DE69733121D1 - Morphologische mutanten von filamentösen pilzen - Google Patents

Morphologische mutanten von filamentösen pilzen

Info

Publication number
DE69733121D1
DE69733121D1 DE69733121T DE69733121T DE69733121D1 DE 69733121 D1 DE69733121 D1 DE 69733121D1 DE 69733121 T DE69733121 T DE 69733121T DE 69733121 T DE69733121 T DE 69733121T DE 69733121 D1 DE69733121 D1 DE 69733121D1
Authority
DE
Germany
Prior art keywords
cell
mutant
parent
identifying
obtaining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69733121T
Other languages
English (en)
Other versions
DE69733121T2 (de
Inventor
Jeffrey R Shuster
John C Royer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novozymes Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biotech Inc filed Critical Novozymes Biotech Inc
Publication of DE69733121D1 publication Critical patent/DE69733121D1/de
Application granted granted Critical
Publication of DE69733121T2 publication Critical patent/DE69733121T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/69—Aspergillus oryzae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/77—Fusarium

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69733121T 1996-01-19 1997-01-17 Morphologische mutanten von filamentösen pilzen Expired - Lifetime DE69733121T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US1023896P 1996-01-19 1996-01-19
US10238P 1996-01-19
US72611496A 1996-10-04 1996-10-04
US726114 1996-10-04
PCT/US1997/000829 WO1997026330A2 (en) 1996-01-19 1997-01-17 Morphological mutants of filamentous fungi

Publications (2)

Publication Number Publication Date
DE69733121D1 true DE69733121D1 (de) 2005-06-02
DE69733121T2 DE69733121T2 (de) 2006-03-02

Family

ID=26680948

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69733121T Expired - Lifetime DE69733121T2 (de) 1996-01-19 1997-01-17 Morphologische mutanten von filamentösen pilzen

Country Status (9)

Country Link
US (5) US6066493A (de)
EP (3) EP0877801B1 (de)
JP (1) JP3792725B2 (de)
CN (1) CN1231588C (de)
AT (1) ATE294233T1 (de)
AU (1) AU1750697A (de)
DE (1) DE69733121T2 (de)
DK (1) DK0877801T3 (de)
WO (1) WO1997026330A2 (de)

