DK3810774T3 - Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse - Google Patents

Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse Download PDF

Info

Publication number
DK3810774T3
DK3810774T3 DK19814056.8T DK19814056T DK3810774T3 DK 3810774 T3 DK3810774 T3 DK 3810774T3 DK 19814056 T DK19814056 T DK 19814056T DK 3810774 T3 DK3810774 T3 DK 3810774T3
Authority
DK
Denmark
Prior art keywords
preparation
methods
single cell
cell transcriptome
put single
Prior art date
Application number
DK19814056.8T
Other languages
English (en)
Inventor
Frank J Steemers
Jay Shendure
Junyue Cao
Jacob Tome
Molly Gasperini
Original Assignee
Illumina Inc
Univ Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc, Univ Washington filed Critical Illumina Inc
Application granted granted Critical
Publication of DK3810774T3 publication Critical patent/DK3810774T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DK19814056.8T 2018-06-04 2019-06-04 Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse DK3810774T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862680259P 2018-06-04 2018-06-04
US201962821678P 2019-03-21 2019-03-21
PCT/US2019/035422 WO2019236599A2 (en) 2018-06-04 2019-06-04 High-throughput single-cell transcriptome libraries and methods of making and of using

Publications (1)

Publication Number Publication Date
DK3810774T3 true DK3810774T3 (da) 2023-12-11

Family

ID=68769215

Family Applications (1)

Application Number Title Priority Date Filing Date
DK19814056.8T DK3810774T3 (da) 2018-06-04 2019-06-04 Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse

Country Status (15)

Country Link
US (1) US20210102194A1 (da)
EP (2) EP4269618A3 (da)
JP (2) JP6986103B2 (da)
KR (2) KR102393414B1 (da)
CN (1) CN111247248A (da)
AU (2) AU2019282158B2 (da)
BR (1) BR112019027750A2 (da)
CA (1) CA3072273A1 (da)
DK (1) DK3810774T3 (da)
ES (1) ES2966028T3 (da)
FI (1) FI3810774T3 (da)
IL (1) IL272234A (da)
MX (2) MX2019015262A (da)
SG (1) SG11202000905PA (da)
WO (1) WO2019236599A2 (da)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
CN115461473A (zh) * 2020-02-20 2022-12-09 加利福尼亚大学董事会 空间分辨单细胞rna测序方法
CN116249775A (zh) * 2020-05-15 2023-06-09 合成Dna技术公司 连接偶联pcr的方法
CN111549025B (zh) * 2020-06-08 2021-09-14 郑州大学第一附属医院 链置换引物和细胞转录组文库构建方法
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2022020728A1 (en) * 2020-07-23 2022-01-27 10X Genomics, Inc. Systems and methods for detecting and removing aggregates for calling cell-associated barcodes
CN111996598A (zh) * 2020-08-11 2020-11-27 杭州瀚因生命科技有限公司 一种单细胞染色质可及性的建库方法
CA3187250A1 (en) * 2020-08-14 2022-02-17 John Daniel WELLS In situ library preparation for sequencing
CN114606301A (zh) * 2020-12-07 2022-06-10 中国科学院脑科学与智能技术卓越创新中心 一种单细胞转录组测序的方法
CN112592968B (zh) * 2020-12-27 2022-07-26 苏州科诺医学检验实验室有限公司 高通量测序用分子标签接头及其合成方法与应用
EP4279609A1 (en) * 2020-12-31 2023-11-22 Beijing Institute of Genomics, Chinese Academy of Sciences (China National Center for Bioinformation) Method and kit for labeling nucleic acid molecules
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
CN113160886B (zh) * 2021-04-02 2023-04-07 山东大学 基于单细胞Hi-C数据的细胞类型预测系统
CN114958996B (zh) * 2021-05-12 2022-12-20 浙江大学 一种超高通量单细胞测序试剂组合
CN113652475A (zh) * 2021-07-19 2021-11-16 中国辐射防护研究院 一种重金属铀诱导仓鼠卵巢细胞损伤的研究方法
WO2023044475A1 (en) * 2021-09-20 2023-03-23 Faeth Therapeutics, Inc. Methods of determining metabolic targets and designing nutrient modulating treatments
CN114107459B (zh) * 2021-11-11 2022-12-06 浙江大学 一种基于寡核苷酸链杂交标记的高通量单细胞测序方法
CA3227385A1 (en) * 2021-12-23 2023-06-29 Kazuo TORI Methods and compositions for producing cell-source identifiable collections of nucleic acids
WO2023137292A1 (en) * 2022-01-12 2023-07-20 Jumpcode Genomics, Inc. Methods and compositions for transcriptome analysis
WO2023159151A2 (en) * 2022-02-16 2023-08-24 Factorial Diagnostics, Inc. In situ library preparation
CA3223722A1 (en) 2022-04-07 2023-10-12 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2023225618A2 (en) * 2022-05-18 2023-11-23 Yale University Method for estimating a dynamic molecular program of a cell
CN114774527A (zh) * 2022-05-20 2022-07-22 良渚实验室 一种高通量单细胞转录组测序方法及其应用
EP4332234A1 (en) * 2022-08-29 2024-03-06 Ecole Polytechnique Federale De Lausanne (Epfl) Improved microfluidic transcriptome based screening methods
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
CN116497105B (zh) * 2023-06-28 2023-09-29 浙江大学 基于末端转移酶的单细胞转录组测序试剂盒及测序方法

