EA200101186A1 - Регуляция экспрессии вирусных генов - Google Patents

Регуляция экспрессии вирусных генов

Info

Publication number
EA200101186A1
EA200101186A1 EA200101186A EA200101186A EA200101186A1 EA 200101186 A1 EA200101186 A1 EA 200101186A1 EA 200101186 A EA200101186 A EA 200101186A EA 200101186 A EA200101186 A EA 200101186A EA 200101186 A1 EA200101186 A1 EA 200101186A1
Authority
EA
Eurasian Patent Office
Prior art keywords
regulation
application
viral genes
expression
gene
Prior art date
Application number
EA200101186A
Other languages
English (en)
Inventor
Питер Бернард Хайфетц
Дейвид Эндрью Паттон
Йошуа Зви Левин
Цюдэн Цюе
Петрус Теодорус Де-Хан
Йоханнис Якобус Гиелен
Original Assignee
Зингента Партисипейшнс Аг
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/309,038 external-priority patent/US7019195B1/en
Application filed by Зингента Партисипейшнс Аг filed Critical Зингента Партисипейшнс Аг
Publication of EA200101186A1 publication Critical patent/EA200101186A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

Abstract

В заявке описаны способы изменения экспрессии вирусного гена в клетке с использованием смыслового и антисмыслового фрагментов РНК гена. Смысловой и антисмысловой фрагменты РНК обладают способностью спариваться и формировать двухцепочечную молекулу РНК, тем самым изменяя экспрессию гена. В заявке также описаны клетки, растения или животные, их потомство и их семена, полученные с помощью способа по настоящему изобретению. Предпочтительно такие клетки, растения или животные обладают устойчивостью или толерантностью к вирусам.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200101186A 1999-05-10 2000-05-08 Регуляция экспрессии вирусных генов EA200101186A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/309,038 US7019195B1 (en) 1998-05-26 1999-05-10 Method for conferring resistance or tolerance aganist furovirus, potyvirus, tospovirus, and cucomovirus to plant cells
PCT/EP2000/004117 WO2000068374A1 (en) 1999-05-10 2000-05-08 Regulation of viral gene expression

Publications (1)

Publication Number Publication Date
EA200101186A1 true EA200101186A1 (ru) 2002-04-25

Family

ID=23196412

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200101186A EA200101186A1 (ru) 1999-05-10 2000-05-08 Регуляция экспрессии вирусных генов

Country Status (18)

Country Link
EP (2) EP1801215B1 (ru)
JP (3) JP2002543783A (ru)
KR (1) KR20010112952A (ru)
CN (2) CN100420748C (ru)
AR (1) AR023885A1 (ru)
AU (1) AU4563300A (ru)
BR (1) BR0010496A (ru)
CA (1) CA2369422A1 (ru)
CZ (1) CZ20014028A3 (ru)
EA (1) EA200101186A1 (ru)
HK (2) HK1045325A1 (ru)
HU (1) HUP0201235A3 (ru)
IL (2) IL145538A0 (ru)
PL (1) PL351385A1 (ru)
SK (1) SK16222001A3 (ru)
TR (1) TR200103088T2 (ru)
WO (1) WO2000068374A1 (ru)
ZA (1) ZA200109152B (ru)

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Also Published As

Publication number Publication date
KR20010112952A (ko) 2001-12-22
JP2010088435A (ja) 2010-04-22
EP1801215A3 (en) 2008-12-17
AR023885A1 (es) 2002-09-04
PL351385A1 (en) 2003-04-07
SK16222001A3 (sk) 2002-04-04
HUP0201235A3 (en) 2004-10-28
CA2369422A1 (en) 2000-11-16
IL145538A0 (en) 2002-06-30
TR200103088T2 (tr) 2002-05-21
BR0010496A (pt) 2002-04-02
WO2000068374A1 (en) 2000-11-16
HUP0201235A2 (en) 2002-08-28
AU4563300A (en) 2000-11-21
HK1047769B (zh) 2009-08-14
JP2009022291A (ja) 2009-02-05
CN100420748C (zh) 2008-09-24
CN101348785A (zh) 2009-01-21
EP1801215A2 (en) 2007-06-27
JP2002543783A (ja) 2002-12-24
ZA200109152B (en) 2002-09-06
EP1177283A1 (en) 2002-02-06
CN1357040A (zh) 2002-07-03
EP1801215B1 (en) 2013-02-27
CZ20014028A3 (cs) 2002-02-13
HK1045325A1 (zh) 2002-11-22
IL145538A (en) 2010-11-30
HK1047769A1 (en) 2003-03-07

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