ATE233816T1 - Gereinigtes thermostabiles nukleinsäure- polymeraseenzym aus thermotoga maritima - Google Patents

Gereinigtes thermostabiles nukleinsäure- polymeraseenzym aus thermotoga maritima

Info

Publication number
ATE233816T1
ATE233816T1 AT91915802T AT91915802T ATE233816T1 AT E233816 T1 ATE233816 T1 AT E233816T1 AT 91915802 T AT91915802 T AT 91915802T AT 91915802 T AT91915802 T AT 91915802T AT E233816 T1 ATE233816 T1 AT E233816T1
Authority
AT
Austria
Prior art keywords
nucleic acid
thermotoga maritima
zyme
acid polymerase
purified thermostable
Prior art date
Application number
AT91915802T
Other languages
English (en)
Inventor
David H Gelfand
Frances C Lawyer
Susanne Stoffel
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE233816T1 publication Critical patent/ATE233816T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
AT91915802T 1990-08-13 1991-08-13 Gereinigtes thermostabiles nukleinsäure- polymeraseenzym aus thermotoga maritima ATE233816T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/567,244 US5374553A (en) 1986-08-22 1990-08-13 DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
PCT/US1991/005753 WO1992003556A1 (en) 1990-08-13 1991-08-13 PURIFIED THERMOSTABLE NUCLEIC ACID POLYMERASE ENZYME FROM $i(TERMOTOGA MARITIMA)

Publications (1)

Publication Number Publication Date
ATE233816T1 true ATE233816T1 (de) 2003-03-15

Family

ID=24266347

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91915802T ATE233816T1 (de) 1990-08-13 1991-08-13 Gereinigtes thermostabiles nukleinsäure- polymeraseenzym aus thermotoga maritima

Country Status (8)

Country Link
US (3) US5374553A (de)
EP (1) EP0544789B1 (de)
JP (3) JPH07108220B2 (de)
AT (1) ATE233816T1 (de)
AU (1) AU653747B2 (de)
DE (2) DE544789T1 (de)
ES (1) ES2193131T3 (de)
WO (1) WO1992003556A1 (de)