Family Cites Families (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1989009835A1 (en) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
DE68927373T2 (de) 1988-06-24 1997-03-20 Amgen Inc Verfahren und mittel zum nachweis von nukleinsäuresequenzen
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
ATE138106T1 (de) 1988-07-20 1996-06-15 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5248607A (en) * 1988-10-27 1993-09-28 Ciba-Geigy Corporation Monoclonal antibodies and hybridomas specific for green-oat phytochrome
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
CA2035010C (en) 1990-01-26 1996-12-10 Keith C. Backman Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
JPH06197762A (ja) * 1992-12-28 1994-07-19 Iseki & Co Ltd タマネギのｄｎａ抽出法
EP0754240B1 (en) 1994-02-07 2003-08-20 Beckman Coulter, Inc. Ligase/polymerase-mediated genetic bit analysis of single nucleotide polymorphisms and its use in genetic analysis
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
CA2185239C (en) 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
WO1997007404A1 (en) * 1995-08-21 1997-02-27 THE GOVERMENT OF THE UNITED STATES OF AMERICA reprsented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Methods of assessing mhc class i expression and proteins capable of modulating class i expression
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
AU6846698A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid amplification
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
EP1368460B1 (en) 2000-07-07 2007-10-31 Visigen Biotechnologies, Inc. Real-time sequence determination
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20030082584A1 (en) * 2001-06-29 2003-05-01 Liang Shi Enzymatic ligation-based identification of transcript expression
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
SI3002289T1 (en) 2002-08-23 2018-07-31 Illumina Cambridge Limited MODIFIED NUCLEOTES FOR POLYUCULOTIDE SEQUENCING
CA2498764C (en) 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US9045796B2 (en) 2003-06-20 2015-06-02 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
JP2007525571A (ja) 2004-01-07 2007-09-06 ソレクサ リミテッド 修飾分子アレイ
EP3415641B1 (en) 2004-09-17 2023-11-01 Pacific Biosciences Of California, Inc. Method for analysis of molecules
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
EP1888743B1 (en) 2005-05-10 2011-08-03 Illumina Cambridge Limited Improved polymerases
WO2006138284A2 (en) 2005-06-15 2006-12-28 Callida Genomics, Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
WO2007133831A2 (en) 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
EP2021503A1 (en) 2006-03-17 2009-02-11 Solexa Ltd. Isothermal methods for creating clonal single molecule arrays
SG170802A1 (en) 2006-03-31 2011-05-30 Solexa Inc Systems and devices for sequence by synthesis analysis
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
ES2923759T3 (es) 2006-12-14 2022-09-30 Life Technologies Corp Aparato para medir analitos utilizando matrices de FET
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
DK3623481T3 (da) 2011-09-23 2021-11-15 Illumina Inc Sammensætninger til nukleinsyresekventering
US8778849B2 (en) 2011-10-28 2014-07-15 Illumina, Inc. Microarray fabrication system and method
US8938309B2 (en) 2012-01-16 2015-01-20 Greatbatch Ltd. Elevated hermetic feedthrough insulator adapted for side attachment of electrical conductors on the body fluid side of an active implantable medical device
US9193996B2 (en) 2012-04-03 2015-11-24 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
EP3919624A3 (en) 2013-07-01 2021-12-29 Illumina, Inc. Catalyst-free surface functionalization and polymer grafting
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
CN104032377A (zh) * 2014-06-30 2014-09-10 北京诺禾致源生物信息科技有限公司 单细胞转录组测序文库的构建方法及其应用
CN114805710B (zh) 2014-10-31 2023-11-17 伊鲁米纳剑桥有限公司 聚合物以及dna共聚物涂层
US10900065B2 (en) * 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
CN112126675B (zh) * 2015-01-12 2022-09-09 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
AU2016219328B2 (en) 2015-02-10 2022-04-21 Illumina, Inc. Methods and compositions for analyzing cellular components
WO2016154040A2 (en) * 2015-03-23 2016-09-29 The Regents Of The University Of California Biosynthetic labeling and separation of rna
WO2016172373A1 (en) * 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
WO2017164936A1 (en) * 2016-03-21 2017-09-28 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics in single cells
DK3488002T3 (da) * 2016-07-22 2021-06-21 Univ Oregon Health & Science Enkeltcelle-helgenombiblioteker og kombinatoriske indekseringsfremgangsmåder til fremstilling deraf
JP7234114B2 (ja) * 2016-12-29 2023-03-07 イルミナ インコーポレイテッド 細胞区画内の生体分子への直交アクセスおよび細胞区画内の生体分子のタグ付けのための分析システム

