ES2003462A6 - Un metodo de obtencion de un alto nivel de expresion de una proteina. - Google Patents

Un metodo de obtencion de un alto nivel de expresion de una proteina.

Info

Publication number
ES2003462A6
ES2003462A6 ES8602898A ES8602898A ES2003462A6 ES 2003462 A6 ES2003462 A6 ES 2003462A6 ES 8602898 A ES8602898 A ES 8602898A ES 8602898 A ES8602898 A ES 8602898A ES 2003462 A6 ES2003462 A6 ES 2003462A6
Authority
ES
Spain
Prior art keywords
high level
level expression
multiply
exogenous dna
vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8602898A
Other languages
English (en)
Inventor
Randal J Kaufman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetics Institute LLC
Original Assignee
Genetics Institute LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Institute LLC filed Critical Genetics Institute LLC
Publication of ES2003462A6 publication Critical patent/ES2003462A6/es
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/243Colony Stimulating Factors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745Blood coagulation or fibrinolysis factors
    • C07K14/755Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/78Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

UN METODO DE OBTENCION DE UN ALTO NIVEL DE EXPRESION DE UNA PROTEINA. UN METODO DE OBTENCION DE UN ALTO NIVEL DE EXPRESION DE UNA PROTEINA, COMPRENDE CULTIVBAR CELULAS EUCARIOTICAS QUE CONTIENEN COPIAS MULTIPLES DE UN GEN HETEROLOGO QUE CODIFICA A LA CITADA PROTEINA Y COPIAS MULTIPLES DE POR LO MENOS DOS GENES MARCADORES HETEROLOGOS DIFERENTES, AMPLIFICABLES Y SELECCIONABLES. EL METODO TIENE SU PRINCIPAL APLICACION EN EL CAMPO DE LA INGENIERIA GENETICA Y ESPECIALMENTE EN LA TRANSFORMACION DE CELULAS EUCARIOTICAS CON VECTORES CON EL FIN DE PRODUCIR PROTEINAS O POLIPEPTIDOS HETEROLOGOS EN GRANDES CANTIDADES.
ES8602898A 1985-11-05 1986-11-04 Un metodo de obtencion de un alto nivel de expresion de una proteina. Expired ES2003462A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US79537985A 1985-11-05 1985-11-05

Publications (1)

Publication Number Publication Date
ES2003462A6 true ES2003462A6 (es) 1988-11-01

Family

ID=25165378

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8602898A Expired ES2003462A6 (es) 1985-11-05 1986-11-04 Un metodo de obtencion de un alto nivel de expresion de una proteina.

Country Status (5)

Country Link
EP (1) EP0247145B1 (es)
AT (1) ATE72837T1 (es)
DE (1) DE3684017D1 (es)
ES (1) ES2003462A6 (es)
WO (1) WO1987002707A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8597910B1 (en) 1985-04-11 2013-12-03 Children's Medical Center Corporation DNA encoding Von Willebrand Factor (VWF) and methods and cells for producing VFW, and VFW produced by the DNA, methods and cells
EP0218692A4 (en) * 1985-04-11 1988-03-22 Childrens Medical Center VON WILLEBRAND FACTOR.
DE3806617A1 (de) * 1988-03-02 1989-09-14 Behringwerke Ag Erzeugung hochexprimierender, rekombinanter, eukaryotischer zellen
EP0426744A1 (en) * 1988-07-29 1991-05-15 Zymogenetics, Inc. High efficiency translation of polycistronic messages in eucaryotic cells
US5585237A (en) * 1993-10-25 1996-12-17 Creative Biomolecules, Inc. Methods and compositions for high protein production from recombinant DNA
US5658763A (en) * 1993-10-25 1997-08-19 Creative Biomolecules, Inc. Methods and compositions for high protein production from non-native DNA
AT403167B (de) * 1994-11-14 1997-11-25 Immuno Ag Selektion und expression von fremdproteinen mittels eines selektions-amplifikations-systems
EP1196566B1 (en) * 1999-07-12 2006-02-01 Genentech, Inc. Expression vectors and methods

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399216A (en) * 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4396601A (en) * 1980-03-26 1983-08-02 The Regents Of The University Of Calif. Gene transfer in intact mammals
DE3382068D1 (de) * 1982-03-15 1991-01-31 Univ Columbia Verfahren zum einbringen von klonierten amplifizierbaren genen in eukaryotische zellen und zur herstellung von proteinprodukten.
EP0221955A4 (en) * 1985-05-01 1988-12-22 Genetics Inst HIGH LEVEL REINFORCEMENT AND EXPRESSION OF EXOGENER DNA.

Also Published As

Publication number Publication date
EP0247145B1 (en) 1992-02-26
ATE72837T1 (de) 1992-03-15
DE3684017D1 (de) 1992-04-02
EP0247145A4 (en) 1988-11-23
WO1987002707A1 (en) 1987-05-07
EP0247145A1 (en) 1987-12-02

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