AR020893A2 - UN PROCEDIMIENTO PARA PROVEER UNA CÉLULA EUCARIoTICA DHFR NEGATIVA, UN PROCEDIMIENTO PARA INTRODUCIR DE UN GEN DHFR HETERoLOGO EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO. - Google Patents

UN PROCEDIMIENTO PARA PROVEER UNA CÉLULA EUCARIoTICA DHFR NEGATIVA, UN PROCEDIMIENTO PARA INTRODUCIR DE UN GEN DHFR HETERoLOGO EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.

Info

Publication number
AR020893A2
AR020893A2 ARP990105097A ARP990105097A AR020893A2 AR 020893 A2 AR020893 A2 AR 020893A2 AR P990105097 A ARP990105097 A AR P990105097A AR P990105097 A ARP990105097 A AR P990105097A AR 020893 A2 AR020893 A2 AR 020893A2
Authority
AR
Argentina
Prior art keywords
procedure
cell
dhfr
vector
negative
Prior art date
Application number
ARP990105097A
Other languages
English (en)
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8227717&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR020893(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of AR020893A2 publication Critical patent/AR020893A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un procedimiento para la puesta a disposicion de una célula eucariotica DHFR negativa, que comprende las etapas de: (a) la célula es transfectada con unprimer vector que comprende (i) al menos una secuencia diana para una recombinasa sitio-específica , (ii) secuencias de ADN homologas a una secuencia de ácidonucleico DHFR presente endogenamente en la célula que flanquean la secuencia (i), para permitir una recombinacion homologa, y (iii) en caso dado, un genmarcador de seleccionpositiva y, en ca so dado un gen marcador de seleccion negativa, (b) la célula transfectada es cultivada bajo condiciones las cualesproduce una recombinacion homologa del vector, y (c) la célula obtenida segun el paso (b) es recuperada. La presentetambién se refiere a un procedimiento parala introduccion de un gen DHFR heterologo en la célula obtenida por dicho procedimiento, a un vector para llevar a cabo dichos procedimientos y a una célulaeucariotica obtenida por dichos procedimientos.
ARP990105097A 1997-12-01 1999-10-08 UN PROCEDIMIENTO PARA PROVEER UNA CÉLULA EUCARIoTICA DHFR NEGATIVA, UN PROCEDIMIENTO PARA INTRODUCIR DE UN GEN DHFR HETERoLOGO EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO. AR020893A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97121075 1997-12-01

Publications (1)

Publication Number Publication Date
AR020893A2 true AR020893A2 (es) 2002-06-05

Family

ID=8227717

Family Applications (4)

Application Number Title Priority Date Filing Date
ARP980106075A AR018022A1 (es) 1997-12-01 1998-12-01 UN PROCEDIMIENTO PARA LA MODIFICACIoN DE LA EXPRESIoN DE UNA SECUENCIA DE ÁCIDO NUCLEICO PRESENTE ENDoGENAMENTE EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA LA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.
ARP990105097A AR020893A2 (es) 1997-12-01 1999-10-08 UN PROCEDIMIENTO PARA PROVEER UNA CÉLULA EUCARIoTICA DHFR NEGATIVA, UN PROCEDIMIENTO PARA INTRODUCIR DE UN GEN DHFR HETERoLOGO EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.
ARP990105095A AR020750A2 (es) 1997-12-01 1999-10-08 Un procedimiento para la modificacion de la expresion de una secuencia de acido nucleico presente endogenamente en una celula humana y una celula humana obtenida por dicho procedimiento que no puede dar lugar a un individuo completo.
ARP990105096A AR020892A2 (es) 1997-12-01 1999-10-08 Un procedimiento para ensayar la influencia de secuencias de acido nucleico no codificadores de la region de un gen diana presente endogenamente en una celula eucariotica sobre la expresion de dicho gen diana.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP980106075A AR018022A1 (es) 1997-12-01 1998-12-01 UN PROCEDIMIENTO PARA LA MODIFICACIoN DE LA EXPRESIoN DE UNA SECUENCIA DE ÁCIDO NUCLEICO PRESENTE ENDoGENAMENTE EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA LA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.

Family Applications After (2)

Application Number Title Priority Date Filing Date
ARP990105095A AR020750A2 (es) 1997-12-01 1999-10-08 Un procedimiento para la modificacion de la expresion de una secuencia de acido nucleico presente endogenamente en una celula humana y una celula humana obtenida por dicho procedimiento que no puede dar lugar a un individuo completo.
ARP990105096A AR020892A2 (es) 1997-12-01 1999-10-08 Un procedimiento para ensayar la influencia de secuencias de acido nucleico no codificadores de la region de un gen diana presente endogenamente en una celula eucariotica sobre la expresion de dicho gen diana.

