BR9805682A - Otimização de células para a ativação endógena de gens. - Google Patents
Otimização de células para a ativação endógena de gens.Info
- Publication number
- BR9805682A BR9805682A BR9805682-4A BR9805682A BR9805682A BR 9805682 A BR9805682 A BR 9805682A BR 9805682 A BR9805682 A BR 9805682A BR 9805682 A BR9805682 A BR 9805682A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Patente de Invenção: <B>"OTIMIZAçãO DE CéLULAS PARA A ATIVAçãO ENDóGENA DE GENS"<D>. A invenção refere-se a um processo para otimização da expressão genética em células. Um primeiro aspecto refere-se a um processo para modificação da expressão de um gene alvo presente endogenamente em uma célula eucarióntica através de introdução de uma seq³ência de controle de expressão heteróloga no genoma da célula por meio de recombinação homóloga, assim como o corte transmitido por uma recombinase de local específico do DNA estranho inserido e sua substituição por outras seq³ências de controle de expressão heterólogas e/ou gens de amplificação. Além disso, a invenção refere-se à introdução de uma ou mais seq³ências de ácido nucléico, na qual uma proteína ativadora ou um complexo de proteína ativadora, por exemplo, encontra um fator induzível de hipoxia (HIF), no genoma de uma célula eucarióntica por recombinação homóloga, para modificar a expressão de um gene alvo. Além disso, a invenção refere-se a um processo para testar a influência dos fragmentos de ácido nucléico não codificadores de lado 5' ou 3' na expressão de um gene alvo por determinação de um gene repórter. Além disso, a invenção refere-se a um processo para preparação de células eucariónticas DHFR negativas contendo uma seq³ência alvo-recombinase assim como a expressão de uma seq³ência de ácido nucléico inserida na seq³ência alvo de recombinase.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP97121075 | 1997-12-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9805682A true BR9805682A (pt) | 2000-04-11 |
Family
ID=8227717
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9805682-4A BR9805682A (pt) | 1997-12-01 | 1998-12-01 | Otimização de células para a ativação endógena de gens. |
Country Status (16)
Country | Link |
---|---|
US (1) | US7008764B1 (pt) |
EP (3) | EP0957165B1 (pt) |
JP (1) | JP4629813B2 (pt) |
KR (1) | KR100367062B1 (pt) |
CN (2) | CN100519751C (pt) |
AR (4) | AR018022A1 (pt) |
AT (3) | ATE320491T1 (pt) |
AU (1) | AU757930B2 (pt) |
BR (1) | BR9805682A (pt) |
CA (1) | CA2252970C (pt) |
DE (3) | DE59813976D1 (pt) |
DK (3) | DK1464705T3 (pt) |
ES (3) | ES2284228T3 (pt) |
PT (3) | PT919619E (pt) |
TR (1) | TR199802503A3 (pt) |
ZA (1) | ZA9810915B (pt) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2278022A3 (en) * | 1999-11-01 | 2011-05-18 | Novartis Vaccines and Diagnostics, Inc. | Expression vectors, transfection systems, and method of use thereof |
WO2001066717A2 (en) * | 2000-03-03 | 2001-09-13 | The University Of Utah | Gene targeting method |
DE10023887A1 (de) * | 2000-05-17 | 2001-11-29 | Axel Haverich | Verfahren zur transienten Insertion genetischer Elemente |
ATE375388T1 (de) * | 2001-07-27 | 2007-10-15 | Us Gov Health & Human Serv | Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden |
AU2003251286B2 (en) | 2002-01-23 | 2007-08-16 | The University Of Utah Research Foundation | Targeted chromosomal mutagenesis using zinc finger nucleases |
WO2004037977A2 (en) | 2002-09-05 | 2004-05-06 | California Institute Of Thechnology | Use of chimeric nucleases to stimulate gene targeting |
