ATE293684T1 - Methode zur invivo produktion einer mutanten- bibliothek in zellen - Google Patents

Methode zur invivo produktion einer mutanten- bibliothek in zellen

Info

Publication number
ATE293684T1
ATE293684T1 AT97900206T AT97900206T ATE293684T1 AT E293684 T1 ATE293684 T1 AT E293684T1 AT 97900206 T AT97900206 T AT 97900206T AT 97900206 T AT97900206 T AT 97900206T AT E293684 T1 ATE293684 T1 AT E293684T1
Authority
AT
Austria
Prior art keywords
cells
replication
production
invivo
genetic
Prior art date
Application number
AT97900206T
Other languages
English (en)
Inventor
Torben Vedel Borchert
Stanislas Dusko Ehrlich
Agronomique
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE293684T1 publication Critical patent/ATE293684T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
AT97900206T 1996-01-10 1997-01-10 Methode zur invivo produktion einer mutanten- bibliothek in zellen ATE293684T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK1896 1996-01-10
PCT/DK1997/000014 WO1997025410A1 (en) 1996-01-10 1997-01-10 A method for in vivo production of a mutant library in cells

Publications (1)

Publication Number Publication Date
ATE293684T1 true ATE293684T1 (de) 2005-05-15

Family

ID=8088824

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97900206T ATE293684T1 (de) 1996-01-10 1997-01-10 Methode zur invivo produktion einer mutanten- bibliothek in zellen

Country Status (8)

Country Link
EP (1) EP0873398B1 (de)
JP (1) JP2000502568A (de)
CN (2) CN1210557A (de)
AT (1) ATE293684T1 (de)
AU (1) AU1367897A (de)
DE (1) DE69733059T2 (de)
DK (1) DK0873398T3 (de)
WO (1) WO1997025410A1 (de)

Families Citing this family (39)

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Publication number Priority date Publication date Assignee Title
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
IL130635A0 (en) 1997-01-17 2000-06-01 Maxygen Inc Evolution of whole cells and organisms by recursive sequence recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
EP1854890A1 (de) * 1997-12-22 2007-11-14 DSM IP Assets B.V. Expressionsklonierung in filamentösen Pilzen
BR9910174A (pt) 1998-05-01 2001-03-06 Maxygen Inc Processo para se obter um gene recombinante otimizado de resistência à praga, biblioteca, e, processo para se obter um organismo que seja patogênico a uma praga de vegetal
AU5347999A (en) 1998-08-12 2000-03-06 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
US6500639B2 (en) 1998-10-07 2002-12-31 Maxygen, Inc. DNA shuffling to produce nucleic acids for mycotoxin detoxification
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
EP1108781A3 (de) 1999-01-19 2001-09-12 Maxygen, Inc. Verfahren zum Erfassen von Zeichenketten, Polynukleotiden und Polypeptiden
KR20010102069A (ko) 1999-02-11 2001-11-15 추후제출 고효율 질량 분석
EP1165775A2 (de) 1999-03-05 2002-01-02 Maxygen, Inc. Rekombination von insertionsmodifizierten nucleinsäuren
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
WO2002000717A2 (en) 2000-06-23 2002-01-03 Maxygen, Inc. Novel co-stimulatory molecules
EP1297146A2 (de) 2000-06-23 2003-04-02 Maxygen, Inc. Chimäre promotoren
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
JP4647117B2 (ja) * 2001-03-07 2011-03-09 セレスター・レキシコ・サイエンシズ株式会社 突然変異誘発方法
AU2003258658A1 (en) * 2002-08-29 2004-03-19 Andreas Beck Improved dna and proteins
DE102005037351B3 (de) * 2005-08-08 2007-01-11 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vitro
US20090269803A1 (en) * 2005-09-21 2009-10-29 Andreas Meyer In Vivo Generation of Dna, Rna, Peptide, and Protein Libraries
KR101098032B1 (ko) * 2008-04-22 2011-12-23 한국생명공학연구원 4차 연속 피씨알, 4차 블록 피씨알, 또는 유전자 합성방법을 이용한 균주 특이적 바코드를 포함하는 유전자 적중 이형접합체 분열효모 균주의 제조방법
CN101532181B (zh) * 2009-03-13 2011-07-20 南京仙奕基因科技有限公司 原位构建基因突变文库的方法及试剂盒
SG11201506696TA (en) 2013-02-26 2015-09-29 Axiomx Inc Methods for the production of libraries for directed evolution
CN110358786B (zh) * 2018-03-07 2022-02-01 江南大学 一种酶突变体
CN111574633B (zh) * 2020-05-15 2022-04-08 清华大学 一种具有广谱抗病毒功能的蛋白质CbAE的应用
CN114921440B (zh) * 2022-05-16 2023-05-05 北京昕大洋科技发展有限公司 一种木聚糖酶突变体及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2641793B1 (fr) * 1988-12-26 1993-10-01 Setratech Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases

Also Published As

Publication number Publication date
CN101070537A (zh) 2007-11-14
CN1210557A (zh) 1999-03-10
DE69733059D1 (de) 2005-05-25
DK0873398T3 (da) 2005-08-15
WO1997025410A1 (en) 1997-07-17
AU1367897A (en) 1997-08-01
EP0873398A1 (de) 1998-10-28
DE69733059T2 (de) 2006-03-02
EP0873398B1 (de) 2005-04-20
JP2000502568A (ja) 2000-03-07

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