mit crispr-cas-systemen
KR102657306B1 (ko) 2014-12-08 2024-04-12 버그 엘엘씨 전립선암의 진단 및 치료에서 필라민을 포함하는 마커의 용도
CN107429296B (zh) 2015-03-13 2022-01-28 生命技术公司 捕获、检测和定量小rna的方法、组合物与试剂盒
EP3277839B1 (de) 2015-03-31 2020-12-02 Illumina Cambridge Limited Oberfläche für conkatemerization von matrizen
FI3901281T3 (fi) 2015-04-10 2023-01-31 Biologisten näytteiden spatiaalisesti eroteltu moninkertainen nukleiinihappoanalyysi
CN118788410A (zh) 2015-04-24 2024-10-18 美飒生物技术公司 流体检测盒
DK3822365T3 (da) 2015-05-11 2023-02-06 Illumina Inc Platform til opdagelse og analyse af terapeutiske midler
EP3878974A1 (de) 2015-07-06 2021-09-15 Illumina Cambridge Limited Probenvorbereitung zur nukleinsäureamplifikation
US10808282B2 (en) 2015-07-07 2020-10-20 Illumina, Inc. Selective surface patterning via nanoimprinting
EP3325648B1 (de) 2015-07-17 2023-03-29 Illumina, Inc. Polymerfolien zur sequenzierung von anwendungen
EP3344389B1 (de) 2015-09-02 2020-06-10 Illumina Cambridge Limited Verfahren zur reparatur von defekten in einer hydrophobischen oberfläche eines tröpfchen-aktuators
DK3377226T3 (da) 2016-03-28 2021-04-26 Illumina Inc Mikrorækker i flere niveauer
EP4269611A3 (de) 2016-05-11 2024-01-17 Illumina, Inc. Polynukleotidanreicherung und -amplifikation unter verwendung von argonautensystemen
EP3458913B1 (de) 2016-05-18 2020-12-23 Illumina, Inc. Selbstassemblierte strukturierung mit gemusterten hydrophoben oberflächen
WO2017205957A1 (en) 2016-06-01 2017-12-07 9087-4405 Quebec Inc. Remote access system and method for plant pathogen management
EP3472349A1 (de) 2016-06-16 2019-04-24 Life Technologies Corporation Neuartige zusammensetzungen, verfahren und kits zum mikroorganismusnachweis
US11535883B2 (en) 2016-07-22 2022-12-27 Illumina, Inc. Single cell whole genome libraries and combinatorial indexing methods of making thereof
CN118207299A (zh) 2016-08-26 2024-06-18 生命技术公司 核酸提取和扩增对照及其使用方法
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
EP4095263A1 (de) 2017-01-06 2022-11-30 Editas Medicine, Inc. Verfahren zur beurteilung der nukleasespaltung
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
WO2018197945A1 (en) 2017-04-23 2018-11-01 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
CA3059952C (en) 2017-04-23 2023-04-18 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
CA3060979C (en) 2017-04-23 2023-07-11 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
US11572591B2 (en) 2017-04-26 2023-02-07 The Translational Genomics Research Institute Methods and assays for subtyping Staphylococcus aureus clonal complex 8 strains
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
KR102628035B1 (ko) 2017-06-07 2024-01-22 오레곤 헬스 앤드 사이언스 유니버시티 메틸화 서열결정을 위한 단일 세포 전체 게놈 라이브러리
WO2019027767A1 (en) 2017-07-31 2019-02-07 Illumina Inc. SEQUENCING SYSTEM COMPRISING AGGREGATION OF MULTIPLEXED BIOLOGICAL SAMPLES
US11352668B2 (en) 2017-08-01 2022-06-07 Illumina, Inc. Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
NZ759924A (en) 2017-08-01 2023-07-28 Illumina Inc Hydrogel beads for nucleotide sequencing
US10858701B2 (en) 2017-08-15 2020-12-08 Omniome, Inc. Scanning apparatus and method useful for detection of chemical and biological analytes
CA3079411C (en) 2017-10-19 2023-12-05 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
US20200340041A1 (en) 2017-11-13 2020-10-29 Life Technologies Corporation Novel compositions, methods and kits for urinary tract microorganism detection
US10724083B1 (en) 2017-12-06 2020-07-28 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable oligonucleotide ligands
US10724017B1 (en) 2017-12-06 2020-07-28 Cepheid Inhibition of DNA polymerases by uracil-DNA glycosylase-cleavable oligonucleotide ligands
US10689629B1 (en) 2017-12-06 2020-06-23 Cepheid Inhibition of nucleic acid polymerases by endonuclease V-cleavable circular oligonucleotide ligands
DK3752634T3 (da) 2018-02-13 2022-10-24 Illumina Inc DNA Sequencing Using Hydrogel Beads / DNA-sekventering ved anvendelse af hydrogelkorn
WO2019183188A1 (en) 2018-03-22 2019-09-26 Illumina, Inc. Preparation of nucleic acid libraries from rna and dna
JP6974504B2 (ja) 2018-04-02 2021-12-01 イルミナ インコーポレイテッド 配列に基づく遺伝子試験のための対照を作製するための組成物及び方法
US20190338352A1 (en) 2018-04-19 2019-11-07 Omniome, Inc. Accuracy of base calls in nucleic acid sequencing methods
CA3067181A1 (en) 2018-04-20 2019-10-24 Illumina, Inc. Methods of encapsulating single cells, the encapsulated cells and uses thereof
BR112020005982A2 (pt) 2018-05-17 2020-12-08 Illumina, Inc. Sequenciamento de células únicas com alto rendimento com viés de amplificação reduzido
SG11202000905PA (en) 2018-06-04 2020-02-27 Illumina Inc High-throughput single-cell transcriptome libraries and methods of making and of using
US20210310059A1 (en) 2018-08-08 2021-10-07 Gen-Probe Incorporated Compositions, methods and kits for detecting mycoplasma genitalium
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020086843A1 (en) 2018-10-26 2020-04-30 Illumina, Inc. Modulating polymer beads for dna processing
US20240011086A1 (en) 2018-11-15 2024-01-11 Omniome, Inc. Electronic detection of nucleic acid structure
EP3887540B1 (de) 2018-11-30 2023-08-23 Illumina, Inc. Analyse von mehreren analyten unter verwendung eines einzigen tests
EP3891304A1 (de) 2018-12-04 2021-10-13 Omniome, Inc. Mischphasenfluide für nukleinsäuresequenzierung und andere analytische tests
EP3894587A1 (de) 2018-12-10 2021-10-20 10X Genomics, Inc. Auflösung von räumlichen anordnungen durch nähebasierte dekonvolution
JP2022512264A (ja) 2018-12-19 2022-02-03 イラミーナ インコーポレーテッド ポリヌクレオチドクラスターのクローナリティを改善するための方法
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3927467A4 (de) 2019-02-20 2022-12-14 Pacific Biosciences of California, Inc. Scanner und verfahren zum nachweis von chemischen und biologischen analyten
US20220033805A1 (en) 2019-03-01 2022-02-03 Sanjay Srivatsan High-throughput single-nuclei and single-cell libraries and methods of making and of using
CA3138566A1 (en) 2019-04-30 2020-11-05 Larimar Therapeutics, Inc. Frataxin-sensitive markers for determining effectiveness of frataxin replacement therapy
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
BR112021012755A2 (pt) 2019-07-12 2022-04-26 Illumina Cambridge Ltd Composições e métodos para preparar bibliotecas de sequenciamento de ácidos nucleicos usando crispr/cas9 imobilizadas em um suporte sólido
JP2022539630A (ja) 2019-07-12 2022-09-13 イルミナ ケンブリッジ リミテッド 電気泳動を用いた核酸ライブラリの調製
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
US20220298548A1 (en) 2019-08-23 2022-09-22 Gen-Probe Incorporated Compositions, methods and kits for detecting treponema pallidum
WO2021046270A1 (en) 2019-09-05 2021-03-11 Gen-Probe Incorporated Detection of chlamydia trachomatis nucleic acid variants
WO2021050681A1 (en) 2019-09-10 2021-03-18 Omniome, Inc. Reversible modification of nucleotides
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
CA3134746A1 (en) 2019-12-19 2021-06-24 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
EP4424843A3 (de) 2019-12-23 2024-09-25 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von rna-template-ligation
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
WO2021158511A1 (en) 2020-02-04 2021-08-12 Omniome, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
AU2021249046A1 (en) 2020-03-30 2022-10-20 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
CN115916999A (zh) 2020-04-22 2023-04-04 10X基因组学有限公司 用于使用靶向rna耗竭进行空间分析的方法
