AU2001258400A1 - Method for introducing a nucleic acid into a cell (transfection) by means of calcium phosphate, and transfected cell - Google Patents
Method for introducing a nucleic acid into a cell (transfection) by means of calcium phosphate, and transfected cellInfo
- Publication number
- AU2001258400A1 AU2001258400A1 AU2001258400A AU5840001A AU2001258400A1 AU 2001258400 A1 AU2001258400 A1 AU 2001258400A1 AU 2001258400 A AU2001258400 A AU 2001258400A AU 5840001 A AU5840001 A AU 5840001A AU 2001258400 A1 AU2001258400 A1 AU 2001258400A1
- Authority
- AU
- Australia
- Prior art keywords
- cell
- introducing
- nucleic acid
- calcium phosphate
- transfection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Abstract
The invention relates to a method for introducing a nucleic acid into at least one cell by means of calcium phosphate (CaPi), to a method for producing a transfected cell and to the transfected cell as such.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10024334A DE10024334B4 (en) | 2000-05-17 | 2000-05-17 | Method for introducing nucleic acid into a cell (transfection) by means of calcium phosphate |
DE10024334 | 2000-05-17 | ||
PCT/EP2001/005531 WO2001088171A1 (en) | 2000-05-17 | 2001-05-15 | Method for introducing a nucleic acid into a cell (transfection) by means of calcium phosphate, and transfected cell |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001258400A1 true AU2001258400A1 (en) | 2001-11-26 |
Family
ID=7642499
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001258400A Abandoned AU2001258400A1 (en) | 2000-05-17 | 2001-05-15 | Method for introducing a nucleic acid into a cell (transfection) by means of calcium phosphate, and transfected cell |
Country Status (8)
Country | Link |
---|---|
US (1) | US20040053410A1 (en) |
EP (1) | EP1294919B1 (en) |
AT (1) | ATE333508T1 (en) |
AU (1) | AU2001258400A1 (en) |
CA (1) | CA2409701A1 (en) |
DE (2) | DE10024334B4 (en) |
ES (1) | ES2266197T3 (en) |
WO (1) | WO2001088171A1 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100492292B1 (en) * | 2002-11-11 | 2005-05-27 | 오덕재 | A method for producing calcium-phosphate/nucleic acid complex for transfection |
WO2008145400A2 (en) | 2007-05-31 | 2008-12-04 | Medigene Ag | Mutated structural protein of a parvovirus |
EP2012122A1 (en) * | 2007-07-06 | 2009-01-07 | Medigene AG | Mutated parvovirus structural proteins as vaccines |
US9464119B2 (en) | 2009-03-04 | 2016-10-11 | Deutsches Krebsforschungszentrum | Assembly activating protein (AAP) and its use for the manufacture of parvovirus particles essentially consisting of VP3 |
WO2012031760A1 (en) | 2010-09-08 | 2012-03-15 | Medigene Ag | Parvovirus mutated structural proteins comprising cross - protective b - cell epitopes of a hpv l2 protein as well as products and methods relating thereto |
WO2018208960A1 (en) * | 2017-05-09 | 2018-11-15 | Dimension Therapeutics, Inc. | Scalable method for producing transfection reagents |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1993004169A1 (en) * | 1991-08-20 | 1993-03-04 | Genpharm International, Inc. | Gene targeting in animal cells using isogenic dna constructs |
AU681405B2 (en) * | 1993-06-29 | 1997-08-28 | Chiron Corporation | A truncated keratinocyte growth factor (KGF) having increased biological activity |
ATE228170T1 (en) * | 1994-09-08 | 2002-12-15 | Genentech Inc | METHOD FOR CALCIUM PHOSPHATE TRANSFECTION |
US5976807A (en) * | 1998-03-18 | 1999-11-02 | Pharmacopeia, Inc. | Eukaryotic cells stably expressing genes from multiple transfected episomes |
US20020006664A1 (en) * | 1999-09-17 | 2002-01-17 | Sabatini David M. | Arrayed transfection method and uses related thereto |
US7156579B2 (en) * | 2004-09-02 | 2007-01-02 | Clemson University | Manufactured caverns in carbonate rock |
-
2000
- 2000-05-17 DE DE10024334A patent/DE10024334B4/en not_active Expired - Fee Related
-
2001
- 2001-05-15 EP EP01931696A patent/EP1294919B1/en not_active Expired - Lifetime
- 2001-05-15 US US10/276,207 patent/US20040053410A1/en not_active Abandoned
- 2001-05-15 AT AT01931696T patent/ATE333508T1/en not_active IP Right Cessation
- 2001-05-15 CA CA002409701A patent/CA2409701A1/en not_active Abandoned
- 2001-05-15 ES ES01931696T patent/ES2266197T3/en not_active Expired - Lifetime
- 2001-05-15 DE DE50110507T patent/DE50110507D1/en not_active Expired - Lifetime
- 2001-05-15 WO PCT/EP2001/005531 patent/WO2001088171A1/en active IP Right Grant
- 2001-05-15 AU AU2001258400A patent/AU2001258400A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2001088171A1 (en) | 2001-11-22 |
ES2266197T3 (en) | 2007-03-01 |
DE10024334B4 (en) | 2006-06-01 |
DE50110507D1 (en) | 2006-08-31 |
CA2409701A1 (en) | 2001-11-22 |
DE10024334A1 (en) | 2001-12-06 |
US20040053410A1 (en) | 2004-03-18 |
ATE333508T1 (en) | 2006-08-15 |
EP1294919A1 (en) | 2003-03-26 |
EP1294919B1 (en) | 2006-07-19 |
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