ATE460488T1 - Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen - Google Patents

Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen

Info

Publication number
ATE460488T1
ATE460488T1 AT03724363T AT03724363T ATE460488T1 AT E460488 T1 ATE460488 T1 AT E460488T1 AT 03724363 T AT03724363 T AT 03724363T AT 03724363 T AT03724363 T AT 03724363T AT E460488 T1 ATE460488 T1 AT E460488T1
Authority
AT
Austria
Prior art keywords
dna sequence
plasmids
region
dna
variants
Prior art date
Application number
AT03724363T
Other languages
English (en)
Inventor
Howard Brody
Suzanne Otani
Joel Cherry
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of ATE460488T1 publication Critical patent/ATE460488T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03724363T 2002-04-22 2003-04-22 Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen ATE460488T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37468802P 2002-04-22 2002-04-22
PCT/US2003/013577 WO2003089648A1 (en) 2002-04-22 2003-04-22 Methods for preparing variants of a dna sequence in filamentous fungi

Publications (1)

Publication Number Publication Date
ATE460488T1 true ATE460488T1 (de) 2010-03-15

Family

ID=29251220

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03724363T ATE460488T1 (de) 2002-04-22 2003-04-22 Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen

Country Status (7)

Country Link
US (1) US20030199038A1 (de)
EP (1) EP1499733B1 (de)
AT (1) ATE460488T1 (de)
AU (1) AU2003231228A1 (de)
DE (1) DE60331644D1 (de)
DK (1) DK1499733T3 (de)
WO (1) WO2003089648A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5376747B2 (ja) * 2002-10-02 2013-12-25 カタリスト バイオサイエンシーズ， インコーポレイテッド 改変された特異性を有するプロテアーゼを作製する方法およびスクリーニングする方法
US20060024289A1 (en) * 2002-10-02 2006-02-02 Ruggles Sandra W Cleavage of VEGF and VEGF receptor by wild-type and mutant proteases
US7939304B2 (en) * 2002-10-02 2011-05-10 Catalyst Biosciences, Inc. Mutant MT-SP1 proteases with altered substrate specificity or activity
WO2005110453A2 (en) * 2004-04-12 2005-11-24 Catalyst Biosciences, Inc. Cleavage of vegf and vegf receptor by wildtype and mutant mt-sp1
EP1777292A1 (de) * 2005-10-19 2007-04-25 Signalomics GmbH Verfahren zum Erzeugen von genetischer Diversität in vivo
US10337019B2 (en) * 2016-01-25 2019-07-02 Intact Genomics, Inc. Fungal artificial chromosomes, compositions, methods and uses therefor

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4518584A (en) 1983-04-15 1985-05-21 Cetus Corporation Human recombinant interleukin-2 muteins
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0208491B1 (de) * 1985-07-03 1993-08-25 Genencor International, Inc. Hybride Polypeptide und Verfahren zu deren Herstellung
US4894331A (en) * 1985-09-27 1990-01-16 Amgen Inc. Partial marker cassette mutagenesis of xylose isomerase
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB9114734D0 (en) 1991-07-09 1991-08-28 Univ London Process for modifying proteins
EP0765394B1 (de) 1994-06-03 2001-10-04 Novo Nordisk Biotech, Inc. Gereinigte myceliophthora laccasen und nukleinsäuren dafür kodierend
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
JP4156666B2 (ja) * 1995-08-11 2008-09-24 ノボザイムス アクティーゼルスカブ ポリペプチド変異体を調整するための方法
JP3792725B2 (ja) 1996-01-19 2006-07-05 ノボザイムス，インコーポレイティド 糸状菌の形態変異体
DE69728878T2 (de) 1996-06-10 2005-04-07 Novozymes Biotech, Inc., Davis Verfahren zur vermehrung der hemoproteinherstellung in filamentösen fungi
CN1226283A (zh) 1996-06-28 1999-08-18 诺沃挪第克公司 具有葡聚糖酶活性的重组酶
US5958727A (en) 1996-09-13 1999-09-28 Novo Nordisk Biotech, Inc Methods for modifying the production of a polypeptide
JP2002516116A (ja) 1998-05-27 2002-06-04 ノボザイムス バイオテック，インコーポレイティド 遺伝子のコピー数を変化させることによりポリペプチドを生産するための方法
WO2000024883A1 (en) 1998-10-26 2000-05-04 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
JP2003502015A (ja) * 1999-03-17 2003-01-21 パラダイム ジェネティックス、 インコーポレイテッド 生物中における遺伝子ノックアウトライブラリの迅速かつ大量産生のための方法類および素材類

Also Published As

Publication number Publication date
US20030199038A1 (en) 2003-10-23
WO2003089648A1 (en) 2003-10-30
DK1499733T3 (da) 2010-07-12
EP1499733B1 (de) 2010-03-10
EP1499733A4 (de) 2005-09-14
AU2003231228A1 (en) 2003-11-03
EP1499733A1 (de) 2005-01-26
DE60331644D1 (de) 2010-04-22

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