Families Citing this family (183)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455170A (en) * 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5756334A (en) * 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
JP2709311B2 (ja) * 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
ATE197718T1 (de) * 1990-09-28 2000-12-15 Hoffmann La Roche Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE69233253T2 (de) * 1991-06-03 2004-08-26 Takara Bio Inc., Otsu Verfahren zum Klonieren eines Pol-I-Typ DNS-Polymerase-Gens
US6410277B1 (en) * 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US7465539B1 (en) * 1993-02-19 2008-12-16 Takara Bio, Inc. DNA polymerases with enhanced length of primer extension
US7312052B1 (en) * 1993-12-08 2007-12-25 Stratagene California Polymerase compositions and uses thereof
US20050123926A1 (en) * 1994-01-13 2005-06-09 Enzo Diagnostics, Inc., In vitro process for producing multiple nucleic acid copies
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US6015668A (en) 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
AU4245096A (en) * 1994-11-04 1996-05-31 Molecular Biology Resources, Inc. Biologically active fragments of thermus flavus dna polymerase
US5935825A (en) * 1994-11-18 1999-08-10 Shimadzu Corporation Process and reagent for amplifying nucleic acid sequences
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US20030044956A1 (en) 1995-08-23 2003-03-06 Short Jay M. Enzymes having carboxymethyl cellulase activity and methods of use thereof
US5962258A (en) * 1995-08-23 1999-10-05 Diversa Corporation Carboxymethyl cellulase fromthermotoga maritima
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
WO1997009451A1 (en) * 1995-09-08 1997-03-13 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof
US20030027296A1 (en) * 1995-09-08 2003-02-06 Deb K. Chatterjee Cloned dna polymerases from thermotoga maritima and mutants thereof
AU1092297A (en) * 1995-12-07 1997-06-27 Novo Nordisk A/S Selective inactivation of enzyme activities
US5733732A (en) * 1996-01-03 1998-03-31 University Of Iowa Research Foundation Methods for detecting primary adhalinopathy
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
AU4065397A (en) 1996-08-14 1998-03-06 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
AU4174397A (en) * 1996-08-30 1998-03-19 Life Technologies, Inc. Methods for identification and isolation of specific nucleotide sequences in cdna and genomic dna
EP0835935A1 (de) * 1996-10-03 1998-04-15 Roche Diagnostics GmbH Thermostabile DNS Polymerase aus Anerocellum thermophilum
EP0834569A1 (de) * 1996-10-03 1998-04-08 Roche Diagnostics GmbH Thermostabile DNS Polymerase aus Carboxydothermus hydrogenoformans
EP0834570A1 (de) * 1996-10-03 1998-04-08 Roche Diagnostics GmbH Thermostabile DNS Polymerase aus Thermococcus gorgonarius
EP0834571A1 (de) * 1996-10-03 1998-04-08 Roche Diagnostics GmbH Thermostabile DNS Polymerase aus Thermococcus Gorgonarius
DE19647055A1 (de) 1996-11-14 1998-05-20 Boehringer Mannheim Gmbh Stabilisierte wässrige Nukleosidtriphosphat-Lösungen
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US20040142327A1 (en) * 1996-11-22 2004-07-22 Jhy-Jhu Lin Methods for identifying and isolating DNA polymorphisms or genetic markers
US5861295A (en) * 1997-01-02 1999-01-19 Life Technologies, Inc. Nucleic acid-free thermostable enzymes and methods of production thereof
US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
CA2283635A1 (en) 1997-03-21 1998-10-01 Stratagene Polymerase enhancing factor (pef) extracts, pef protein complexes, isolated pef protein, and methods for purifying and identifying
ES2355739T3 (es) 1997-04-03 2011-03-30 Life Technologies Corporation Composiciones y métodos para la reacción en cadena de la polimerasa de transcriptasa inversa (rt-pcr).
US7556958B1 (en) * 1997-04-08 2009-07-07 The Rockefeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
ATE445630T1 (de) * 1997-04-22 2009-10-15 Life Technologies Corp Verfahren zur herstellung von aslv reverser transkriptase zusammengestellt aus mehreren untereinheiten
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
DE19736062A1 (de) * 1997-08-20 1999-02-25 Boehringer Mannheim Gmbh Reduktion von Kreuzkontaminationen bei Nukleinsäureamplifikationen
EP2390258A1 (de) 1997-08-29 2011-11-30 Life Technologies Corporation Polymerasen mit hoher Wiedergabetreue und Verwendungen davon
EP0921196A1 (de) * 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modifizierte DNA Polymerase aus Carboxydothermus hydrogenoformans und deren Verwendung für gekoppelte reverse Transkription und Polymerase-kettenreaktion
EP0922765B1 (de) * 1997-12-02 2005-11-23 Roche Diagnostics GmbH Modifizierte DNA Polymerase aus Corboxydothermus hydrogenoformans und deren Verwendung für gekoppelte reverse Transkription und Polymerase-Kettenreaktion
WO1999042595A1 (en) * 1998-02-19 1999-08-26 Trevigen, Inc. Mismatch cleavage enzymes from extreme thermophiles and uses thereof
US5962296A (en) * 1998-02-24 1999-10-05 New England Biolabs, Inc. Method for cloning and producing Thermomicrobium roseum DNA polymerase I in E. coli
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
US7018827B1 (en) 1998-04-30 2006-03-28 The United States Of America As Represented By The Department Of Health And Human Services Nucleic acids of the M antigen gene of Histoplasma capsulatum, antigens, vaccines and antibodies, methods and kits for detecting histoplasmas
AU772847B2 (en) 1998-11-12 2004-05-06 Invitrogen Corporation Transfection reagents
US6485917B1 (en) * 1998-11-27 2002-11-26 Takara Shuzo Co., Ltd. Method for synthesizing cDNA
US7074556B2 (en) * 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