US8409861B2 (en) | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
US20070134796A1 (en) * | 2005-07-26 | 2007-06-14 | Sangamo Biosciences, Inc. | Targeted integration and expression of exogenous nucleic acid sequences |
US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US11311574B2 (en) | 2003-08-08 | 2022-04-26 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted cleavage and recombination |
US20120196370A1 (en) | 2010-12-03 | 2012-08-02 | Fyodor Urnov | Methods and compositions for targeted genomic deletion |
US7972854B2 (en) | 2004-02-05 | 2011-07-05 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US20060063231A1 (en) * | 2004-09-16 | 2006-03-23 | Sangamo Biosciences, Inc. | Compositions and methods for protein production |
CN103484496A (zh) * | 2013-07-31 | 2014-01-01 | 新乡医学院 | 一种报告基因重组在染色体目的基因上的方法、位点及用途 |
US10006910B2 (en) | 2014-12-18 | 2018-06-26 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same |
WO2016100049A1 (en) | 2014-12-18 | 2016-06-23 | Edico Genome Corporation | Chemically-sensitive field effect transistor |
US9857328B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same |
US9859394B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
US9618474B2 (en) | 2014-12-18 | 2017-04-11 | Edico Genome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
US10020300B2 (en) | 2014-12-18 | 2018-07-10 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
WO2017201081A1 (en) | 2016-05-16 | 2017-11-23 | Agilome, Inc. | Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US568772A (en) * | 1896-10-06 | Albert s | ||
CA1293460C (en) | 1985-10-07 | 1991-12-24 | Brian Lee Sauer | Site-specific recombination of dna in yeast |
GB2187415A (en) | 1986-03-05 | 1987-09-09 | Deere & Co | Machine for forming cylindrical bales of crop |
JPS62265992A (ja) * | 1986-05-12 | 1987-11-18 | Ajinomoto Co Inc | ヒト細胞を用いる有用物質の製造方法 |
US4956288A (en) * | 1988-04-22 | 1990-09-11 | Biogen, Inc. | Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA |
FR2646438B1 (fr) | 1989-03-20 | 2007-11-02 | Pasteur Institut | Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration |
EP0747485B1 (en) * | 1989-11-06 | 1998-12-02 | Cell Genesys, Inc. | Production of proteins using homologous recombination |
US5272071A (en) * | 1989-12-22 | 1993-12-21 | Applied Research Systems Ars Holding N.V. | Method for the modification of the expression characteristics of an endogenous gene of a given cell line |
ES2151463T5 (es) * | 1989-12-22 | 2012-10-29 | Merck Serono Sa | Constructos de ADN para la activación y la modificación de la expresión de genes endógenos |
WO1992015694A1 (en) * | 1991-03-08 | 1992-09-17 | The Salk Institute For Biological Studies | Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor |
US6270989B1 (en) * | 1991-11-05 | 2001-08-07 | Transkaryotic Therapies, Inc. | Protein production and delivery |
EP0626999A4 (en) * | 1992-01-24 | 1997-04-09 | Life Technologies Inc | Modulation of enzyme activities in the -i(in vivo) cloning of dna. |
TW402639B (en) * | 1992-12-03 | 2000-08-21 | Transkaryotic Therapies Inc | Protein production and protein delivery |
US5527695A (en) * | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
DE69432901T2 (de) * | 1993-12-23 | 2004-05-27 | Merck & Co., Inc. | Expressionssystem von antikörpern bei homologischer rekombinierung in murinezellen |
US6130364A (en) * | 1995-03-29 | 2000-10-10 | Abgenix, Inc. | Production of antibodies using Cre-mediated site-specific recombination |
US5882914A (en) * | 1995-06-06 | 1999-03-16 | The Johns Hopkins University School Of Medicine | Nucleic acids encoding the hypoxia inducible factor-1 |
US5695977A (en) * | 1995-08-31 | 1997-12-09 | Genetic Information Research Institute | Site directed recombination |
AUPN903196A0 (en) * | 1996-03-29 | 1996-04-26 | Australian National University, The | Single-step excision means |
US6020144A (en) * | 1996-09-12 | 2000-02-01 | Symbiontics, Inc. | Sustained delivery device comprising a Leishmania protozoa and methods of making and using the same |
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1998
- 1998-11-19 AU AU93284/98A patent/AU757930B2/en not_active Expired
- 1998-11-30 CA CA2252970A patent/CA2252970C/en not_active Expired - Lifetime
- 1998-11-30 KR KR10-1998-0051930A patent/KR100367062B1/ko active IP Right Grant
- 1998-11-30 ZA ZA9810915A patent/ZA9810915B/xx unknown
- 1998-12-01 EP EP99112607A patent/EP0957165B1/de not_active Revoked
- 1998-12-01 EP EP98122807.5A patent/EP0919619B2/de not_active Expired - Lifetime
- 1998-12-01 ES ES99112607T patent/ES2284228T3/es not_active Expired - Lifetime
- 1998-12-01 BR BR9805682-4A patent/BR9805682A/pt not_active Application Discontinuation
- 1998-12-01 TR TR1998/02503A patent/TR199802503A3/tr unknown
- 1998-12-01 JP JP34223498A patent/JP4629813B2/ja not_active Expired - Fee Related
- 1998-12-01 US US09/203,500 patent/US7008764B1/en not_active Expired - Lifetime
- 1998-12-01 CN CNB2004100638729A patent/CN100519751C/zh not_active Expired - Lifetime
- 1998-12-01 DK DK04006966T patent/DK1464705T3/da active
- 1998-12-01 DE DE59813976T patent/DE59813976D1/de not_active Revoked
- 1998-12-01 PT PT98122807T patent/PT919619E/pt unknown
- 1998-12-01 DE DE59813413T patent/DE59813413D1/de not_active Expired - Lifetime
- 1998-12-01 EP EP04006966A patent/EP1464705B1/de not_active Expired - Lifetime
- 1998-12-01 DK DK98122807.5T patent/DK0919619T4/da active
- 1998-12-01 AT AT04006966T patent/ATE320491T1/de active
- 1998-12-01 DK DK99112607T patent/DK0957165T3/da active
- 1998-12-01 AT AT99112607T patent/ATE360067T1/de active
- 1998-12-01 CN CNB981271782A patent/CN1232644C/zh not_active Expired - Lifetime
- 1998-12-01 DE DE59813438T patent/DE59813438D1/de not_active Expired - Lifetime
- 1998-12-01 AR ARP980106075A patent/AR018022A1/es active IP Right Grant
- 1998-12-01 PT PT99112607T patent/PT957165E/pt unknown
- 1998-12-01 ES ES98122807T patent/ES2258809T5/es not_active Expired - Lifetime
- 1998-12-01 ES ES04006966T patent/ES2259422T3/es not_active Expired - Lifetime
- 1998-12-01 PT PT04006966T patent/PT1464705E/pt unknown
- 1998-12-01 AT AT98122807T patent/ATE318904T1/de active
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1999
- 1999-10-08 AR ARP990105096A patent/AR020892A2/es active IP Right Grant
- 1999-10-08 AR ARP990105097A patent/AR020893A2/es active IP Right Grant
- 1999-10-08 AR ARP990105095A patent/AR020750A2/es active IP Right Grant
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Legal Events
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TC | Change of name | ||
HHT | Entry of change of name and/or headquarter and transfer of application, patent and certificate of addition of invention: republication |
Free format text: ALTERADO DE: BOEHRINGER MANNHEIM GMBH - REFERENCIA RPI NO. 1553 DE 10/10/2000 COD. 25.4 ITEM 71 |
|
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: DE ACORDO COM O ART. 37, INDEFIRO DO PRESENTE PEDIDO COM BASE NO DISPOSTO NO ART. 8O COMBINADO COMO ART. 13 DA LPI 9279 DE 14/05/96. |
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B12B | Appeal against refusal [chapter 12.2 patent gazette] |