BR112016007059A2 - Análise de mutação que usa temperatura de fusão e bases universais - Google Patents
Análise de mutação que usa temperatura de fusão e bases universaisInfo
- Publication number
- BR112016007059A2 BR112016007059A2 BR112016007059A BR112016007059A BR112016007059A2 BR 112016007059 A2 BR112016007059 A2 BR 112016007059A2 BR 112016007059 A BR112016007059 A BR 112016007059A BR 112016007059 A BR112016007059 A BR 112016007059A BR 112016007059 A2 BR112016007059 A2 BR 112016007059A2
- Authority
- BR
- Brazil
- Prior art keywords
- probe
- target sequence
- variants
- mutation analysis
- universal bases
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
ANÁLISE DE MUTAÇÃO QUE USA TEMPERATURA DE FUSÃO E BASES UNIVERSAIS. Um método e uma sonda de oligonucleotídeo são descritos para determinar a presença ou ausência de alelos mutantes em um locus genômico. A sonda se liga a diferentes alelos de uma sequência-alvo com temperaturas de fusão (Tm) diferentes. O método determina a Tm da sonda quando a mesma é hibridizada à sequência-alvo para estabelecer se um ácido nucleico variante tal como um alelo mutante está presente ou ausente na sequência-alvo. Pode haver variantes em uma sequência-alvo que não são de interesse, por exemplo, mutações fenotipicamente silenciosas. Para garantir que essas variantes não influenciem a Tm da sonda, a sonda contém sítios de bases universais onde tais variantes sem interesse ocorrem.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB1317355.4A GB201317355D0 (en) | 2013-10-01 | 2013-10-01 | Mutation Analysis |
PCT/GB2014/052939 WO2015049496A1 (en) | 2013-10-01 | 2014-09-30 | Mutation analysis using melting temperature and universal bases |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112016007059A2 true BR112016007059A2 (pt) | 2017-09-19 |
Family
ID=49585117
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112016007059A BR112016007059A2 (pt) | 2013-10-01 | 2014-09-30 | Análise de mutação que usa temperatura de fusão e bases universais |
Country Status (9)
Country | Link |
---|---|
US (1) | US10280452B2 (pt) |
EP (1) | EP3052648B1 (pt) |
JP (1) | JP6461114B2 (pt) |
KR (1) | KR20160057486A (pt) |
CN (1) | CN105916999B (pt) |
BR (1) | BR112016007059A2 (pt) |
GB (1) | GB201317355D0 (pt) |
MX (1) | MX2016004140A (pt) |
WO (1) | WO2015049496A1 (pt) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105368943B (zh) * | 2015-11-21 | 2018-10-16 | 中国人民解放军第三〇九医院 | 一种用于鉴定分枝杆菌菌种的试剂盒及方法 |
CN109868309B (zh) * | 2019-03-05 | 2022-11-11 | 苏州恩可医药科技有限公司 | 基于通用碱基替换插入的单链dna扩增方法 |
CN116391039A (zh) * | 2020-10-30 | 2023-07-04 | 东洋纺株式会社 | 多个靶核酸的同时检测方法 |
CN116287107A (zh) * | 2021-12-21 | 2023-06-23 | 广东菲鹏生物有限公司 | 一种核酸探针及其使用方法 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6511809B2 (en) * | 2000-06-13 | 2003-01-28 | E. I. Du Pont De Nemours And Company | Method for the detection of an analyte by means of a nucleic acid reporter |
US7348146B2 (en) * | 2003-10-02 | 2008-03-25 | Epoch Biosciences, Inc. | Single nucleotide polymorphism analysis of highly polymorphic target sequences |
WO2006095941A1 (en) * | 2005-03-05 | 2006-09-14 | Seegene, Inc. | Processes using dual specificity oligonucleotide and dual specificity oligonucleotide |
CA2608474C (en) * | 2005-05-17 | 2019-11-12 | University Of Connecticut | Compositions and methods for immunomodulation in an organism |
WO2007106690A2 (en) | 2006-03-15 | 2007-09-20 | Siemens Healthcare Diagnostics Inc. | Degenerate nucleobase analogs |
CN101501251A (zh) * | 2006-06-16 | 2009-08-05 | 塞昆纳姆股份有限公司 | 扩增、检测和定量样品中核酸的方法和组合物 |
FI121428B (fi) | 2008-01-17 | 2010-11-15 | Mobidiag Oy | Laajakirjoisia alukkeita, testipakkaus ja menetelmä bakteerilajien havaitsemiseksi ja tunnistamiseksi |
GB201100150D0 (en) | 2011-01-06 | 2011-02-23 | Epistem Ltd | Mutational analysis |
GB201116131D0 (en) | 2011-09-19 | 2011-11-02 | Epistem Ltd | Probe |
CA2898613A1 (en) * | 2013-01-23 | 2014-07-31 | Brandeis University | Reagents for improving pcr accuracy |
