ES2116683T3 - Gen de la beta-galactosidasa hipertermoestable. - Google Patents

Gen de la beta-galactosidasa hipertermoestable.

Info

Publication number
ES2116683T3
ES2116683T3 ES95303772T ES95303772T ES2116683T3 ES 2116683 T3 ES2116683 T3 ES 2116683T3 ES 95303772 T ES95303772 T ES 95303772T ES 95303772 T ES95303772 T ES 95303772T ES 2116683 T3 ES2116683 T3 ES 2116683T3
Authority
ES
Spain
Prior art keywords
hyperthermostable
galactosidase
gene
alpha
beta
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95303772T
Other languages
English (en)
Inventor
Atsushi Shimada
Nobuto Koyama
Kiyozo Asada
Miki Odate
Kimikazu Hashino
Ikunoshin Kato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takara Shuzo Co Ltd
Original Assignee
Takara Shuzo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Shuzo Co Ltd filed Critical Takara Shuzo Co Ltd
Application granted granted Critical
Publication of ES2116683T3 publication Critical patent/ES2116683T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase

Abstract

UN GEN ALFA-GALACTOSIDASA HIPERTERMOSTABLE RESISTENTE AL SDS AISLADO DERIVADO DE PYROCOCCUS FURIOSUS DE SECUENCIA SEQ ID NO: 2 Y GENES HIBRIZABLES CON EL MISMO. UN METODO PARA CLONAR EL GEN ALFA-GALACTOSIDASA HIPERTERMOSTABLE EN EL CUAL UNO DE LOS GENES ANTERIORES O PARTES DE LOS MISMOS, SE UTILIZAN COMO PRUEBA O INICIADOR. UN PROCESO PARA PRODUCIR UNA ALFA-GALACTOSIDASA HIPERTERMOSTABLE MEDIANTE EL CULTIVO DE UN TRANSFORMANTE EN EL CUAL SE HA INTRODUCIDO UN PLASMIDO QUE CONTIENE UNO DE LOS GENES ANTERIORES.
ES95303772T 1994-06-14 1995-06-02 Gen de la beta-galactosidasa hipertermoestable. Expired - Lifetime ES2116683T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15435694A JP3212798B2 (ja) 1994-06-14 1994-06-14 超耐熱性β−ガラクトシダーゼ遺伝子

Publications (1)

Publication Number Publication Date
ES2116683T3 true ES2116683T3 (es) 1998-07-16

Family

ID=15582374

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95303772T Expired - Lifetime ES2116683T3 (es) 1994-06-14 1995-06-02 Gen de la beta-galactosidasa hipertermoestable.

Country Status (5)

Country Link
EP (1) EP0687732B1 (es)
JP (1) JP3212798B2 (es)
CN (1) CN1056648C (es)
DE (1) DE69502343T2 (es)
ES (1) ES2116683T3 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6004788A (en) 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
AU767618C (en) * 1995-07-18 2004-09-09 Bp Corporation North America Inc. Screening methods for enzymes and enzyme kits
AU726017C (en) * 1996-01-11 2006-09-14 Bp Corporation North America Inc. Glycosidase enzymes
EP1469066B1 (en) * 1996-01-11 2008-04-09 Verenium Corporation Endoglucanase from Thermotoga
US5958751A (en) * 1996-03-08 1999-09-28 Diversa Corporation α-galactosidase
US6987016B2 (en) 1996-03-08 2006-01-17 Diversa Corporation α-galactosidases and methods for making and using them
US5789228A (en) 1996-05-22 1998-08-04 Diversa Corporation Endoglucanases
US7465571B1 (en) 1996-05-22 2008-12-16 Verenium Corporation Endoglucanases
WO1998024799A1 (en) 1996-12-06 1998-06-11 Diversa Corporation Glycosidase enzymes
ATA37099A (de) * 1999-03-04 2000-02-15 Lactoprot Alpenlaendische Milc Verfahren zur enzymatischen lactosespaltung, insbesondere mittels membrandiffusionsreaktoren
CN100370029C (zh) * 2005-10-11 2008-02-20 山东大学 一种高效转糖基β-半乳糖苷酶基因
CN100398655C (zh) * 2005-10-11 2008-07-02 山东大学 一种转糖基α-半乳糖苷酶基因
JP2009273379A (ja) * 2008-05-13 2009-11-26 National Institute Of Advanced Industrial & Technology セルロースの糖化方法
CN101993864B (zh) * 2009-08-13 2012-12-26 中国农业大学 一种耐高温β-半乳糖苷酶及其编码基因与应用
CN102154239B (zh) * 2011-01-06 2012-12-12 中山大学 一种具有高效转糖基β-半乳糖苷酶的新基因及应用
CN102268421B (zh) * 2011-08-03 2012-12-12 山西恩泽生物技术有限公司 一种β-葡萄糖苷酶基因的克隆、表达及应用
CN111154740B (zh) * 2020-02-07 2022-03-04 中国农业大学 一种沙质微泡菌β-半乳糖苷酶及其编码基因与应用
CN114774394A (zh) * 2022-04-20 2022-07-22 南京工业大学 一种热稳定β半乳糖苷酶及其合成甘油半乳糖苷的应用
CN114958893B (zh) * 2022-06-14 2023-08-29 中农华威生物制药(湖北)有限公司 一种乳猪高温教槽料制备所需的乳糖酶的构建方法
CN117402858B (zh) * 2023-12-13 2024-03-15 北京科为博生物科技有限公司 一种耐热性提高的β-葡萄糖苷酶突变体

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6014774A (ja) * 1983-07-06 1985-01-25 日本電気株式会社 コネクタ
JP3086115B2 (ja) * 1993-01-08 2000-09-11 寳酒造株式会社 超耐熱性β−ガラクトシダーゼ遺伝子
DE69331261T2 (de) * 1992-10-05 2002-08-08 Takara Shuzo Co Hyperthermostabile beta-Galaktosidase

Also Published As

Publication number Publication date
CN1128799A (zh) 1996-08-14
EP0687732A1 (en) 1995-12-20
JP3212798B2 (ja) 2001-09-25
DE69502343T2 (de) 1999-02-04
EP0687732B1 (en) 1998-05-06
JPH07327685A (ja) 1995-12-19
DE69502343D1 (de) 1998-06-10
CN1056648C (zh) 2000-09-20

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