MX2023001088A - Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso. - Google Patents
Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso.Info
- Publication number
- MX2023001088A MX2023001088A MX2023001088A MX2023001088A MX2023001088A MX 2023001088 A MX2023001088 A MX 2023001088A MX 2023001088 A MX2023001088 A MX 2023001088A MX 2023001088 A MX2023001088 A MX 2023001088A MX 2023001088 A MX2023001088 A MX 2023001088A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- making
- cell transcriptome
- throughput single
- nucleic acids
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 4
- 102000039446 nucleic acids Human genes 0.000 abstract 4
- 108020004707 nucleic acids Proteins 0.000 abstract 4
- 239000000203 mixture Substances 0.000 abstract 2
- 238000012163 sequencing technique Methods 0.000 abstract 2
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Se proporciona en la presente métodos para preparar una biblioteca de secuenciación que incluye ácidos nucleicos de una pluralidad de células individuales. En una modalidad, la biblioteca de secuenciación incluye ácidos nucleicos que representan los transcriptomas completos de la pluralidad de células individuales. En una modalidad, los ácidos nucleicos incluyen tres secuencias de índice. También se proporcionan en la presente composiciones, tal como composiciones que incluyen los ácidos nucleicos que tienen tres secuencias de índice.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201862680259P | 2018-06-04 | 2018-06-04 | |
US201962821678P | 2019-03-21 | 2019-03-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2023001088A true MX2023001088A (es) | 2023-03-16 |
Family
ID=68769215
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2019015262A MX2019015262A (es) | 2018-06-04 | 2019-06-04 | Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso. |
MX2023001088A MX2023001088A (es) | 2018-06-04 | 2019-12-16 | Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso. |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2019015262A MX2019015262A (es) | 2018-06-04 | 2019-06-04 | Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso. |
Country Status (15)
Country | Link |
---|---|
US (1) | US20210102194A1 (es) |
EP (2) | EP4269618A3 (es) |
JP (2) | JP6986103B2 (es) |
KR (2) | KR102393414B1 (es) |
CN (1) | CN111247248A (es) |
AU (2) | AU2019282158B2 (es) |
BR (1) | BR112019027750A2 (es) |
CA (1) | CA3072273A1 (es) |
DK (1) | DK3810774T3 (es) |
ES (1) | ES2966028T3 (es) |
FI (1) | FI3810774T3 (es) |
IL (1) | IL272234A (es) |
MX (2) | MX2019015262A (es) |
SG (1) | SG11202000905PA (es) |
WO (1) | WO2019236599A2 (es) |
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CN115461473A (zh) * | 2020-02-20 | 2022-12-09 | 加利福尼亚大学董事会 | 空间分辨单细胞rna测序方法 |
CN116249775A (zh) * | 2020-05-15 | 2023-06-09 | 合成Dna技术公司 | 连接偶联pcr的方法 |
CN111549025B (zh) * | 2020-06-08 | 2021-09-14 | 郑州大学第一附属医院 | 链置换引物和细胞转录组文库构建方法 |
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WO2022020728A1 (en) * | 2020-07-23 | 2022-01-27 | 10X Genomics, Inc. | Systems and methods for detecting and removing aggregates for calling cell-associated barcodes |
CN111996598A (zh) * | 2020-08-11 | 2020-11-27 | 杭州瀚因生命科技有限公司 | 一种单细胞染色质可及性的建库方法 |
CA3187250A1 (en) * | 2020-08-14 | 2022-02-17 | John Daniel WELLS | In situ library preparation for sequencing |
CN114606301A (zh) * | 2020-12-07 | 2022-06-10 | 中国科学院脑科学与智能技术卓越创新中心 | 一种单细胞转录组测序的方法 |
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EP4279609A1 (en) * | 2020-12-31 | 2023-11-22 | Beijing Institute of Genomics, Chinese Academy of Sciences (China National Center for Bioinformation) | Method and kit for labeling nucleic acid molecules |
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CN114958996B (zh) * | 2021-05-12 | 2022-12-20 | 浙江大学 | 一种超高通量单细胞测序试剂组合 |
CN113652475A (zh) * | 2021-07-19 | 2021-11-16 | 中国辐射防护研究院 | 一种重金属铀诱导仓鼠卵巢细胞损伤的研究方法 |
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2019
- 2019-06-04 JP JP2019570097A patent/JP6986103B2/ja active Active
- 2019-06-04 FI FIEP19814056.8T patent/FI3810774T3/fi active
- 2019-06-04 WO PCT/US2019/035422 patent/WO2019236599A2/en active Application Filing
- 2019-06-04 EP EP23187488.4A patent/EP4269618A3/en active Pending
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AU2021261918A1 (en) | 2021-12-02 |
KR20200035955A (ko) | 2020-04-06 |
WO2019236599A2 (en) | 2019-12-12 |
IL272234A (en) | 2020-03-31 |
EP3810774A2 (en) | 2021-04-28 |
ES2966028T3 (es) | 2024-04-18 |
RU2020102911A (ru) | 2021-07-27 |
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WO2019236599A3 (en) | 2020-02-20 |
JP2020533952A (ja) | 2020-11-26 |
BR112019027750A2 (pt) | 2022-06-14 |
AU2019282158B2 (en) | 2021-08-12 |
DK3810774T3 (da) | 2023-12-11 |
RU2020102911A3 (es) | 2021-08-27 |
JP2022031810A (ja) | 2022-02-22 |
FI3810774T3 (fi) | 2023-12-11 |
EP3810774A4 (en) | 2022-06-01 |
EP3810774B1 (en) | 2023-09-13 |
EP4269618A2 (en) | 2023-11-01 |
CA3072273A1 (en) | 2019-12-12 |
AU2019282158A1 (en) | 2020-01-16 |
US20210102194A1 (en) | 2021-04-08 |
JP6986103B2 (ja) | 2021-12-22 |
KR102507415B1 (ko) | 2023-03-07 |
SG11202000905PA (en) | 2020-02-27 |
MX2019015262A (es) | 2023-01-25 |
KR20220057665A (ko) | 2022-05-09 |
CN111247248A (zh) | 2020-06-05 |
KR102393414B1 (ko) | 2022-05-02 |
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