-
2013
- 2013-10-01 GB GBGB1317355.4A patent/GB201317355D0/en not_active Ceased
-
2014
- 2014-09-30 EP EP14784097.9A patent/EP3052648B1/en active Active
- 2014-09-30 WO PCT/GB2014/052939 patent/WO2015049496A1/en active Application Filing
- 2014-09-30 JP JP2016519852A patent/JP6461114B2/ja not_active Expired - Fee Related
- 2014-09-30 CN CN201480065325.6A patent/CN105916999B/zh active Active
- 2014-09-30 MX MX2016004140A patent/MX2016004140A/es unknown
- 2014-09-30 BR BR112016007059A patent/BR112016007059A2/pt not_active IP Right Cessation
- 2014-09-30 KR KR1020167011542A patent/KR20160057486A/ko not_active Application Discontinuation
- 2014-09-30 US US15/026,296 patent/US10280452B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
WO2015049496A1 (en) | 2015-04-09 |
KR20160057486A (ko) | 2016-05-23 |
MX2016004140A (es) | 2016-06-06 |
US10280452B2 (en) | 2019-05-07 |
EP3052648A1 (en) | 2016-08-10 |
GB201317355D0 (en) | 2013-11-13 |
US20160237481A1 (en) | 2016-08-18 |
EP3052648B1 (en) | 2018-06-27 |
JP6461114B2 (ja) | 2019-01-30 |
JP2016533171A (ja) | 2016-10-27 |
CN105916999B (zh) | 2020-02-21 |
CN105916999A (zh) | 2016-08-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Mueller et al. | Widespread changes in nucleosome accessibility without changes in nucleosome occupancy during a rapid transcriptional induction | |
CY1117574T1 (el) | Αναλυση γονιδιωματικων κλασματων με χρηση των αριθμων πολυμορφισμων | |
WO2014116729A3 (en) | Haplotying of hla loci with ultra-deep shotgun sequencing | |
Talens et al. | Epigenetic variation during the adult lifespan: cross‐sectional and longitudinal data on monozygotic twin pairs | |
Worm Ørntoft et al. | Comparative analysis of 12 different kits for bisulfite conversion of circulating cell-free DNA | |
BR112016007059A2 (pt) | Análise de mutação que usa temperatura de fusão e bases universais | |
BR112016019940A2 (pt) | edição de genoma sem nucleases | |
BR112017008291A2 (pt) | oligonucleotídeos para edição de dna genômico | |
WO2015123430A3 (en) | Single molecule electronic multiplex snp assay and pcr analysis | |
BR112012030813A2 (pt) | "métodos e kits para detecção in situ de sequências de nucleotídeo" | |
BR112016019267B8 (pt) | Método para construir um perfil de dna, biblioteca de ácido nucléico, método para construir uma biblioteca de ácido nucléico, pluralidade de iniciadores e kit | |
BR112018001686A2 (pt) | conjunto de sonda para analisar uma amostra de dna e método para usar o mesmo | |
ZA201807405B (en) | Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor | |
EP4219739A3 (en) | Polymerase compositions, methods of making and using same | |
WO2015082449A3 (en) | Mutated dna polymerases with high selectivity and activity | |
GB201017978D0 (en) | Multiplex amplification and detection | |
PH12018502100A1 (en) | Surface-based detection of nucleic acid in a convection flow fluidic device | |
BR112017001500A2 (pt) | composições e métodos para a detecção e análise de tuberculose por micobactéria | |
BR112016015895A2 (pt) | Método de ensaio de gene mutante por meio da reação em cadeia da polimerase em tempo real usando inibição da atividade 5'-flap endonuclease | |
MX347790B (es) | Ensayos genómicos de brassica. | |
MX2017009833A (es) | Molecula de sustrato. | |
Shirak et al. | Sequence motifs capable of forming DNA stem–loop structures act as a replication diode | |
AR068526A1 (es) | Metodos para identificar variaciones de secuencias de nucleotidos (polimorfismos o mutaciones) en sujetos poliploides mediante un metodo de secuenciacion terminado con un dideoxinucleotido trifosfato (ddntp) | |
Naumova et al. | Developmental dynamics of the epigenome: A longitudinal study of three toddlers | |
Yu et al. | Reference materials for calibration of analytical biases in quantification of DNA methylation |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] | ||
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |