ATE460488T1 - Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen - Google Patents
Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzenInfo
- Publication number
- ATE460488T1 ATE460488T1 AT03724363T AT03724363T ATE460488T1 AT E460488 T1 ATE460488 T1 AT E460488T1 AT 03724363 T AT03724363 T AT 03724363T AT 03724363 T AT03724363 T AT 03724363T AT E460488 T1 ATE460488 T1 AT E460488T1
- Authority
- AT
- Austria
- Prior art keywords
- dna sequence
- plasmids
- region
- dna
- variants
- Prior art date
Links
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 8
- 241000233866 Fungi Species 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 239000013612 plasmid Substances 0.000 abstract 7
- 239000012634 fragment Substances 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 3
- 230000002538 fungal effect Effects 0.000 abstract 3
- 230000003362 replicative effect Effects 0.000 abstract 2
- 230000029087 digestion Effects 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 230000006798 recombination Effects 0.000 abstract 1
- 238000005215 recombination Methods 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37468802P | 2002-04-22 | 2002-04-22 | |
| PCT/US2003/013577 WO2003089648A1 (en) | 2002-04-22 | 2003-04-22 | Methods for preparing variants of a dna sequence in filamentous fungi |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE460488T1 true ATE460488T1 (de) | 2010-03-15 |
Family
ID=29251220
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03724363T ATE460488T1 (de) | 2002-04-22 | 2003-04-22 | Verfahren zur herstellung von varianten einer dna-sequenz in filamentösen pilzen |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20030199038A1 (de) |
| EP (1) | EP1499733B1 (de) |
| AT (1) | ATE460488T1 (de) |
| AU (1) | AU2003231228A1 (de) |
| DE (1) | DE60331644D1 (de) |
| DK (1) | DK1499733T3 (de) |
| WO (1) | WO2003089648A1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7939304B2 (en) * | 2002-10-02 | 2011-05-10 | Catalyst Biosciences, Inc. | Mutant MT-SP1 proteases with altered substrate specificity or activity |
| US20060024289A1 (en) * | 2002-10-02 | 2006-02-02 | Ruggles Sandra W | Cleavage of VEGF and VEGF receptor by wild-type and mutant proteases |
| US20040146938A1 (en) * | 2002-10-02 | 2004-07-29 | Jack Nguyen | Methods of generating and screening for proteases with altered specificity |
| CA2562729C (en) * | 2004-04-12 | 2013-11-12 | Sandra Waugh Ruggles | Cleavage of vegf and vegf receptor by wildtype and mutant mt-sp1 |
| EP1777292A1 (de) * | 2005-10-19 | 2007-04-25 | Signalomics GmbH | Verfahren zum Erzeugen von genetischer Diversität in vivo |
| US10337019B2 (en) * | 2016-01-25 | 2019-07-02 | Intact Genomics, Inc. | Fungal artificial chromosomes, compositions, methods and uses therefor |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4518584A (en) | 1983-04-15 | 1985-05-21 | Cetus Corporation | Human recombinant interleukin-2 muteins |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| DE3688920D1 (de) * | 1985-07-03 | 1993-09-30 | Genencor Int | Hybride Polypeptide und Verfahren zu deren Herstellung. |
| US4894331A (en) * | 1985-09-27 | 1990-01-16 | Amgen Inc. | Partial marker cassette mutagenesis of xylose isomerase |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| GB9114734D0 (en) | 1991-07-09 | 1991-08-28 | Univ London | Process for modifying proteins |
| AU694954B2 (en) | 1994-06-03 | 1998-08-06 | Novo Nordisk A/S | Purified myceliophthora laccases and nucleic acids encoding same |
| ATE294871T1 (de) | 1994-06-30 | 2005-05-15 | Novozymes Biotech Inc | Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren |
| WO1997007206A1 (en) * | 1995-08-11 | 1997-02-27 | Okkels, Jens, Sigurd | Method for preparing polypeptide variants |
| EP2295581A3 (de) | 1996-01-19 | 2011-05-04 | Novozymes Biotech, Inc. | Morphologische Mutanten von filamentösen Pilzen |
| US6100057A (en) | 1996-06-10 | 2000-08-08 | Novo Nordisk Biotech, Inc. | Method for increasing hemoprotein production in filamentous fungi |
| JP2000514289A (ja) | 1996-06-28 | 2000-10-31 | ノボ ノルディスク アクティーゼルスカブ | デキストラナーゼ活性を有する組換え酵素 |
| WO1998011203A1 (en) | 1996-09-13 | 1998-03-19 | Novo Nordisk Biotech, Inc. | Cells having dna insertion mutations which produce altered amounts of a polypeptide |
| JP2002516116A (ja) | 1998-05-27 | 2002-06-04 | ノボザイムス バイオテック，インコーポレイティド | 遺伝子のコピー数を変化させることによりポリペプチドを生産するための方法 |
| CA2344619C (en) | 1998-10-26 | 2012-01-03 | Novozymes A/S | Constructing and screening a dna library of interest in filamentous fungal cells |
| EP1161551A2 (de) * | 1999-03-17 | 2001-12-12 | Paradigm Genetics Inc. | Methoden und materialien zur schnellen und in einer grossen ausbeute resultierenden herstellung von "knock-out" genbanken in einem organismus |
-
2003
- 2003-04-22 AU AU2003231228A patent/AU2003231228A1/en not_active Abandoned
- 2003-04-22 DE DE60331644T patent/DE60331644D1/de not_active Expired - Lifetime
- 2003-04-22 EP EP03724363A patent/EP1499733B1/de not_active Expired - Lifetime
- 2003-04-22 US US10/422,013 patent/US20030199038A1/en not_active Abandoned
- 2003-04-22 AT AT03724363T patent/ATE460488T1/de not_active IP Right Cessation
- 2003-04-22 DK DK03724363.1T patent/DK1499733T3/da active
- 2003-04-22 WO PCT/US2003/013577 patent/WO2003089648A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| DE60331644D1 (de) | 2010-04-22 |
| EP1499733B1 (de) | 2010-03-10 |
| WO2003089648A1 (en) | 2003-10-30 |
| US20030199038A1 (en) | 2003-10-23 |
| EP1499733A4 (de) | 2005-09-14 |
| AU2003231228A1 (en) | 2003-11-03 |
| EP1499733A1 (de) | 2005-01-26 |
| DK1499733T3 (da) | 2010-07-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |