ATE244311T1 - Expressionssystem von antikörpern bei homologischer rekombinierung in murinezellen - Google Patents

Expressionssystem von antikörpern bei homologischer rekombinierung in murinezellen

Info

Publication number
ATE244311T1
ATE244311T1 AT95903890T AT95903890T ATE244311T1 AT E244311 T1 ATE244311 T1 AT E244311T1 AT 95903890 T AT95903890 T AT 95903890T AT 95903890 T AT95903890 T AT 95903890T AT E244311 T1 ATE244311 T1 AT E244311T1
Authority
AT
Austria
Prior art keywords
expression system
murine cells
antibodies during
recombinant gene
locus
Prior art date
Application number
AT95903890T
Other languages
English (en)
Inventor
Gregory Franklin Hollis
George E Mark
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Application granted granted Critical
Publication of ATE244311T1 publication Critical patent/ATE244311T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT95903890T 1993-12-23 1994-12-22 Expressionssystem von antikörpern bei homologischer rekombinierung in murinezellen ATE244311T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17380093A 1993-12-23 1993-12-23
PCT/IB1995/000014 WO1995017516A1 (en) 1993-12-23 1994-12-22 Homologous recombination antibody expression system for murine cells

Publications (1)

Publication Number Publication Date
ATE244311T1 true ATE244311T1 (de) 2003-07-15

Family

ID=22633548

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95903890T ATE244311T1 (de) 1993-12-23 1994-12-22 Expressionssystem von antikörpern bei homologischer rekombinierung in murinezellen

Country Status (14)

Country Link
US (2) US6743622B2 (de)
EP (1) EP0731845B1 (de)
JP (1) JP3654446B2 (de)
AT (1) ATE244311T1 (de)
AU (1) AU691857B2 (de)
CA (1) CA2177959C (de)
CZ (1) CZ289308B6 (de)
DE (1) DE69432901T2 (de)
DK (1) DK0731845T3 (de)
ES (1) ES2202351T3 (de)
FI (1) FI119698B (de)
GB (1) GB2299336A (de)
HU (1) HU221513B (de)
WO (1) WO1995017516A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE244311T1 (de) * 1993-12-23 2003-07-15 Merck & Co Inc Expressionssystem von antikörpern bei homologischer rekombinierung in murinezellen
US5990091A (en) * 1997-03-12 1999-11-23 Virogenetics Corporation Vectors having enhanced expression, and methods of making and uses thereof
AU757930B2 (en) * 1997-12-01 2003-03-13 Roche Diagnostics Gmbh Optimization of cells for endogenous gene activation
US6521419B1 (en) 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US6800457B2 (en) 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
WO2002012437A2 (en) 2000-08-03 2002-02-14 Schooten Wim Van Production of humanized antibodies in transgenic animals
CA2421680A1 (en) 2000-09-29 2002-04-04 Merck & Co., Inc. Antibodies for human cytochrome p450 2d6
CN1296485C (zh) * 2002-01-17 2007-01-24 英国龙沙生物医药股份有限公司 能够产生蛋白质及能够在无谷氨酰胺的培养基上生长的谷氨酰胺营养缺陷型人类细胞
KR100976098B1 (ko) * 2002-07-18 2010-08-16 론자 바이올로직스 피엘씨 Ｃｈｏ 세포에서 재조합 단백질의 발현 방법
GB0216648D0 (en) * 2002-07-18 2002-08-28 Lonza Biologics Plc Method of expressing recombinant protein in CHO cells
AU2006206577B2 (en) 2005-01-21 2012-03-08 Merck Sharp & Dohme Corp. Polypeptides for inducing a protective immune response against Staphylococcus aureus
SG174053A1 (en) 2006-09-01 2011-09-29 Therapeutic Human Polyclonals Inc Enhanced expression of human or humanized immunoglobulin in non-human transgenic animals
AU2007319604B2 (en) 2006-10-19 2011-03-24 Csl Limited High affinity antibody antagonists of interleukin-13 receptor alpha 1
JP4998814B2 (ja) 2009-10-01 2012-08-15 Ｔｏｔｏ株式会社 Ｄｎａ構築物およびそれを用いた組み換えｃｈｏ細胞の製造方法
WO2011086001A1 (en) 2010-01-15 2011-07-21 Cormus Srl Antibodies for the treatment of hcv
US8747858B2 (en) 2010-07-13 2014-06-10 Merck Sharp & Dohme Corp. Staphylococcus aureus surface protein SA1789 and protective vaccine based thereon
EP2638058B1 (de) 2010-11-12 2017-01-11 Merck Sharp & Dohme Corp. Enolase-peptid-konjugat-impfstoffe gegen staphylococcus aureus
JP5209693B2 (ja) * 2010-12-10 2013-06-12 ロンザ・バイオロジクス・ピーエルシー Ｃｈｏ細胞において組換えタンパク質を発現する方法
EP2829608A1 (de) 2013-07-23 2015-01-28 Universität Bielefeld Verfahren zur Herstellung von rekombinanten Proteinen in Säugerzellen
WO2020084034A1 (en) 2018-10-26 2020-04-30 F. Hoffmann-La Roche Ag Multispecific antibody screening method using recombinase mediated cassette exchange

