MX9606476A - Inhibicion citoplasmica de expresion de genes. - Google Patents

Inhibicion citoplasmica de expresion de genes.

Info

Publication number
MX9606476A
MX9606476A MX9606476A MX9606476A MX9606476A MX 9606476 A MX9606476 A MX 9606476A MX 9606476 A MX9606476 A MX 9606476A MX 9606476 A MX9606476 A MX 9606476A MX 9606476 A MX9606476 A MX 9606476A
Authority
MX
Mexico
Prior art keywords
cells
rna
plant
expression
interest
Prior art date
Application number
MX9606476A
Other languages
English (en)
Inventor
Monto H Kumagai
Guy R Della-Cioppa
Jonathan Donson
Damon A Harvey
Original Assignee
Biosource Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biosource Tech Inc filed Critical Biosource Tech Inc
Publication of MX9606476A publication Critical patent/MX9606476A/es

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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Abstract

Un aspecto de la invencion es proporcionar construcciones genéticas novedosas para la expresion de ARN inhibitorio en el citoplasma de células eucarioticas. El ARN inhibitorio puede ser un ARN de anti sentido o bien un ARN co-supresor. Las construcciones genéticas de la invencion pueden replicarse en el citoplasma de una célula eucariotica y comprenden una region promotora en combinacion funcional con un polinucleotido de codificacion. Las construcciones genéticas pueden ser diseñadas para replicarse en el citoplasma de células vegetales, células de levadura, y células de mamíferos. Cuando la célula eucariotica de interés es una célula vegetal, la construccion genética se deriva de preferencia de un virus de ARN vegetal. El virus de ARN vegetal derivado de construcciones genéticas puede emplear un promotor subgenomico de virus de planta, incluyendo promotores subgenomicos de tobamovirus en combinacion funcional con la region de codificacion de ARN. Otro aspecto de la presente invencion es proporcionar células que comprenden las construcciones genéticas de la invencion y organismo que comprende una pluralidad de tales células. Otro aspecto de la presente invencion es proporcionar métodos para reducir la expresion de un gen de interés en células eucarioticas, es decir, métodos para producir células eucarioticas que presentan niveles reducidos de expresion de un gen de interés. Los métodos de la presente invencion comprenden el paso de transfectar una célula con una construccion genética de la invencion en donde la region que codifica el ARN es específica para el gen de interés. Otro aspecto de la presente invencion es proporcionar células vegetales que producen niveles elevados de fitoeno carotenoide. Los niveles elevados de fitoeno se logran mediante la inhibicion de la expresion en la enzima fitoeno desaturasa usando los vectores la invencion.
MX9606476A 1994-06-16 1995-05-26 Inhibicion citoplasmica de expresion de genes. MX9606476A (es)

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US08/260,546 US5922602A (en) 1988-02-26 1994-06-16 Cytoplasmic inhibition of gene expression
PCT/US1995/006741 WO1995034668A2 (en) 1994-06-16 1995-05-26 The cytoplasmic inhibition of gene expression

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