ES2258809T5 - Optimización de células para la activación endógena del gen - Google Patents

Optimización de células para la activación endógena del gen

Info

Publication number
ES2258809T5
ES2258809T5 ES98122807T ES98122807T ES2258809T5 ES 2258809 T5 ES2258809 T5 ES 2258809T5 ES 98122807 T ES98122807 T ES 98122807T ES 98122807 T ES98122807 T ES 98122807T ES 2258809 T5 ES2258809 T5 ES 2258809T5
Authority
ES
Spain
Prior art keywords
expression
cell
nucleic acid
procedure
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98122807T
Other languages
English (en)
Other versions
ES2258809T3 (es
Inventor
Konrad Honold
Thomas Holtschke
Anne Stern
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8227717&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2258809(T5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of ES2258809T3 publication Critical patent/ES2258809T3/es
Publication of ES2258809T5 publication Critical patent/ES2258809T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

LA INVENCION TRATA DE PROCEDIMIENTOS PARA OPTIMIZACION DE LA EXPRESION GENICA EN CELULAS. UN PRIMER ASPECTO TRATA DE UN PROCEDIMIENTO PARA VARIAR LA EXPRESION DE UN GEN DIANA QUE SE ENCUENTRA DE MANERA ENDOGENA EN UNA CELULA EUCARIOTICA MEDIANTE LA INTERRUPCION DE UNA SECUENCIA HETEROLOGA DE CONTROL DE EXPRESION EN EL GENOMA DE LA CELULA MEDIANTE UNA RECOMBINACION HOMOLOGA, ASI COMO LA SEPARACION DEL DNA EXTRAÑO INSERTADO MEDIANTE UNA RECOMBINASA ESPECIFICA DE LUGAR Y SU SUSTITUCION MEDIANTE OTRAS SECUENCIAS HETEROLOGAS DE CONTROL DE EXPRESION Y/O GENES DE AMPLIFICACION. ADEMAS TRATA LA INVENCION DE LA INTRODUCCION DE UNA O VARIAS SECUENCIAS DE ACIDO NUCLEICO, A LAS CUALES SE UNE UNA PROTEINA DE UN ACTIVADOR O UN COMPLEJO DE PROTEINA DE ACTIVADOR, P. E. UN FACTOR INDUCIBLE DE LA HIPOXIA (HIF), EN EL GENOMA DE UNA CELULA EUCARIOTICA MEDIANTE RECOMBINACION HOMOLOGA, PARA VARIAR LA EXPRESION DE UN GEN DIANA. ADEMAS TRATA LA INVENCION DE UN PROCEDIMIENTO PARA PROBAR LA INFLUENCIA POR EL LADO 5'' O POR EL LADO 3 '' DE FRAGMENTOS NO CODIFICANTES DE ACIDO NUCLEICO EN LA EXPRESION DE UN GEN DIANA MEDIANTE LA DETERMINACION DE LA EXPRESION DE UN GEN INFORMADOR. ADEMAS TRATA LA INVENCION DE UN PROCEDIMIENTO PARA LA PREPARACION DE UNA CELULA EUCARIOTICA DHFR - NEGATIVA Y QUE CONTIENE UNA SECUENCIA DIANA DE RECOMBINASA, ASI COMO LA EXPRESION DE UNA SECUENCIA DE ACIDO NUCLEICO INSERTO EN LA SECUENCIA DIANA DE RECOMBINASA.
ES98122807T 1997-12-01 1998-12-01 Optimización de células para la activación endógena del gen Expired - Lifetime ES2258809T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97121075 1997-12-01
EP97121075 1997-12-01

Publications (2)

Publication Number Publication Date
ES2258809T3 ES2258809T3 (es) 2006-09-01
ES2258809T5 true ES2258809T5 (es) 2013-09-05

Family

ID=8227717

Family Applications (3)

Application Number Title Priority Date Filing Date
ES04006966T Expired - Lifetime ES2259422T3 (es) 1997-12-01 1998-12-01 Optimizacion de celulas para la activacion endogena del gen.
ES98122807T Expired - Lifetime ES2258809T5 (es) 1997-12-01 1998-12-01 Optimización de células para la activación endógena del gen
ES99112607T Expired - Lifetime ES2284228T3 (es) 1997-12-01 1998-12-01 Optimizacion de celulas para la activacion endogena del gen.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES04006966T Expired - Lifetime ES2259422T3 (es) 1997-12-01 1998-12-01 Optimizacion de celulas para la activacion endogena del gen.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES99112607T Expired - Lifetime ES2284228T3 (es) 1997-12-01 1998-12-01 Optimizacion de celulas para la activacion endogena del gen.

