AR020750A2 - Un procedimiento para la modificacion de la expresion de una secuencia de acido nucleico presente endogenamente en una celula humana y una celula humana obtenida por dicho procedimiento que no puede dar lugar a un individuo completo. - Google Patents

Un procedimiento para la modificacion de la expresion de una secuencia de acido nucleico presente endogenamente en una celula humana y una celula humana obtenida por dicho procedimiento que no puede dar lugar a un individuo completo.

Info

Publication number
AR020750A2
AR020750A2 ARP990105095A ARP990105095A AR020750A2 AR 020750 A2 AR020750 A2 AR 020750A2 AR P990105095 A ARP990105095 A AR P990105095A AR P990105095 A ARP990105095 A AR P990105095A AR 020750 A2 AR020750 A2 AR 020750A2
Authority
AR
Argentina
Prior art keywords
procedure
human cell
nucleic acid
expression
cell
Prior art date
Application number
ARP990105095A
Other languages
English (en)
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8227717&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR020750(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of AR020750A2 publication Critical patent/AR020750A2/es

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un procedimiento para la modificacion de la expresion de una secuencia de ácido nucleico presente endogenamente en una célula eucariotica, procedimientoque comprende los pasos: a)la célula es transfectada con un vector que comprende (i)al menos unas ecuencia de ácido nucleico que liga una proteína activadora,(ii)un gen marcador de seleccion positiva, (iii)secuencia de ADN homologas a un sector de ácido nucleico en el genoma de la célula, que flanquean lassecuencias de ADN (i) y (ii), parapermit ir una recombinacion homologa, b)la célula transfectada es cultivada bajo condiciones bajo las cuales se produce unarecombinacion homologa del vector, y c)la célula obtenida segun la etapa (b) es recuperada. La invencion también se refiere aun vecto r a ser usado con elprocedimiento y a una célula eucariotica obtenida por el mismo.
ARP990105095A 1997-12-01 1999-10-08 Un procedimiento para la modificacion de la expresion de una secuencia de acido nucleico presente endogenamente en una celula humana y una celula humana obtenida por dicho procedimiento que no puede dar lugar a un individuo completo. AR020750A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97121075 1997-12-01

Publications (1)

Publication Number Publication Date
AR020750A2 true AR020750A2 (es) 2002-05-29

Family

ID=8227717

Family Applications (4)

Application Number Title Priority Date Filing Date
ARP980106075A AR018022A1 (es) 1997-12-01 1998-12-01 UN PROCEDIMIENTO PARA LA MODIFICACIoN DE LA EXPRESIoN DE UNA SECUENCIA DE ÁCIDO NUCLEICO PRESENTE ENDoGENAMENTE EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA LA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.
ARP990105097A AR020893A2 (es) 1997-12-01 1999-10-08 UN PROCEDIMIENTO PARA PROVEER UNA CÉLULA EUCARIoTICA DHFR NEGATIVA, UN PROCEDIMIENTO PARA INTRODUCIR DE UN GEN DHFR HETERoLOGO EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.
ARP990105095A AR020750A2 (es) 1997-12-01 1999-10-08 Un procedimiento para la modificacion de la expresion de una secuencia de acido nucleico presente endogenamente en una celula humana y una celula humana obtenida por dicho procedimiento que no puede dar lugar a un individuo completo.
ARP990105096A AR020892A2 (es) 1997-12-01 1999-10-08 Un procedimiento para ensayar la influencia de secuencias de acido nucleico no codificadores de la region de un gen diana presente endogenamente en una celula eucariotica sobre la expresion de dicho gen diana.

Family Applications Before (2)

Application Number Title Priority Date Filing Date
ARP980106075A AR018022A1 (es) 1997-12-01 1998-12-01 UN PROCEDIMIENTO PARA LA MODIFICACIoN DE LA EXPRESIoN DE UNA SECUENCIA DE ÁCIDO NUCLEICO PRESENTE ENDoGENAMENTE EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA LA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.
ARP990105097A AR020893A2 (es) 1997-12-01 1999-10-08 UN PROCEDIMIENTO PARA PROVEER UNA CÉLULA EUCARIoTICA DHFR NEGATIVA, UN PROCEDIMIENTO PARA INTRODUCIR DE UN GEN DHFR HETERoLOGO EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP990105096A AR020892A2 (es) 1997-12-01 1999-10-08 Un procedimiento para ensayar la influencia de secuencias de acido nucleico no codificadores de la region de un gen diana presente endogenamente en una celula eucariotica sobre la expresion de dicho gen diana.