JP2023526062A (ja) 2020-05-12 2023-06-20 イルミナ インコーポレイテッド 組換え末端デオキシヌクレオチジルトランスフェラーゼを使用して修飾塩基を有する核酸の生成
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4414459A3 (de) 2020-05-22 2024-09-18 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
AU2021283184A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
EP4025692A2 (de) 2020-06-02 2022-07-13 10X Genomics, Inc. Nukleinsäure-bibliotheksverfahren
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
EP4165207B1 (de) 2020-06-10 2024-09-25 10X Genomics, Inc. Verfahren zur bestimmung der position eines analyten in einer biologischen probe
EP4450639A2 (de) 2020-06-25 2024-10-23 10X Genomics, Inc. Räumliche analyse der dna-methylierung
EP4172364A1 (de) 2020-06-30 2023-05-03 Illumina, Inc. Katalytisch gesteuerte sequenzierung durch synthese zur herstellung von narbenfreier dna
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
KR20230056683A (ko) 2020-07-23 2023-04-27 라이프 테크놀로지스 코포레이션 생물학적 분석에 사용하기 위한 에너지 전달 염료 접합체
US20230304932A1 (en) 2020-07-23 2023-09-28 Life Technologies Corporation Compositions, systems and methods for biological analysis involving energy transfer dye conjugates and analytes comprising the same
IL300112A (en) 2020-08-06 2023-03-01 Illumina Inc Preparation of RNA and DNA sequencing libraries using bead-linked transpososomes
WO2022040176A1 (en) 2020-08-18 2022-02-24 Illumina, Inc. Sequence-specific targeted transposition and selection and sorting of nucleic acids
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
WO2022047359A1 (en) 2020-08-31 2022-03-03 Berg Llc Protein biomarkers for pancreatic cancer
WO2022053610A1 (en) 2020-09-11 2022-03-17 Illumina Cambridge Limited Methods of enriching a target sequence from a sequencing library using hairpin adaptors
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
MX2023009046A (es) 2021-02-04 2023-08-10 Illumina Inc Generacion de lectura unida indexada prolongada en globulos unidos con transposomas.
WO2022178267A2 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. Modular assay support devices
AU2022232600A1 (en) 2021-03-09 2023-09-14 Illumina, Inc. Analyzing expression of protein-coding variants in cells
CA3209074A1 (en) 2021-03-09 2022-09-15 Andrew Kennedy Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins
EP4301870A1 (de) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
CN117222747A (zh) 2021-03-22 2023-12-12 伊鲁米纳剑桥有限公司 用于改善核酸簇克隆性的方法
EP4314328A1 (de) 2021-03-29 2024-02-07 Illumina, Inc. Zusammensetzungen und verfahren zur beurteilung von dna-schäden in einer bibliothek und zur normalisierung des amplicongrössenbias
JP2024511766A (ja) 2021-03-29 2024-03-15 イルミナ インコーポレイテッド 改善されたライブラリ調製方法
IL307172A (en) 2021-03-30 2023-11-01 Illumina Inc Improved methods of isothermal complementary DNA and library preparation
JP2024511760A (ja) 2021-03-31 2024-03-15 イルミナ インコーポレイテッド エラー補正のための固有分子識別子を有するトランスポゾンベースの技術を使用した指向性タグメンテーション配列決定ライブラリーの調製方法
IL307531A (en) 2021-04-06 2023-12-01 Bpgbio Inc Protein markers for estrogen receptor (ER)-like and estrogen receptor (ER)-negative breast cancer
EP4320440A1 (de) 2021-04-06 2024-02-14 BPGbio, Inc. Proteinmarker für östrogenrezeptor (er)-positiven luminalen a(la)-ähnlichen und luminalen b1 (lb1)-ähnlichen brustkrebs
IL307528A (en) 2021-04-06 2023-12-01 Bpgbio Inc Protein markers for the prognosis of breast cancer progression
AU2022273052A1 (en) 2021-05-14 2023-11-30 Gen-Probe Incorporated Compositions and methods for detecting human adenovirus nucleic acid
EP4347879A1 (de) 2021-06-03 2024-04-10 10X Genomics, Inc. Verfahren, zusammensetzungen, kits und systeme zur verbesserung der analyterfassung zur räumlichen analyse
