BR112022009072A2 - Processo de realização de uma reação bioquímica, macromolécula idr, polipeptídeo idr, molécula de ácido nucleico isolada, vetor de expressão de polinucleotídeo recombinante, célula hospedeira, cultura celular, kit, processo para estimular ou melhorar a desmistura líquido-líquido, uso de íons de metais multivalentes, método para determinar a sequência nucleotídica e uso de uma macromolécula idr ou um polipeptídeo idr - Google Patents

Processo de realização de uma reação bioquímica, macromolécula idr, polipeptídeo idr, molécula de ácido nucleico isolada, vetor de expressão de polinucleotídeo recombinante, célula hospedeira, cultura celular, kit, processo para estimular ou melhorar a desmistura líquido-líquido, uso de íons de metais multivalentes, método para determinar a sequência nucleotídica e uso de uma macromolécula idr ou um polipeptídeo idr

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Publication number
BR112022009072A2
BR112022009072A2 BR112022009072A BR112022009072A BR112022009072A2 BR 112022009072 A2 BR112022009072 A2 BR 112022009072A2 BR 112022009072 A BR112022009072 A BR 112022009072A BR 112022009072 A BR112022009072 A BR 112022009072A BR 112022009072 A2 BR112022009072 A2 BR 112022009072A2
Authority
BR
Brazil
Prior art keywords
idr
liquid
polypeptide
macromolecule
macromolecules
Prior art date
Application number
BR112022009072A
Other languages
English (en)
Inventor
Armes Niall
Williams Hannah
forrest Matthew
PARKER Mathew
Liu Sidong
Parker Lauren
Original Assignee
Biocrucible Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocrucible Ltd filed Critical Biocrucible Ltd
Publication of BR112022009072A2 publication Critical patent/BR112022009072A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

Abstract

PROCESSO DE REALIZAÇÃO DE UMA REAÇÃO BIOQUÍMICA, MACROMOLÉCULA IDR, POLIPEPTÍDEO IDR, MOLÉCULA DE ÁCIDO NUCLEICO ISOLADA, VETOR DE EXPRESSÃO DE POLINUCLEOTÍDEO RECOMBINANTE, CÉLULA HOSPEDEIRA, CULTURA CELULAR, KIT, PROCESSO PARA ESTIMULAR OU MELHORAR A DESMISTURA LÍQUIDOLÍQUIDO, USO DE ÍONS DE METAIS MULTIVALENTES, MÉTODO PARA DETERMINAR A SEQUÊNCIA NUCLEOTÍDICA E USO DE UMA MACROMOLÉCULA IDR OU UM POLIPEPTÍDEO IDR. A invenção refere-se a processos para realizar reações bioquímicas, como em um sistema de reação aquoso in vitro. Os processos envolvem macromoléculas, particularmente polipeptídeos, compreendendo uma ou mais regiões intrinsecamente desordenadas funcionais (IDRs). A invenção também se refere a macromoléculas IDR, incluindo polipeptídeos IDR, incluindo macromoléculas ou polipeptídeos compreendendo uma sequência de aminoácidos marcada que compreende ou consiste em um ou mais IDRs funcionais. Tais IDRs funcionais são capazes de aumentar a eficiência da reação bioquímica. A invenção refere-se a kits compreendendo quaisquer dessas macromoléculas e polipeptídeos. A invenção refere-se ainda a processos para estimular ou intensificar a desmistura líquido-líquido em uma solução, usando quaisquer dessas macromoléculas e polipeptídeos, inclusive em combinação com íons de metais multivalentes, fornecendo assim reagentes capazes de aumentar a eficiência de uma reação bioquímica.
BR112022009072A 2019-11-11 2020-11-11 Processo de realização de uma reação bioquímica, macromolécula idr, polipeptídeo idr, molécula de ácido nucleico isolada, vetor de expressão de polinucleotídeo recombinante, célula hospedeira, cultura celular, kit, processo para estimular ou melhorar a desmistura líquido-líquido, uso de íons de metais multivalentes, método para determinar a sequência nucleotídica e uso de uma macromolécula idr ou um polipeptídeo idr BR112022009072A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1916379.9A GB201916379D0 (en) 2019-11-11 2019-11-11 Biochemical reaction methods and reagents
PCT/GB2020/052866 WO2021094746A1 (en) 2019-11-11 2020-11-11 Biochemical reaction methods and reagents comprising intrinsically disordered regions

Publications (1)

Publication Number Publication Date
BR112022009072A2 true BR112022009072A2 (pt) 2022-07-26

Family

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Family Applications (1)

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BR112022009072A BR112022009072A2 (pt) 2019-11-11 2020-11-11 Processo de realização de uma reação bioquímica, macromolécula idr, polipeptídeo idr, molécula de ácido nucleico isolada, vetor de expressão de polinucleotídeo recombinante, célula hospedeira, cultura celular, kit, processo para estimular ou melhorar a desmistura líquido-líquido, uso de íons de metais multivalentes, método para determinar a sequência nucleotídica e uso de uma macromolécula idr ou um polipeptídeo idr

Country Status (12)

Country Link
US (2) US20220112547A1 (pt)
EP (1) EP4058599A1 (pt)
JP (1) JP2023501002A (pt)
KR (1) KR20220113948A (pt)
CN (1) CN114945682A (pt)
AU (1) AU2020382121A1 (pt)
BR (1) BR112022009072A2 (pt)
CA (1) CA3157435A1 (pt)
GB (1) GB201916379D0 (pt)
IL (1) IL292885A (pt)
MX (1) MX2022005677A (pt)
WO (1) WO2021094746A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108998406B (zh) * 2018-08-03 2022-05-10 福州大学 一种人类原代培养细胞基因组编辑、定点基因敲入方法
GB201916379D0 (en) * 2019-11-11 2019-12-25 Biocrucible Ltd Biochemical reaction methods and reagents
CN113355389B (zh) * 2020-03-05 2022-11-15 广西扬翔股份有限公司 利用CRISPR/Cas12a系统靶向富集核酸目标区域的方法及其应用
US20240110221A1 (en) * 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics

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Publication number Priority date Publication date Assignee Title
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
ATE400663T1 (de) 2002-02-21 2008-07-15 Asm Scient Inc Rekombinase-polymerase-amplifikation
CN1942761A (zh) 2004-04-21 2007-04-04 东丽株式会社 芯片实验室用基板
DE102005052752A1 (de) 2005-11-04 2007-05-10 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
EP1929049B1 (en) 2005-07-25 2013-04-10 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
US8071308B2 (en) * 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
EP2089159B1 (en) 2007-05-03 2013-01-23 Clondiag GmbH Assays
US8560130B2 (en) 2007-12-04 2013-10-15 Lester F. Ludwig Software controlled lab-on-a-chip emulation
CN102026725B (zh) 2008-03-14 2014-10-29 科隆迪亚戈有限公司 一种用于检测样品中分析物的装置及其方法
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
US10533167B2 (en) 2016-06-09 2020-01-14 The Trustees Of Princeton University Optogenetic tool for rapid and reversible clustering of proteins
GB201916379D0 (en) * 2019-11-11 2019-12-25 Biocrucible Ltd Biochemical reaction methods and reagents

Also Published As

Publication number Publication date
US20220145378A1 (en) 2022-05-12
US11807899B2 (en) 2023-11-07
EP4058599A1 (en) 2022-09-21
MX2022005677A (es) 2022-09-07
CA3157435A1 (en) 2021-05-20
AU2020382121A1 (en) 2022-06-16
CN114945682A (zh) 2022-08-26
WO2021094746A1 (en) 2021-05-20
KR20220113948A (ko) 2022-08-17
US20220112547A1 (en) 2022-04-14
GB201916379D0 (en) 2019-12-25
JP2023501002A (ja) 2023-01-17
IL292885A (en) 2022-07-01
AU2020382121A8 (en) 2022-07-28

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