BR112023018157A2 - Análise de expressão de variantes codificantes de proteína em células - Google Patents

Análise de expressão de variantes codificantes de proteína em células

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Publication number
BR112023018157A2
BR112023018157A2 BR112023018157A BR112023018157A BR112023018157A2 BR 112023018157 A2 BR112023018157 A2 BR 112023018157A2 BR 112023018157 A BR112023018157 A BR 112023018157A BR 112023018157 A BR112023018157 A BR 112023018157A BR 112023018157 A2 BR112023018157 A2 BR 112023018157A2
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Brazil
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expression
variant
cells
protein
cell
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BR112023018157A
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English (en)
Inventor
Dan Cao
Hongxia Xu
Kai-How FARH
Mohan Sun
Min Xiao Shi
Tong Liu
Victor Quijano
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Illumina Inc
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Publication of BR112023018157A2 publication Critical patent/BR112023018157A2/pt

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Abstract

análise de expressão de variantes codificantes de proteína em células. a presente invenção refere-se a uma análise de expressão de variantes codificantes de proteína em células. um método pode incluir substituir uma região codificante de proteína do dna em uma célula por um vetor de doador que inclui uma variante da região codificante de proteína e um primeiro código de barras que identifica essa variante. a célula pode gerar mrna que inclui uma expressão da variante e uma expressão do primeiro código de barras. um segundo código de barras que corresponde à célula pode ser acoplado ao mrna. o mrna, que tem o segundo código de barras acoplado a ele, pode ser transcrito re-verso em cdna complementar. o cdna pode ser sequenciado. o vetor de doador ou cdna pode ser sequenciado com o uso de sequenciamento de amplicon. a sequência de vetor de doador e a sequência de cdna podem ser correlacionadas para identificar a variante e a expressão da célula da variante.
BR112023018157A 2021-03-09 2022-03-08 Análise de expressão de variantes codificantes de proteína em células BR112023018157A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202163158492P 2021-03-09 2021-03-09
US202163162775P 2021-03-18 2021-03-18
US202163163381P 2021-03-19 2021-03-19
US202163226424P 2021-07-28 2021-07-28
PCT/US2022/019258 WO2022192191A1 (en) 2021-03-09 2022-03-08 Analyzing expression of protein-coding variants in cells

Publications (1)

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BR112023018157A2 true BR112023018157A2 (pt) 2023-10-31

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US (1) US20240167020A1 (pt)
EP (1) EP4305164A1 (pt)
JP (1) JP2024509446A (pt)
KR (1) KR20230134617A (pt)
AU (1) AU2022232600A1 (pt)
BR (1) BR112023018157A2 (pt)
CA (1) CA3209070A1 (pt)
IL (1) IL305151A (pt)
MX (1) MX2023010649A (pt)
WO (1) WO2022192191A1 (pt)

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US20240167020A1 (en) 2024-05-23
MX2023010649A (es) 2023-09-20
JP2024509446A (ja) 2024-03-01
CA3209070A1 (en) 2022-09-22
KR20230134617A (ko) 2023-09-21
IL305151A (en) 2023-10-01
EP4305164A1 (en) 2024-01-17
WO2022192191A1 (en) 2022-09-15
AU2022232600A1 (en) 2023-09-14

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