MX2022005677A - Procedimientos y reactivos de reaccion bioquimica que comprenden regiones intrinsecamente desordenadas. - Google Patents

Procedimientos y reactivos de reaccion bioquimica que comprenden regiones intrinsecamente desordenadas.

Info

Publication number
MX2022005677A
MX2022005677A MX2022005677A MX2022005677A MX2022005677A MX 2022005677 A MX2022005677 A MX 2022005677A MX 2022005677 A MX2022005677 A MX 2022005677A MX 2022005677 A MX2022005677 A MX 2022005677A MX 2022005677 A MX2022005677 A MX 2022005677A
Authority
MX
Mexico
Prior art keywords
macromolecules
polypeptides
biochemical reaction
relates
idrs
Prior art date
Application number
MX2022005677A
Other languages
English (en)
Inventor
Niall Armes
Hannah Williams
Matthew Forrest
Mathew Parker
Sidong Liu
Lauren Parker
Original Assignee
Biocrucible Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocrucible Ltd filed Critical Biocrucible Ltd
Publication of MX2022005677A publication Critical patent/MX2022005677A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer

Abstract

La invención se refiere a procedimientos para realizar reacciones bioquímicas, tal como en un sistema de reacción in vitro acuoso. Los procedimientos implican macromoléculas, particularmente polipéptidos, que comprenden una o más regiones funcionales intrínsecamente desordenadas (RID). La invención también se refiere a macromoléculas -RDI, incluyendo polipéptidos-RID, incluyendo macromoléculas o polipéptidos que comprenden una secuencia de aminoácidos marcada que comprende o consiste en una o más RID funcionales. Dichas RID funcionales son capaces de aumentar la eficiencia de la reacción bioquímica. La invención se refiere a kits que comprenden cualquiera de dichas macromoléculas y polipéptidos. La invención se refiere además a procedimientos para estimular o mejorar la separación líquido-líquido en una solución usando cualquiera de tales macromoléculas y polipéptidos, incluso en combinación con iones metálicos multivalentes, proporcionando así reactivos capaces de aumentar la eficiencia de una reacción bioquímica.
MX2022005677A 2019-11-11 2020-11-11 Procedimientos y reactivos de reaccion bioquimica que comprenden regiones intrinsecamente desordenadas. MX2022005677A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1916379.9A GB201916379D0 (en) 2019-11-11 2019-11-11 Biochemical reaction methods and reagents
PCT/GB2020/052866 WO2021094746A1 (en) 2019-11-11 2020-11-11 Biochemical reaction methods and reagents comprising intrinsically disordered regions

Publications (1)

Publication Number Publication Date
MX2022005677A true MX2022005677A (es) 2022-09-07

Family

ID=69062276

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022005677A MX2022005677A (es) 2019-11-11 2020-11-11 Procedimientos y reactivos de reaccion bioquimica que comprenden regiones intrinsecamente desordenadas.

Country Status (12)

Country Link
US (2) US20220112547A1 (es)
EP (1) EP4058599A1 (es)
JP (1) JP2023501002A (es)
KR (1) KR20220113948A (es)
CN (1) CN114945682A (es)
AU (1) AU2020382121A1 (es)
BR (1) BR112022009072A2 (es)
CA (1) CA3157435A1 (es)
GB (1) GB201916379D0 (es)
IL (1) IL292885A (es)
MX (1) MX2022005677A (es)
WO (1) WO2021094746A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108998406B (zh) * 2018-08-03 2022-05-10 福州大学 一种人类原代培养细胞基因组编辑、定点基因敲入方法
GB201916379D0 (en) * 2019-11-11 2019-12-25 Biocrucible Ltd Biochemical reaction methods and reagents
CN113355389B (zh) * 2020-03-05 2022-11-15 广西扬翔股份有限公司 利用CRISPR/Cas12a系统靶向富集核酸目标区域的方法及其应用
US20240110221A1 (en) * 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
ATE400663T1 (de) 2002-02-21 2008-07-15 Asm Scient Inc Rekombinase-polymerase-amplifikation
CN1942761A (zh) 2004-04-21 2007-04-04 东丽株式会社 芯片实验室用基板
DE102005052752A1 (de) 2005-11-04 2007-05-10 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
EP1929049B1 (en) 2005-07-25 2013-04-10 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
US8071308B2 (en) * 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
EP2089159B1 (en) 2007-05-03 2013-01-23 Clondiag GmbH Assays
US8560130B2 (en) 2007-12-04 2013-10-15 Lester F. Ludwig Software controlled lab-on-a-chip emulation
CN102026725B (zh) 2008-03-14 2014-10-29 科隆迪亚戈有限公司 一种用于检测样品中分析物的装置及其方法
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
US10533167B2 (en) 2016-06-09 2020-01-14 The Trustees Of Princeton University Optogenetic tool for rapid and reversible clustering of proteins
GB201916379D0 (en) * 2019-11-11 2019-12-25 Biocrucible Ltd Biochemical reaction methods and reagents

Also Published As

Publication number Publication date
US20220145378A1 (en) 2022-05-12
US11807899B2 (en) 2023-11-07
EP4058599A1 (en) 2022-09-21
CA3157435A1 (en) 2021-05-20
BR112022009072A2 (pt) 2022-07-26
AU2020382121A1 (en) 2022-06-16
CN114945682A (zh) 2022-08-26
WO2021094746A1 (en) 2021-05-20
KR20220113948A (ko) 2022-08-17
US20220112547A1 (en) 2022-04-14
GB201916379D0 (en) 2019-12-25
JP2023501002A (ja) 2023-01-17
IL292885A (en) 2022-07-01
AU2020382121A8 (en) 2022-07-28

Similar Documents

Publication Publication Date Title
MX2022005677A (es) Procedimientos y reactivos de reaccion bioquimica que comprenden regiones intrinsecamente desordenadas.
Kawakami et al. Diverse backbone-cyclized peptides via codon reprogramming
EP4265723A3 (en) Reagents and methods for molecular barcoding of nucleic acids of single cells
CY1124717T1 (el) Μεθοδοι, kit και συσκευη για επεκταση πληθυσμου κυτταρων
BR112018010681A8 (pt) método para modificar um sítio alvejado, complexo de enzima de modificação de ácido nucleico, e, ácido nucleico.
AU2024202391A1 (en) Systems and methods for performing biological assays
GB2513793A (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
MX2021003771A (es) Analisis de analitos multiples usando un ensayo unico.
BRPI0812878C1 (pt) anticorpo isolado ou parte funcional do mesmo, vetor, método para produzir um anticorpo ou uma parte funcional do mesmo, para produzir uma célula produtora de anticorpo e para produzir um anticorpo capaz de se ligar especificamente a rsv, composição, e, uso de um anticorpo ou parte funcional do mesmo ou de uma composição ou de vetor
WO2019135816A3 (en) Novel nucleic acid modifiers
AU2018234832A1 (en) CRISPR effector system based diagnostics for virus detection
WO2016130704A3 (en) Methods and compositions for analyzing cellular components
BR112018076259A2 (pt) tagmentação usando transposomas imobilizados com ligantes
WO2016033251A3 (en) Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
EP4234718A3 (en) Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
WO2016145416A3 (en) Proteomic analysis with nucleic acid identifiers
WO2014145820A3 (en) Multiple tagging of long dna fragments
SA516371797B1 (ar) تركيبة وطريقة لثبات الأحماض النووية في عينات بيولوجية
EP3845640A3 (en) Modified tranposases for improved insertion sequence bias and increased dna input tolerence
WO2009076485A3 (en) Sequencing of nucleic acids
ATE419388T1 (de) Nukleinsäureanreicherung
CL2022002379A1 (es) Molécula de ácido nucleico interferente corto (sina); composición que la comprende; y su uso.
WO2018053365A8 (en) METHODS OF PREPARING NUCLEIC ACID SAMPLE FOR ANALYSIS OF ACELLULAR DNA
BR112014027239A2 (pt) peptídeo, método de identificação de peptídeos, método para o tratamento de câncer, nucleotídeo, vetor, uso de um peptídeo e invenção
SG11202011050TA (en) Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications