BR112021023353A2 - Método de triagem de uma população de células, retrovírus, método de liberação de um ácido nucleico para uma célula, método para detectar uma interação entre um retrovírus e uma célula, biblioteca de retrovírus, e, população de células - Google Patents

Método de triagem de uma população de células, retrovírus, método de liberação de um ácido nucleico para uma célula, método para detectar uma interação entre um retrovírus e uma célula, biblioteca de retrovírus, e, população de células

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Publication number
BR112021023353A2
BR112021023353A2 BR112021023353A BR112021023353A BR112021023353A2 BR 112021023353 A2 BR112021023353 A2 BR 112021023353A2 BR 112021023353 A BR112021023353 A BR 112021023353A BR 112021023353 A BR112021023353 A BR 112021023353A BR 112021023353 A2 BR112021023353 A2 BR 112021023353A2
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Brazil
Prior art keywords
retroviruses
population
cells
cell
retrovirus
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BR112021023353A
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English (en)
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Connor Dobson
Michael Birnbaum
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Massachusetts Inst Technology
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Publication of BR112021023353A2 publication Critical patent/BR112021023353A2/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
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    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
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    • C07K14/52—Cytokines; Lymphokines; Interferons
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    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
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    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
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    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • C—CHEMISTRY; METALLURGY
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
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Abstract

método de triagem de uma população de células, retrovírus, método de liberação de um ácido nucleico para uma célula, método para detectar uma interação entre um retrovírus e uma célula, biblioteca de retrovírus, e, população de células. são descritos aqui composições de retrovírus e métodos de uso das mesmas para liberação de genes, em que os retrovírus compreendem uma proteína de envelope viral que compreende pelo menos uma mutação que diminui sua função nativa, uma proteína ligada à membrana não viral que compreende um domínio ligado à membrana e um domínio de marcação extracelular.
BR112021023353A 2019-05-23 2020-03-23 Método de triagem de uma população de células, retrovírus, método de liberação de um ácido nucleico para uma célula, método para detectar uma interação entre um retrovírus e uma célula, biblioteca de retrovírus, e, população de células BR112021023353A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962851889P 2019-05-23 2019-05-23
PCT/US2020/024175 WO2020236263A1 (en) 2019-05-23 2020-03-23 Ligand discovery and gene delivery via retroviral surface display

Publications (1)

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BR112021023353A2 true BR112021023353A2 (pt) 2022-02-01

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BR112021023353A BR112021023353A2 (pt) 2019-05-23 2020-03-23 Método de triagem de uma população de células, retrovírus, método de liberação de um ácido nucleico para uma célula, método para detectar uma interação entre um retrovírus e uma célula, biblioteca de retrovírus, e, população de células

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US (2) US20200371088A1 (pt)
EP (1) EP3973065A1 (pt)
JP (1) JP2022534085A (pt)
KR (1) KR20220012291A (pt)
CN (1) CN114127305A (pt)
AU (1) AU2020278470A1 (pt)
BR (1) BR112021023353A2 (pt)
CA (1) CA3140115A1 (pt)
IL (1) IL287903A (pt)
MX (1) MX2021014304A (pt)
SG (1) SG11202112344VA (pt)
WO (1) WO2020236263A1 (pt)

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JP2024507996A (ja) * 2021-02-26 2024-02-21 ケロニア セラピューティクス， インコーポレイテッド リンパ球を標的とするレンチウイルスベクター
BR112023021075A2 (pt) * 2021-04-16 2023-12-12 Massachusetts Inst Technology Método para liberar um ou mais ácidos nucleicos a uma célula-tronco hematopoiética, e, método para edição de gene em uma célula-tronco hematopoiética
US20230071148A1 (en) * 2021-07-15 2023-03-09 Vcreate, Inc. Compositions and Methods for Making Novel T-Cell Receptors
WO2023114884A2 (en) * 2021-12-15 2023-06-22 Interius Biotherapeutics, Inc. Pseudotyped viral particles, compositions comprising the same, and uses thereof
WO2023240124A1 (en) * 2022-06-07 2023-12-14 Regeneron Pharmaceuticals, Inc. Pseudotyped viral particles for targeting tcr-expressing cells
GB202211935D0 (en) * 2022-08-16 2022-09-28 Oxford Biomedica Ltd envelope proteins
US20240067958A1 (en) * 2022-08-31 2024-02-29 Gigamune, Inc. Engineered enveloped vectors and methods of use thereof
WO2024050450A1 (en) 2022-08-31 2024-03-07 Gigamune, Inc. Engineered enveloped vectors and methods of use thereof

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US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
ES2348868T3 (es) 2002-12-13 2010-12-16 Genetix Pharmaceuticals Inc. Vectores retrovirales terapeuticos para terapia genica.
EP1975239A1 (en) * 2006-09-27 2008-10-01 Bundesrepublik Deutschland, letztvertreten durch den Präsidenten des Paul-Ehrlich-Instituts Prof. Dr. Johannes Löwer Pseudotyping of retroviral vectors, methods for production and use thereof for targeted gene transfer and high-throughput screening
WO2015104376A1 (en) * 2014-01-10 2015-07-16 Sirion Biotech Gmbh Pseudotyped lentiviral vectors
CA2936352A1 (en) * 2014-01-21 2015-07-30 Albert Einstein College Of Medicine, Inc. Cellular platform for rapid and comprehensive t-cell immunomonitoring
EP3099334B1 (en) 2014-01-30 2019-07-31 Children's Hospital Medical Center An improved fetal hemoglobin for genetic correction of sickle cell disease
US9759689B2 (en) 2014-05-02 2017-09-12 The Regents Of The University Of Michigan Real-time detection and imaging of terahertz pulse radiation by using photoacoustic conversion
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US20200216502A1 (en) * 2017-09-22 2020-07-09 Centre National De La Recherche Scientifique (Cnrs) Mutated Glycoprotein of Vesicular Stomatitis Virus

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WO2020236263A8 (en) 2020-12-24
KR20220012291A (ko) 2022-02-03
US20240044873A1 (en) 2024-02-08
EP3973065A1 (en) 2022-03-30
SG11202112344VA (en) 2021-12-30
AU2020278470A1 (en) 2021-12-02
IL287903A (en) 2022-01-01
JP2022534085A (ja) 2022-07-27
CA3140115A1 (en) 2020-11-26
CN114127305A (zh) 2022-03-01
WO2020236263A1 (en) 2020-11-26
US20200371088A1 (en) 2020-11-26
MX2021014304A (es) 2022-02-21

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