PT2432873E - Composição seca e estabilizada pronta a usar contendo enzimas de polimerização de ácido nucleico para aplicações em biologia molecular - Google Patents

Composição seca e estabilizada pronta a usar contendo enzimas de polimerização de ácido nucleico para aplicações em biologia molecular Download PDF

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Publication number
PT2432873E
PT2432873E PT107247272T PT10724727T PT2432873E PT 2432873 E PT2432873 E PT 2432873E PT 107247272 T PT107247272 T PT 107247272T PT 10724727 T PT10724727 T PT 10724727T PT 2432873 E PT2432873 E PT 2432873E
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PT
Portugal
Prior art keywords
dried
nucleic acid
composition containing
molecular biology
containing nucleic
Prior art date
Application number
PT107247272T
Other languages
English (en)
Inventor
Maurizio Gramegna
Ugo De Luca
Luigi Roveda
Original Assignee
Sentinel Ch S P A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sentinel Ch S P A filed Critical Sentinel Ch S P A
Publication of PT2432873E publication Critical patent/PT2432873E/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
PT107247272T 2009-05-19 2010-05-19 Composição seca e estabilizada pronta a usar contendo enzimas de polimerização de ácido nucleico para aplicações em biologia molecular PT2432873E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI2009A000877A IT1394539B1 (it) 2009-05-19 2009-05-19 Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso

Publications (1)

Publication Number Publication Date
PT2432873E true PT2432873E (pt) 2014-07-31

Family

ID=41527695

Family Applications (1)

Application Number Title Priority Date Filing Date
PT107247272T PT2432873E (pt) 2009-05-19 2010-05-19 Composição seca e estabilizada pronta a usar contendo enzimas de polimerização de ácido nucleico para aplicações em biologia molecular

Country Status (14)

Country Link
US (1) US9273303B2 (pt)
EP (1) EP2432873B1 (pt)
JP (1) JP2012527226A (pt)
KR (1) KR20120042727A (pt)
CN (1) CN102414315A (pt)
AU (1) AU2010251184B2 (pt)
BR (1) BRPI1014980A2 (pt)
CA (1) CA2761059C (pt)
ES (1) ES2478890T3 (pt)
IT (1) IT1394539B1 (pt)
MX (1) MX2011011616A (pt)
PL (1) PL2432873T3 (pt)
PT (1) PT2432873E (pt)
WO (1) WO2010133628A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6246127B2 (ja) * 2012-09-13 2017-12-13 深江化成株式会社 保存方法及び保存容器
CN103849548A (zh) * 2012-12-03 2014-06-11 三星电子株式会社 用于扩增核酸的试剂容器及其制备方法、存储试剂的方法和用于核酸分析的微流体系统
US9309501B2 (en) 2012-12-24 2016-04-12 Industrial Technology Research Institute Isolated DNA polymerases, kits and applications thereof
CN113736863A (zh) * 2014-09-17 2021-12-03 赛拉诺斯知识产权有限责任公司 诊断方法和组合物
US9856524B2 (en) * 2014-12-04 2018-01-02 Shaofeng Ding Lyophilized integrated composition for storage and manipulation of pyrophosphorolysis activated polymerization
US9868944B2 (en) * 2014-12-19 2018-01-16 Roche Molecular Systems, Inc. Reaction mixtures
WO2016129951A1 (en) * 2015-02-13 2016-08-18 Seegene, Inc. Method for lyophilization of composition for multilple target nucleic acid sequence amplification reaction
JP2022060807A (ja) * 2020-10-05 2022-04-15 キヤノンメディカルシステムズ株式会社 乾燥試薬、試薬キット、乾燥試薬の製造方法、分析方法及び核酸増幅方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1230300A (en) * 1983-06-06 1987-12-15 Michael K. Hoskins Stabilized isoenzyme control product
US4806343A (en) * 1986-03-13 1989-02-21 University Of Southwestern Louisiana Cryogenic protectant for proteins
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
GB8826429D0 (en) * 1988-11-11 1988-12-14 Univ Leeds Ind Service Ltd Enzyme stabilisation systems
US5593824A (en) * 1994-09-02 1997-01-14 Pharmacia Biotech, Inc. Biological reagent spheres
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
AU2002321531B2 (en) * 2001-08-24 2007-12-13 Sensortec Limited Methods for producing highly sensitive potentiometric sensors
CN1335395A (zh) * 2001-09-04 2002-02-13 湖南尤特尔生化有限公司 高活性纤维素酶组合物及其制备方法
US7407747B2 (en) * 2002-10-15 2008-08-05 Applera Corporation Method for drying dye-terminator sequencing reagents
US20060099567A1 (en) 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
NZ594810A (en) * 2005-03-15 2012-12-21 Verenium Corp Cellulases, nucleic acids encoding them and methods for making and using them
WO2007028611A2 (en) * 2005-09-09 2007-03-15 Dsm Ip Assets B.V. Process for biomass production using a microorganism with a disrupted glucose-dehydrogenase gene (gms 01)

Also Published As

Publication number Publication date
CA2761059C (en) 2018-10-30
US9273303B2 (en) 2016-03-01
AU2010251184A1 (en) 2011-11-24
CN102414315A (zh) 2012-04-11
JP2012527226A (ja) 2012-11-08
PL2432873T3 (pl) 2014-10-31
WO2010133628A1 (en) 2010-11-25
AU2010251184B2 (en) 2014-05-29
EP2432873B1 (en) 2014-04-23
BRPI1014980A2 (pt) 2015-10-27
CA2761059A1 (en) 2010-11-25
AU2010251184A2 (en) 2011-12-01
KR20120042727A (ko) 2012-05-03
US20120064536A1 (en) 2012-03-15
MX2011011616A (es) 2012-01-12
ITMI20090877A1 (it) 2010-11-20
EP2432873A1 (en) 2012-03-28
IT1394539B1 (it) 2012-07-05
ES2478890T3 (es) 2014-07-23

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