Also Published As

Publication number Publication date
AU2021261918A1 (en) 2021-12-02
KR20200035955A (ko) 2020-04-06
WO2019236599A2 (en) 2019-12-12
IL272234A (en) 2020-03-31
MX2023001088A (es) 2023-03-16
EP3810774A2 (en) 2021-04-28
ES2966028T3 (es) 2024-04-18
RU2020102911A (ru) 2021-07-27
EP4269618A3 (en) 2024-01-10
WO2019236599A3 (en) 2020-02-20
JP2020533952A (ja) 2020-11-26
BR112019027750A2 (pt) 2022-06-14
AU2019282158B2 (en) 2021-08-12
RU2020102911A3 (da) 2021-08-27
JP2022031810A (ja) 2022-02-22
FI3810774T3 (fi) 2023-12-11
EP3810774A4 (en) 2022-06-01
EP3810774B1 (en) 2023-09-13
EP4269618A2 (en) 2023-11-01
CA3072273A1 (en) 2019-12-12
AU2019282158A1 (en) 2020-01-16
US20210102194A1 (en) 2021-04-08
JP6986103B2 (ja) 2021-12-22
KR102507415B1 (ko) 2023-03-07
SG11202000905PA (en) 2020-02-27
MX2019015262A (es) 2023-01-25
KR20220057665A (ko) 2022-05-09
CN111247248A (zh) 2020-06-05
KR102393414B1 (ko) 2022-05-02

Similar Documents

Publication Publication Date Title
DK3810774T3 (da) Enkeltcelletransskriptom-biblioteker med højt throughput og fremgangsmåder til fremstilling og anvendelse
DK3568475T3 (da) Nukleinsyrekonserveringsopløsning og fremgangsmåder til anvendelse
DK3618863T3 (da) Anti-tigit-antistoffer og fremgangsmåder til anvendelse deraf
DK3788044T3 (da) RIP1-hæmmende forbindelser og fremgangsmåder til fremstilling og anvendelse heraf
DK3601358T3 (da) Anti-trem2-antistoffer og fremgangsmåder til anvendelse deraf
DK3761980T3 (da) Aminosyreforbindelser og fremgangsmåder til anvendelse
DK3384046T3 (da) Digitalt mikrofluidisk system til enkeltcelleisolering og karakterisering af analytter
DK3559009T3 (da) Sammensætninger og metoder til inhibition af arginaseaktivitet
DK3596216T3 (da) Systemer og fremgangsmåder til massiv parallel kombinatorisk analyse af enkeltceller
DK3488002T3 (da) Enkeltcelle-helgenombiblioteker og kombinatoriske indekseringsfremgangsmåder til fremstilling deraf
DK3285926T3 (da) Kit og fremgangsmåde til mikrobiologiske applikationer med høj kapacitet
DK3551660T3 (da) Anti-ctla-4-antistoffer og fremgangsmåder til anvendelse deraf
DK3247393T3 (da) Matrixmetalloproteasespaltelige og serinproteasespaltelige substrater og fremgangsmåder til anvendelse deraf
DK3529257T3 (da) Oxysteroler og fremgangsmåder til anvendelse deraf
DK3447137T3 (da) Flydende enzympræparat og fremgangsmåde til fremstilling deraf
DK3788045T3 (da) RIP1-hæmmende forbindelser og fremgangsmåder til fremstilling og anvendelse deraf
DK3259597T3 (da) Pvrig-polypeptider og fremgangsmåder til behandling
DK3397968T3 (da) Inhibitorer af urea-baseret prostata-specifikt membranantigen (PSMA) til billeddannelse og terapi
DK3317284T3 (da) Benzoxazepin-oxazolidinonforbindelser og fremgangsmåder til anvendelse
DK3325669T3 (da) Sammensætninger og fremgangsmåder til RNA-analyse
DK3250610T3 (da) Fcrn-antistoffer og fremgangsmåder til anvendelse heraf
DK3618928T3 (da) Anti-Sortilin-antistoffer og fremgangsmåder til anvendelse deraf
DK3394065T3 (da) Tetrahydropyranyl-amino-pyrrolopyrimidinon og fremgangsmåder til anvendelse deraf
DK3305310T3 (da) Onkolytisk virusformulering og fremgangsmåde til fremstilling deraf
HK1243513A1 (zh) 多威脅分析器陣列系統和使用方法