MX2021011847A - High-throughput single-cell libraries and methods of making and of using. - Google Patents
High-throughput single-cell libraries and methods of making and of using.Info
- Publication number
- MX2021011847A MX2021011847A MX2021011847A MX2021011847A MX2021011847A MX 2021011847 A MX2021011847 A MX 2021011847A MX 2021011847 A MX2021011847 A MX 2021011847A MX 2021011847 A MX2021011847 A MX 2021011847A MX 2021011847 A MX2021011847 A MX 2021011847A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- making
- nucleic acids
- cell libraries
- throughput single
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the sequencing library includes nucleic acids that represent the chromatin accessibility from the plurality of single cells. In one embodiment, the nucleic acids include three index sequences. In another embodiment, the present disclosure provides methods for characterizing rare events in isolated cells and nuclei.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962950670P | 2019-12-19 | 2019-12-19 | |
PCT/US2020/066013 WO2021127436A2 (en) | 2019-12-19 | 2020-12-18 | High-throughput single-cell libraries and methods of making and of using |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2021011847A true MX2021011847A (en) | 2021-11-17 |
Family
ID=74191887
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2021011847A MX2021011847A (en) | 2019-12-19 | 2020-12-18 | High-throughput single-cell libraries and methods of making and of using. |
Country Status (12)
Country | Link |
---|---|
US (1) | US20220356461A1 (en) |
EP (1) | EP3927824A2 (en) |
JP (1) | JP2023508792A (en) |
KR (1) | KR20220118295A (en) |
CN (1) | CN114008199A (en) |
AU (1) | AU2020407641A1 (en) |
BR (1) | BR112021019640A2 (en) |
CA (1) | CA3134746A1 (en) |
IL (1) | IL286643A (en) |
MX (1) | MX2021011847A (en) |
SG (1) | SG11202109486QA (en) |
WO (1) | WO2021127436A2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20240024835A (en) * | 2021-06-24 | 2024-02-26 | 일루미나, 인코포레이티드 | Methods and compositions for bead-based combinatorial indexing of nucleic acids |
WO2023137292A1 (en) * | 2022-01-12 | 2023-07-20 | Jumpcode Genomics, Inc. | Methods and compositions for transcriptome analysis |
Family Cites Families (81)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
CA1341584C (en) | 1988-04-06 | 2008-11-18 | Bruce Wallace | Method of amplifying and detecting nucleic acid sequences |
WO1989009835A1 (en) | 1988-04-08 | 1989-10-19 | The Salk Institute For Biological Studies | Ligase-based amplification method |
JP2801051B2 (en) | 1988-06-24 | 1998-09-21 | アムジエン・インコーポレーテツド | Methods and reagents for detecting nucleobase sequences |
US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
WO1990001069A1 (en) | 1988-07-20 | 1990-02-08 | Segev Diagnostics, Inc. | Process for amplifying and detecting nucleic acid sequences |
US5185243A (en) | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
CA2044616A1 (en) | 1989-10-26 | 1991-04-27 | Roger Y. Tsien | Dna sequencing |
AU635105B2 (en) | 1990-01-26 | 1993-03-11 | Abbott Laboratories | Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
US5573907A (en) | 1990-01-26 | 1996-11-12 | Abbott Laboratories | Detecting and amplifying target nucleic acids using exonucleolytic activity |
US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
JP3175110B2 (en) | 1994-02-07 | 2001-06-11 | オーキッド・バイオサイエンシーズ・インコーポレイテッド | Genetic bit analysis of ligase / polymerase mediated single nucleotide polymorphisms and their use in genetic analysis |
US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
AU687535B2 (en) | 1994-03-16 | 1998-02-26 | Gen-Probe Incorporated | Isothermal strand displacement nucleic acid amplification |
US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
ES2563643T3 (en) | 1997-04-01 | 2016-03-15 | Illumina Cambridge Limited | Nucleic acid sequencing method |
US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
AR021833A1 (en) | 1998-09-30 | 2002-08-07 | Applied Research Systems | METHODS OF AMPLIFICATION AND SEQUENCING OF NUCLEIC ACID |
US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
US7611869B2 (en) | 2000-02-07 | 2009-11-03 | Illumina, Inc. | Multiplexed methylation detection methods |
US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
CA2415897A1 (en) | 2000-07-07 | 2002-01-17 | Susan H. Hardin | Real-time sequence determination |
EP1354064A2 (en) | 2000-12-01 | 2003-10-22 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
AR031640A1 (en) | 2000-12-08 | 2003-09-24 | Applied Research Systems | ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS IN A SOLID SUPPORT |
US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
DK3363809T3 (en) | 2002-08-23 | 2020-05-04 | Illumina Cambridge Ltd | MODIFIED NUCLEOTIDES FOR POLYNUCLEOTIDE SEQUENCE |
CA2498764C (en) | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
WO2005003304A2 (en) | 2003-06-20 | 2005-01-13 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
EP1701785A1 (en) | 2004-01-07 | 2006-09-20 | Solexa Ltd. | Modified molecular arrays |
CN101914620B (en) | 2004-09-17 | 2014-02-12 | 加利福尼亚太平洋生命科学公司 | Method for analysis of molecules |
EP1828412B2 (en) | 2004-12-13 | 2019-01-09 | Illumina Cambridge Limited | Improved method of nucleotide detection |
JP4990886B2 (en) | 2005-05-10 | 2012-08-01 | ソレックサ リミテッド | Improved polymerase |
EP1907571B1 (en) | 2005-06-15 | 2017-04-26 | Complete Genomics Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
EP2495337A1 (en) | 2006-02-24 | 2012-09-05 | Callida Genomics, Inc. | High throughput genome sequencing on DNA arrays |
SG10201405158QA (en) | 2006-02-24 | 2014-10-30 | Callida Genomics Inc | High throughput genome sequencing on dna arrays |
EP2021503A1 (en) | 2006-03-17 | 2009-02-11 | Solexa Ltd. | Isothermal methods for creating clonal single molecule arrays |
US8241573B2 (en) | 2006-03-31 | 2012-08-14 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
WO2008051530A2 (en) | 2006-10-23 | 2008-05-02 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
WO2008070352A2 (en) | 2006-10-27 | 2008-06-12 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
ES2923759T3 (en) | 2006-12-14 | 2022-09-30 | Life Technologies Corp | Apparatus for measuring analytes using FET arrays |
EP2121983A2 (en) | 2007-02-02 | 2009-11-25 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
WO2010003132A1 (en) | 2008-07-02 | 2010-01-07 | Illumina Cambridge Ltd. | Using populations of beads for the fabrication of arrays on surfaces |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
WO2012061832A1 (en) | 2010-11-05 | 2012-05-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
WO2012170936A2 (en) | 2011-06-09 | 2012-12-13 | Illumina, Inc. | Patterned flow-cells useful for nucleic acid analysis |
WO2013044018A1 (en) | 2011-09-23 | 2013-03-28 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
CA3003082C (en) | 2011-10-28 | 2020-12-15 | Illumina, Inc. | Microarray fabrication system and method |
US8938309B2 (en) | 2012-01-16 | 2015-01-20 | Greatbatch Ltd. | Elevated hermetic feedthrough insulator adapted for side attachment of electrical conductors on the body fluid side of an active implantable medical device |
EP4219012A1 (en) | 2012-04-03 | 2023-08-02 | Illumina, Inc. | Method of imaging a substrate comprising fluorescent features and use of the method in nucleic acid sequencing |
US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
CN105339503B (en) | 2013-05-23 | 2020-04-10 | 斯坦福大学托管董事会 | Transposition to native chromatin for personal epigenomics |
DK3017065T3 (en) | 2013-07-01 | 2018-11-26 | Illumina Inc | Catalyst-free Surface functionalization and polymer grafting |
US9677132B2 (en) | 2014-01-16 | 2017-06-13 | Illumina, Inc. | Polynucleotide modification on solid support |
US10017759B2 (en) * | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
JP6808617B2 (en) | 2014-10-17 | 2021-01-06 | イルミナ ケンブリッジ リミテッド | Dislocations that maintain continuity |
PL3212684T3 (en) | 2014-10-31 | 2020-10-19 | Illumina Cambridge Limited | Polymers and dna copolymer coatings |
EP3725893A1 (en) | 2015-02-10 | 2020-10-21 | Illumina, Inc. | Compositions for analyzing cellular components |
