BR112018016443A2 - sistema de transposon replicativo - Google Patents

sistema de transposon replicativo

Info

Publication number
BR112018016443A2
BR112018016443A2 BR112018016443A BR112018016443A BR112018016443A2 BR 112018016443 A2 BR112018016443 A2 BR 112018016443A2 BR 112018016443 A BR112018016443 A BR 112018016443A BR 112018016443 A BR112018016443 A BR 112018016443A BR 112018016443 A2 BR112018016443 A2 BR 112018016443A2
Authority
BR
Brazil
Prior art keywords
copy
paste
gene
dna
introducing
Prior art date
Application number
BR112018016443A
Other languages
English (en)
Inventor
Grabundzija Ivana
Buerckstuemmer Tilmann
Vyacheslavovich Kapitonov Vladimir
Ivics Zoltan
Original Assignee
Genetic Information Res Institute
Horizon Discovery Ltd
Max Delbrueck Centrum Fuer Molekulare Medizin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetic Information Res Institute, Horizon Discovery Ltd, Max Delbrueck Centrum Fuer Molekulare Medizin filed Critical Genetic Information Res Institute
Publication of BR112018016443A2 publication Critical patent/BR112018016443A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Reproductive Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

a invenção refere-se a um sistema e método para introduzir dna em células. em particular, a invenção refere-se a um método para introduzir cópias únicas ou múltiplas de uma sequência de dna ou gene de interesse em uma célula compreendendo o fornecimento de: a) uma transposase de ?cópia e colagem?; e b) um construto compreendendo uma sequência de dna ou gene de interesse flanqueado por uma sequência terminal de transposon de ?cópia e colagem?, tais como uma lts ou rts. um novo transposon de ?cópia e colagem? da família helitron é descrito junto com sistemas para usar a transposase correspondente em métodos para introduzir o dna em células, por exemplo, para gerar linhagens celulares para o uso na produção de proteína, célula e terapia genética ou como padrões de referência.
BR112018016443A 2016-02-11 2017-02-10 sistema de transposon replicativo BR112018016443A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1602473.9A GB201602473D0 (en) 2016-02-11 2016-02-11 Replicative transposon system
PCT/GB2017/050355 WO2017137768A1 (en) 2016-02-11 2017-02-10 Replicative transposon system

Publications (1)

Publication Number Publication Date
BR112018016443A2 true BR112018016443A2 (pt) 2019-10-01

Family

ID=55697562

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018016443A BR112018016443A2 (pt) 2016-02-11 2017-02-10 sistema de transposon replicativo

Country Status (11)

Country Link
US (1) US11396664B2 (pt)
EP (1) EP3414333B1 (pt)
JP (1) JP2019504646A (pt)
KR (1) KR20180127339A (pt)
CN (1) CN109072258A (pt)
AU (1) AU2017218651A1 (pt)
BR (1) BR112018016443A2 (pt)
CA (1) CA3011886A1 (pt)
GB (2) GB201602473D0 (pt)
SG (1) SG11201806400XA (pt)
WO (1) WO2017137768A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
US10392616B2 (en) 2017-06-30 2019-08-27 Arbor Biotechnologies, Inc. CRISPR RNA targeting enzymes and systems and uses thereof
WO2019090173A1 (en) * 2017-11-02 2019-05-09 Arbor Biotechnologies, Inc. Novel crispr-associated transposon systems and components
WO2020207560A1 (en) * 2019-04-09 2020-10-15 European Molecular Biology Laboratory Improved transposon insertion sites and uses thereof
CN110257425B (zh) * 2019-05-05 2022-07-15 扬州大学 一种ps转座子系统及其介导的基因转移方法
CN111909986B (zh) * 2019-05-10 2024-03-26 潘雨堃 一种基因治疗安全评估与毒副作用预防的方法
US20220372521A1 (en) * 2019-08-14 2022-11-24 The Trustees Of Columbia University In The City Of New York Rna-guided dna integration and modification
WO2021046243A2 (en) 2019-09-03 2021-03-11 Myeloid Therapeutics, Inc. Methods and compositions for genomic integration
EP4225928A1 (en) * 2020-10-09 2023-08-16 The Broad Institute, Inc. Helitron mediated genetic modification

