IT1394539B1 - Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso - Google Patents

Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso

Info

Publication number
IT1394539B1
IT1394539B1 ITMI2009A000877A ITMI20090877A IT1394539B1 IT 1394539 B1 IT1394539 B1 IT 1394539B1 IT MI2009A000877 A ITMI2009A000877 A IT MI2009A000877A IT MI20090877 A ITMI20090877 A IT MI20090877A IT 1394539 B1 IT1394539 B1 IT 1394539B1
Authority
IT
Italy
Prior art keywords
liofilization
polymerase
stabilizer
ready
preparation
Prior art date
Application number
ITMI2009A000877A
Other languages
English (en)
Inventor
Luigi Roveda
Maurizio Gramegna
Luca Ugo De
Original Assignee
Sentinel Ch S P A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ITMI2009A000877A priority Critical patent/IT1394539B1/it
Application filed by Sentinel Ch S P A filed Critical Sentinel Ch S P A
Priority to CA2761059A priority patent/CA2761059C/en
Priority to BRPI1014980A priority patent/BRPI1014980A2/pt
Priority to PL10724727T priority patent/PL2432873T3/pl
Priority to JP2012511278A priority patent/JP2012527226A/ja
Priority to ES10724727.2T priority patent/ES2478890T3/es
Priority to US13/318,835 priority patent/US9273303B2/en
Priority to KR1020117026117A priority patent/KR20120042727A/ko
Priority to EP10724727.2A priority patent/EP2432873B1/en
Priority to AU2010251184A priority patent/AU2010251184B2/en
Priority to PCT/EP2010/056877 priority patent/WO2010133628A1/en
Priority to PT107247272T priority patent/PT2432873E/pt
Priority to CN2010800195495A priority patent/CN102414315A/zh
Priority to MX2011011616A priority patent/MX2011011616A/es
Publication of ITMI20090877A1 publication Critical patent/ITMI20090877A1/it
Application granted granted Critical
Publication of IT1394539B1 publication Critical patent/IT1394539B1/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
ITMI2009A000877A 2009-05-19 2009-05-19 Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso IT1394539B1 (it)

Priority Applications (14)

Application Number Priority Date Filing Date Title
ITMI2009A000877A IT1394539B1 (it) 2009-05-19 2009-05-19 Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso
EP10724727.2A EP2432873B1 (en) 2009-05-19 2010-05-19 A dried and stabilized ready-to-use composition containing nucleic acid polymerization enzymes for molecular biology applications
PL10724727T PL2432873T3 (pl) 2009-05-19 2010-05-19 Suszona i stabilizowana kompozycja gotowa do użycia zawierająca enzymy do polimeryzacji kwasu nukleinowego do zastosowań biologii molekularnej
JP2012511278A JP2012527226A (ja) 2009-05-19 2010-05-19 分子生物学的用途のための、核酸重合酵素を含有してなる、乾燥及び安定化したすぐ使用できる組成物
ES10724727.2T ES2478890T3 (es) 2009-05-19 2010-05-19 Composición lista para usar seca y estabilizada que contiene enzimas de polimerización de ácidos nucleicos para aplicaciones en biología molecular
US13/318,835 US9273303B2 (en) 2009-05-19 2010-05-19 Dried and stabilized ready-to-use composition containing nucleic acid polymerization enzymes for molecular biology applications
CA2761059A CA2761059C (en) 2009-05-19 2010-05-19 A dried and stabilized ready-to-use composition containing nucleic acid polymerization enzymes for molecular biology applications
BRPI1014980A BRPI1014980A2 (pt) 2009-05-19 2010-05-19 composição seca e estabilizada, pronta para uso, contendo enzimas de polimerização de ácido nucléico para aplicações de biologia molecular
AU2010251184A AU2010251184B2 (en) 2009-05-19 2010-05-19 A dried and stabilized ready-to-use composition containing nucleic acid polymerization enzymes for molecular biology applications
PCT/EP2010/056877 WO2010133628A1 (en) 2009-05-19 2010-05-19 A dried and stabilized ready-to-use composition containing nucleic acid polymerization enzymes for molecular biology applications
PT107247272T PT2432873E (pt) 2009-05-19 2010-05-19 Composição seca e estabilizada pronta a usar contendo enzimas de polimerização de ácido nucleico para aplicações em biologia molecular
CN2010800195495A CN102414315A (zh) 2009-05-19 2010-05-19 用于分子生物学应用的包含核酸聚合酶的干燥和稳定的即用组合物
MX2011011616A MX2011011616A (es) 2009-05-19 2010-05-19 Composicion seca y estabilizada lista para el uso que contiene enzimas de polimerizacion de acido nucleico para aplicaciones de biologia molecular.
KR1020117026117A KR20120042727A (ko) 2009-05-19 2010-05-19 분자생물학 적용을 위한 핵산 중합 효소를 포함하는 건조되고 안정화된 즉시사용가능한 조성물

