ES8401132A1 - Metodo de expresion de un gen codificador de un polipeptido util en una coagulacion de la leche. - Google Patents
Metodo de expresion de un gen codificador de un polipeptido util en una coagulacion de la leche.Info
- Publication number
- ES8401132A1 ES8401132A1 ES508813A ES508813A ES8401132A1 ES 8401132 A1 ES8401132 A1 ES 8401132A1 ES 508813 A ES508813 A ES 508813A ES 508813 A ES508813 A ES 508813A ES 8401132 A1 ES8401132 A1 ES 8401132A1
- Authority
- ES
- Spain
- Prior art keywords
- recombinant dna
- prorennin
- rennin
- dna means
- derived
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
METODO DE EXPRESION DE UN GEN CODIFICADOR DE UN POLIPEPTIDO PARA LA COAGULACION DE LA LECHE, QUE TIENE APLICACION PARA LA FABRICACION DE QUESO.CONSISTE EN SELECCIONAR UN GEN CON UN EXTREMO 5, TAL COMO UN GEN DE RENINA; Y UNA SECUENCIA DE NUCLEOTIDOS PROMOTORES DEL GEN QUE SE UNE AL EXTREMO 5 DEL GEN PARA FORMAR UN ADN RECOMBINANTE; INTRODUCIR ESTE EN UNA CELULA Y OBTENER UN ENZIMA PROPIO DE LA CELULA QUE TIENE ACTIVIDAD COAGULADORA DE LA LECHE. LA CELULA PUEDE SER UNA DE LEVADURA O BACTERIANA.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22571781A | 1981-01-16 | 1981-01-16 | |
US06/325,481 US4666847A (en) | 1981-01-16 | 1981-12-01 | Recombinant DNA means and method |
Publications (2)
Publication Number | Publication Date |
---|---|
ES508813A0 ES508813A0 (es) | 1983-12-01 |
ES8401132A1 true ES8401132A1 (es) | 1983-12-01 |
Family
ID=26919852
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES508813A Expired ES8401132A1 (es) | 1981-01-16 | 1982-01-16 | Metodo de expresion de un gen codificador de un polipeptido util en una coagulacion de la leche. |
ES524986A Expired ES8700320A1 (es) | 1981-01-16 | 1983-08-16 | Metodo de obtencion del exprimido de una materia seleccionada que contiene renina. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES524986A Expired ES8700320A1 (es) | 1981-01-16 | 1983-08-16 | Metodo de obtencion del exprimido de una materia seleccionada que contiene renina. |
Country Status (15)
Country | Link |
---|---|
US (1) | US4666847A (es) |
EP (1) | EP0057350B1 (es) |
JP (2) | JPH0716419B2 (es) |
AT (1) | ATE172493T1 (es) |
AU (1) | AU560340B2 (es) |
BR (1) | BR8200191A (es) |
CA (1) | CA1341300C (es) |
DE (1) | DE3280479T2 (es) |
DK (1) | DK15182A (es) |
ES (2) | ES8401132A1 (es) |
FI (1) | FI84080C (es) |
GB (1) | GB2091271B (es) |
IE (1) | IE52760B1 (es) |
NO (1) | NO820090L (es) |
NZ (1) | NZ199445A (es) |
Families Citing this family (51)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5525484A (en) * | 1981-01-16 | 1996-06-11 | Genome Therapeutics Corp. | Recombinant DNA means and method for producing rennin, prorenin and pre-prorennin |
NZ199722A (en) * | 1981-02-25 | 1985-12-13 | Genentech Inc | Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain |
IE53517B1 (en) * | 1981-06-17 | 1988-12-07 | Celltech Ltd | Process for the production of a polypeptide |
JPS5832896A (ja) * | 1981-08-24 | 1983-02-25 | Teruhiko Beppu | 複合プラスミド及びそれを含有する微生物 |
AU560472B2 (en) * | 1981-08-25 | 1987-04-09 | Celltech Limited | Yeast expression vectors |
BR8205954A (pt) * | 1981-10-14 | 1983-09-13 | Unilever Nv | Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos |
US4775622A (en) * | 1982-03-08 | 1988-10-04 | Genentech, Inc. | Expression, processing and secretion of heterologous protein by yeast |
ATE49421T1 (de) * | 1982-05-19 | 1990-01-15 | Gist Brocades Nv | Klonierungssystem fuer kluyveromyce spezies. |
IL68797A0 (en) * | 1982-07-01 | 1983-09-30 | Genex Corp | Cloned bovine calf chymosin and calf prochymosin genes,their preparation,plasmids containing them and microorganisms transformed by those plasmids |
US4961938A (en) * | 1982-12-22 | 1990-10-09 | Genentech, Inc. | Preparation of cheese with rennin from recombinant microbial cells |
IE56372B1 (en) * | 1982-12-22 | 1991-07-03 | Genentech Inc | Microbially produced rennet methods for its production and plasmids used for its production |
US4935369A (en) * | 1982-12-22 | 1990-06-19 | Genentech, Inc. | Isolation of active microbially produced bovine rennin |
US4661454A (en) * | 1983-02-28 | 1987-04-28 | Collaborative Research, Inc. | GAL1 yeast promoter linked to non galactokinase gene |
JPS59166086A (ja) * | 1983-03-09 | 1984-09-19 | Teruhiko Beppu | 新規な発現型プラスミドとそれらを用いて仔牛プロキモシン遺伝子を大腸菌内で発現させる方法 |
EP0129268B1 (en) * | 1983-05-19 | 1989-10-04 | Unilever N.V. | Improvements in the expression of newly introduced genes in yeast cells |
EP0128743A3 (en) * | 1983-06-07 | 1985-12-27 | Genex Corporation | Expression of foreign genes in yeast |
AU3036684A (en) * | 1983-07-07 | 1985-01-10 | Genex Corp. | Production of bovine calf chymosin |
US4935354A (en) * | 1983-07-14 | 1990-06-19 | Genentech, Inc. | Rennin from recombinant microbial cells for preparation of cheese |
US4721673A (en) * | 1983-09-01 | 1988-01-26 | Genex Corporation | Recovery and activation process for microbially produced calf prochymosin |
US5639639A (en) * | 1983-11-02 | 1997-06-17 | Genzyme Corporation | Recombinant heterodimeric human fertility hormones, and methods, cells, vectors and DNA for the production thereof |
US4935370A (en) * | 1983-12-23 | 1990-06-19 | Pfizer Inc. | Expression plasmids for improved production of heterologous protein in bacteria |
NL8400687A (nl) * | 1984-03-02 | 1985-10-01 | Unilever Nv | Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit. |
JPS60188077A (ja) * | 1984-03-09 | 1985-09-25 | Teruhiko Beppu | 仔牛プロキモシンcDΝAの全配列を有する新規な発現プラスミド |
JPS60188093A (ja) * | 1984-03-09 | 1985-09-25 | Teruhiko Beppu | 枯草菌に於ける形質発現プラスミド |
US5082775A (en) * | 1984-05-11 | 1992-01-21 | Berlex Laboratories, Inc. | Efficient process for isolating insoluble heterologous protein using non-ionic detergents |
ATE71147T1 (de) * | 1984-05-11 | 1992-01-15 | Berlex Lab | Verfahren zur herstellung eines im wesentlichen nichtloeslichen polypetids unter verwendung nichtionischer detergentien. |
GB2178431B (en) * | 1985-07-15 | 1989-11-22 | Bioteknika International | Genetically engineered yeast strains |
US5364770A (en) * | 1985-08-29 | 1994-11-15 | Genencor International Inc. | Heterologous polypeptides expressed in aspergillus |
US6004785A (en) * | 1985-08-29 | 1999-12-21 | Genencor International Inc. | Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same |
US4937189A (en) * | 1985-10-18 | 1990-06-26 | Pfizer Inc. | Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants |
US5766912A (en) * | 1986-03-17 | 1998-06-16 | Novo Nordisk A/S | Humicola lipase produced in aspergillus |
US5536661A (en) * | 1987-03-10 | 1996-07-16 | Novo Nordisk A/S | Process for the production of protein products in aspergillus |
JPH0673456B2 (ja) * | 1986-04-26 | 1994-09-21 | 三共株式会社 | ヒト・膵臓エラスタ−ゼ▲i▼ |
EP0254249B1 (en) * | 1986-07-22 | 1992-08-19 | Ciba-Geigy Ag | Preparation of binding factor related polypeptides |
GB2200118A (en) * | 1987-01-23 | 1988-07-27 | Allelix Inc | Synthetic chymosin-and prochymosin-encoding DNA segments |
US4977089A (en) * | 1987-01-30 | 1990-12-11 | Eli Lilly And Company | Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms |
US5162226A (en) | 1987-08-24 | 1992-11-10 | University Of Tennessee Research Corp. (U.T.R.C.) | Therapeutic compositions against streptococcal infections, transformed hosts, methods of immunization and genetically engineered products |
JPH02109984A (ja) * | 1988-12-01 | 1990-04-23 | Teruhiko Beppu | 複合プラスミド及びそれを含有する微生物 |
US5151358A (en) * | 1989-06-13 | 1992-09-29 | Genencor International, Inc. | Processes for the recovery of naturally produced chymosin |
US5139943A (en) * | 1989-06-13 | 1992-08-18 | Genencor International, Inc. | Processes for the recovery of microbially produced chymosin |
AU7493491A (en) * | 1990-03-13 | 1991-10-10 | Hawaii Biotechnology Group, Inc. | (neurospora) expression system |
IE914007A1 (en) * | 1990-11-19 | 1992-05-20 | Univ Illinois | A protein being genetically engineered to provide¹orientability and a substrate having an oriented layer of¹proteins |
EP1221488A1 (en) * | 1993-06-04 | 2002-07-10 | Whitehead Institute For Biomedical Research | Stress proteins and uses therefor |