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6489458B2 (en) 1997-03-11 2002-12-03 Regents Of The University Of Minnesota DNA-based transposon system for the introduction of nucleic acid into DNA of a cell
CN103923932A (zh) 2014-05-07 2014-07-16 重庆大学 具有增强子作用的家蚕BmHel-10转座子
CN104789594A (zh) * 2015-04-27 2015-07-22 江南大学 稳定高效表达人血清白蛋白和白介素ⅱ融合蛋白的cho细胞株的构建方法

Also Published As

Publication number Publication date
JP2019504646A (ja) 2019-02-21
WO2017137768A1 (en) 2017-08-17
EP3414333B1 (en) 2021-12-08
GB201814775D0 (en) 2018-10-24
GB201602473D0 (en) 2016-03-30
CA3011886A1 (en) 2017-08-17
CN109072258A (zh) 2018-12-21
SG11201806400XA (en) 2018-08-30
EP3414333A1 (en) 2018-12-19
US20190323037A1 (en) 2019-10-24
GB2564296A (en) 2019-01-09
KR20180127339A (ko) 2018-11-28
AU2017218651A1 (en) 2018-08-09
US11396664B2 (en) 2022-07-26

Similar Documents

Publication Publication Date Title
BR112018016443A2 (pt) sistema de transposon replicativo
BR112020024863A2 (pt) Nucleases guiadas por rna, fragmentos ativos e variantes das mesmas e métodos de uso
CO2018004763A2 (es) Edición genómica multiplexada
BR112018074325A2 (pt) anticorpo para ligação a receptor de interleucina 4
MX2019003674A (es) Enzimas modificadoras de ácido nucleico guiadas por arn y métodos de uso de estas.
BR112019006388A2 (pt) enzimas modificadoras de ácido nucleico guiadas por rna e métodos de uso das mesmas
WO2018132739A3 (en) T cell receptors that bind to ny-eso-1 and methods of use thereof
BR112018013930A2 (pt) vírus oncolítico, vírus que expressa três genes heterólogos, composição farmacêutica, vírus para o uso, produto de fabricação, método de tratamento de câncer e uso do vírus
ES2690386T3 (es) Composición para escindir un ADN diana que comprende un ARN guía específico para el ADN diana y el ácido nucleico que codifica la proteína Cas o la propia proteína Cas, y sus usos
EP4249036A3 (en) Methods and products for nucleic acid production and delivery
BR112019005144A2 (pt) sistema regulador de expressão, composição, métodos para expressar uma proteína, para induzir/reativar a proliferação de cardiomiócitos neonatais ou adultos e para expressar um gene de interesse, vetor, kit regulador de expressão, e, uso.
AU2016262093A8 (en) Enhancing endonuclease based gene editing in primary cells
BR112013007862A2 (pt) ácidos nucleicos manipulados e métodos de uso dos mesmos.
MX2021010668A (es) Proteinas de fusion de citoquinas.
MX2019002498A (es) Metodos y productos para la expresion de proteinas en celulas.
BR112018067536A2 (pt) sistema de transpóson e métodos de uso
BR112022002695A2 (pt) Nucleases guiadas por rna, fragmentos ativos e variantes das mesmas e métodos de uso
MX2019004487A (es) Metodos y composiciones para el tratamiento de la enfermedad de fabry.
NZ762361A (en) Target sequence specific alteration technology using nucleotide target recognition
BR112018072198A2 (pt) composição compreendendo micrornas, método de preparação de uma composição de célula-tronco restaurada, formulação farmacêutica e kit
WO2020102766A3 (en) Methods for generating circular nucleic acid molecules
BR112017018149A2 (pt) célula hospedeira recombinante, método para produzir uma proteína de interesse recombinante, sistema de expressão, e, processos para fabricar uma formulação de proteína estável e para reduzir a atividade de esterase em uma formulação de proteína.
PH12020550483A1 (en) Anti-apoc3 antibodies and methods of use thereof
Knaupp et al. TINC—a method to dissect regulatory complexes at single-locus resolution—reveals an extensive protein complex at the nanog promoter
IN2013MU02316A (pt)

Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 5A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2657 DE 07-12-2021 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.