CN101532181B - 原位构建基因突变文库的方法及试剂盒 - Google Patents
原位构建基因突变文库的方法及试剂盒 Download PDFInfo
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- CN101532181B CN101532181B CN2009100259250A CN200910025925A CN101532181B CN 101532181 B CN101532181 B CN 101532181B CN 2009100259250 A CN2009100259250 A CN 2009100259250A CN 200910025925 A CN200910025925 A CN 200910025925A CN 101532181 B CN101532181 B CN 101532181B
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- gene
- mutation library
- gene mutation
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- expression vector
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (7)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2009100259250A CN101532181B (zh) | 2009-03-13 | 2009-03-13 | 原位构建基因突变文库的方法及试剂盒 |
PCT/US2010/027364 WO2010105276A1 (en) | 2009-03-13 | 2010-03-15 | Method for constructing mutagenesis libraries in situ |
US13/254,859 US9416359B2 (en) | 2009-03-13 | 2010-03-15 | Method for constructing mutagenesis libraries in situ |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2009100259250A CN101532181B (zh) | 2009-03-13 | 2009-03-13 | 原位构建基因突变文库的方法及试剂盒 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101532181A CN101532181A (zh) | 2009-09-16 |
CN101532181B true CN101532181B (zh) | 2011-07-20 |
Family
ID=41103036
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2009100259250A Expired - Fee Related CN101532181B (zh) | 2009-03-13 | 2009-03-13 | 原位构建基因突变文库的方法及试剂盒 |
Country Status (3)
Country | Link |
---|---|
US (1) | US9416359B2 (zh) |
CN (1) | CN101532181B (zh) |
WO (1) | WO2010105276A1 (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111379032B (zh) * | 2018-12-28 | 2024-07-23 | 北京贝瑞和康生物技术有限公司 | 一种用于构建同时实现基因组拷贝数变异检测和基因突变检测的测序文库的方法和试剂盒 |
CN110982807B (zh) * | 2019-12-17 | 2022-04-29 | 云南农业大学 | 一种高效稳定的纤维素酶突变体 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1210557A (zh) * | 1996-01-10 | 1999-03-10 | 诺沃挪第克公司 | 用于在细胞中体内产生突变体文库方法 |
US20040023327A1 (en) * | 1995-12-07 | 2004-02-05 | Short Jay M. | End selection in directed evolution |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030044980A1 (en) * | 2001-06-05 | 2003-03-06 | Gorilla Genomics, Inc. | Methods for low background cloning of DNA using long oligonucleotides |
US20050266417A1 (en) * | 2003-09-12 | 2005-12-01 | Francis Barany | Methods for identifying target nucleic acid molecules |
WO2005080603A2 (en) * | 2003-12-17 | 2005-09-01 | Arizona Board Of Regents, A Body Corporate Acting On Behalf Of Arizona State University | Single molecule detection using molecular motors |
GB2461706A (en) * | 2008-07-08 | 2010-01-13 | Univ Dublin | Pseudomonas putida styrene monooxygenase variants |
-
2009
- 2009-03-13 CN CN2009100259250A patent/CN101532181B/zh not_active Expired - Fee Related
-
2010
- 2010-03-15 US US13/254,859 patent/US9416359B2/en not_active Expired - Fee Related
- 2010-03-15 WO PCT/US2010/027364 patent/WO2010105276A1/en active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040023327A1 (en) * | 1995-12-07 | 2004-02-05 | Short Jay M. | End selection in directed evolution |
CN1210557A (zh) * | 1996-01-10 | 1999-03-10 | 诺沃挪第克公司 | 用于在细胞中体内产生突变体文库方法 |
Non-Patent Citations (3)
Title |
---|
HIROTO OKAYAMA AND PAUL BERG.High-Efficiency Cloning of Full-Length cDNA.《MOLECULAR AND CELLULAR BIOLOGY》.1982,第2卷(第2期),161-170. * |
Kentaro Miyazaki.Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole plasmid(MEGAWHOP).《Methods in Molecular Biology》.2003,第231卷(第1期), * |
贾向东等.几种定向进化技术的比较及文库构建策略.《中国生物工程杂志》.2003,第23卷(第12期),68-72. * |
Also Published As
Publication number | Publication date |
---|---|
US9416359B2 (en) | 2016-08-16 |
WO2010105276A1 (en) | 2010-09-16 |
CN101532181A (zh) | 2009-09-16 |
US20120004142A1 (en) | 2012-01-05 |
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Owner name: NANJING XIANYI GENE TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: NANJING NORMAL UNIVERSITY Effective date: 20110317 |
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Free format text: CORRECT: ADDRESS; FROM: 210046 NO. 1, WENYUAN ROAD, YADONG NEW AREA, NANJING CITY, JIANGSU PROVINCE TO: 211135 NO. 666, DONGQI ROAD, QILIN TECHNOLOGY VENTURE PARK (ECO-TECHNOLOGY CITY), NANJING CITY, JIANGSU PROVINCE |
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Effective date of registration: 20110317 Address after: 211135 Jiangsu city of Nanjing province kylin Technology Park (eco technology city) Qi East Road No. 666 Applicant after: Nanjing Xianyi Gene Technology Co., Ltd. Address before: Ya Dong New District Yuen Road 210046 in Jiangsu province Nanjing City No. 1 Applicant before: Nanjing Normal University |
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Owner name: SHINE-E BIOTECH (NANJING) CO., LTD. Free format text: FORMER OWNER: NANJING XIANYI GENE TECHNOLOGY CO., LTD. Effective date: 20121116 |
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Effective date of registration: 20121116 Address after: 210000 No. 9 Peng Shan Road, University Science Park, Nanjing, Jiangsu, Jiangning Patentee after: Xian Yi Biotechnology (Nanjing) Co., Ltd. Address before: 211135 Jiangsu city of Nanjing province kylin Technology Park (eco technology city) Qi East Road No. 666 Patentee before: Nanjing Xianyi Gene Technology Co., Ltd. |
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