ES2164112T3 - Amplificacion de acidos nucleicos por desplazamiento de hebra isotermico. - Google Patents

Amplificacion de acidos nucleicos por desplazamiento de hebra isotermico.

Info

Publication number
ES2164112T3
ES2164112T3 ES95103868T ES95103868T ES2164112T3 ES 2164112 T3 ES2164112 T3 ES 2164112T3 ES 95103868 T ES95103868 T ES 95103868T ES 95103868 T ES95103868 T ES 95103868T ES 2164112 T3 ES2164112 T3 ES 2164112T3
Authority
ES
Spain
Prior art keywords
amplification
initiators
hebra
isothermal
displacement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95103868T
Other languages
English (en)
Inventor
Nanibhushan Dattagupta
Paul Douglas Stull
Marc Spingola
Daniel Louis Kacian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ES2164112T3 publication Critical patent/ES2164112T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE PRESENTAN METODOS PARA AMPLIFICAR SECUENCIAS DE ACIDO NUCLEICO UTILIZANDO UNA POLIMERASA DE ACIDO NUCLEICO QUE CARECE DE ACTIVIDAD DE EXONUCLEASA 5'' Y UN CONJUNTO DE INICIADORES OLIGONUCLEOTIDOS. PREFERIBLEMENTE, SE UTILIZA UNA MATRIZ INICIADORA. LA MATRIZ INICIADORA CONTIENE DOS CONJUNTOS DE INICIADORES. UN CONJUNTO CONTIENE AL MENOS DOS INICIADORES COMPLEMENTARIOS. EL OTRO CONJUNTO CONTIENE AL MENOS DOS INICIADORES DE SENTIDO. USANDO LOS METODOS DESCRITOS PUEDE LLEVARSE A CABO LA AMPLIFICACION BAJO CONDICIONES MEDIO AMBIENTALES ESENCIALMENTE CONSTANTES SIN EL REQUERIMIENTOS DE LA ACTIVIDAD DE EXONUCLEASA O LA RESTRICCION DE LA ACTIVIDAD DE ENDONUCLEASA.
ES95103868T 1994-03-16 1995-03-16 Amplificacion de acidos nucleicos por desplazamiento de hebra isotermico. Expired - Lifetime ES2164112T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US21508194A 1994-03-16 1994-03-16

Publications (1)

Publication Number Publication Date
ES2164112T3 true ES2164112T3 (es) 2002-02-16

Family

ID=22801567

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95103868T Expired - Lifetime ES2164112T3 (es) 1994-03-16 1995-03-16 Amplificacion de acidos nucleicos por desplazamiento de hebra isotermico.

Country Status (11)

Country Link
US (4) US6087133A (es)
EP (1) EP0676476B1 (es)
JP (1) JPH09510351A (es)
KR (1) KR100230718B1 (es)
AT (1) ATE207543T1 (es)
AU (1) AU687535B2 (es)
CA (1) CA2185239C (es)
DE (1) DE69523360T2 (es)
DK (1) DK0676476T3 (es)
ES (1) ES2164112T3 (es)
WO (1) WO1995025180A1 (es)

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DE69523360T2 (de) 2002-07-11
KR970701791A (ko) 1997-04-12
USRE38960E1 (en) 2006-01-31
US6214587B1 (en) 2001-04-10
JPH09510351A (ja) 1997-10-21
CA2185239A1 (en) 1995-09-21
USRE39007E1 (en) 2006-03-07
US6087133A (en) 2000-07-11
DE69523360D1 (de) 2001-11-29
CA2185239C (en) 2002-12-17
AU2101695A (en) 1995-10-03
KR100230718B1 (ko) 1999-11-15
EP0676476A1 (en) 1995-10-11
EP0676476B1 (en) 2001-10-24
ATE207543T1 (de) 2001-11-15
AU687535B2 (en) 1998-02-26
DK0676476T3 (da) 2001-12-03
WO1995025180A1 (en) 1995-09-21

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