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US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
AU753578B2 (en) 1997-12-22 2002-10-24 Novozymes A/S Carbohydrate oxidase and use thereof in baking
KR100618495B1 (ko) * 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
EP2278016B1 (de) 1999-03-22 2012-09-26 Novozymes Inc. Promotorsequenzen von Fusarium Venenatum und deren Verwendungen
US6361973B1 (en) 1999-03-22 2002-03-26 Novozymes Biotech, Inc. Promoters for expressing genes in a fungal cell
US7189538B2 (en) * 1999-03-24 2007-03-13 Genencor International, Inc. Hyphal growth in fungi
US6995239B1 (en) * 1999-03-24 2006-02-07 Genencor International, Inc. Hyphal growth in fungi
CN1380905A (zh) * 2000-04-13 2002-11-20 马克·阿龙·埃马尔法尔布 丝状真菌中dna表达文库的高通量筛选
US7122330B2 (en) 2000-04-13 2006-10-17 Mark Aaron Emalfarb High-throughput screening of expressed DNA libraries in filamentous fungi
US6558715B1 (en) * 2000-10-31 2003-05-06 Novozymes Biotech, Inc. Methods for using lipases in baking
EP2119773A1 (de) 2000-06-26 2009-11-18 Novozymes A/S Lipolytische Enzyme aus Fusarium und Acremonium Stämmen
US6824798B2 (en) * 2001-09-27 2004-11-30 J. Gregory Koenig Method of preventing veisalgia
DK1495055T3 (da) * 2002-04-18 2013-11-11 Genencor Int Produktion af funktionelle antistoffer i filamentøse svampe
AU2003231028A1 (en) 2002-04-22 2003-11-03 Novozymes Biotech, Inc. Methods for increasing homologous recombination of a nucleic acid sequence
DE60331644D1 (de) 2002-04-22 2010-04-22 Novozymes Inc Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen
US20040043455A1 (en) 2002-08-30 2004-03-04 Novozymes Biotech, Inc. Methods for producing mammalian trypsins
CN101497908B (zh) 2002-11-18 2014-05-21 诺维信股份有限公司 用于在真菌细胞中表达基因的启动子变体
CN100415877C (zh) * 2002-12-24 2008-09-03 池田食研株式会社 辅酶结合型葡萄糖脱氢酶
EP1622921B1 (de) 2003-05-02 2010-06-16 Novozymes Inc. Varianten von beta-glukosidasen
ES2424346T3 (es) 2003-08-25 2013-10-01 Novozymes Inc. Variantes de glucósido hidrolasas
JP4809347B2 (ja) 2004-06-14 2011-11-09 ノボザイムス アクティーゼルスカブ ポリペプチド製造用シグナルペプチド
US20060080393A1 (en) * 2004-10-12 2006-04-13 Cardone Richard J Method for using e-mail documents to create and update address lists
CA2600693A1 (en) * 2005-03-24 2006-09-28 Dsm Ip Assets B.V. Process for microbial production of a valuable compound
CA2604507C (en) 2005-03-25 2015-11-10 Ikeda Food Research Co., Ltd. Coenzyme-linked glucose dehydrogenase and polynucleotide encoding the same
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
WO2008073914A2 (en) 2006-12-10 2008-06-19 Dyadic International Inc. Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi
US20100093061A1 (en) * 2006-12-20 2010-04-15 Bodie Elizabeth A Assays for Improved Fungal Strains
WO2008138835A1 (en) 2007-05-09 2008-11-20 Novozymes A/S Expression cloning method suitable for selecting library clones producing a polypeptide of interest
EP2197893B1 (de) 2007-09-07 2013-07-24 Dyadic International, Inc. Neue pilzenzyme
MX350399B (es) 2009-12-18 2017-09-06 Novozymes Inc Metodos para producir polipeptidos en mutantes de trichoderma deficientes en proteasa.
WO2011161004A1 (en) * 2010-06-22 2011-12-29 Dsm Ip Assets B.V. Air bubble fermentation process
IN2014CN02100A (de) 2011-08-24 2015-05-29 Novozymes Inc
US9493790B2 (en) 2011-08-24 2016-11-15 Novozymes, Inc. Methods for producing multiple recombinant polypeptides in a filamentous fungal host cell
CN103748226A (zh) 2011-08-24 2014-04-23 诺维信股份有限公司 获得丝状真菌宿主细胞的阳性转化体的方法
WO2013028927A1 (en) 2011-08-24 2013-02-28 Novozymes, Inc. Aspergillus fumigatus cellulolytic enzyme compositions and uses thereof
EP3358000A3 (de) 2012-01-05 2018-12-19 Glykos Finland Oy Filamentöse pilzzellen mit proteasemangel und verfahren zur verwendung davon
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
US9598698B2 (en) 2012-08-17 2017-03-21 Novozymes A/S Methods for co-silencing expression of genes in filamentous fungal strains and uses thereof
WO2014100612A1 (en) 2012-12-21 2014-06-26 Novozymes, Inc. Methods for producing heterologous polypeptides in mutants of trichoderma
KR20160035587A (ko) 2013-07-10 2016-03-31 노파르티스 아게 복수개의 프로테아제 결핍 사상형 진균 세포들 및 그의 이용방법
EP3074513A1 (de) 2013-11-26 2016-10-05 Novozymes A/S Enzymzusammensetzungen und verwendungen davon
WO2015187935A1 (en) 2014-06-06 2015-12-10 Novozymes A/S Enzyme compositions and uses thereof
WO2016012468A1 (en) 2014-07-21 2016-01-28 Novartis Ag Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
CN113302305A (zh) 2018-12-12 2021-08-24 诺维信公司 提高丝状真菌细胞在多肽的产生方面的生产力的方法
WO2021148278A1 (en) 2020-01-24 2021-07-29 Novozymes A/S Mutants of a filamentous fungal cell having increased productivity in the production of a polypeptide

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Also Published As

Publication number Publication date
EP2295581A3 (de) 2011-05-04
US6184026B1 (en) 2001-02-06
DE69733121T2 (de) 2006-03-02
EP0877801A2 (de) 1998-11-18
CN1219971A (zh) 1999-06-16
EP1605050A2 (de) 2005-12-14
JP3792725B2 (ja) 2006-07-05
EP0877801B1 (de) 2005-04-27
US6544774B1 (en) 2003-04-08
US7198938B2 (en) 2007-04-03
EP2295581A2 (de) 2011-03-16
US6066493A (en) 2000-05-23
JP2000503537A (ja) 2000-03-28
CN1231588C (zh) 2005-12-14
WO1997026330A2 (en) 1997-07-24
US20040009579A1 (en) 2004-01-15
EP1605050A3 (de) 2006-03-22
WO1997026330A3 (en) 1997-10-09
DK0877801T3 (da) 2005-08-29
US20040266009A1 (en) 2004-12-30
US6794180B2 (en) 2004-09-21
ATE294233T1 (de) 2005-05-15
AU1750697A (en) 1997-08-11

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