US20230016633A1 (en) 2021-06-15 2023-01-19 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11220707B1 (en) 2021-06-17 2022-01-11 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
CN117813391A (zh) 2021-07-23 2024-04-02 因美纳有限公司 制备用于dna测序的基底表面的方法
CA3223731A1 (en) 2021-08-11 2023-02-16 Illumina, Inc. Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation
EP4196605A1 (de) 2021-09-01 2023-06-21 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur blockierung einer erfassungssonde auf einer räumlichen anordnung
WO2023069927A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
WO2023141154A1 (en) 2022-01-20 2023-07-27 Illumina Cambridge Limited Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
WO2023196937A1 (en) 2022-04-06 2023-10-12 Larimar Therapeutics, Inc. Frataxin-sensitive markers for monitoring frataxin replacement therapy
CA3223722A1 (en) 2022-04-07 2023-10-12 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2023240201A1 (en) 2022-06-08 2023-12-14 Larimar Therapeutics, Inc. Frataxin-sensitive markers for monitoring progression and treatment of leigh syndrome
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024129969A1 (en) 2022-12-14 2024-06-20 Illumina, Inc. Systems and methods for capture and enrichment of clustered beads on flow cell substrates

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4786600A (en) * 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
CA1340843C (en) * 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
CA1323293C (en) * 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
CA2013317A1 (en) * 1989-04-17 1990-10-17 Fred T. Oakes Diagnostic kit, primer composition and their use for replication or detection of nucleic acids
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
JPH05500799A (ja) * 1989-06-05 1993-02-18 ギリアド　サイエンシス，インコーポレイテッド エキソヌクレアーゼ抵抗性オリゴヌクレオチドおよびその調製方法
US5256775A (en) * 1989-06-05 1993-10-26 Gilead Sciences, Inc. Exonuclease-resistant oligonucleotides
ATE282716T1 (de) * 1989-07-11 2004-12-15 Gen Probe Inc Verfahren zur amplifikation von nukleinsäuresequenzen
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
NO904633L (no) * 1989-11-09 1991-05-10 Molecular Diagnostics Inc Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe.
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
IE910081A1 (en) * 1990-01-10 1991-07-17 Chiron Corp The use of DNA-dependent RNA polymerase transcripts as¹reporter molecules for signal amplification in nucleic acid¹hybridization assays
US5223414A (en) * 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
AU8997991A (en) * 1991-01-31 1992-08-06 Becton Dickinson & Company Exonuclease mediated strand displacement amplification
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
WO1994003635A1 (en) 1992-08-04 1994-02-17 Institute Of Molecular Biology & Biotechnology Rapid amplification and detection of nucleic acids
AU689789B2 (en) 1993-07-23 1998-04-09 Gen-Probe Incorporated Methods for enhancing nucleic acid amplification
CA2185239C (en) * 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification

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WO1995025180A1 (en) 1995-09-21
US6087133A (en) 2000-07-11
AU2101695A (en) 1995-10-03
CA2185239C (en) 2002-12-17
KR100230718B1 (ko) 1999-11-15
JPH09510351A (ja) 1997-10-21
ATE207543T1 (de) 2001-11-15
EP0676476B1 (de) 2001-10-24
USRE39007E1 (en) 2006-03-07
AU687535B2 (en) 1998-02-26
DK0676476T3 (da) 2001-12-03
USRE38960E1 (en) 2006-01-31
EP0676476A1 (de) 1995-10-11
CA2185239A1 (en) 1995-09-21
ES2164112T3 (es) 2002-02-16
DE69523360T2 (de) 2002-07-11
US6214587B1 (en) 2001-04-10
KR970701791A (ko) 1997-04-12

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