SG11202000905PA - High-throughput single-cell transcriptome libraries and methods of making and of using - Google Patents
High-throughput single-cell transcriptome libraries and methods of making and of usingInfo
- Publication number
- SG11202000905PA SG11202000905PA SG11202000905PA SG11202000905PA SG11202000905PA SG 11202000905P A SG11202000905P A SG 11202000905PA SG 11202000905P A SG11202000905P A SG 11202000905PA SG 11202000905P A SG11202000905P A SG 11202000905PA SG 11202000905P A SG11202000905P A SG 11202000905PA
- Authority
- SG
- Singapore
- Prior art keywords
- making
- methods
- cell transcriptome
- throughput single
- transcriptome libraries
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862680259P | 2018-06-04 | 2018-06-04 | |
| US201962821678P | 2019-03-21 | 2019-03-21 | |
| PCT/US2019/035422 WO2019236599A2 (en) | 2018-06-04 | 2019-06-04 | High-throughput single-cell transcriptome libraries and methods of making and of using |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202000905PA true SG11202000905PA (en) | 2020-02-27 |
Family
ID=68769215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202000905PA SG11202000905PA (en) | 2018-06-04 | 2019-06-04 | High-throughput single-cell transcriptome libraries and methods of making and of using |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US20210102194A1 (es) |
| EP (2) | EP4269618A3 (es) |
| JP (2) | JP6986103B2 (es) |
| KR (2) | KR102393414B1 (es) |
| CN (1) | CN111247248A (es) |
| AU (2) | AU2019282158B2 (es) |
| BR (1) | BR112019027750A2 (es) |
| CA (1) | CA3072273A1 (es) |
| DK (1) | DK3810774T3 (es) |
| ES (1) | ES2966028T3 (es) |
| FI (1) | FI3810774T3 (es) |
| IL (1) | IL272234A (es) |
| MX (2) | MX2019015262A (es) |
| SG (1) | SG11202000905PA (es) |
| WO (1) | WO2019236599A2 (es) |
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| US10900065B2 (en) | 2014-11-14 | 2021-01-26 | University Of Washington | Methods and kits for labeling cellular molecules |
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| US20230212656A1 (en) * | 2020-02-20 | 2023-07-06 | The Regents Of The University Of California | Methods of spatially resolved single cell sequencing |
| EP4150106A4 (en) * | 2020-05-15 | 2024-07-10 | Integrated Dna Tech Inc | PROCEDURE FOR LIGATION-COUPLED PCR |
| CN111549025B (zh) * | 2020-06-08 | 2021-09-14 | 郑州大学第一附属医院 | 链置换引物和细胞转录组文库构建方法 |
| WO2021252617A1 (en) | 2020-06-09 | 2021-12-16 | Illumina, Inc. | Methods for increasing yield of sequencing libraries |
| JP2023536699A (ja) * | 2020-07-22 | 2023-08-29 | アルゲン バイオテクノロジーズ，インク． | 薬物の有効性を判定するための方法およびシステム |
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| CN111996598A (zh) * | 2020-08-11 | 2020-11-27 | 杭州瀚因生命科技有限公司 | 一种单细胞染色质可及性的建库方法 |
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| CN114606301A (zh) * | 2020-12-07 | 2022-06-10 | 中国科学院脑科学与智能技术卓越创新中心 | 一种单细胞转录组测序的方法 |
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| EP4279609A1 (en) * | 2020-12-31 | 2023-11-22 | Beijing Institute of Genomics, Chinese Academy of Sciences (China National Center for Bioinformation) | Method and kit for labeling nucleic acid molecules |
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| CN113160886B (zh) * | 2021-04-02 | 2023-04-07 | 山东大学 | 基于单细胞Hi-C数据的细胞类型预测系统 |
| CN114958996B (zh) * | 2021-05-12 | 2022-12-20 | 浙江大学 | 一种超高通量单细胞测序试剂组合 |
| CN113652475A (zh) * | 2021-07-19 | 2021-11-16 | 中国辐射防护研究院 | 一种重金属铀诱导仓鼠卵巢细胞损伤的研究方法 |
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2019
- 2019-06-04 KR KR1020207003217A patent/KR102393414B1/ko active IP Right Grant
- 2019-06-04 WO PCT/US2019/035422 patent/WO2019236599A2/en active Application Filing
- 2019-06-04 KR KR1020227014338A patent/KR102507415B1/ko active IP Right Grant
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| KR102393414B1 (ko) | 2022-05-02 |
| AU2019282158B2 (en) | 2021-08-12 |
| RU2020102911A3 (es) | 2021-08-27 |
| EP3810774A4 (en) | 2022-06-01 |
| WO2019236599A2 (en) | 2019-12-12 |
| US20210102194A1 (en) | 2021-04-08 |
| DK3810774T3 (da) | 2023-12-11 |
| EP4269618A3 (en) | 2024-01-10 |
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| MX2023001088A (es) | 2023-03-16 |
| CN111247248A (zh) | 2020-06-05 |
| EP3810774A2 (en) | 2021-04-28 |
| EP4269618A2 (en) | 2023-11-01 |
| IL272234A (en) | 2020-03-31 |
| JP2022031810A (ja) | 2022-02-22 |
| AU2019282158A1 (en) | 2020-01-16 |
| WO2019236599A3 (en) | 2020-02-20 |
| AU2021261918A1 (en) | 2021-12-02 |
| CA3072273A1 (en) | 2019-12-12 |
| RU2020102911A (ru) | 2021-07-27 |
| ES2966028T3 (es) | 2024-04-18 |
| EP3810774B1 (en) | 2023-09-13 |
| KR20200035955A (ko) | 2020-04-06 |
| MX2019015262A (es) | 2023-01-25 |
| FI3810774T3 (fi) | 2023-12-11 |
| KR20220057665A (ko) | 2022-05-09 |
| KR102507415B1 (ko) | 2023-03-07 |
| JP6986103B2 (ja) | 2021-12-22 |
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