BR112023018152A2 - Preparação de biblioteca genômica e ensaios epigenéticos direcionados com o uso de ribonucleoproteínas de cas-grna - Google Patents
Preparação de biblioteca genômica e ensaios epigenéticos direcionados com o uso de ribonucleoproteínas de cas-grnaInfo
- Publication number
- BR112023018152A2 BR112023018152A2 BR112023018152A BR112023018152A BR112023018152A2 BR 112023018152 A2 BR112023018152 A2 BR 112023018152A2 BR 112023018152 A BR112023018152 A BR 112023018152A BR 112023018152 A BR112023018152 A BR 112023018152A BR 112023018152 A2 BR112023018152 A2 BR 112023018152A2
- Authority
- BR
- Brazil
- Prior art keywords
- cas
- subsequence
- grna
- genomic library
- library preparation
- Prior art date
Links
- 238000007419 epigenetic assay Methods 0.000 title abstract 3
- 102000004389 Ribonucleoproteins Human genes 0.000 title abstract 2
- 108010081734 Ribonucleoproteins Proteins 0.000 title abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 7
- 108091033319 polynucleotide Proteins 0.000 abstract 7
- 239000002157 polynucleotide Substances 0.000 abstract 7
- 239000000203 mixture Substances 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 102000008579 Transposases Human genes 0.000 abstract 1
- 108010020764 Transposases Proteins 0.000 abstract 1
- 108020001507 fusion proteins Proteins 0.000 abstract 1
- 102000037865 fusion proteins Human genes 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
preparação de biblioteca genômica e ensaios epigenéticos direcionados com o uso de ribonucleoproteínas de cas-grna. a presente invenção fornece a preparação de biblioteca genômica com o uso de rnps de cas-grna e ensaios epigenéticos direcionados. algumas composições incluem, de uma primeira espécie, substancialmente apenas os polinucleotídeos de fita simples; de uma segunda espécie, substancialmente apenas polinucleotídeos de fita dupla; e iniciadores de amplificação ligados às extremidades dos segundos polinucleotídeos de fita dupla e substancialmente não ligados a quaisquer extremidades dos primeiros polinucleotídeos de fita dupla. algumas composições incluem uma primeira e uma segunda moléculas de um polinucleotídeo alvo que tem uma sequência, a primeira molécula tendo uma primeira extremidade em uma primeira subsequência, a segunda molécula tendo uma primeira extremidade em uma segunda subsequência, em que a primeira subsequência se sobrepõe apenas parcialmente à segunda subsequência. alguns exemplos fornecem uma composição que inclui um polinucleotídeo alvo e uma primeira proteína de fusão incluindo uma rnp de cas-grna acoplada a uma transposase que tem um adaptador de amplificação acoplado à mesma. a rnp de cas-grna pode ser hibridizada com uma subsequência no polinucleotídeo alvo.
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163158492P | 2021-03-09 | 2021-03-09 | |
| US202163162775P | 2021-03-18 | 2021-03-18 | |
| US202163163381P | 2021-03-19 | 2021-03-19 | |
| US202163228344P | 2021-08-02 | 2021-08-02 | |
| US202163246879P | 2021-09-22 | 2021-09-22 | |
| US202163295432P | 2021-12-30 | 2021-12-30 | |
| PCT/US2022/019252 WO2022192186A1 (en) | 2021-03-09 | 2022-03-08 | Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112023018152A2 true BR112023018152A2 (pt) | 2023-10-31 |
Family
ID=80937135
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112023018152A BR112023018152A2 (pt) | 2021-03-09 | 2022-03-08 | Preparação de biblioteca genômica e ensaios epigenéticos direcionados com o uso de ribonucleoproteínas de cas-grna |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20240287504A1 (pt) |
| EP (1) | EP4305163A1 (pt) |
| JP (1) | JP2024510206A (pt) |
| KR (1) | KR20230154078A (pt) |
| AU (1) | AU2022234741A1 (pt) |
| BR (1) | BR112023018152A2 (pt) |
| CA (1) | CA3209074A1 (pt) |
| IL (1) | IL305444B1 (pt) |
| MX (1) | MX2023010650A (pt) |
| WO (1) | WO2022192186A1 (pt) |
| ZA (1) | ZA202308325B (pt) |
Family Cites Families (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5614389A (en) | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5834202A (en) | 1992-08-04 | 1998-11-10 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US6261808B1 (en) | 1992-08-04 | 2001-07-17 | Replicon, Inc. | Amplification of nucleic acid molecules via circular replicons |
| WO1994003624A1 (en) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US5593826A (en) | 1993-03-22 | 1997-01-14 | Perkin-Elmer Corporation, Applied Biosystems, Inc. | Enzymatic ligation of 3'amino-substituted oligonucleotides |
| WO1994024143A1 (en) | 1993-04-12 | 1994-10-27 | Northwestern University | Method of forming oligonucleotides |
| US5422252A (en) | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
| CA2122203C (en) | 1993-05-11 | 2001-12-18 | Melinda S. Fraiser | Decontamination of nucleic acid amplification reactions |
| KR100189229B1 (ko) | 1993-07-23 | 1999-06-01 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 | 핵산 증폭을 강화하는 방법 |
| FR2708288B1 (fr) | 1993-07-26 | 1995-09-01 | Bio Merieux | Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé. |
| US5523204A (en) | 1993-12-10 | 1996-06-04 | Becton Dickinson And Company | Detection of nucleic acids in cells by strand displacement amplification |
| SE9400522D0 (sv) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| AU687535B2 (en) | 1994-03-16 | 1998-02-26 | Gen-Probe Incorporated | Isothermal strand displacement nucleic acid amplification |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| FR2726277B1 (fr) | 1994-10-28 | 1996-12-27 | Bio Merieux | Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin |
| US5631147A (en) | 1995-09-21 | 1997-05-20 | Becton, Dickinson And Company | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
| US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
| JP2000505312A (ja) | 1996-03-18 | 2000-05-09 | モレキュラー バイオロジー リソーシーズ，インコーポレイテッド | 標的核酸配列増幅 |
| US5773733A (en) | 1996-04-12 | 1998-06-30 | National Science Council | Alumina-aluminum nitride-nickel composites |
| US6124120A (en) | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
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| US6155972A (en) | 1999-02-02 | 2000-12-05 | Acorn Cardiovascular, Inc. | Cardiac constraint jacket construction |
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-
2022
- 2022-03-08 EP EP22712735.4A patent/EP4305163A1/en active Pending
- 2022-03-08 MX MX2023010650A patent/MX2023010650A/es unknown
- 2022-03-08 BR BR112023018152A patent/BR112023018152A2/pt unknown
- 2022-03-08 KR KR1020237034433A patent/KR20230154078A/ko unknown
- 2022-03-08 AU AU2022234741A patent/AU2022234741A1/en active Pending
- 2022-03-08 CA CA3209074A patent/CA3209074A1/en active Pending
- 2022-03-08 WO PCT/US2022/019252 patent/WO2022192186A1/en active Application Filing
- 2022-03-08 US US18/549,344 patent/US20240287504A1/en active Pending
- 2022-03-08 JP JP2023555515A patent/JP2024510206A/ja active Pending
- 2022-03-08 IL IL305444A patent/IL305444B1/en unknown
-
2023
- 2023-08-29 ZA ZA2023/08325A patent/ZA202308325B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2024510206A (ja) | 2024-03-06 |
| CA3209074A1 (en) | 2022-09-15 |
| EP4305163A1 (en) | 2024-01-17 |
| MX2023010650A (es) | 2023-11-28 |
| US20240287504A1 (en) | 2024-08-29 |
| AU2022234741A1 (en) | 2023-09-14 |
| IL305444B1 (en) | 2024-10-01 |
| ZA202308325B (en) | 2024-05-30 |
| WO2022192186A1 (en) | 2022-09-15 |
| IL305444A (en) | 2023-10-01 |
| KR20230154078A (ko) | 2023-11-07 |
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