MX2018015450A - Identificacion de anticuerpos humanos con maduracion de afinidad. - Google Patents
Identificacion de anticuerpos humanos con maduracion de afinidad.Info
- Publication number
- MX2018015450A MX2018015450A MX2018015450A MX2018015450A MX2018015450A MX 2018015450 A MX2018015450 A MX 2018015450A MX 2018015450 A MX2018015450 A MX 2018015450A MX 2018015450 A MX2018015450 A MX 2018015450A MX 2018015450 A MX2018015450 A MX 2018015450A
- Authority
- MX
- Mexico
- Prior art keywords
- primers
- region
- nucleic acids
- amplified
- sequence
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/515—Complete light chain, i.e. VL + CL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Las regiones V de inmunoglobulina específica de antígeno se identifican a partir de una colección de ácidos nucleicos ampliada utilizando una reacción de cadena de polimerasa que usa cebadores directos específicos de secuencia líder. El uso de cebadores de secuencia líder permite que todas las secuencias de la región V se amplíen (que incluyen aquellas con mutaciones del terminal 5´ extensivo) sin la pérdida de la secuencia del segmento génico V 5´ original. Estas colecciones pueden monitorearse para regiones V específicas de antígeno que utilizan células eucariotas diseñadas para expresar los ácidos nucleicos codificadores de la región V ampliada o utilizando técnicas de expresión de fago bacteriano. En este último caso, se produce una segunda colección de región V utilizando un conjunto de cebadores de región V 5´ mayor que el convencional. Los errores de secuencia introducidos en los productos de ampliación mediante este método se corrigen utilizando información de secuencia obtenida en los productos amplificados por los cebadores de la región V para monitorear la colección creada utilizando los cebadores de secuencia líder. La información de secuencia de aminoácidos de fragmentos de inmunoglobulinas del donante puede utilizarse para ayudar a identificar ácidos nucleicos que codifican las cadenas pesada y liviana de anticuerpos del donante así como también para diseñar cebadores con el objeto de amplificar dichos ácidos nucleicos.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161506749P | 2011-07-12 | 2011-07-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2018015450A true MX2018015450A (es) | 2021-11-16 |
Family
ID=47506915
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014000431A MX361600B (es) | 2011-07-12 | 2012-07-12 | Identificación de anticuerpos humanos con maduración de afinidad. |
| MX2018015450A MX2018015450A (es) | 2011-07-12 | 2014-01-10 | Identificacion de anticuerpos humanos con maduracion de afinidad. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014000431A MX361600B (es) | 2011-07-12 | 2012-07-12 | Identificación de anticuerpos humanos con maduración de afinidad. |
Country Status (11)
| Country | Link |
|---|---|
| US (6) | US9234030B2 (es) |
| EP (2) | EP2731965A4 (es) |
| JP (1) | JP6731702B2 (es) |
| KR (1) | KR102139388B1 (es) |
| CN (2) | CN107090459A (es) |
| AU (2) | AU2012281078B2 (es) |
| CA (1) | CA2841475C (es) |
| IL (2) | IL230358B (es) |
| MX (2) | MX361600B (es) |
| RU (2) | RU2636045C2 (es) |
| WO (1) | WO2013009967A2 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2012281078B2 (en) * | 2011-07-12 | 2017-05-04 | Xbiotech Inc. | Identifying affinity-matured human antibodies |
| GB2525568B (en) | 2013-03-15 | 2020-10-14 | Abvitro Llc | Single cell barcoding for antibody discovery |
| GB201316644D0 (en) * | 2013-09-19 | 2013-11-06 | Kymab Ltd | Expression vector production & High-Throughput cell screening |
| EP3165605B1 (en) * | 2014-05-30 | 2019-11-06 | National University Corporation University Of Toyama | TCR cDNA AMPLIFICATION METHOD |
| AU2015271749B2 (en) | 2014-06-03 | 2018-03-01 | Xbiotech Inc. | Compositions and methods for treating and preventing Staphylococcus aureus infections |
| MX2017003382A (es) | 2014-09-15 | 2017-11-20 | Abvitro Llc | Secuenciacion de bibliotecas de nucleotidos de alto rendimiento. |
| US10975146B2 (en) | 2018-06-29 | 2021-04-13 | Cedars-Sinai Medical Center | Interleukin-1 inhibition for combination treatment of pancreatic cancer |
| US20200392578A1 (en) * | 2019-06-14 | 2020-12-17 | The Regents Of The University Of California | Methods of sequencing antibody chains from hybridomas and kits for practicing same |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001212019B2 (en) * | 2000-03-10 | 2006-12-14 | Large Scale Biology Corporation | Self antigen vaccines for treating b cell lymphomas and other cancers |
| AU2002953381A0 (en) * | 2002-12-18 | 2003-01-09 | Diatech Pty Ltd | In vivo affinity maturation scheme |
| CA2532556A1 (en) * | 2003-07-29 | 2005-02-24 | Genentech, Inc. | Assay for human anti cd20 antibodies and uses therefor |
| TWI333977B (en) * | 2003-09-18 | 2010-12-01 | Symphogen As | Method for linking sequences of interest |
| PE20071101A1 (es) | 2005-08-31 | 2007-12-21 | Amgen Inc | Polipeptidos y anticuerpos |
| JP5205597B2 (ja) * | 2007-06-01 | 2013-06-05 | 静岡県 | １細胞レベルでの抗体遺伝子の解析・同定方法 |
| AU2009298501A1 (en) * | 2008-09-30 | 2010-04-08 | Abbvie Inc. | Improved antibody libraries |
| CA2751669C (en) | 2009-02-20 | 2017-11-21 | F. Hoffmann-La Roche Ag | Method for obtaining immunoglobulin encoding nucleic acid |
| AU2012281078B2 (en) * | 2011-07-12 | 2017-05-04 | Xbiotech Inc. | Identifying affinity-matured human antibodies |
-
2012
- 2012-07-12 AU AU2012281078A patent/AU2012281078B2/en active Active
- 2012-07-12 CN CN201610954453.7A patent/CN107090459A/zh active Pending
- 2012-07-12 JP JP2014520314A patent/JP6731702B2/ja active Active
- 2012-07-12 EP EP12811637.3A patent/EP2731965A4/en not_active Withdrawn
- 2012-07-12 RU RU2014102617A patent/RU2636045C2/ru active
- 2012-07-12 US US13/547,497 patent/US9234030B2/en active Active
- 2012-07-12 EP EP15196994.6A patent/EP3034513B1/en active Active
- 2012-07-12 CA CA2841475A patent/CA2841475C/en active Active
- 2012-07-12 KR KR1020147003416A patent/KR102139388B1/ko active IP Right Grant
- 2012-07-12 RU RU2017134418A patent/RU2762685C2/ru active
- 2012-07-12 MX MX2014000431A patent/MX361600B/es active IP Right Grant
- 2012-07-12 CN CN201280034673.8A patent/CN103687873B/zh active Active
- 2012-07-12 WO PCT/US2012/046431 patent/WO2013009967A2/en active Application Filing
-
2014
- 2014-01-07 IL IL230358A patent/IL230358B/en active IP Right Grant
- 2014-01-10 MX MX2018015450A patent/MX2018015450A/es unknown
-
2015
- 2015-11-12 US US14/939,454 patent/US9453217B2/en active Active
- 2015-11-13 US US14/940,537 patent/US9394536B2/en active Active
-
2016
- 2016-08-26 US US15/248,413 patent/US9670483B2/en active Active
-
2017
- 2017-05-02 US US15/584,360 patent/US20170233457A1/en not_active Abandoned
- 2017-08-03 AU AU2017210585A patent/AU2017210585B2/en active Active
-
2018
- 2018-11-13 IL IL262977A patent/IL262977B/en active IP Right Grant
-
2019
- 2019-11-19 US US16/688,666 patent/US20200071388A1/en not_active Abandoned
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