MX2023008931A - Complejos de transposoma ligados a superficie de complejo. - Google Patents

Complejos de transposoma ligados a superficie de complejo.

Info

Publication number
MX2023008931A
MX2023008931A MX2023008931A MX2023008931A MX2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A MX 2023008931 A MX2023008931 A MX 2023008931A
Authority
MX
Mexico
Prior art keywords
transposome complexes
complex surface
compositions
bound transposome
methods
Prior art date
Application number
MX2023008931A
Other languages
English (en)
Inventor
Andrew Slatter
Esther Musgrave-Brown
Susan C Verity
Niall Gormley
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of MX2023008931A publication Critical patent/MX2023008931A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente descripción se relaciona con métodos, composiciones, y kits para generar una genoteca de fragmentos de ácido nucleico marcados sin usar la amplificación por PCR, que incluye métodos y composiciones para la fragmentación y marcación de ácidos nucleicos (p. ej., ADN) usando complejos de transposoma inmovilizados en soporte sólido.
MX2023008931A 2019-01-11 2021-03-26 Complejos de transposoma ligados a superficie de complejo. MX2023008931A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962791509P 2019-01-11 2019-01-11
US201962840610P 2019-04-30 2019-04-30

Publications (1)

Publication Number Publication Date
MX2023008931A true MX2023008931A (es) 2023-08-10

Family

ID=69172787

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2021003625A MX2021003625A (es) 2019-01-11 2020-01-10 Complejos de transposoma ligados a superficie de complejo.
MX2023008931A MX2023008931A (es) 2019-01-11 2021-03-26 Complejos de transposoma ligados a superficie de complejo.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2021003625A MX2021003625A (es) 2019-01-11 2020-01-10 Complejos de transposoma ligados a superficie de complejo.

Country Status (12)

Country Link
US (3) US11685946B2 (es)
EP (1) EP3908672A1 (es)
JP (1) JP2022516821A (es)
KR (1) KR20210114918A (es)
CN (1) CN112930405A (es)
AU (1) AU2020206443A1 (es)
BR (1) BR112021006038A2 (es)
CA (1) CA3114732A1 (es)
IL (1) IL281664B1 (es)
MX (2) MX2021003625A (es)
SG (1) SG11202102918WA (es)
WO (1) WO2020144373A1 (es)

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EP4192951A1 (en) * 2020-08-06 2023-06-14 Illumina, Inc. Preparation of rna and dna sequencing libraries using bead-linked transposomes
CN112795563A (zh) * 2021-03-23 2021-05-14 上海欣百诺生物科技有限公司 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法
AU2022252197A1 (en) * 2021-03-29 2023-09-21 Illumina, Inc. Improved methods of library preparation
AU2022249289A1 (en) 2021-03-31 2023-08-17 Illumina Cambridge Limited Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction
KR20220141246A (ko) * 2021-04-09 2022-10-19 주식회사 셀레믹스 간소화된 차세대 염기서열 분석 라이브러리 전처리 방법
WO2023225095A1 (en) * 2022-05-18 2023-11-23 Illumina Cambridge Limited Preparation of size-controlled nucleic acid fragments
WO2024024583A1 (ja) * 2022-07-25 2024-02-01 東洋紡株式会社 次世代シーケンサー解析等のための断片化及びタグメント化方法
CN116515977B (zh) * 2023-06-28 2024-04-16 浙江大学 基于单端接头转座酶的单细胞基因组测序试剂盒和方法

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CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
CN100462433C (zh) 2000-07-07 2009-02-18 维西根生物技术公司 实时序列测定
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
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EP2789383B1 (en) 2004-01-07 2023-05-03 Illumina Cambridge Limited Molecular arrays
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GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
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AU2012212148B8 (en) * 2011-02-02 2017-07-06 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
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EP3845640A3 (en) 2014-04-15 2021-09-01 Illumina, Inc. Modified tranposases for improved insertion sequence bias and increased dna input tolerence
WO2016037361A1 (zh) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 试剂盒及其在核酸测序中的用途
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ES2855748T3 (es) * 2016-07-12 2021-09-24 Hoffmann La Roche Enriquecimiento de diana de extensión de cebador
FI3452621T3 (fi) 2017-02-21 2022-12-15 Tagmentaatio käyttäen immobilisoituja transposomeja linkkereillä
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Also Published As

Publication number Publication date
CN112930405A (zh) 2021-06-08
CA3114732A1 (en) 2020-07-16
WO2020144373A1 (en) 2020-07-16
AU2020206443A1 (en) 2021-04-15
KR20210114918A (ko) 2021-09-24
US11685946B2 (en) 2023-06-27
MX2021003625A (es) 2021-05-27
EP3908672A1 (en) 2021-11-17
JP2022516821A (ja) 2022-03-03
IL281664B1 (en) 2024-03-01
SG11202102918WA (en) 2021-04-29
IL281664A (en) 2021-05-31
US11306348B2 (en) 2022-04-19
US20210269856A1 (en) 2021-09-02
US20200224246A1 (en) 2020-07-16
BR112021006038A2 (pt) 2021-10-26
US20230340568A1 (en) 2023-10-26

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