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI2009A000877A IT1394539B1 (it) 2009-05-19 2009-05-19 Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso

Publications (2)

Publication Number Publication Date
ITMI20090877A1 ITMI20090877A1 (it) 2010-11-20
IT1394539B1 true IT1394539B1 (it) 2012-07-05

Family

ID=41527695

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI2009A000877A IT1394539B1 (it) 2009-05-19 2009-05-19 Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso

Country Status (14)

Country Link
US (1) US9273303B2 (it)
EP (1) EP2432873B1 (it)
JP (1) JP2012527226A (it)
KR (1) KR20120042727A (it)
CN (1) CN102414315A (it)
AU (1) AU2010251184B2 (it)
BR (1) BRPI1014980A2 (it)
CA (1) CA2761059C (it)
ES (1) ES2478890T3 (it)
IT (1) IT1394539B1 (it)
MX (1) MX2011011616A (it)
PL (1) PL2432873T3 (it)
PT (1) PT2432873E (it)
WO (1) WO2010133628A1 (it)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104620102A (zh) * 2012-09-13 2015-05-13 深江化成株式会社 保存方法以及保存容器
CN103849548A (zh) * 2012-12-03 2014-06-11 三星电子株式会社 用于扩增核酸的试剂容器及其制备方法、存储试剂的方法和用于核酸分析的微流体系统
CN103898077B (zh) * 2012-12-24 2017-01-11 财团法人工业技术研究院 单离的脱氧核糖核酸聚合酶、套组及其应用
EP3194629A4 (en) * 2014-09-17 2018-03-07 Theranos, Inc. Diagnostic methods and compositions
US9856524B2 (en) * 2014-12-04 2018-01-02 Shaofeng Ding Lyophilized integrated composition for storage and manipulation of pyrophosphorolysis activated polymerization
US9868944B2 (en) * 2014-12-19 2018-01-16 Roche Molecular Systems, Inc. Reaction mixtures
US10584371B2 (en) * 2015-02-13 2020-03-10 Seegene, Inc. Method for lyophilization of composition for multiple target nucleic acid sequence amplification reaction
JP2022060807A (ja) * 2020-10-05 2022-04-15 キヤノンメディカルシステムズ株式会社 乾燥試薬、試薬キット、乾燥試薬の製造方法、分析方法及び核酸増幅方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1230300A (en) * 1983-06-06 1987-12-15 Michael K. Hoskins Stabilized isoenzyme control product
US4806343A (en) 1986-03-13 1989-02-21 University Of Southwestern Louisiana Cryogenic protectant for proteins
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
GB8826429D0 (en) * 1988-11-11 1988-12-14 Univ Leeds Ind Service Ltd Enzyme stabilisation systems
US5593824A (en) * 1994-09-02 1997-01-14 Pharmacia Biotech, Inc. Biological reagent spheres
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
EP1423688B1 (en) * 2001-08-24 2019-04-03 Sensortec Limited Methods for producing highly sensitive potentiometric sensors
CN1335395A (zh) * 2001-09-04 2002-02-13 湖南尤特尔生化有限公司 高活性纤维素酶组合物及其制备方法
US7407747B2 (en) * 2002-10-15 2008-08-05 Applera Corporation Method for drying dye-terminator sequencing reagents
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
CN102943085A (zh) * 2005-03-15 2013-02-27 维莱尼姆公司 纤维素酶、编码它们的核酸及其制备和应用的方法
US20090269852A1 (en) * 2005-09-09 2009-10-29 Masako Shinjoh Novel gene gms 01

Also Published As

Publication number Publication date
BRPI1014980A2 (pt) 2015-10-27
PL2432873T3 (pl) 2014-10-31
AU2010251184A1 (en) 2011-11-24
EP2432873A1 (en) 2012-03-28
PT2432873E (pt) 2014-07-31
AU2010251184B2 (en) 2014-05-29
WO2010133628A1 (en) 2010-11-25
CA2761059A1 (en) 2010-11-25
ES2478890T3 (es) 2014-07-23
US9273303B2 (en) 2016-03-01
AU2010251184A2 (en) 2011-12-01
EP2432873B1 (en) 2014-04-23
CA2761059C (en) 2018-10-30
ITMI20090877A1 (it) 2010-11-20
KR20120042727A (ko) 2012-05-03
US20120064536A1 (en) 2012-03-15
JP2012527226A (ja) 2012-11-08
MX2011011616A (es) 2012-01-12
CN102414315A (zh) 2012-04-11

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