SG11202102954QA - Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample - Google Patents

Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample

Info

Publication number
SG11202102954QA
SG11202102954QA SG11202102954QA SG11202102954QA SG11202102954QA SG 11202102954Q A SG11202102954Q A SG 11202102954QA SG 11202102954Q A SG11202102954Q A SG 11202102954QA SG 11202102954Q A SG11202102954Q A SG 11202102954QA SG 11202102954Q A SG11202102954Q A SG 11202102954QA
Authority
SG
Singapore
Prior art keywords
nucleosomes
sequencing
proteins
simultaneous
cell
Prior art date
Application number
SG11202102954QA
Inventor
Patrick A Arensdorf
Damek Spacek
Christopher E Ellison
Samuel Levy
Original Assignee
Bluestar Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bluestar Genomics Inc filed Critical Bluestar Genomics Inc
Publication of SG11202102954QA publication Critical patent/SG11202102954QA/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
SG11202102954QA 2018-10-04 2019-10-03 Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample SG11202102954QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862741473P 2018-10-04 2018-10-04
PCT/US2019/054582 WO2020072829A2 (en) 2018-10-04 2019-10-03 Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample

Publications (1)

Publication Number Publication Date
SG11202102954QA true SG11202102954QA (en) 2021-04-29

Family

ID=68296828

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202102954QA SG11202102954QA (en) 2018-10-04 2019-10-03 Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample

Country Status (9)

Country Link
US (1) US20210380971A1 (en)
EP (1) EP3861132A2 (en)
JP (1) JP2022504078A (en)
CN (1) CN113166796A (en)
AU (1) AU2019354789A1 (en)
CA (1) CA3114606A1 (en)
MX (1) MX2021003847A (en)
SG (1) SG11202102954QA (en)
WO (1) WO2020072829A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113517026B (en) * 2021-06-16 2022-08-19 苏州拉索生物芯片科技有限公司 Method and system for generating label sequence applied to biological product, intelligent terminal and computer readable storage medium
WO2023287876A1 (en) * 2021-07-15 2023-01-19 University Of Washington Efficient duplex sequencing using high fidelity next generation sequencing reads
WO2023097295A1 (en) * 2021-11-24 2023-06-01 Alida Biosciences, Inc. Rna and dna analysis using engineered surfaces
CN116403645B (en) * 2023-03-03 2024-01-09 阿里巴巴(中国)有限公司 Method and device for predicting transcription factor binding site

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US5712126A (en) 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA
US6287825B1 (en) 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
EP1954706B1 (en) 2005-11-30 2012-03-28 Epicentre Technologies Corporation Method using reversibly blocked tagging oligonucleotides
JP2010528608A (en) 2007-06-01 2010-08-26 454 ライフ サイエンシーズ コーポレイション System and method for identifying individual samples from complex mixtures
EP2191011B1 (en) 2007-08-29 2017-03-29 Illumina Cambridge Limited Method for sequencing a polynucleotide template
US9115386B2 (en) 2008-09-26 2015-08-25 Children's Medical Center Corporation Selective oxidation of 5-methylcytosine by TET-family proteins
US20100323348A1 (en) 2009-01-31 2010-12-23 The Regents Of The University Of Colorado, A Body Corporate Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process
EP2464729B9 (en) 2009-08-14 2014-12-31 Epicentre Technologies Corporation METHODS, COMPOSITIONS, AND KITS FOR GENERATING rRNA-DEPLETED SAMPLES OR ISOLATING rRNA FROM SAMPLES
WO2011127136A1 (en) 2010-04-06 2011-10-13 University Of Chicago Composition and methods related to modification of 5-hydroxymethylcytosine (5-hmc)
EP3052658B1 (en) * 2013-09-30 2021-03-31 Vesicode AB Methods to profile molecular complexes by using proximity dependant bar-coding
JP6602294B2 (en) 2013-10-17 2019-11-06 タカラ バイオ ユーエスエー, インコーポレイテッド Methods for adding adapters to nucleic acids and compositions for performing the methods
WO2017075265A1 (en) * 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents
PT3440205T (en) 2016-04-07 2021-08-06 Univ Leland Stanford Junior Noninvasive diagnostics by sequencing 5-hydroxymethylated cell-free dna
US20170298422A1 (en) 2016-04-18 2017-10-19 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous single-molecule epigenetic imaging of dna methylation and hydroxymethylation
EP3497123A1 (en) * 2016-08-12 2019-06-19 CDI Laboratories Inc. Compositions and methods for analyzing nucleic acids associated with an analyte
CN106367485B (en) * 2016-08-29 2019-04-26 厦门艾德生物医药科技股份有限公司 Double label connector groups of a kind of more positioning for detecting gene mutation and its preparation method and application
US20180080021A1 (en) 2016-09-17 2018-03-22 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous sequencing of rna and dna from the same sample

Also Published As

Publication number Publication date
CA3114606A1 (en) 2020-04-09
WO2020072829A2 (en) 2020-04-09
US20210380971A1 (en) 2021-12-09
MX2021003847A (en) 2021-05-27
EP3861132A2 (en) 2021-08-11
JP2022504078A (en) 2022-01-13
AU2019354789A1 (en) 2021-05-27
CN113166796A (en) 2021-07-23
WO2020072829A3 (en) 2020-08-13

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