Country Status (16)

Country Link
US (1) US7008764B1 (es)
EP (3) EP0957165B1 (es)
JP (1) JP4629813B2 (es)
KR (1) KR100367062B1 (es)
CN (2) CN1232644C (es)
AR (4) AR018022A1 (es)
AT (3) ATE360067T1 (es)
AU (1) AU757930B2 (es)
BR (1) BR9805682A (es)
CA (1) CA2252970C (es)
DE (3) DE59813976D1 (es)
DK (3) DK0919619T4 (es)
ES (3) ES2259422T3 (es)
PT (3) PT919619E (es)
TR (1) TR199802503A3 (es)
ZA (1) ZA9810915B (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6316253B1 (en) 1999-11-01 2001-11-13 Chiron Corporation Expression vectors, transfection systems, and method of use thereof
WO2001066717A2 (en) * 2000-03-03 2001-09-13 The University Of Utah Gene targeting method
DE10023887A1 (de) * 2000-05-17 2001-11-29 Axel Haverich Verfahren zur transienten Insertion genetischer Elemente
US7314712B2 (en) 2001-07-27 2008-01-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
DE60310202T2 (de) 2002-01-23 2007-11-22 The University Of Utah Research Foundation, Salt Lake City Zielgerichtete chromosomale mutagenese mit zinkfingernukleasen
CA2497913C (en) 2002-09-05 2014-06-03 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
CA2579677A1 (en) * 2004-09-16 2006-03-30 Sangamo Biosciences, Inc. Compositions and methods for protein production
CA2615532C (en) * 2005-07-26 2016-06-28 Sangamo Biosciences, Inc. Targeted integration and expression of exogenous nucleic acid sequences
CN103484496A (zh) * 2013-07-31 2014-01-01 新乡医学院 一种报告基因重组在染色体目的基因上的方法、位点及用途
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
WO2016100049A1 (en) 2014-12-18 2016-06-23 Edico Genome Corporation Chemically-sensitive field effect transistor
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US568772A (en) * 1896-10-06 Albert s
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
GB2187415A (en) 1986-03-05 1987-09-09 Deere & Co Machine for forming cylindrical bales of crop
JPS62265992A (ja) * 1986-05-12 1987-11-18 Ajinomoto Co Inc ヒト細胞を用いる有用物質の製造方法
US4956288A (en) 1988-04-22 1990-09-11 Biogen, Inc. Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
FR2646438B1 (fr) * 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
WO1991006666A1 (en) * 1989-11-06 1991-05-16 Cell Genesys, Inc. Production of proteins using homologous recombination
US5272071A (en) * 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
DK0779362T4 (da) * 1989-12-22 2012-07-30 Serono Lab DNA-konstruktioner til aktivering og modifikation af endogene geners ekspression
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
US6270989B1 (en) 1991-11-05 2001-08-07 Transkaryotic Therapies, Inc. Protein production and delivery
EP0626999A4 (en) * 1992-01-24 1997-04-09 Life Technologies Inc Modulation of enzyme activities in the -i(in vivo) cloning of dna.
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
JP3654446B2 (ja) * 1993-12-23 2005-06-02 メルク アンド カンパニー， インコーポレイテッド マウス細胞のための相同的組換え抗体発現系
US6130364A (en) * 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US5882914A (en) 1995-06-06 1999-03-16 The Johns Hopkins University School Of Medicine Nucleic acids encoding the hypoxia inducible factor-1
US5695977A (en) * 1995-08-31 1997-12-09 Genetic Information Research Institute Site directed recombination
AUPN903196A0 (en) * 1996-03-29 1996-04-26 Australian National University, The Single-step excision means
US6020144A (en) * 1996-09-12 2000-02-01 Symbiontics, Inc. Sustained delivery device comprising a Leishmania protozoa and methods of making and using the same

