SG11202102918WA - Complex surface-bound transposome complexes - Google Patents

Complex surface-bound transposome complexes

Info

Publication number
SG11202102918WA
SG11202102918WA SG11202102918WA SG11202102918WA SG11202102918WA SG 11202102918W A SG11202102918W A SG 11202102918WA SG 11202102918W A SG11202102918W A SG 11202102918WA SG 11202102918W A SG11202102918W A SG 11202102918WA SG 11202102918W A SG11202102918W A SG 11202102918WA
Authority
SG
Singapore
Prior art keywords
complex surface
transposome complexes
bound transposome
bound
complexes
Prior art date
Application number
SG11202102918WA
Inventor
Andrew Slatter
Brown Esther Musgrave-
Susan C Verity
Niall Gormley
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of SG11202102918WA publication Critical patent/SG11202102918WA/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/507Recombinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/191Modifications characterised by incorporating an adaptor
SG11202102918WA 2019-01-11 2020-01-10 Complex surface-bound transposome complexes SG11202102918WA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962791509P 2019-01-11 2019-01-11
US201962840610P 2019-04-30 2019-04-30
PCT/EP2020/050612 WO2020144373A1 (en) 2019-01-11 2020-01-10 Complex surface-bound transposome complexes

Publications (1)

Publication Number Publication Date
SG11202102918WA true SG11202102918WA (en) 2021-04-29

Family

ID=69172787

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202102918WA SG11202102918WA (en) 2019-01-11 2020-01-10 Complex surface-bound transposome complexes

Country Status (11)

Country Link
US (3) US11685946B2 (en)
EP (1) EP3908672A1 (en)
JP (1) JP2022516821A (en)
KR (1) KR20210114918A (en)
CN (1) CN112930405A (en)
AU (1) AU2020206443A1 (en)
CA (1) CA3114732A1 (en)
IL (1) IL281664B1 (en)
MX (1) MX2021003625A (en)
SG (1) SG11202102918WA (en)
WO (1) WO2020144373A1 (en)

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IL300112A (en) * 2020-08-06 2023-03-01 Illumina Inc Preparation of rna and dna sequencing libraries using bead-linked transposomes
CN112795563A (en) * 2021-03-23 2021-05-14 上海欣百诺生物科技有限公司 Use and method of biotinylated transposomes for recovering CUT & Tag or ATAC-seq products
WO2022212269A1 (en) * 2021-03-29 2022-10-06 Illumina, Inc. Improved methods of library preparation
WO2022212402A1 (en) 2021-03-31 2022-10-06 Illumina, Inc. Methods of preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction
WO2022216133A1 (en) * 2021-04-09 2022-10-13 주식회사 셀레믹스 Simplified next-generation sequencing library preparation method
WO2023225095A1 (en) * 2022-05-18 2023-11-23 Illumina Cambridge Limited Preparation of size-controlled nucleic acid fragments
WO2024024583A1 (en) * 2022-07-25 2024-02-01 東洋紡株式会社 Fragmentation and tagmentation method for next-generation sequence analysis and like

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US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
EP3795577A1 (en) 2002-08-23 2021-03-24 Illumina Cambridge Limited Modified nucleotides
EP2789383B1 (en) 2004-01-07 2023-05-03 Illumina Cambridge Limited Molecular arrays
GB2423819B (en) 2004-09-17 2008-02-06 Pacific Biosciences California Apparatus and method for analysis of molecules
GB0427236D0 (en) 2004-12-13 2005-01-12 Solexa Ltd Improved method of nucleotide detection
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
WO2007123744A2 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
CN103443338B (en) * 2011-02-02 2017-09-22 华盛顿大学商业化中心 Massively parallel continguity mapping
US9644199B2 (en) 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
RU2698125C2 (en) 2013-08-19 2019-08-22 Эбботт Молекьюлар Инк. Libraries for next generation sequencing
AU2015247779B2 (en) 2014-04-15 2021-06-24 Illumina, Inc. Modified transposases for improved insertion sequence bias and increased DNA input tolerance
WO2016037361A1 (en) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 Kit and use thereof in nucleic acid sequencing
KR102472027B1 (en) 2014-10-17 2022-11-30 일루미나 케임브리지 리미티드 Contiguity preserving transposition
US11453875B2 (en) 2015-05-28 2022-09-27 Illumina Cambridge Limited Surface-based tagmentation
US20170283864A1 (en) * 2016-03-31 2017-10-05 Agilent Technologies, Inc. Use of transposase and y adapters to fragment and tag dna
US10907204B2 (en) * 2016-07-12 2021-02-02 Roche Sequencing Solutions, Inc. Primer extension target enrichment
NZ748776A (en) * 2017-02-21 2022-08-26 Illumina Cambridge Ltd Tagmentation using immobilized transposomes with linkers
CA3062174A1 (en) * 2017-05-08 2018-11-15 Illumina, Inc. Universal short adapters for indexing of polynucleotide samples
US10844372B2 (en) * 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP4180534A1 (en) * 2017-11-02 2023-05-17 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
SG11202003885UA (en) * 2017-11-08 2020-05-28 Twinstrand Biosciences Inc Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters

Also Published As

Publication number Publication date
CN112930405A (en) 2021-06-08
US11685946B2 (en) 2023-06-27
US20230340568A1 (en) 2023-10-26
IL281664B1 (en) 2024-03-01
CA3114732A1 (en) 2020-07-16
US20210269856A1 (en) 2021-09-02
IL281664A (en) 2021-05-31
EP3908672A1 (en) 2021-11-17
US11306348B2 (en) 2022-04-19
WO2020144373A1 (en) 2020-07-16
MX2021003625A (en) 2021-05-27
US20200224246A1 (en) 2020-07-16
AU2020206443A1 (en) 2021-04-15
KR20210114918A (en) 2021-09-24
JP2022516821A (en) 2022-03-03

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