MX2021003847A - Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual. - Google Patents

Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual.

Info

Publication number
MX2021003847A
MX2021003847A MX2021003847A MX2021003847A MX2021003847A MX 2021003847 A MX2021003847 A MX 2021003847A MX 2021003847 A MX2021003847 A MX 2021003847A MX 2021003847 A MX2021003847 A MX 2021003847A MX 2021003847 A MX2021003847 A MX 2021003847A
Authority
MX
Mexico
Prior art keywords
cell
sequencing
sample
biological sample
free dna
Prior art date
Application number
MX2021003847A
Other languages
English (en)
Inventor
Patrick A Arensdorf
Samuel Levy
Damek Spacek
Christopher E Ellison
Original Assignee
Bluestar Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bluestar Genomics Inc filed Critical Bluestar Genomics Inc
Publication of MX2021003847A publication Critical patent/MX2021003847A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invención proporciona un método para el análisis de una muestra biológica para determinar múltiples tipos de información de la misma en un flujo de trabajo combinado, simple, donde toda la información se obtiene en un análisis basado en secuenciación. La información incluye la presencia y concentración de proteínas plasmáticas específicas en una muestra de sangre; el número, ubicación, y tipos de modificaciones de histona asociadas con el ADN libre de célula obtenido de la misma muestra; la secuencia de ARNcf y ADNcf en la muestra de ADN libre de célula; e información epigenética que se refiere al ADN libre de célula, tal como perfile de hidroximetilación y metilación, es decir, la distribución de los residuos de 5-hidroximetilcitosina (5hmC) y 5-metilcitosina (5mC), respectivamente. La invención se refiere adicionalmente a un método basado en secuenciación clásica para analizar una muestra biológica para determinar una o más características de secuencia no clásicas de la muestra. También se proporcionan composiciones, kits, y métodos relacionados, que incluyen una modalidad en la cual los adaptadores de secuenciación truncados se utilizan en conjunto con los cebadores de PCR marcados con códigos de barras.
MX2021003847A 2018-10-04 2019-10-03 Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual. MX2021003847A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862741473P 2018-10-04 2018-10-04
PCT/US2019/054582 WO2020072829A2 (en) 2018-10-04 2019-10-03 Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample

Publications (1)

Publication Number Publication Date
MX2021003847A true MX2021003847A (es) 2021-05-27

Family

ID=68296828

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021003847A MX2021003847A (es) 2018-10-04 2019-10-03 Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual.

Country Status (9)

Country Link
US (1) US20210380971A1 (es)
EP (1) EP3861132A2 (es)
JP (1) JP2022504078A (es)
CN (1) CN113166796A (es)
AU (1) AU2019354789A1 (es)
CA (1) CA3114606A1 (es)
MX (1) MX2021003847A (es)
SG (1) SG11202102954QA (es)
WO (1) WO2020072829A2 (es)

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US20240301466A1 (en) * 2021-07-15 2024-09-12 University Of Washington Efficient duplex sequencing using high fidelity next generation sequencing reads
US20230086611A1 (en) * 2021-09-23 2023-03-23 Genomic Testing Cooperative, LCA Compositions and Methods for Targeted NGS Sequencing of cfRNA and cfTNA
JP2024534509A (ja) * 2021-09-23 2024-09-20 ジノミク テスティング コーポレーティブ，エルシーエー ｃｆＲＮＡおよびｃｆＴＮＡの標的ＮＧＳシーケンシングのための組成物および方法
WO2023097295A1 (en) * 2021-11-24 2023-06-01 Alida Biosciences, Inc. Rna and dna analysis using engineered surfaces
WO2024112948A1 (en) * 2022-11-23 2024-05-30 Alida Biosciences, Inc. Chromatin profiling compositions and methods
CN116403645B (zh) * 2023-03-03 2024-01-09 阿里巴巴（中国）有限公司 转录因子结合位点的预测方法及装置

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WO2011127136A1 (en) 2010-04-06 2011-10-13 University Of Chicago Composition and methods related to modification of 5-hydroxymethylcytosine (5-hmc)
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Also Published As

Publication number Publication date
EP3861132A2 (en) 2021-08-11
SG11202102954QA (en) 2021-04-29
AU2019354789A1 (en) 2021-05-27
WO2020072829A3 (en) 2020-08-13
CN113166796A (zh) 2021-07-23
WO2020072829A2 (en) 2020-04-09
JP2022504078A (ja) 2022-01-13
US20210380971A1 (en) 2021-12-09
CA3114606A1 (en) 2020-04-09

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