CO2018004763A2 - Edición genómica multiplexada - Google Patents

Edición genómica multiplexada

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Publication number
CO2018004763A2
CO2018004763A2 CONC2018/0004763A CO2018004763A CO2018004763A2 CO 2018004763 A2 CO2018004763 A2 CO 2018004763A2 CO 2018004763 A CO2018004763 A CO 2018004763A CO 2018004763 A2 CO2018004763 A2 CO 2018004763A2
Authority
CO
Colombia
Prior art keywords
cell
nucleic acid
sequences
cas protein
target nucleic
Prior art date
Application number
CONC2018/0004763A
Other languages
English (en)
Inventor
George M Church
Luhan Yang
Marc Guell
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Publication of CO2018004763A2 publication Critical patent/CO2018004763A2/es

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    • A—HUMAN NECESSITIES
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    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
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    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0273—Cloned vertebrates
    • A—HUMAN NECESSITIES
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    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
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    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
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    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C12N15/1132—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses against retroviridae, e.g. HIV
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
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    • A01K2207/00—Modified animals
    • A01K2207/05—Animals modified by non-integrating nucleic acids, e.g. antisense, RNAi, morpholino, episomal vector, for non-therapeutic purpose
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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Abstract

Se proporciona un método para modular algunas o todas las copias de un gen en una célula, que incluye la introducción en una célula de una o más secuencias de ácido ribonucleico (ARN) que comprenden una parte que es complementaria a la totalidad o a una parte de cada una o más de las secuencias de ácido nucleico diana y una secuencia de ácido nucleico que codifica una proteína Cas y que mantiene las células en condiciones en las que se expresa la proteína Cas y la proteína Cas se une y modula las una o más secuencias de ácido nucleico diana en la célula
CONC2018/0004763A 2015-10-08 2018-05-03 Edición genómica multiplexada CO2018004763A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562239239P 2015-10-08 2015-10-08
PCT/US2016/055916 WO2017062723A1 (en) 2015-10-08 2016-10-07 Multiplexed genome editing

Publications (1)

Publication Number Publication Date
CO2018004763A2 true CO2018004763A2 (es) 2018-11-30

Family

ID=58488520

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2018/0004763A CO2018004763A2 (es) 2015-10-08 2018-05-03 Edición genómica multiplexada

Country Status (16)

Country Link
US (5) US10959413B2 (es)
EP (1) EP3359661A4 (es)
JP (4) JP7059468B2 (es)
KR (1) KR20180054871A (es)
CN (2) CN116064664A (es)
AU (2) AU2016335698B2 (es)
BR (1) BR112018007177A2 (es)
CA (1) CA3001314A1 (es)
CL (1) CL2018000902A1 (es)
CO (1) CO2018004763A2 (es)
IL (2) IL297017A (es)
MX (2) MX2018004263A (es)
NZ (1) NZ741778A (es)
SA (1) SA518391294B1 (es)
WO (1) WO2017062723A1 (es)
ZA (1) ZA201802461B (es)

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