BR112021019640A2 - Bibliotecas de células únicas de alto rendimento e métodos de preparo e uso - Google Patents

Bibliotecas de células únicas de alto rendimento e métodos de preparo e uso

Info

Publication number
BR112021019640A2
BR112021019640A2 BR112021019640A BR112021019640A BR112021019640A2 BR 112021019640 A2 BR112021019640 A2 BR 112021019640A2 BR 112021019640 A BR112021019640 A BR 112021019640A BR 112021019640 A BR112021019640 A BR 112021019640A BR 112021019640 A2 BR112021019640 A2 BR 112021019640A2
Authority
BR
Brazil
Prior art keywords
methods
preparation
single cell
nucleic acids
cell libraries
Prior art date
Application number
BR112021019640A
Other languages
English (en)
Inventor
Darren Cusanovich
Frank Steemers
Jay Shendure
Riza Daza
Original Assignee
Illumina Inc
Univ Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc, Univ Washington filed Critical Illumina Inc
Publication of BR112021019640A2 publication Critical patent/BR112021019640A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

bibliotecas de células únicas de alto rendimento e métodos de preparo e uso. a presente invenção se refere a métodos para preparar uma biblioteca de sequenciamento que inclui ácidos nucleicos de uma pluralidade de células únicas. em uma modalidade, a biblioteca de sequenciamento inclui ácidos nucleicos que representam a acessibilidade à cromatina a partir da pluralidade de células únicas. em uma modalidade, os ácidos nucleicos incluem três sequências de índice. em outra modalidade, a presente revelação fornece métodos para caracterizar eventos raros em células e núcleos isolados.
BR112021019640A 2019-12-19 2020-12-18 Bibliotecas de células únicas de alto rendimento e métodos de preparo e uso BR112021019640A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962950670P 2019-12-19 2019-12-19
PCT/US2020/066013 WO2021127436A2 (en) 2019-12-19 2020-12-18 High-throughput single-cell libraries and methods of making and of using

Publications (1)

Publication Number Publication Date
BR112021019640A2 true BR112021019640A2 (pt) 2022-06-21

Family

ID=74191887

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021019640A BR112021019640A2 (pt) 2019-12-19 2020-12-18 Bibliotecas de células únicas de alto rendimento e métodos de preparo e uso

Country Status (12)

Country Link
US (1) US20220356461A1 (pt)
EP (1) EP3927824A2 (pt)
JP (1) JP2023508792A (pt)
KR (1) KR20220118295A (pt)
CN (1) CN114008199A (pt)
AU (1) AU2020407641A1 (pt)
BR (1) BR112021019640A2 (pt)
CA (1) CA3134746A1 (pt)
IL (1) IL286643A (pt)
MX (1) MX2021011847A (pt)
SG (1) SG11202109486QA (pt)
WO (1) WO2021127436A2 (pt)

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WO2023137292A1 (en) * 2022-01-12 2023-07-20 Jumpcode Genomics, Inc. Methods and compositions for transcriptome analysis

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Also Published As

Publication number Publication date
CN114008199A (zh) 2022-02-01
SG11202109486QA (en) 2021-09-29
AU2020407641A1 (en) 2021-09-23
MX2021011847A (es) 2021-11-17
EP3927824A2 (en) 2021-12-29
WO2021127436A2 (en) 2021-06-24
WO2021127436A3 (en) 2021-07-29
JP2023508792A (ja) 2023-03-06
US20220356461A1 (en) 2022-11-10
KR20220118295A (ko) 2022-08-25
IL286643A (en) 2021-12-01
CA3134746A1 (en) 2021-06-24

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