AR018022A1 - UN PROCEDIMIENTO PARA LA MODIFICACIoN DE LA EXPRESIoN DE UNA SECUENCIA DE ÁCIDO NUCLEICO PRESENTE ENDoGENAMENTE EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA LA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO. - Google Patents

UN PROCEDIMIENTO PARA LA MODIFICACIoN DE LA EXPRESIoN DE UNA SECUENCIA DE ÁCIDO NUCLEICO PRESENTE ENDoGENAMENTE EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA LA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.

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Publication number
AR018022A1
AR018022A1 ARP980106075A ARP980106075A AR018022A1 AR 018022 A1 AR018022 A1 AR 018022A1 AR P980106075 A ARP980106075 A AR P980106075A AR P980106075 A ARP980106075 A AR P980106075A AR 018022 A1 AR018022 A1 AR 018022A1
Authority
AR
Argentina
Prior art keywords
cell
vector
nucleic acid
eucariotic
procedure
Prior art date
Application number
ARP980106075A
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English (en)
Original Assignee
Roche Olivier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8227717&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR018022(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Roche Olivier filed Critical Roche Olivier
Publication of AR018022A1 publication Critical patent/AR018022A1/es

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un procedimiento para la modificacion de la expresion de una secuencia de ácido nucleico presente endogenamente en una célula eucariotica que comprende lospasos: (a) la célula es trasnfectada con un primer vector, que comprende :(i) al menos unasecu encia seleccionada de una primera secuencia de control deexpresion heterologa y un primer gen amplificador, (ii) un gen marcador de seleccion positiva (iii) al menos dos secuencias diana para una recombinasasitio-específica que flanquean encada caso las secuencias (i) y (ii), (iv) secuencias de ADN homologas a un sector de ácido nucleico en el genoma de la célulaque flanquean las secuencias (i), (ii), iii para permitir una recombinacion homologa. (b) la célula transfectada escultivada bajo con diciones bajo las cualesse produce una recombinacion homologa del vector, y (c) la célula obtenida segun la etapa (b) es recuperada. La invencion se refiere también a un vector paraser usado por dicho procedimiento, a un vectorpara la produccion del vector previamente mencionado y a una célula eucariotica que puede ser obtenida pordicho procedimiento.
ARP980106075A 1997-12-01 1998-12-01 UN PROCEDIMIENTO PARA LA MODIFICACIoN DE LA EXPRESIoN DE UNA SECUENCIA DE ÁCIDO NUCLEICO PRESENTE ENDoGENAMENTE EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA LA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO. AR018022A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97121075 1997-12-01

Publications (1)

Publication Number Publication Date
AR018022A1 true AR018022A1 (es) 2001-10-31

Family

ID=8227717

Family Applications (4)

Application Number Title Priority Date Filing Date
ARP980106075A AR018022A1 (es) 1997-12-01 1998-12-01 UN PROCEDIMIENTO PARA LA MODIFICACIoN DE LA EXPRESIoN DE UNA SECUENCIA DE ÁCIDO NUCLEICO PRESENTE ENDoGENAMENTE EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA LA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.
ARP990105097A AR020893A2 (es) 1997-12-01 1999-10-08 UN PROCEDIMIENTO PARA PROVEER UNA CÉLULA EUCARIoTICA DHFR NEGATIVA, UN PROCEDIMIENTO PARA INTRODUCIR DE UN GEN DHFR HETERoLOGO EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.
ARP990105095A AR020750A2 (es) 1997-12-01 1999-10-08 Un procedimiento para la modificacion de la expresion de una secuencia de acido nucleico presente endogenamente en una celula humana y una celula humana obtenida por dicho procedimiento que no puede dar lugar a un individuo completo.
ARP990105096A AR020892A2 (es) 1997-12-01 1999-10-08 Un procedimiento para ensayar la influencia de secuencias de acido nucleico no codificadores de la region de un gen diana presente endogenamente en una celula eucariotica sobre la expresion de dicho gen diana.

Family Applications After (3)

Application Number Title Priority Date Filing Date
ARP990105097A AR020893A2 (es) 1997-12-01 1999-10-08 UN PROCEDIMIENTO PARA PROVEER UNA CÉLULA EUCARIoTICA DHFR NEGATIVA, UN PROCEDIMIENTO PARA INTRODUCIR DE UN GEN DHFR HETERoLOGO EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.
ARP990105095A AR020750A2 (es) 1997-12-01 1999-10-08 Un procedimiento para la modificacion de la expresion de una secuencia de acido nucleico presente endogenamente en una celula humana y una celula humana obtenida por dicho procedimiento que no puede dar lugar a un individuo completo.
ARP990105096A AR020892A2 (es) 1997-12-01 1999-10-08 Un procedimiento para ensayar la influencia de secuencias de acido nucleico no codificadores de la region de un gen diana presente endogenamente en una celula eucariotica sobre la expresion de dicho gen diana.

Country Status (16)

Country Link
US (1) US7008764B1 (es)
EP (3) EP0957165B1 (es)
JP (1) JP4629813B2 (es)
KR (1) KR100367062B1 (es)
CN (2) CN100519751C (es)
AR (4) AR018022A1 (es)
AT (3) ATE318904T1 (es)
AU (1) AU757930B2 (es)
BR (1) BR9805682A (es)
CA (1) CA2252970C (es)
DE (3) DE59813413D1 (es)
DK (3) DK0957165T3 (es)
ES (3) ES2284228T3 (es)
PT (3) PT957165E (es)
TR (1) TR199802503A2 (es)
ZA (1) ZA9810915B (es)

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US6316253B1 (en) 1999-11-01 2001-11-13 Chiron Corporation Expression vectors, transfection systems, and method of use thereof
WO2001066717A2 (en) * 2000-03-03 2001-09-13 The University Of Utah Gene targeting method
DE10023887A1 (de) * 2000-05-17 2001-11-29 Axel Haverich Verfahren zur transienten Insertion genetischer Elemente
CA2455013A1 (en) * 2001-07-27 2003-02-13 Francesca Storici Systems for in vivo site-directed mutagenesis using oligonucleotides
AU2003251286B2 (en) 2002-01-23 2007-08-16 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
CA2497913C (en) 2002-09-05 2014-06-03 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
CA2579677A1 (en) * 2004-09-16 2006-03-30 Sangamo Biosciences, Inc. Compositions and methods for protein production
WO2007014275A2 (en) * 2005-07-26 2007-02-01 Sangamo Biosciences, Inc. Targeted integration and expression of exogenous nucleic acid sequences
CN103484496A (zh) * 2013-07-31 2014-01-01 新乡医学院 一种报告基因重组在染色体目的基因上的方法、位点及用途
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
EP3235010A4 (en) 2014-12-18 2018-08-29 Agilome, Inc. Chemically-sensitive field effect transistor
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids

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Also Published As

Publication number Publication date
DE59813413D1 (de) 2006-04-27
ZA9810915B (en) 2000-05-30
DE59813976D1 (de) 2007-05-31
US7008764B1 (en) 2006-03-07
CN1590551A (zh) 2005-03-09
CA2252970C (en) 2011-11-15
EP0957165A3 (de) 2000-10-04
AU9328498A (en) 1999-06-17
AR020893A2 (es) 2002-06-05
EP0919619B2 (de) 2013-06-19
ES2259422T3 (es) 2006-10-01
ATE360067T1 (de) 2007-05-15
TR199802503A3 (tr) 1999-06-21
EP0957165A2 (de) 1999-11-17
ATE320491T1 (de) 2006-04-15
AU757930B2 (en) 2003-03-13
ES2258809T3 (es) 2006-09-01
ES2258809T5 (es) 2013-09-05
PT919619E (pt) 2006-07-31
AR020750A2 (es) 2002-05-29
DK0957165T3 (da) 2007-07-30
CN1240829A (zh) 2000-01-12
EP0919619A2 (de) 1999-06-02
PT1464705E (pt) 2006-08-31
KR100367062B1 (ko) 2004-05-31
EP0919619A3 (de) 1999-07-21
DK0919619T4 (da) 2013-09-23
DK1464705T3 (da) 2006-07-24
ES2284228T3 (es) 2007-11-01
CA2252970A1 (en) 1999-06-01
DK0919619T3 (da) 2006-07-10
JP4629813B2 (ja) 2011-02-09
CN100519751C (zh) 2009-07-29
KR19990062655A (ko) 1999-07-26
PT957165E (pt) 2007-07-19
TR199802503A2 (xx) 1999-06-21
DE59813438D1 (de) 2006-05-11
CN1232644C (zh) 2005-12-21
AR020892A2 (es) 2002-06-05
JPH11225785A (ja) 1999-08-24
EP0919619B1 (de) 2006-03-01
EP0957165B1 (de) 2007-04-18
EP1464705B1 (de) 2006-03-15
ATE318904T1 (de) 2006-03-15
EP1464705A1 (de) 2004-10-06
BR9805682A (pt) 2000-04-11

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