DK1529110T3 - Fremgangsmåde til blanding af store antal af heterologe gener - Google Patents

Fremgangsmåde til blanding af store antal af heterologe gener

Info

Publication number
DK1529110T3
DK1529110T3 DK03787738.8T DK03787738T DK1529110T3 DK 1529110 T3 DK1529110 T3 DK 1529110T3 DK 03787738 T DK03787738 T DK 03787738T DK 1529110 T3 DK1529110 T3 DK 1529110T3
Authority
DK
Denmark
Prior art keywords
heterologous genes
methods
cells
large numbers
evolution
Prior art date
Application number
DK03787738.8T
Other languages
English (en)
Inventor
Neil Goldsmith
Alexandra M P Santana Sorensen
Soren V S Nielsen
Curt Aime Friis Nielsen
Original Assignee
Evolva Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Ltd filed Critical Evolva Ltd
Application granted granted Critical
Publication of DK1529110T3 publication Critical patent/DK1529110T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Processing And Handling Of Plastics And Other Materials For Molding In General (AREA)
DK03787738.8T 2002-08-01 2003-08-01 Fremgangsmåde til blanding af store antal af heterologe gener DK1529110T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200201173 2002-08-01
DKPA200300787 2003-05-23
PCT/DK2003/000521 WO2004016791A1 (en) 2002-08-01 2003-08-01 Methods of mixing large numbers of heterologous genes

Publications (1)

Publication Number Publication Date
DK1529110T3 true DK1529110T3 (da) 2012-01-30

Family

ID=31889347

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03787738.8T DK1529110T3 (da) 2002-08-01 2003-08-01 Fremgangsmåde til blanding af store antal af heterologe gener

Country Status (6)

Country Link
US (1) US20060252156A1 (da)
EP (1) EP1529110B1 (da)
AT (1) ATE527366T1 (da)
AU (1) AU2003247270A1 (da)
DK (1) DK1529110T3 (da)
WO (1) WO2004016791A1 (da)

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US7838287B2 (en) * 2001-01-25 2010-11-23 Evolva Sa Library of a collection of cells
WO2003062419A1 (en) * 2002-01-25 2003-07-31 Evolva Ltd Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities
CN101389214A (zh) 2004-10-22 2009-03-18 雷维维科公司 具有基因修饰免疫系统的有蹄类动物
EA016258B1 (ru) 2005-03-18 2012-03-30 Микробиа, Инк. Рекомбинантные грибы, продуцирующие каротиноиды, и способы их применения
AU2007332980A1 (en) * 2006-07-26 2008-06-19 Intrexon Corporation Methods and compositions for treating disease
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CN101343630B (zh) * 2007-07-12 2012-11-07 中国科学院上海生命科学研究院 利用基因组改组技术改良多拉菌素生产菌的方法
EP2348827B1 (en) 2008-10-27 2015-07-01 Revivicor, Inc. Immunocompromised ungulates
EP2571991A1 (en) 2010-05-20 2013-03-27 Evolva SA Method of producing isoprenoid compounds in yeast
EP2598638A2 (en) 2010-07-30 2013-06-05 Glycode A yeast artificial chromosome carrying the mammalian glycosylation pathway
CN107287185B (zh) * 2017-07-22 2021-05-18 贵州省园艺研究所 马铃薯四倍体栽培种与二倍体野生种原生质体融合方法

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US7838287B2 (en) * 2001-01-25 2010-11-23 Evolva Sa Library of a collection of cells
DE60228935D1 (de) * 2001-01-25 2008-10-30 Evolva Ltd Verfahren zur entwicklung einer zelle mit einem gewünschten phänotyp und entwickelte zellen
AU2002227882B2 (en) * 2001-01-25 2007-06-28 Evolva Ltd Concatemers of differentially expressed multiple genes
WO2003062419A1 (en) * 2002-01-25 2003-07-31 Evolva Ltd Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities
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Also Published As

Publication number Publication date
AU2003247270A1 (en) 2004-03-03
WO2004016791A1 (en) 2004-02-26
US20060252156A1 (en) 2006-11-09
EP1529110A1 (en) 2005-05-11
EP1529110B1 (en) 2011-10-05
ATE527366T1 (de) 2011-10-15

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