WO2019236599A3 - High-throughput single-cell transcriptome libraries and methods of making and of using - Google Patents

High-throughput single-cell transcriptome libraries and methods of making and of using Download PDF

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Publication number
WO2019236599A3
WO2019236599A3 PCT/US2019/035422 US2019035422W WO2019236599A3 WO 2019236599 A3 WO2019236599 A3 WO 2019236599A3 US 2019035422 W US2019035422 W US 2019035422W WO 2019236599 A3 WO2019236599 A3 WO 2019236599A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
making
cell transcriptome
throughput single
nucleic acids
Prior art date
Application number
PCT/US2019/035422
Other languages
French (fr)
Other versions
WO2019236599A2 (en
Inventor
Frank J. Steemers
Jay Shendure
Junyue CAO
Original Assignee
Illumina, Inc.
University Of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US16/625,100 priority Critical patent/US20210102194A1/en
Priority to ES19814056T priority patent/ES2966028T3/en
Priority to DK19814056.8T priority patent/DK3810774T3/en
Priority to EP23187488.4A priority patent/EP4269618A3/en
Priority to EP19814056.8A priority patent/EP3810774B1/en
Priority to RU2020102911A priority patent/RU2773318C2/en
Priority to MX2019015262A priority patent/MX2019015262A/en
Priority to BR112019027750A priority patent/BR112019027750A2/en
Priority to KR1020227014338A priority patent/KR102507415B1/en
Priority to KR1020207003217A priority patent/KR102393414B1/en
Application filed by Illumina, Inc., University Of Washington filed Critical Illumina, Inc.
Priority to CA3072273A priority patent/CA3072273A1/en
Priority to CN201980003786.3A priority patent/CN111247248A/en
Priority to SG11202000905PA priority patent/SG11202000905PA/en
Priority to FIEP19814056.8T priority patent/FI3810774T3/en
Priority to AU2019282158A priority patent/AU2019282158B2/en
Priority to JP2019570097A priority patent/JP6986103B2/en
Publication of WO2019236599A2 publication Critical patent/WO2019236599A2/en
Priority to IL272234A priority patent/IL272234A/en
Publication of WO2019236599A3 publication Critical patent/WO2019236599A3/en
Priority to AU2021261918A priority patent/AU2021261918A1/en
Priority to JP2021192377A priority patent/JP2022031810A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Abstract

Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the sequencing library includes nucleic acids that represent the whole transcriptomes from the plurality of single cells. In one embodiment, the nucleic acids include three index sequences. Also provided herein are compositions, such as compositions that include the nucleic acids having three index sequences.
PCT/US2019/035422 2018-06-04 2019-06-04 High-throughput single-cell transcriptome libraries and methods of making and of using WO2019236599A2 (en)

Priority Applications (19)

