ATE527366T1 - Verfahren zur mischung von vielen heterologen genen - Google Patents
Verfahren zur mischung von vielen heterologen genenInfo
- Publication number
- ATE527366T1 ATE527366T1 AT03787738T AT03787738T ATE527366T1 AT E527366 T1 ATE527366 T1 AT E527366T1 AT 03787738 T AT03787738 T AT 03787738T AT 03787738 T AT03787738 T AT 03787738T AT E527366 T1 ATE527366 T1 AT E527366T1
- Authority
- AT
- Austria
- Prior art keywords
- methods
- cells
- heterologous genes
- genes
- evolution
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA200201173 | 2002-08-01 | ||
DKPA200300787 | 2003-05-23 | ||
PCT/DK2003/000521 WO2004016791A1 (en) | 2002-08-01 | 2003-08-01 | Methods of mixing large numbers of heterologous genes |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE527366T1 true ATE527366T1 (de) | 2011-10-15 |
Family
ID=31889347
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03787738T ATE527366T1 (de) | 2002-08-01 | 2003-08-01 | Verfahren zur mischung von vielen heterologen genen |
Country Status (6)
Country | Link |
---|---|
US (1) | US20060252156A1 (de) |
EP (1) | EP1529110B1 (de) |
AT (1) | ATE527366T1 (de) |
AU (1) | AU2003247270A1 (de) |
DK (1) | DK1529110T3 (de) |
WO (1) | WO2004016791A1 (de) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8008459B2 (en) * | 2001-01-25 | 2011-08-30 | Evolva Sa | Concatemers of differentially expressed multiple genes |
WO2002059297A2 (en) * | 2001-01-25 | 2002-08-01 | Evolva Biotech A/S | A library of a collection of cells |
WO2003062419A1 (en) * | 2002-01-25 | 2003-07-31 | Evolva Ltd | Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities |
CA2958259C (en) | 2004-10-22 | 2020-06-30 | Revivicor, Inc. | Ungulates with genetically modified immune systems |
ES2546484T3 (es) | 2005-03-18 | 2015-09-24 | Dsm Ip Assets B.V. | Producción de carotenoides en levadura y hongos oleaginosos |
RU2468820C2 (ru) * | 2006-07-26 | 2012-12-10 | Интрексон Корпорейшн | Способы и композиции для лечения заболевания |
WO2008042338A2 (en) | 2006-09-28 | 2008-04-10 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
CN101343630B (zh) * | 2007-07-12 | 2012-11-07 | 中国科学院上海生命科学研究院 | 利用基因组改组技术改良多拉菌素生产菌的方法 |
EP2348827B1 (de) | 2008-10-27 | 2015-07-01 | Revivicor, Inc. | Immunsupprimierte huftiere |
WO2011146833A1 (en) | 2010-05-20 | 2011-11-24 | Evolva Inc. | Method of producing isoprenoid compounds in yeast |
US20140308699A1 (en) | 2010-07-30 | 2014-10-16 | Glycode | Yeast artificial chromosome carrying the mammalian glycosylation pathway |
CN107287185B (zh) * | 2017-07-22 | 2021-05-18 | 贵州省园艺研究所 | 马铃薯四倍体栽培种与二倍体野生种原生质体融合方法 |
Family Cites Families (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US41333A (en) * | 1864-01-19 | Improvement in spinning-rollers | ||
US25517A (en) * | 1859-09-20 | Improved method of making soap | ||
US158860A (en) * | 1875-01-19 | Improvement in hame attachments | ||
US19924A (en) * | 1858-04-13 | Improvement in seed-drills | ||
US5316922A (en) * | 1992-04-15 | 1994-05-31 | The United States Of America As Represented By Department Of Health And Human Services | Method for indentifying and expressing proteins that recognize and adhere to specific probes |
US6268140B1 (en) * | 1994-02-03 | 2001-07-31 | Neugenesis | Combinatorial metabolic libraries |
US6165793A (en) * | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5837458A (en) * | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US6309883B1 (en) * | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5824485A (en) * | 1995-04-24 | 1998-10-20 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
