CA2309028A1 - The cytoplasmic inhibition of gene expression - Google Patents
The cytoplasmic inhibition of gene expression Download PDFInfo
- Publication number
- CA2309028A1 CA2309028A1 CA002309028A CA2309028A CA2309028A1 CA 2309028 A1 CA2309028 A1 CA 2309028A1 CA 002309028 A CA002309028 A CA 002309028A CA 2309028 A CA2309028 A CA 2309028A CA 2309028 A1 CA2309028 A1 CA 2309028A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- rna
- plant
- expression
- interest
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000014509 gene expression Effects 0.000 title abstract 5
- 230000001086 cytosolic effect Effects 0.000 title 1
- 230000005764 inhibitory process Effects 0.000 title 1
- 238000010276 construction Methods 0.000 abstract 7
- 210000004027 cell Anatomy 0.000 abstract 6
- 230000002068 genetic effect Effects 0.000 abstract 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 5
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 5
- YVLPJIGOMTXXLP-UHFFFAOYSA-N 15-cis-phytoene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CC=CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C YVLPJIGOMTXXLP-UHFFFAOYSA-N 0.000 abstract 4
- 210000000805 cytoplasm Anatomy 0.000 abstract 3
- 230000002401 inhibitory effect Effects 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- YVLPJIGOMTXXLP-UUKUAVTLSA-N 15,15'-cis-Phytoene Natural products C(=C\C=C/C=C(\CC/C=C(\CC/C=C(\CC/C=C(\C)/C)/C)/C)/C)(\CC/C=C(\CC/C=C(\CC/C=C(\C)/C)/C)/C)/C YVLPJIGOMTXXLP-UUKUAVTLSA-N 0.000 abstract 2
- YVLPJIGOMTXXLP-BAHRDPFUSA-N 15Z-phytoene Natural products CC(=CCCC(=CCCC(=CCCC(=CC=C/C=C(C)/CCC=C(/C)CCC=C(/C)CCC=C(C)C)C)C)C)C YVLPJIGOMTXXLP-BAHRDPFUSA-N 0.000 abstract 2
- 108091027544 Subgenomic mRNA Proteins 0.000 abstract 2
- 241001493065 dsRNA viruses Species 0.000 abstract 2
- 235000011765 phytoene Nutrition 0.000 abstract 2
- 108020005544 Antisense RNA Proteins 0.000 abstract 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 abstract 1
- 108020005089 Plant RNA Proteins 0.000 abstract 1
- 241000723848 Tobamovirus Species 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 235000021466 carotenoid Nutrition 0.000 abstract 1
- 150000001747 carotenoids Chemical class 0.000 abstract 1
- 239000003184 complementary RNA Substances 0.000 abstract 1
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 108010001545 phytoene dehydrogenase Proteins 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 230000003362 replicative effect Effects 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
- 210000005253 yeast cell Anatomy 0.000 abstract 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
One aspect of the invention is to provide novel genetic constructions for the expression of inhibitory RNA in the cytoplasm of eukaryotic cells. The inhibitory RNA may be an anti-sense RNA or a co-suppressor RNA. The genetic constructions of the invention are capable of replicating in the cytoplasm of a eukaryotic cell and comprise a promoter region in functional combination with an encoding polynucleotide.
The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells.
When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus.
Plant RNA virus derived generic constructions , may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. Another aspect of the invention is to provide cells comprising the genetic constructions of the invention and organism comprising a plurality of such cells. Another aspect of the invention is to provide methods of reducing the expression of a gene of interest in eukaryotic cells, i.e., methods of producing eukaryotic cells exhibiting reduced levels of expression of a gene of interest. The methods of the invention comprise the step of transfecting a cell with a genetic construction of the invention in which the RNA
encoding region is specific for the gene of interest. Another aspect of the invention is to provide plant cells that produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression at the enzyme phytoene desaturase using the vectors of the invention.
The genetic constructions may be designed so as to replicate in the cytoplasm of plant cells, yeast cells, and mammalian cells.
When the eukaryotic cell of interest is a plant cell, the genetic construction is preferably derived from a plant RNA virus.
Plant RNA virus derived generic constructions , may employ a plant virus subgenomic promoter, including subgenomic promoters from tobamoviruses in functional combination with the RNA encoding region. Another aspect of the invention is to provide cells comprising the genetic constructions of the invention and organism comprising a plurality of such cells. Another aspect of the invention is to provide methods of reducing the expression of a gene of interest in eukaryotic cells, i.e., methods of producing eukaryotic cells exhibiting reduced levels of expression of a gene of interest. The methods of the invention comprise the step of transfecting a cell with a genetic construction of the invention in which the RNA
encoding region is specific for the gene of interest. Another aspect of the invention is to provide plant cells that produce elevated levels of the carotenoid phytoene. The elevated levels of phytoene are achieved by inhibiting the expression at the enzyme phytoene desaturase using the vectors of the invention.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/260,546 | 1994-06-14 | ||
| US08/260,546 US5922602A (en) | 1988-02-26 | 1994-06-16 | Cytoplasmic inhibition of gene expression |
| CA002193094A CA2193094C (en) | 1994-06-16 | 1995-05-26 | The cytoplasmic inhibition of gene expression |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002193094A Division CA2193094C (en) | 1994-06-14 | 1995-05-26 | The cytoplasmic inhibition of gene expression |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2309028A1 true CA2309028A1 (en) | 1995-12-21 |
| CA2309028C CA2309028C (en) | 2006-01-17 |
Family
ID=25678929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002309028A Expired - Fee Related CA2309028C (en) | 1994-06-14 | 1995-05-26 | The cytoplasmic inhibition of gene expression |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2309028C (en) |
-
1995
- 1995-05-26 CA CA002309028A patent/CA2309028C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2309028C (en) | 2006-01-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |