ES2003462A6 - Un metodo de obtencion de un alto nivel de expresion de una proteina. - Google Patents
Un metodo de obtencion de un alto nivel de expresion de una proteina.Info
- Publication number
- ES2003462A6 ES2003462A6 ES8602898A ES8602898A ES2003462A6 ES 2003462 A6 ES2003462 A6 ES 2003462A6 ES 8602898 A ES8602898 A ES 8602898A ES 8602898 A ES8602898 A ES 8602898A ES 2003462 A6 ES2003462 A6 ES 2003462A6
- Authority
- ES
- Spain
- Prior art keywords
- high level
- level expression
- multiply
- exogenous dna
- vectors
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/243—Colony Stimulating Factors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
UN METODO DE OBTENCION DE UN ALTO NIVEL DE EXPRESION DE UNA PROTEINA. UN METODO DE OBTENCION DE UN ALTO NIVEL DE EXPRESION DE UNA PROTEINA, COMPRENDE CULTIVBAR CELULAS EUCARIOTICAS QUE CONTIENEN COPIAS MULTIPLES DE UN GEN HETEROLOGO QUE CODIFICA A LA CITADA PROTEINA Y COPIAS MULTIPLES DE POR LO MENOS DOS GENES MARCADORES HETEROLOGOS DIFERENTES, AMPLIFICABLES Y SELECCIONABLES. EL METODO TIENE SU PRINCIPAL APLICACION EN EL CAMPO DE LA INGENIERIA GENETICA Y ESPECIALMENTE EN LA TRANSFORMACION DE CELULAS EUCARIOTICAS CON VECTORES CON EL FIN DE PRODUCIR PROTEINAS O POLIPEPTIDOS HETEROLOGOS EN GRANDES CANTIDADES.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US79537985A | 1985-11-05 | 1985-11-05 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2003462A6 true ES2003462A6 (es) | 1988-11-01 |
Family
ID=25165378
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES8602898A Expired ES2003462A6 (es) | 1985-11-05 | 1986-11-04 | Un metodo de obtencion de un alto nivel de expresion de una proteina. |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0247145B1 (es) |
AT (1) | ATE72837T1 (es) |
DE (1) | DE3684017D1 (es) |
ES (1) | ES2003462A6 (es) |
WO (1) | WO1987002707A1 (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8597910B1 (en) | 1985-04-11 | 2013-12-03 | Children's Medical Center Corporation | DNA encoding Von Willebrand Factor (VWF) and methods and cells for producing VFW, and VFW produced by the DNA, methods and cells |
EP0218692A4 (en) * | 1985-04-11 | 1988-03-22 | Childrens Medical Center | VON WILLEBRAND FACTOR. |
DE3806617A1 (de) * | 1988-03-02 | 1989-09-14 | Behringwerke Ag | Erzeugung hochexprimierender, rekombinanter, eukaryotischer zellen |
EP0426744A1 (en) * | 1988-07-29 | 1991-05-15 | Zymogenetics, Inc. | High efficiency translation of polycistronic messages in eucaryotic cells |
US5585237A (en) * | 1993-10-25 | 1996-12-17 | Creative Biomolecules, Inc. | Methods and compositions for high protein production from recombinant DNA |
US5658763A (en) * | 1993-10-25 | 1997-08-19 | Creative Biomolecules, Inc. | Methods and compositions for high protein production from non-native DNA |
AT403167B (de) * | 1994-11-14 | 1997-11-25 | Immuno Ag | Selektion und expression von fremdproteinen mittels eines selektions-amplifikations-systems |
EP1196566B1 (en) * | 1999-07-12 | 2006-02-01 | Genentech, Inc. | Expression vectors and methods |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4399216A (en) * | 1980-02-25 | 1983-08-16 | The Trustees Of Columbia University | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
US4396601A (en) * | 1980-03-26 | 1983-08-02 | The Regents Of The University Of Calif. | Gene transfer in intact mammals |
DE3382068D1 (de) * | 1982-03-15 | 1991-01-31 | Univ Columbia | Verfahren zum einbringen von klonierten amplifizierbaren genen in eukaryotische zellen und zur herstellung von proteinprodukten. |
EP0221955A4 (en) * | 1985-05-01 | 1988-12-22 | Genetics Inst | HIGH LEVEL REINFORCEMENT AND EXPRESSION OF EXOGENER DNA. |
-
1986
- 1986-10-28 DE DE8686907081T patent/DE3684017D1/de not_active Expired - Lifetime
- 1986-10-28 AT AT86907081T patent/ATE72837T1/de not_active IP Right Cessation
- 1986-10-28 WO PCT/US1986/002326 patent/WO1987002707A1/en active IP Right Grant
- 1986-10-28 EP EP86907081A patent/EP0247145B1/en not_active Expired - Lifetime
- 1986-11-04 ES ES8602898A patent/ES2003462A6/es not_active Expired
Also Published As
Publication number | Publication date |
---|---|
EP0247145B1 (en) | 1992-02-26 |
ATE72837T1 (de) | 1992-03-15 |
DE3684017D1 (de) | 1992-04-02 |
EP0247145A4 (en) | 1988-11-23 |
WO1987002707A1 (en) | 1987-05-07 |
EP0247145A1 (en) | 1987-12-02 |
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