US6057103A (en) * | 1995-07-18 | 2000-05-02 | Diversa Corporation | Screening for novel bioactivities |
US5958672A (en) * | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
US6030779A (en) * | 1995-07-18 | 2000-02-29 | Diversa Corporation | Screening for novel bioactivities |
US6004788A (en) * | 1995-07-18 | 1999-12-21 | Diversa Corporation | Enzyme kits and libraries |
US5939250A (en) * | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
US20030215798A1 (en) * | 1997-06-16 | 2003-11-20 | Diversa Corporation | High throughput fluorescence-based screening for novel enzymes |
GB9603803D0 (en) * | 1996-02-22 | 1996-04-24 | Zeneca Ltd | Production of proteins, plasmids coding therefor and organisms containing such plasmids |
US6025155A (en) * | 1996-04-10 | 2000-02-15 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
US5783431A (en) * | 1996-04-24 | 1998-07-21 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
US6242211B1 (en) | 1996-04-24 | 2001-06-05 | Terragen Discovery, Inc. | Methods for generating and screening novel metabolic pathways |
US5763239A (en) * | 1996-06-18 | 1998-06-09 | Diversa Corporation | Production and use of normalized DNA libraries |
EP1717322B1 (de) | 1997-01-17 | 2012-07-18 | Codexis Mayflower Holdings, LLC | Evolution ganzer Zellen durch rekursive Sequenzrekombination |
US6326204B1 (en) * | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
GB9908814D0 (en) * | 1999-04-16 | 1999-06-09 | Celltech Therapeutics Ltd | Process |
DE60036715T2 (de) | 1999-07-28 | 2008-07-17 | Nippon Kayaku K.K. | Gasgenerator |
WO2001073000A2 (en) * | 2000-03-24 | 2001-10-04 | Maxygen, Inc. | Methods for modulating cellular and organismal phenotypes |
US7351813B2 (en) * | 2000-06-20 | 2008-04-01 | The Board Of Trustees Of The Leland Stanford Junior University | Liver-specific gene expression cassettes, and methods of use |
US20030175678A1 (en) * | 2001-01-24 | 2003-09-18 | Bowen Benjamin A. | Methods for identifying genes regulating desired cell phenotypes |
WO2002059297A2 (en) * | 2001-01-25 | 2002-08-01 | Evolva Biotech A/S | A library of a collection of cells |
US8008459B2 (en) * | 2001-01-25 | 2011-08-30 | Evolva Sa | Concatemers of differentially expressed multiple genes |
JP2004527232A (ja) * | 2001-01-25 | 2004-09-09 | エボルバ バイオテック アクティーゼルスカブ | 所望の表現型を有する細胞に進化させる方法及び進化した細胞 |
AU2002227882B2 (en) * | 2001-01-25 | 2007-06-28 | Evolva Ltd | Concatemers of differentially expressed multiple genes |
WO2003062419A1 (en) * | 2002-01-25 | 2003-07-31 | Evolva Ltd | Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities |
EP1558741B1 (de) * | 2002-06-14 | 2008-02-20 | Dyax Corporation | Rekombination von nukleinsäurebibliotheksmitgliedern |
US20030235823A1 (en) * | 2002-06-24 | 2003-12-25 | The University Of Alabama | Nucleotide sequences that code for torsin genes, torsin proteins, and methods of using the same to treat protein-aggregation |
-
2003
- 2003-08-01 DK DK03787738.8T patent/DK1529110T3/da active
- 2003-08-01 WO PCT/DK2003/000521 patent/WO2004016791A1/en not_active Application Discontinuation
- 2003-08-01 AU AU2003247270A patent/AU2003247270A1/en not_active Abandoned
- 2003-08-01 AT AT03787738T patent/ATE527366T1/de not_active IP Right Cessation
- 2003-08-01 EP EP03787738A patent/EP1529110B1/de not_active Expired - Lifetime
- 2003-08-01 US US10/523,007 patent/US20060252156A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
DK1529110T3 (da) | 2012-01-30 |
EP1529110A1 (de) | 2005-05-11 |
US20060252156A1 (en) | 2006-11-09 |
EP1529110B1 (de) | 2011-10-05 |
WO2004016791A1 (en) | 2004-02-26 |
AU2003247270A1 (en) | 2004-03-03 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |