BR112018010681A2 - método para modificar um sítio alvejado, complexo de enzima de modificação de ácido nucleico, e, ácido nucleico. - Google Patents

método para modificar um sítio alvejado, complexo de enzima de modificação de ácido nucleico, e, ácido nucleico.

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Publication number
BR112018010681A2
BR112018010681A2 BR112018010681-0A BR112018010681A BR112018010681A2 BR 112018010681 A2 BR112018010681 A2 BR 112018010681A2 BR 112018010681 A BR112018010681 A BR 112018010681A BR 112018010681 A2 BR112018010681 A2 BR 112018010681A2
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Prior art keywords
nucleic acid
targeted site
stranded dna
double
nucleotides
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BR112018010681-0A
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English (en)
Japanese (ja)
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BR112018010681A8 (pt
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Nishida Keiji
Shimatani Zenpei
Kondo Akihiko
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National University Corporation Kobe University
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Publication of BR112018010681A2 publication Critical patent/BR112018010681A2/pt
Publication of BR112018010681A8 publication Critical patent/BR112018010681A8/pt

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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N9/14—Hydrolases (3)
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    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)

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Abstract

a presente invenção provê um método para modificar um sítio alvejado em dna de duplo filamento possuído por células de plantas monocotiledôneas, que inclui uma etapa para, ao trazer um complexo, no qual um módulo de reconhecimento de sequência de ácido nucleico que se liga especificamente a uma sequência de nucleotídeos alvos em um dna de duplo filamento selecionado é ligado a uma enzima conversora de base de ácido nucleico, em contato com o dna de duplo filamento, convertendo um ou mais nucleotídeos no sítio alvejado para um ou mais nucleotídeos diferentes, deletando um ou mais nucleotídeos no sítio alvejado, ou inserindo um ou mais nucleotídeos no sítio alvejado, sem clivar pelo menos um dos filamentos do dna de duplo filamento no sítio alvejado, em que o contato entre o dna de duplo filamento e o complexo é alcançado ao introduzir uma codificação de ácido nucleico para o complexo às células de plantas monocotiledôneas. além disso, é provido também o complexo a ser usado neste método, no qual o módulo de reconhecimento de sequência de ácido nucleico que se liga especificamente a uma sequência de nucleotídeos alvos em um dna de duplo filamento possuído por células de plantas monocotiledôneas é ligado a uma enzima conversora de base de ácido nucleico.
BR112018010681A 2015-11-27 2016-11-25 método para modificar um sítio alvejado, complexo de enzima de modificação de ácido nucleico, e, ácido nucleico. BR112018010681A8 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2015-232379 2015-11-27
JP2015232379 2015-11-27
JP2016-134613 2016-07-06
JP2016134613 2016-07-06
PCT/JP2016/085075 WO2017090761A1 (ja) 2015-11-27 2016-11-25 標的化したｄｎａ配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体

Publications (2)

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BR112018010681A2 true BR112018010681A2 (pt) 2018-11-13
BR112018010681A8 BR112018010681A8 (pt) 2019-02-26

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US (1) US11220693B2 (pt)
EP (1) EP3382019B1 (pt)
JP (1) JP6923205B2 (pt)
KR (1) KR102061438B1 (pt)
CN (1) CN108495932B (pt)
BR (1) BR112018010681A8 (pt)
DK (1) DK3382019T3 (pt)
ES (1) ES2914623T3 (pt)
HK (1) HK1256888A1 (pt)
WO (1) WO2017090761A1 (pt)

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WO2017090761A1 (ja) 2015-11-27 2017-06-01 国立大学法人神戸大学 標的化したｄｎａ配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体
CA3021281C (en) 2016-04-21 2021-07-27 National University Corporation Kobe University Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor

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EP3382019A1 (en) 2018-10-03
HK1256888A1 (zh) 2019-10-04
US20190085342A1 (en) 2019-03-21
KR20180081811A (ko) 2018-07-17
JPWO2017090761A1 (ja) 2018-10-25
BR112018010681A8 (pt) 2019-02-26
KR102061438B1 (ko) 2019-12-31
EP3382019A4 (en) 2019-04-24
ES2914623T3 (es) 2022-06-14
WO2017090761A1 (ja) 2017-06-01
JP6923205B2 (ja) 2021-08-18
CN108495932B (zh) 2022-08-09
US11220693B2 (en) 2022-01-11
DK3382019T3 (da) 2022-05-30
EP3382019B1 (en) 2022-05-04
CN108495932A (zh) 2018-09-04

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