Country Status (16)

Country Link
US (1) US7008764B1 (es)
EP (3) EP0957165B1 (es)
JP (1) JP4629813B2 (es)
KR (1) KR100367062B1 (es)
CN (2) CN1232644C (es)
AR (4) AR018022A1 (es)
AT (3) ATE318904T1 (es)
AU (1) AU757930B2 (es)
BR (1) BR9805682A (es)
CA (1) CA2252970C (es)
DE (3) DE59813413D1 (es)
DK (3) DK1464705T3 (es)
ES (3) ES2284228T3 (es)
PT (3) PT919619E (es)
TR (1) TR199802503A2 (es)
ZA (1) ZA9810915B (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2278022A3 (en) * 1999-11-01 2011-05-18 Novartis Vaccines and Diagnostics, Inc. Expression vectors, transfection systems, and method of use thereof
WO2001066717A2 (en) * 2000-03-03 2001-09-13 The University Of Utah Gene targeting method
DE10023887A1 (de) * 2000-05-17 2001-11-29 Axel Haverich Verfahren zur transienten Insertion genetischer Elemente
JP2005500841A (ja) * 2001-07-27 2005-01-13 アメリカ合衆国 オリゴヌクレオチドを用いるインビボ部位指定変異誘発のためのシステム
CN100575485C (zh) 2002-01-23 2009-12-30 犹他大学研究基金会 使用锌指核酸酶的定向染色体诱变
US9447434B2 (en) 2002-09-05 2016-09-20 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
KR20070060115A (ko) * 2004-09-16 2007-06-12 상가모 바이오사이언스 인코포레이티드 단백질 생산을 위한 조성물 및 방법
EP1913149A4 (en) * 2005-07-26 2009-08-05 Sangamo Biosciences Inc TARGETED INTEGRATION AND EXPRESSION OF EXOGENOUS NUCLEIC ACID SEQUENCES
CN103484496A (zh) * 2013-07-31 2014-01-01 新乡医学院 一种报告基因重组在染色体目的基因上的方法、位点及用途
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
WO2016100049A1 (en) 2014-12-18 2016-06-23 Edico Genome Corporation Chemically-sensitive field effect transistor
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
EP3459115A4 (en) 2016-05-16 2020-04-08 Agilome, Inc. GRAPHEN-FET DEVICES, SYSTEMS AND METHODS FOR USE THEREOF FOR SEQUENCING NUCLEIC ACIDS

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US568772A (en) * 1896-10-06 Albert s
CA1293460C (en) * 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
GB2187415A (en) 1986-03-05 1987-09-09 Deere & Co Machine for forming cylindrical bales of crop
JPS62265992A (ja) * 1986-05-12 1987-11-18 Ajinomoto Co Inc ヒト細胞を用いる有用物質の製造方法
US4956288A (en) 1988-04-22 1990-09-11 Biogen, Inc. Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
FR2646438B1 (fr) * 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
DK0747485T3 (da) * 1989-11-06 1999-08-16 Cell Genesys Inc Fremstilling af proteiner ved anvendelse af homolog rekombination
DE69031172T2 (de) * 1989-12-22 1998-03-12 Applied Research Systems Modifikation der endogenen genexpression mit hilfe eines regulatorischen elements mittels homologe rekombination
US5272071A (en) * 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
WO1992015694A1 (en) * 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
US6270989B1 (en) * 1991-11-05 2001-08-07 Transkaryotic Therapies, Inc. Protein production and delivery
WO1993015191A1 (en) * 1992-01-24 1993-08-05 Life Technologies, Inc. Modulation of enzyme activities in the in vivo cloning of dna
TW402639B (en) * 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
GB2299336A (en) * 1993-12-23 1996-10-02 Merck & Co Inc Homologous recombination antibody expression sysstem for murine cells
US6130364A (en) * 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
US5882914A (en) 1995-06-06 1999-03-16 The Johns Hopkins University School Of Medicine Nucleic acids encoding the hypoxia inducible factor-1
US5695977A (en) * 1995-08-31 1997-12-09 Genetic Information Research Institute Site directed recombination
AUPN903196A0 (en) * 1996-03-29 1996-04-26 Australian National University, The Single-step excision means
US6020144A (en) * 1996-09-12 2000-02-01 Symbiontics, Inc. Sustained delivery device comprising a Leishmania protozoa and methods of making and using the same

Also Published As

Publication number Publication date
CA2252970A1 (en) 1999-06-01
EP1464705B1 (de) 2006-03-15
DK0919619T4 (da) 2013-09-23
DK0919619T3 (da) 2006-07-10
ATE318904T1 (de) 2006-03-15
EP0919619B1 (de) 2006-03-01
PT1464705E (pt) 2006-08-31
EP0957165B1 (de) 2007-04-18
PT957165E (pt) 2007-07-19
TR199802503A3 (tr) 1999-06-21
EP0957165A3 (de) 2000-10-04
BR9805682A (pt) 2000-04-11
CN1590551A (zh) 2005-03-09
EP0957165A2 (de) 1999-11-17
CA2252970C (en) 2011-11-15
DK1464705T3 (da) 2006-07-24
ZA9810915B (en) 2000-05-30
AU757930B2 (en) 2003-03-13
AU9328498A (en) 1999-06-17
CN1232644C (zh) 2005-12-21
CN1240829A (zh) 2000-01-12
EP0919619A2 (de) 1999-06-02
ATE360067T1 (de) 2007-05-15
DE59813413D1 (de) 2006-04-27
ES2259422T3 (es) 2006-10-01
ES2258809T5 (es) 2013-09-05
AR020892A2 (es) 2002-06-05
AR020750A2 (es) 2002-05-29
KR19990062655A (ko) 1999-07-26
JP4629813B2 (ja) 2011-02-09
EP1464705A1 (de) 2004-10-06
EP0919619A3 (de) 1999-07-21
ES2284228T3 (es) 2007-11-01
DE59813438D1 (de) 2006-05-11
US7008764B1 (en) 2006-03-07
TR199802503A2 (xx) 1999-06-21
ES2258809T3 (es) 2006-09-01
ATE320491T1 (de) 2006-04-15
DE59813976D1 (de) 2007-05-31
AR018022A1 (es) 2001-10-31
JPH11225785A (ja) 1999-08-24
KR100367062B1 (ko) 2004-05-31
CN100519751C (zh) 2009-07-29
DK0957165T3 (da) 2007-07-30
PT919619E (pt) 2006-07-31
EP0919619B2 (de) 2013-06-19

Similar Documents

Publication Publication Date Title
AR020893A2 (es) UN PROCEDIMIENTO PARA PROVEER UNA CÉLULA EUCARIoTICA DHFR NEGATIVA, UN PROCEDIMIENTO PARA INTRODUCIR DE UN GEN DHFR HETERoLOGO EN UNA CÉLULA EUCARIoTICA, UN VECTOR PARA RECOMBINACIoN HOMoLOGA Y UNA CÉLULA EUCARIoTICA, PREFERENTEMENTE UNA CÉLULA HUMANA QUE NO PUEDE DAR LUGAR A UN ORGANISMO ENTERO.
DK0672160T3 (da) Aktivering af ekspression af et amplificerende endogent gen ved homolog rekombination
MX9605542A (es) Produccion de proteinas y liberacion de proteinas.
RU2004107694A (ru) Способ интеграции генов в специфических сайтах в клетках млекопитающих посредством гомологичной рекомбинации и векторы для осуществления этого способа
DE69733121D1 (de) Morphologische mutanten von filamentösen pilzen
AR020331A1 (es) Construccion, y metodo para producir una proteina en una celula de planta
DK1097211T3 (da) Ekspression af eukaryote peptider i planteplastider
DK0843961T3 (da) Kimærisk mus og fremgangsmåde til at producere samme
DK0452484T3 (da) Fremstilling af proteiner ved homolog rekombination
NZ337676A (en) Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same
AR047769A1 (es) Metodos de produccion de apolipoproteinas en las plantas transgenicas
ATE455179T1 (de) Humanes chromosomfragment und ein vektor, der dies enthält
SI9500270B (sl) Rekombinantni mersacidin in postopek za izdelavo
DK0952223T3 (da) Regulatorisk sekvens for cellulase cbh1-gener fra Trichoderma viride og system til masseproduktion af proteiner eller peptider under anvendelse af en sådan sekvens
AR011994A1 (es) Secuencia aislada de acidos nucleicos, vector para la distribucion de dicha secuencia aislada de acidos nucleicos, celula huesped, planta y metodo
WO1999055841A3 (en) Process for obtaining stem cells
DK344884D0 (da) Fremgangsmaade til fremstilling af modne proteiner ud fra fusionsproteiner, syntetiseret i pro- eller eukaryote celler
UY24667A1 (es) Metodo para inmortalizar celulas de pollo
SG146623A1 (en) Rodent expression systems utilising polyoma virus and epstein barr virus sequences
ATE299178T1 (de) Verfahren zur herstellung von säugetier-zellinien
AU2001258400A1 (en) Method for introducing a nucleic acid into a cell (transfection) by means of calcium phosphate, and transfected cell
Manes et al. Hypermethylated tRNA in cleaving rabbit embryos
Grotjahn Oligonucleotide sputtering from liquid matrices
CO4790179A1 (es) Procedimiento para la fabricacion de proteinas humanas por activacion genica endogena
NO20010246D0 (no) Fremgangsmåte for fremstilling av proteiner i transformerte gjærceller

Legal Events

Date Code Title Description
FG Grant, registration