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5202238A (en) * 1987-10-27 1993-04-13 Oncogen Production of chimeric antibodies by homologous recombination
US5204244A (en) 1987-10-27 1993-04-20 Oncogen Production of chimeric antibodies by homologous recombination
HK1000547A1 (en) * 1989-12-22 1998-04-03 Merck Serono Sa Endogenous gene expression modification with regulatory element by way of homologous recombination
BR9107210A (pt) * 1990-12-07 1994-02-08 Univ Florida Celula hospedeira recombinante,cepa de celula,e processo para produzir cepa de celula
WO1993004169A1 (en) * 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
DE69333955D1 (de) * 1992-04-24 2006-02-02 Stanford Res Inst Int Targeting homologer sequenzen in eukaryotenzellen
AU4401993A (en) * 1992-06-04 1993-12-30 Exemplar Corporation Insertion of heterologous dna outside of known chromosomal genes
AU5087193A (en) * 1992-08-21 1994-03-15 Regents Of The University Of California, The Composition and method for altering dna sequences by homologous recombination
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
ATE244311T1 (de) * 1993-12-23 2003-07-15 Merck & Co Inc Expressionssystem von antikörpern bei homologischer rekombinierung in murinezellen
US5830698A (en) * 1997-03-14 1998-11-03 Idec Pharmaceuticals Corporation Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same

Also Published As

Publication number Publication date
US20030082673A1 (en) 2003-05-01
CZ289308B6 (cs) 2001-12-12
FI962268L (fi) 1996-06-20
HUT74844A (en) 1997-02-28
DK0731845T3 (da) 2003-10-20
CA2177959C (en) 2007-02-13
AU1278695A (en) 1995-07-10
WO1995017516A1 (en) 1995-06-29
ES2202351T3 (es) 2004-04-01
DE69432901D1 (de) 2003-08-07
JPH09510865A (ja) 1997-11-04
US20030124648A1 (en) 2003-07-03
DE69432901T2 (de) 2004-05-27
US6750041B2 (en) 2004-06-15
US6743622B2 (en) 2004-06-01
EP0731845B1 (de) 2003-07-02
CA2177959A1 (en) 1995-06-29
AU691857B2 (en) 1998-05-28
JP3654446B2 (ja) 2005-06-02
GB9613135D0 (en) 1996-08-28
FI119698B (fi) 2009-02-13
HU221513B (en) 2002-10-28
CZ185996A3 (en) 1996-10-16
EP0731845A1 (de) 1996-09-18
GB2299336A (en) 1996-10-02
FI962268A0 (fi) 1996-05-30
HU9601467D0 (en) 1996-07-29

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