US7157089B1 (en) * | 1996-11-26 | 2007-01-02 | Stressgen Biotechnologies Corporation | Immune responses using compositions containing stress proteins |
CA2378097A1 (en) * | 1999-07-08 | 2001-01-18 | Stressgen Biotechnologies Corporation | Induction of a th1-like response in vitro |
US7390936B1 (en) * | 1999-08-23 | 2008-06-24 | Sembiosys Genetics Inc. | Commercial production of chymosin in plants |
US6964851B2 (en) * | 1999-12-07 | 2005-11-15 | Stressgen Biotechnologies Corp. | Compositions and methods for detecting stress-inducible proteins |
EP1253939B1 (en) * | 2000-01-14 | 2009-08-05 | Whitehead Institute For Biomedical Research | In vivo ctl elicitation by heat shock protein fusion proteins maps to a discrete atp binding domain and is cd4+ t cell-independent |
CZ20024154A3 (cs) * | 2000-06-26 | 2003-08-13 | Stressgen Biotechnologies Corporation | Použití prostředku obsahujícího napojený protein pro výrobu farmaceutického přípravku pro léčení bradavic a chorob a stavů souvisejících s papiloma virem |
BR0206995A (pt) * | 2001-02-05 | 2005-08-16 | Stressgen Biotechnologies Corp | Tratamento do vìrus da hepatite b |
US7482148B2 (en) * | 2004-03-30 | 2009-01-27 | Sudershan Biotech Ltd. | Recombinant calf-chymosin and a process for producing the same |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4136201A (en) * | 1967-12-06 | 1979-01-23 | Gb Fermentation Industries, Inc. | Microbial rennin |
FR2441659A1 (fr) * | 1978-11-14 | 1980-06-13 | Anvar | Nouveaux plasmides hybrides et microorganismes les contenant |
US4332892A (en) * | 1979-01-15 | 1982-06-01 | President And Fellows Of Harvard College | Protein synthesis |
AU542264B2 (en) * | 1979-06-01 | 1985-02-14 | G.D. Searle & Co. | Plasmid vectors |
US4338397A (en) * | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
IE53517B1 (en) * | 1981-06-17 | 1988-12-07 | Celltech Ltd | Process for the production of a polypeptide |
-
1981
- 1981-12-01 US US06/325,481 patent/US4666847A/en not_active Expired - Lifetime
- 1981-12-18 CA CA000392669A patent/CA1341300C/en not_active Expired - Lifetime
-
1982
- 1982-01-08 AT AT82100124T patent/ATE172493T1/de not_active IP Right Cessation
- 1982-01-08 DE DE3280479T patent/DE3280479T2/de not_active Expired - Lifetime
- 1982-01-08 NZ NZ199445A patent/NZ199445A/en unknown
- 1982-01-08 EP EP82100124A patent/EP0057350B1/en not_active Expired - Lifetime
- 1982-01-11 FI FI820075A patent/FI84080C/fi not_active IP Right Cessation
- 1982-01-12 AU AU79456/82A patent/AU560340B2/en not_active Expired
- 1982-01-13 NO NO820090A patent/NO820090L/no unknown
- 1982-01-14 BR BR8200191A patent/BR8200191A/pt unknown
- 1982-01-14 JP JP57003556A patent/JPH0716419B2/ja not_active Expired - Lifetime
- 1982-01-15 GB GB8201120A patent/GB2091271B/en not_active Expired
- 1982-01-15 DK DK15182A patent/DK15182A/da not_active Application Discontinuation
- 1982-01-15 IE IE69/82A patent/IE52760B1/en not_active IP Right Cessation
- 1982-01-16 ES ES508813A patent/ES8401132A1/es not_active Expired
-
1983
- 1983-08-16 ES ES524986A patent/ES8700320A1/es not_active Expired
-
1994
- 1994-10-04 JP JP6264642A patent/JPH07108221B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0057350B1 (en) | 1998-10-21 |
AU560340B2 (en) | 1987-04-02 |
AU7945682A (en) | 1982-07-22 |
JPH07108221B2 (ja) | 1995-11-22 |
NZ199445A (en) | 1985-07-12 |
GB2091271B (en) | 1984-08-30 |
IE820069L (en) | 1983-06-01 |
FI84080C (fi) | 1991-10-10 |
CA1341300C (en) | 2001-10-09 |
EP0057350A3 (en) | 1982-12-01 |
ES8700320A1 (es) | 1986-10-01 |
ES524986A0 (es) | 1986-10-01 |
JPS57141287A (en) | 1982-09-01 |
NO820090L (no) | 1982-07-19 |
GB2091271A (en) | 1982-07-28 |
DE3280479T2 (de) | 1999-03-11 |
ATE172493T1 (de) | 1998-11-15 |
IE52760B1 (en) | 1988-02-17 |
US4666847A (en) | 1987-05-19 |
FI820075L (fi) | 1982-07-17 |
DK15182A (da) | 1982-07-17 |
JPH0716419B2 (ja) | 1995-03-01 |
BR8200191A (pt) | 1982-11-09 |
JPH07170978A (ja) | 1995-07-11 |
EP0057350A2 (en) | 1982-08-11 |
DE3280479D1 (de) | 1998-11-26 |
ES508813A0 (es) | 1983-12-01 |
FI84080B (fi) | 1991-06-28 |
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