MX2021011847A - Genotecas de celulas unicas de alto rendimiento y metodos para elaborarlas y usarlas. - Google Patents

Genotecas de celulas unicas de alto rendimiento y metodos para elaborarlas y usarlas.

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Publication number
MX2021011847A
MX2021011847A MX2021011847A MX2021011847A MX2021011847A MX 2021011847 A MX2021011847 A MX 2021011847A MX 2021011847 A MX2021011847 A MX 2021011847A MX 2021011847 A MX2021011847 A MX 2021011847A MX 2021011847 A MX2021011847 A MX 2021011847A
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MX
Mexico
Prior art keywords
methods
making
nucleic acids
cell libraries
throughput single
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MX2021011847A
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English (en)
Inventor
Jay Shendure
Darren Cusanovich
Riza Daza
Frank Steemers
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Illumina Inc
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Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2021011847A publication Critical patent/MX2021011847A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

En la presente descripción se proporcionan métodos para preparar una genoteca de secuenciación que incluye ácidos nucleicos de una pluralidad de células únicas. En una modalidad, la genoteca de secuenciación incluye ácidos nucleicos que representan la accesibilidad de cromatina a partir de la pluralidad de células únicas. En una modalidad, los ácidos nucleicos incluyen tres secuencias de índice. En otra modalidad, la presente descripción proporciona métodos para caracterizar eventos raros en células y núcleos aislados.
MX2021011847A 2019-12-19 2020-12-18 Genotecas de celulas unicas de alto rendimiento y metodos para elaborarlas y usarlas. MX2021011847A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962950670P 2019-12-19 2019-12-19
PCT/US2020/066013 WO2021127436A2 (en) 2019-12-19 2020-12-18 High-throughput single-cell libraries and methods of making and of using

Publications (1)

Publication Number Publication Date
MX2021011847A true MX2021011847A (es) 2021-11-17

Family

ID=74191887

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021011847A MX2021011847A (es) 2019-12-19 2020-12-18 Genotecas de celulas unicas de alto rendimiento y metodos para elaborarlas y usarlas.

Country Status (12)

Country Link
US (1) US20220356461A1 (es)
EP (1) EP3927824A2 (es)
JP (1) JP2023508792A (es)
KR (1) KR20220118295A (es)
CN (1) CN114008199A (es)
AU (1) AU2020407641A1 (es)
BR (1) BR112021019640A2 (es)
CA (1) CA3134746A1 (es)
IL (1) IL286643A (es)
MX (1) MX2021011847A (es)
SG (1) SG11202109486QA (es)
WO (1) WO2021127436A2 (es)

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CN114121158A (zh) * 2021-12-01 2022-03-01 湖南大学 一种基于深度网络自适应的scRNA-seq细胞类型识别方法
WO2023137292A1 (en) * 2022-01-12 2023-07-20 Jumpcode Genomics, Inc. Methods and compositions for transcriptome analysis
CN118460688A (zh) * 2023-02-09 2024-08-09 中国人民解放军军事科学院军事医学研究院 多聚甲醛固定细胞的单细胞转录组及多重靶向蛋白质同时检测方法的建立

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Publication number Publication date
CN114008199A (zh) 2022-02-01
US20220356461A1 (en) 2022-11-10
WO2021127436A2 (en) 2021-06-24
WO2021127436A3 (en) 2021-07-29
BR112021019640A2 (pt) 2022-06-21
EP3927824A2 (en) 2021-12-29
SG11202109486QA (en) 2021-09-29
JP2023508792A (ja) 2023-03-06
CA3134746A1 (en) 2021-06-24
IL286643A (en) 2021-12-01
AU2020407641A1 (en) 2021-09-23
KR20220118295A (ko) 2022-08-25

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