Also Published As

Publication number Publication date
AU757930B2 (en) 2003-03-13
AR020892A2 (es) 2002-06-05
PT1464705E (pt) 2006-08-31
EP0919619A2 (de) 1999-06-02
EP0919619B2 (de) 2013-06-19
KR100367062B1 (ko) 2004-05-31
DK1464705T3 (da) 2006-07-24
CN1240829A (zh) 2000-01-12
AR020893A2 (es) 2002-06-05
EP0957165B1 (de) 2007-04-18
TR199802503A2 (xx) 1999-06-21
US7008764B1 (en) 2006-03-07
PT919619E (pt) 2006-07-31
JP4629813B2 (ja) 2011-02-09
KR19990062655A (ko) 1999-07-26
PT957165E (pt) 2007-07-19
EP1464705A1 (de) 2004-10-06
DE59813976D1 (de) 2007-05-31
EP0919619A3 (de) 1999-07-21
ES2259422T3 (es) 2006-10-01
ATE360067T1 (de) 2007-05-15
CN100519751C (zh) 2009-07-29
EP1464705B1 (de) 2006-03-15
DE59813438D1 (de) 2006-05-11
EP0957165A3 (de) 2000-10-04
EP0919619B1 (de) 2006-03-01
ZA9810915B (en) 2000-05-30
CA2252970A1 (en) 1999-06-01
DE59813413D1 (de) 2006-04-27
JPH11225785A (ja) 1999-08-24
BR9805682A (pt) 2000-04-11
EP0957165A2 (de) 1999-11-17
CA2252970C (en) 2011-11-15
AR020750A2 (es) 2002-05-29
ATE318904T1 (de) 2006-03-15
DK0919619T4 (da) 2013-09-23
DK0957165T3 (da) 2007-07-30
ES2258809T3 (es) 2006-09-01
CN1590551A (zh) 2005-03-09
DK0919619T3 (da) 2006-07-10
AR018022A1 (es) 2001-10-31
ATE320491T1 (de) 2006-04-15
CN1232644C (zh) 2005-12-21
AU9328498A (en) 1999-06-17
TR199802503A3 (tr) 1999-06-21
ES2284228T3 (es) 2007-11-01

Similar Documents

Publication Publication Date Title
ES2258809T5 (es) Optimización de células para la activación endógena del gen
AU2017335883B2 (en) RNA-guided nucleic acid modifying enzymes and methods of use thereof
JP2023082141A (ja) ＣａｓＺ組成物及び使用方法
US11713471B2 (en) Class II, type V CRISPR systems
ATE440950T1 (de) Konstrukte und verfahren zur regulation der genexpression
EP3712272A1 (en) Method for modulating rna splicing by inducing base mutation at splice site or base substitution in polypyrimidine region
KR20230076820A (ko) 진핵생물 유전체 공학을 위한 합성 미니어처 crispr-cas(casmini) 시스템
TW202128994A (zh) 真核半合成生物
US11866746B2 (en) Compositions comprising a variant Cas12i4 polypeptide and uses thereof
EP1728859A4 (en) SEQUENCE CAPABLE OF ACCELERATING GENE EXPRESSION AT A MODERATELY LOW TEMPERATURE
US20230045187A1 (en) Compositions comprising a nuclease and uses thereof
US20240011004A1 (en) Compositions comprising a variant crispr nuclease polypeptide and uses thereof
Wallace et al. The reaction specificities of the pea and a cyanobacterial thylakoid processing peptidase are similar but not identical
US20240042029A1 (en) Delivery of molecules to cells using trogocytosis and engineered cells
WO2023086965A2 (en) Type vii nucleases
WO2023086938A2 (en) Type v nucleases
WO2023086973A1 (en) Type ii nucleases
WO2022187278A1 (en) Nucleic acid detection and analysis systems
CA3189662A1 (en) Compositions comprising a nuclease and uses thereof
WO2024020111A1 (en) Systems for cell programming and methods thereof
AR024421A1 (es) Gen involucrado en el silenciamiento de genes epigeneticos.