DE60331644D1 - Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen - Google Patents

Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen

Info

Publication number
DE60331644D1
DE60331644D1 DE60331644T DE60331644T DE60331644D1 DE 60331644 D1 DE60331644 D1 DE 60331644D1 DE 60331644 T DE60331644 T DE 60331644T DE 60331644 T DE60331644 T DE 60331644T DE 60331644 D1 DE60331644 D1 DE 60331644D1
Authority
DE
Germany
Prior art keywords
dna sequence
plasmids
region
dna
variants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60331644T
Other languages
English (en)
Inventor
Howard Brody
Suzanne Otani
Joel Cherry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of DE60331644D1 publication Critical patent/DE60331644D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60331644T 2002-04-22 2003-04-22 Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen Expired - Lifetime DE60331644D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37468802P 2002-04-22 2002-04-22
PCT/US2003/013577 WO2003089648A1 (en) 2002-04-22 2003-04-22 Methods for preparing variants of a dna sequence in filamentous fungi

Publications (1)

Publication Number Publication Date
DE60331644D1 true DE60331644D1 (de) 2010-04-22

Family

ID=29251220

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60331644T Expired - Lifetime DE60331644D1 (de) 2002-04-22 2003-04-22 Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen

Country Status (7)

Country Link
US (1) US20030199038A1 (de)
EP (1) EP1499733B1 (de)
AT (1) ATE460488T1 (de)
AU (1) AU2003231228A1 (de)
DE (1) DE60331644D1 (de)
DK (1) DK1499733T3 (de)
WO (1) WO2003089648A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7939304B2 (en) * 2002-10-02 2011-05-10 Catalyst Biosciences, Inc. Mutant MT-SP1 proteases with altered substrate specificity or activity
US20060024289A1 (en) * 2002-10-02 2006-02-02 Ruggles Sandra W Cleavage of VEGF and VEGF receptor by wild-type and mutant proteases
KR101132555B1 (ko) * 2002-10-02 2012-04-02 리전츠 오브 더 유니버시티 오브 캘리포니아 변경된 특이성을 갖는 프로테아제의 제조 및 선별 방법
EP2308967B1 (de) 2004-04-12 2017-08-23 Catalyst Biosciences, Inc. Mutante mt-sp1 polypeptide
EP1777292A1 (de) * 2005-10-19 2007-04-25 Signalomics GmbH Verfahren zum Erzeugen von genetischer Diversität in vivo
US10337019B2 (en) * 2016-01-25 2019-07-02 Intact Genomics, Inc. Fungal artificial chromosomes, compositions, methods and uses therefor

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4518584A (en) * 1983-04-15 1985-05-21 Cetus Corporation Human recombinant interleukin-2 muteins
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0208491B1 (de) * 1985-07-03 1993-08-25 Genencor International, Inc. Hybride Polypeptide und Verfahren zu deren Herstellung
US4894331A (en) * 1985-09-27 1990-01-16 Amgen Inc. Partial marker cassette mutagenesis of xylose isomerase
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB9114734D0 (en) 1991-07-09 1991-08-28 Univ London Process for modifying proteins
BR9507817A (pt) 1994-06-03 1997-09-16 Novo Nordisk Biotech Inc Construção de dna enzima vetor recombinante célula hospedeira recombinante lacase de ascomicete ou deuteromicete processos para obter uma enzima de lacase para melhorar o rendimento de enzima recombinante para polimerizar um substrato de lignina ou lignossulfato em soluç o para despolimerizar in situ a pasta kraft para oxidar corantes ou precursores de corantes para pintar cabelo e para polimerizar ou oxidar um composto fenólico ou anilina composição de corante e recipiente contendo a mesma
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
AU6655396A (en) * 1995-08-11 1997-03-12 Novo Nordisk A/S Method for preparing polypeptide variants
DK0877801T3 (da) 1996-01-19 2005-08-29 Novozymes Biotech Inc Morfologiske mutanter af trådsvampe
ATE265534T1 (de) 1996-06-10 2004-05-15 Novozymes Biotech Inc Verfahren zur vermehrung der hemoproteinherstellung in filamentösen fungi
EP0910627A1 (de) 1996-06-28 1999-04-28 Novo Nordisk A/S Rekombinantes enzym mit dextranase aktivität
US5958727A (en) 1996-09-13 1999-09-28 Novo Nordisk Biotech, Inc Methods for modifying the production of a polypeptide
JP2002516116A (ja) 1998-05-27 2002-06-04 ノボザイムス バイオテック，インコーポレイティド 遺伝子のコピー数を変化させることによりポリペプチドを生産するための方法
DE69932345T2 (de) 1998-10-26 2007-07-19 Novozymes A/S Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
JP2003502015A (ja) * 1999-03-17 2003-01-21 パラダイム ジェネティックス、 インコーポレイテッド 生物中における遺伝子ノックアウトライブラリの迅速かつ大量産生のための方法類および素材類

Also Published As

Publication number Publication date
WO2003089648A1 (en) 2003-10-30
US20030199038A1 (en) 2003-10-23
EP1499733B1 (de) 2010-03-10
EP1499733A1 (de) 2005-01-26
DK1499733T3 (da) 2010-07-12
AU2003231228A1 (en) 2003-11-03
EP1499733A4 (de) 2005-09-14
ATE460488T1 (de) 2010-03-15

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