US11535883B2 (en) | 2016-07-22 | 2022-12-27 | Illumina, Inc. | Single cell whole genome libraries and combinatorial indexing methods of making thereof |
CA3067435C (en) * | 2018-05-17 | 2023-09-12 | Illumina, Inc. | High-throughput single-cell sequencing with reduced amplification bias |
KR20210134598A (en) | 2019-03-01 | 2021-11-10 | 일루미나, 인코포레이티드 | High-throughput single-nuclear and single-cell libraries and methods of making and using same |
-
2020
- 2020-12-18 CA CA3134746A patent/CA3134746A1/en active Pending
- 2020-12-18 CN CN202080026206.5A patent/CN114008199A/en active Pending
- 2020-12-18 WO PCT/US2020/066013 patent/WO2021127436A2/en unknown
- 2020-12-18 US US17/441,741 patent/US20220356461A1/en active Pending
- 2020-12-18 BR BR112021019640A patent/BR112021019640A2/en unknown
- 2020-12-18 KR KR1020217030969A patent/KR20220118295A/en unknown
- 2020-12-18 SG SG11202109486QA patent/SG11202109486QA/en unknown
- 2020-12-18 EP EP20842799.7A patent/EP3927824A2/en active Pending
- 2020-12-18 MX MX2021011847A patent/MX2021011847A/en unknown
- 2020-12-18 JP JP2021557409A patent/JP2023508792A/en active Pending
- 2020-12-18 AU AU2020407641A patent/AU2020407641A1/en active Pending
-
2021
- 2021-09-23 IL IL286643A patent/IL286643A/en unknown
Also Published As
Publication number | Publication date |
---|---|
AU2020407641A1 (en) | 2021-09-23 |
CA3134746A1 (en) | 2021-06-24 |
JP2023508792A (en) | 2023-03-06 |
EP3927824A2 (en) | 2021-12-29 |
IL286643A (en) | 2021-12-01 |
WO2021127436A3 (en) | 2021-07-29 |
SG11202109486QA (en) | 2021-09-29 |
KR20220118295A (en) | 2022-08-25 |
WO2021127436A2 (en) | 2021-06-24 |
CN114008199A (en) | 2022-02-01 |
BR112021019640A2 (en) | 2022-06-21 |
US20220356461A1 (en) | 2022-11-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
MX2023010271A (en) | High-throughput single-cell sequencing with reduced amplification bias. | |
Heidari et al. | Genome-wide map of regulatory interactions in the human genome | |
Muerdter et al. | STARR-seq—principles and applications | |
Lazar et al. | Epigenetic maintenance of topological domains in the highly rearranged gibbon genome | |
Phillips-Cremins et al. | Architectural protein subclasses shape 3D organization of genomes during lineage commitment | |
DeMare et al. | The genomic landscape of cohesin-associated chromatin interactions | |
NZ759895A (en) | Single cell whole genome libraries for methylation sequencing | |
MX2021011847A (en) | High-throughput single-cell libraries and methods of making and of using. | |
Van Bortle et al. | Nuclear organization and genome function | |
Denissov et al. | Mll2 is required for H3K4 trimethylation on bivalent promoters in embryonic stem cells, whereas Mll1 is redundant | |
Liu | Linking telomere regulation to stem cell pluripotency | |
EP4289996A3 (en) | Nucleic acid indexing techniques | |
MX2017010142A (en) | Systems and methods for determining structural variation and phasing using variant call data. | |
Zheng et al. | Identification of lamin B–regulated chromatin regions based on chromatin landscapes | |
MX2019003765A (en) | Phenotype/disease specific gene ranking using curated, gene library and network based data structures. | |
WO2019033062A3 (en) | Tagging nucleic acid molecules from single cells for phased sequencing | |
Kapoor et al. | Regional centromeres in the yeast Candida lusitaniae lack pericentromeric heterochromatin | |
ATE524563T1 (en) | SYSTEM FOR PRODUCING SYNTHETIC PROMOTORS | |
Zylicz et al. | G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst | |
Bleckwehl et al. | Enhancer-associated H3K4 methylation safeguards in vitro germline competence | |
EP4282979A3 (en) | Modular polypeptide libraries and methods of making and using same | |
NZ601353A (en) | Cell lines and methods for making and using them | |
MX2023008931A (en) | Complex surface-bound transposome complexes. | |
Panigrahi et al. | Higher-order orchestration of hematopoiesis: is cohesin a new player? | |
Liu et al. | Arrayed mutant haploid embryonic stem cell libraries facilitate phenotype-driven genetic screens |