Country Status (16)

Country Link
US (1) US7008764B1 (es)
EP (3) EP1464705B1 (es)
JP (1) JP4629813B2 (es)
KR (1) KR100367062B1 (es)
CN (2) CN1232644C (es)
AR (4) AR018022A1 (es)
AT (3) ATE318904T1 (es)
AU (1) AU757930B2 (es)
BR (1) BR9805682A (es)
CA (1) CA2252970C (es)
DE (3) DE59813413D1 (es)
DK (3) DK0919619T4 (es)
ES (3) ES2259422T3 (es)
PT (3) PT919619E (es)
TR (1) TR199802503A3 (es)
ZA (1) ZA9810915B (es)

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WO2001066717A2 (en) * 2000-03-03 2001-09-13 The University Of Utah Gene targeting method
DE10023887A1 (de) * 2000-05-17 2001-11-29 Axel Haverich Verfahren zur transienten Insertion genetischer Elemente
WO2003012036A2 (en) * 2001-07-27 2003-02-13 The Government Of The United States Of America As Represented By The Secretary Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
WO2003087341A2 (en) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
EP2806025B1 (en) 2002-09-05 2019-04-03 California Institute of Technology Use of zinc finger nucleases to stimulate gene targeting
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
CA2579677A1 (en) * 2004-09-16 2006-03-30 Sangamo Biosciences, Inc. Compositions and methods for protein production
CN101273141B (zh) * 2005-07-26 2013-03-27 桑格摩生物科学股份有限公司 外源核酸序列的靶向整合和表达
CN103484496A (zh) * 2013-07-31 2014-01-01 新乡医学院 一种报告基因重组在染色体目的基因上的方法、位点及用途
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US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
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US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
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Also Published As

Publication number Publication date
AR020892A2 (es) 2002-06-05
CN1240829A (zh) 2000-01-12
DK0957165T3 (da) 2007-07-30
EP0919619B1 (de) 2006-03-01
DE59813413D1 (de) 2006-04-27
US7008764B1 (en) 2006-03-07
CN1232644C (zh) 2005-12-21
KR100367062B1 (ko) 2004-05-31
JP4629813B2 (ja) 2011-02-09
CA2252970C (en) 2011-11-15
AU757930B2 (en) 2003-03-13
DK1464705T3 (da) 2006-07-24
CN100519751C (zh) 2009-07-29
ES2259422T3 (es) 2006-10-01
AU9328498A (en) 1999-06-17
TR199802503A2 (xx) 1999-06-21
EP0957165A2 (de) 1999-11-17
ES2258809T3 (es) 2006-09-01
EP1464705B1 (de) 2006-03-15
JPH11225785A (ja) 1999-08-24
ES2284228T3 (es) 2007-11-01
ATE360067T1 (de) 2007-05-15
EP0919619A3 (de) 1999-07-21
EP0957165A3 (de) 2000-10-04
DK0919619T3 (da) 2006-07-10
EP1464705A1 (de) 2004-10-06
DE59813976D1 (de) 2007-05-31
BR9805682A (pt) 2000-04-11
EP0919619A2 (de) 1999-06-02
ATE320491T1 (de) 2006-04-15
DE59813438D1 (de) 2006-05-11
AR020893A2 (es) 2002-06-05
EP0957165B1 (de) 2007-04-18
PT919619E (pt) 2006-07-31
KR19990062655A (ko) 1999-07-26
DK0919619T4 (da) 2013-09-23
ZA9810915B (en) 2000-05-30
CN1590551A (zh) 2005-03-09
EP0919619B2 (de) 2013-06-19
CA2252970A1 (en) 1999-06-01
ATE318904T1 (de) 2006-03-15
PT1464705E (pt) 2006-08-31
TR199802503A3 (tr) 1999-06-21
AR018022A1 (es) 2001-10-31
PT957165E (pt) 2007-07-19
ES2258809T5 (es) 2013-09-05

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