Application Number Priority Date Filing Date Title
CA3072273A CA3072273A1 (en) 2018-06-04 2019-06-04 High-throughput single-cell transcriptome libraries and methods of making and of using
ES19814056T ES2966028T3 (en) 2018-06-04 2019-06-04 High-throughput single-cell transcriptome libraries and methods of preparation and use
EP23187488.4A EP4269618A3 (en) 2018-06-04 2019-06-04 Methods of making high-throughput single-cell transcriptome libraries
EP19814056.8A EP3810774B1 (en) 2018-06-04 2019-06-04 Methods of making high-throughput single-cell transcriptome libraries
RU2020102911A RU2773318C2 (en) 2018-06-04 2019-06-04 Large-scale monocellular transcriptome libraries and methods for their production and use
MX2019015262A MX2019015262A (en) 2018-06-04 2019-06-04 High-throughput single-cell transcriptome libraries and methods of making and of using.
BR112019027750A BR112019027750A2 (en) 2018-06-04 2019-06-04 High-throughput single-cell transcriptonic libraries, and methods of fabrication and uses
CN201980003786.3A CN111247248A (en) 2018-06-04 2019-06-04 High throughput single cell transcriptome libraries and methods of making and using
KR1020207003217A KR102393414B1 (en) 2018-06-04 2019-06-04 High-throughput, high-volume single-cell transcriptome libraries and methods of making and using same
US16/625,100 US20210102194A1 (en) 2018-06-04 2019-06-04 High-throughput single-cell transcriptome libraries and methods of making and of using
DK19814056.8T DK3810774T3 (en) 2018-06-04 2019-06-04 HIGH-THROUGH-PUT SINGLE CELL TRANSCRIPTOME LIBRARIES AND METHODS OF PREPARATION AND USE
KR1020227014338A KR102507415B1 (en) 2018-06-04 2019-06-04 High-throughput single-cell transcriptome libraries and methods of making and of using
SG11202000905PA SG11202000905PA (en) 2018-06-04 2019-06-04 High-throughput single-cell transcriptome libraries and methods of making and of using
FIEP19814056.8T FI3810774T3 (en) 2018-06-04 2019-06-04 Methods of making high-throughput single-cell transcriptome libraries
AU2019282158A AU2019282158B2 (en) 2018-06-04 2019-06-04 High-throughput single-cell transcriptome libraries and methods of making and of using
JP2019570097A JP6986103B2 (en) 2018-06-04 2019-06-04 High-throughput single-cell transcriptome library and how to make and use it
IL272234A IL272234A (en) 2018-06-04 2020-01-24 High-throughput single-cell transcriptome libraries and methods of making and of using
AU2021261918A AU2021261918A1 (en) 2018-06-04 2021-11-04 High-Throughput Single-Cell Transcriptome Libraries and Methods of Making and of Using
JP2021192377A JP2022031810A (en) 2018-06-04 2021-11-26 High-throughput single-cell transcriptome libraries and methods of making and using the same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201862680259P 2018-06-04 2018-06-04
US62/680,259 2018-06-04
US201962821678P 2019-03-21 2019-03-21
US62/821,678 2019-03-21

Publications (2)

Publication Number Publication Date
WO2019236599A2 WO2019236599A2 (en) 2019-12-12
WO2019236599A3 true WO2019236599A3 (en) 2020-02-20

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PCT/US2019/035422 WO2019236599A2 (en) 2018-06-04 2019-06-04 High-throughput single-cell transcriptome libraries and methods of making and of using

Country Status (15)

Country Link
US (1) US20210102194A1 (en)
EP (2) EP4269618A3 (en)
JP (2) JP6986103B2 (en)
KR (2) KR102507415B1 (en)
CN (1) CN111247248A (en)
AU (2) AU2019282158B2 (en)
BR (1) BR112019027750A2 (en)
CA (1) CA3072273A1 (en)
DK (1) DK3810774T3 (en)
ES (1) ES2966028T3 (en)
FI (1) FI3810774T3 (en)
IL (1) IL272234A (en)
MX (2) MX2019015262A (en)
SG (1) SG11202000905PA (en)
WO (1) WO2019236599A2 (en)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
KR20220156837A (en) * 2020-02-20 2022-11-28 더 리전트 오브 더 유니버시티 오브 캘리포니아 Spatially resolved single cell RNA sequencing method
EP4150106A2 (en) * 2020-05-15 2023-03-22 Integrated Dna Technologies, Inc. Methods for ligation-coupled-pcr
CN111549025B (en) * 2020-06-08 2021-09-14 郑州大学第一附属医院 Strand displacement primer and cell transcriptome library construction method
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
EP4186060A4 (en) * 2020-07-23 2024-01-03 10X Genomics Inc Systems and methods for detecting and removing aggregates for calling cell-associated barcodes
CN111996598A (en) * 2020-08-11 2020-11-27 杭州瀚因生命科技有限公司 Method for establishing library of accessibility of single-cell chromatin
US20230265499A1 (en) * 2020-08-14 2023-08-24 Factorial Diagnostics, Inc. In Situ Library Preparation for Sequencing
CN112592968B (en) * 2020-12-27 2022-07-26 苏州科诺医学检验实验室有限公司 Molecular tag joint for high-throughput sequencing and synthesis method and application thereof
WO2022143221A1 (en) * 2020-12-31 2022-07-07 中国科学院北京基因组研究所(国家生物信息中心) Method and kit for labeling nucleic acid molecules
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
CN113160886B (en) * 2021-04-02 2023-04-07 山东大学 Cell type prediction system based on single cell Hi-C data
CN113106150B (en) * 2021-05-12 2022-05-24 浙江大学 Ultrahigh-throughput single cell sequencing method
CN113652475A (en) * 2021-07-19 2021-11-16 中国辐射防护研究院 Research method for inducing damage of hamster ovary cells by heavy metal uranium
WO2023044475A1 (en) * 2021-09-20 2023-03-23 Faeth Therapeutics, Inc. Methods of determining metabolic targets and designing nutrient modulating treatments
CN114107459B (en) * 2021-11-11 2022-12-06 浙江大学 High-throughput single cell sequencing method based on oligonucleotide chain hybridization marker
CA3227385A1 (en) * 2021-12-23 2023-06-29 Kazuo TORI Methods and compositions for producing cell-source identifiable collections of nucleic acids
WO2023137292A1 (en) * 2022-01-12 2023-07-20 Jumpcode Genomics, Inc. Methods and compositions for transcriptome analysis
WO2023159151A2 (en) * 2022-02-16 2023-08-24 Factorial Diagnostics, Inc. In situ library preparation
WO2023196572A1 (en) 2022-04-07 2023-10-12 Illumina Singapore Pte. Ltd. Altered cytidine deaminases and methods of use
WO2023225618A2 (en) * 2022-05-18 2023-11-23 Yale University Method for estimating a dynamic molecular program of a cell
CN114774527A (en) * 2022-05-20 2022-07-22 良渚实验室 High-throughput single-cell transcriptome sequencing method and application thereof
EP4332234A1 (en) * 2022-08-29 2024-03-06 Ecole Polytechnique Federale De Lausanne (Epfl) Improved microfluidic transcriptome based screening methods
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
CN116497105B (en) * 2023-06-28 2023-09-29 浙江大学 Single-cell transcriptome sequencing kit based on terminal transferase and sequencing method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015200609A1 (en) * 2014-06-26 2015-12-30 Illumina, Inc. Library preparation of tagged nucleic acid using single tube add-on protocol
WO2016130704A2 (en) * 2015-02-10 2016-08-18 Illumina, Inc. Methods and compositions for analyzing cellular components
US20180023119A1 (en) * 2016-07-22 2018-01-25 Illumina, Inc. Single cell whole genome libraries and combinatorial indexing methods of making thereof
WO2018125982A1 (en) * 2016-12-29 2018-07-05 Illumina, Inc. Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments

Family Cites Families (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1989009835A1 (en) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
DE68927373T2 (en) 1988-06-24 1997-03-20 Amgen Inc METHOD AND MEANS FOR DETECTING NUCLEIC ACID SEQUENCES
JP2955759B2 (en) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド Methods for amplifying and detecting nucleic acid sequences
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5248607A (en) * 1988-10-27 1993-09-28 Ciba-Geigy Corporation Monoclonal antibodies and hybridomas specific for green-oat phytochrome
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
ATE137269T1 (en) 1990-01-26 1996-05-15 Abbott Lab IMPROVED METHOD FOR AMPLIFICATION OF NUCLIC ACID TARGET SEQUENCE USABLE FOR POLYMERASE AND LIGASED CHAIN REACTION
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
JPH06197762A (en) * 1992-12-28 1994-07-19 Iseki & Co Ltd Method for extracting dna of onion
CA2182517C (en) 1994-02-07 2001-08-21 Theo Nikiforov Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
KR100230718B1 (en) 1994-03-16 1999-11-15 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 Isothermal strand displacement nucleic acid amplification
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
CA2229938A1 (en) * 1995-08-21 1997-02-27 Koichi Suzuki Methods of assessing mhc class i expression and proteins capable of modulating class i expression
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
ES2563643T3 (en) 1997-04-01 2016-03-15 Illumina Cambridge Limited Nucleic acid sequencing method
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
AR021833A1 (en) 1998-09-30 2002-08-07 Applied Research Systems METHODS OF AMPLIFICATION AND SEQUENCING OF NUCLEIC ACID
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
EP1368460B1 (en) 2000-07-07 2007-10-31 Visigen Biotechnologies, Inc. Real-time sequence determination
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (en) 2000-12-08 2003-09-24 Applied Research Systems ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS IN A SOLID SUPPORT
US20030082584A1 (en) * 2001-06-29 2003-05-01 Liang Shi Enzymatic ligation-based identification of transcript expression
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
WO2004027025A2 (en) 2002-09-20 2004-04-01 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
EP1636337A4 (en) 2003-06-20 2007-07-04 Illumina Inc Methods and compositions for whole genome amplification and genotyping
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
US7476503B2 (en) 2004-09-17 2009-01-13 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis
EP1828412B2 (en) 2004-12-13 2019-01-09 Illumina Cambridge Limited Improved method of nucleotide detection
JP4990886B2 (en) 2005-05-10 2012-08-01 ソレックサ リミテッド Improved polymerase
US20090264299A1 (en) 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
CA2611671C (en) 2005-06-15 2013-10-08 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
JP5180845B2 (en) 2006-02-24 2013-04-10 カリダ・ジェノミックス・インコーポレイテッド High-throughput genomic sequencing on DNA arrays
US20080009420A1 (en) 2006-03-17 2008-01-10 Schroth Gary P Isothermal methods for creating clonal single molecule arrays
CN101460953B (en) 2006-03-31 2012-05-30 索雷克萨公司 Systems and devices for sequence by synthesis analysis
WO2008051530A2 (en) 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
WO2012170936A2 (en) 2011-06-09 2012-12-13 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
HUE056246T2 (en) 2011-09-23 2022-02-28 Illumina Inc Compositions for nucleic acid sequencing
CA3003082C (en) 2011-10-28 2020-12-15 Illumina, Inc. Microarray fabrication system and method
US8653384B2 (en) 2012-01-16 2014-02-18 Greatbatch Ltd. Co-fired hermetically sealed feedthrough with alumina substrate and platinum filled via for an active implantable medical device
WO2013151622A1 (en) 2012-04-03 2013-10-10 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
EP3919624A3 (en) 2013-07-01 2021-12-29 Illumina, Inc. Catalyst-free surface functionalization and polymer grafting
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
CN104032377A (en) * 2014-06-30 2014-09-10 北京诺禾致源生物信息科技有限公司 Construction method of single cell transcriptome sequencing library and application of construction method
CN114805710B (en) 2014-10-31 2023-11-17 伊鲁米纳剑桥有限公司 Polymer and DNA copolymer coating
US10900065B2 (en) * 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
SG11201705615UA (en) * 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
WO2016154040A2 (en) * 2015-03-23 2016-09-29 The Regents Of The University Of California Biosynthetic labeling and separation of rna
CN107580632B (en) * 2015-04-23 2021-12-28 贝克顿迪金森公司 Methods and compositions for whole transcriptome amplification
WO2017164936A1 (en) * 2016-03-21 2017-09-28 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics in single cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015200609A1 (en) * 2014-06-26 2015-12-30 Illumina, Inc. Library preparation of tagged nucleic acid using single tube add-on protocol
WO2016130704A2 (en) * 2015-02-10 2016-08-18 Illumina, Inc. Methods and compositions for analyzing cellular components
US20180023119A1 (en) * 2016-07-22 2018-01-25 Illumina, Inc. Single cell whole genome libraries and combinatorial indexing methods of making thereof
WO2018125982A1 (en) * 2016-12-29 2018-07-05 Illumina, Inc. Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments

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