BR112018010681A8 - método para modificar um sítio alvejado, complexo de enzima de modificação de ácido nucleico, e, ácido nucleico. - Google Patents

método para modificar um sítio alvejado, complexo de enzima de modificação de ácido nucleico, e, ácido nucleico.

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Publication number
BR112018010681A8
BR112018010681A8 BR112018010681A BR112018010681A BR112018010681A8 BR 112018010681 A8 BR112018010681 A8 BR 112018010681A8 BR 112018010681 A BR112018010681 A BR 112018010681A BR 112018010681 A BR112018010681 A BR 112018010681A BR 112018010681 A8 BR112018010681 A8 BR 112018010681A8
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Brazil
Prior art keywords
nucleic acid
stranded dna
double stranded
targeted site
complex
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BR112018010681A
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English (en)
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BR112018010681A2 (pt
BR112018010681B1 (pt
Inventor
Kondo Akihiko
Nishida Keiji
Shimatani Zenpei
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Univ Kobe Nat Univ Corp
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Publication of BR112018010681A2 publication Critical patent/BR112018010681A2/pt
Publication of BR112018010681A8 publication Critical patent/BR112018010681A8/pt
Publication of BR112018010681B1 publication Critical patent/BR112018010681B1/pt

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Abstract

a presente invenção provê um método para modificar um sítio alvejado em dna de duplo filamento possuído por células de plantas monocotiledôneas, que inclui uma etapa para, ao trazer um complexo, no qual um módulo de reconhecimento de sequência de ácido nucleico que se liga especificamente a uma sequência de nucleotídeos alvos em um dna de duplo filamento selecionado é ligado a uma enzima conversora de base de ácido nucleico, em contato com o dna de duplo filamento, convertendo um ou mais nucleotídeos no sítio alvejado para um ou mais nucleotídeos diferentes, deletando um ou mais nucleotídeos no sítio alvejado, ou inserindo um ou mais nucleotídeos no sítio alvejado, sem clivar pelo menos um dos filamentos do dna de duplo filamento no sítio alvejado, em que o contato entre o dna de duplo filamento e o complexo é alcançado ao introduzir uma codificação de ácido nucleico para o complexo às células de plantas monocotiledôneas. além disso, é provido também o complexo a ser usado neste método, no qual o módulo de reconhecimento de sequência de ácido nucleico que se liga especificamente a uma sequência de nucleotídeos alvos em um dna de duplo filamento possuído por células de plantas monocotiledôneas é ligado a uma enzima conversora de base de ácido nucleico.
BR112018010681-0A 2015-11-27 2016-11-25 Método para modificar um sítio alvejado, complexo de enzima de modificação de ácido nucleico, e, ácido nucleico BR112018010681B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2015-232379 2015-11-27
JP2015232379 2015-11-27
JP2016-134613 2016-07-06
JP2016134613 2016-07-06
PCT/JP2016/085075 WO2017090761A1 (ja) 2015-11-27 2016-11-25 標的化したｄｎａ配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体

Publications (3)

Publication Number Publication Date
BR112018010681A2 BR112018010681A2 (pt) 2018-11-13
BR112018010681A8 true BR112018010681A8 (pt) 2019-02-26
BR112018010681B1 BR112018010681B1 (pt) 2024-09-17

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Also Published As

Publication number Publication date
EP3382019A1 (en) 2018-10-03
US11220693B2 (en) 2022-01-11
EP3382019B1 (en) 2022-05-04
KR20180081811A (ko) 2018-07-17
BR112018010681A2 (pt) 2018-11-13
JP6923205B2 (ja) 2021-08-18
HK1256888A1 (zh) 2019-10-04
CN108495932A (zh) 2018-09-04
JPWO2017090761A1 (ja) 2018-10-25
WO2017090761A1 (ja) 2017-06-01
DK3382019T3 (da) 2022-05-30
ES2914623T3 (es) 2022-06-14
CN108495932B (zh) 2022-08-09
KR102061438B1 (ko) 2019-12-31
EP3382019A4 (en) 2019-04-24
US20190085342A1 (en) 2019-03-21

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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