DE60020143T2 (de) * 1999-03-31 2006-02-23 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by the Secretary, Centers for Disease Control and Prevention, Office of Technology Transfer, Atlanta Typisierung von humanen enteroviren
WO2000068411A1 (en) * 1999-05-12 2000-11-16 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
US6869767B1 (en) 1999-06-11 2005-03-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Detection of Streptococcus pneumoniae and immunization against Streptococcus pneumoniae infection
WO2000079009A2 (en) 1999-06-22 2000-12-28 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
US6830902B1 (en) * 1999-07-02 2004-12-14 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
EP1212432A1 (de) * 1999-08-31 2002-06-12 Stratagene Verfahren zur reinigung von dna polymerasen
EP2210948A3 (de) 1999-12-10 2010-10-06 Life Technologies Corporation Verwendung von mehrfachen Rekombinationsstellen mit einzigartiger Spezifizität beim Rekombinationsklonen
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
CN1430670A (zh) 2000-05-26 2003-07-16 茵维特罗根公司 热稳定逆转录酶及其用途
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
NZ525336A (en) * 2000-10-20 2006-03-31 Expression Diagnostics Inc Leukocyte expression profiling
EP2105496B1 (de) 2000-12-08 2013-02-20 Life Technologies Corporation Verfahren und Zusammensetzungen zur Synthese von Nukleinsäuremolekülen unter Verwendung multipler Erkennungsstellen
US6509175B2 (en) * 2001-04-11 2003-01-21 Incyte Genomics, Inc. cDNA libraries and methods for their production
US6617136B2 (en) 2001-04-24 2003-09-09 3M Innovative Properties Company Biological sample processing methods and compositions that include surfactants
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
EP1275735A1 (de) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
EP1436385A4 (de) * 2001-09-14 2005-12-14 Invitrogen Corp Dna-polymerasen und mutanten davon
US20030165859A1 (en) * 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
US7148049B2 (en) * 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
AU2003240795A1 (en) * 2002-05-24 2003-12-12 Invitrogen Corporation Nested pcr employing degradable primers
US20040132133A1 (en) * 2002-07-08 2004-07-08 Invitrogen Corporation Methods and compositions for the production, identification and purification of fusion proteins
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
JP2006522582A (ja) * 2002-09-05 2006-10-05 インヴィトロジェン コーポレーション 核酸を合成するための組成物および方法
JP4634799B2 (ja) 2002-09-13 2011-02-16 ライフ テクノロジーズ コーポレーション 耐熱性逆転写酵素およびその使用法
US7662594B2 (en) * 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
EP1606419A1 (de) 2003-03-18 2005-12-21 Quantum Genetics Ireland Limited Systeme und methoden zur erhohung der milchproduktion in tieren
WO2004087868A2 (en) 2003-03-25 2004-10-14 Stratagene Dna polymerase fusions and uses thereof
DE10315640A1 (de) 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
US20040265870A1 (en) * 2003-04-09 2004-12-30 Invitrogen Corporation Methods of synthesizing and labeling nucleic acid molecules
WO2004094986A2 (en) 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US20060263813A1 (en) * 2005-05-11 2006-11-23 Expression Diagnostics, Inc. Methods of monitoring functional status of transplants using gene panels
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
WO2005030995A1 (en) * 2003-09-23 2005-04-07 University Of Missouri Methods of synthesizing polynucleotides using thermostable enzymes
EP2287341B1 (de) 2003-12-01 2013-02-13 Life Technologies Corporation Rekombinationsstellen enthaltende Nukleinsäuremoleküle und Verfahren zur Verwendung davon
BRPI0507929A (pt) 2004-02-19 2007-07-17 Univ Alberta polimorfismos de promotor de leptina e seus usos
SE0400441D0 (sv) * 2004-02-25 2004-02-25 Active Biotech Ab Novel Benzofurans and Indols
CA2560513A1 (en) 2004-04-08 2005-12-01 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
EP1754257B1 (de) 2004-06-07 2013-12-25 Fluidigm Corporation Optisches Linsensystem und Verfahren für mikrofluidische Einrichtungen
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
GB2416352B (en) * 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
CA2575675A1 (en) 2004-07-30 2006-03-09 Adeza Biomedical Corporation Oncofetal fibronectin as a marker for disease and other conditions and methods for detection of oncofetal fibronectin
WO2006029184A2 (en) 2004-09-08 2006-03-16 Expression Diagnostics, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US9505846B2 (en) * 2005-01-28 2016-11-29 Life Technologies Corporation Multi-component inhibitors of nucleic acid polymerases
US20060292578A1 (en) 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
US20070237716A1 (en) * 2005-11-30 2007-10-11 Mason Andrew L Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr) of human b-retrovirus
WO2008051290A2 (en) * 2006-04-07 2008-05-02 Xdx, Inc. Steroid responsive nucleic acid expression and prediction of disease activity
WO2007123386A1 (es) 2006-04-24 2007-11-01 Sigma Alimentos, S.A. De C.V. Método para la detección y cuantificación múltiple y simultánea de patógenos mediante la reacción en cadena de la polimerasa en tiempo real
US7993832B2 (en) 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
WO2008140484A2 (en) 2006-11-09 2008-11-20 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
EP1970440A1 (de) * 2007-03-06 2008-09-17 Qiagen GmbH Polymerasestabilisierung durch ionische Reinigungsmittel
JP5191041B2 (ja) * 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー 急速ワンステップｒｔ−ｐｃｒ
CN101688170A (zh) * 2007-04-24 2010-03-31 生物马特里卡公司 用于生命科学的样品存储
US8026091B2 (en) * 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods
EP2240576B1 (de) * 2008-01-11 2012-10-03 Genesys Ltd Chimäres cren7-protein
GB0803628D0 (en) * 2008-02-28 2008-04-02 Genesys Ltd Enzyme
GB0804721D0 (en) * 2008-03-14 2008-04-16 Genesys Ltd Enzyme
GB0804722D0 (en) * 2008-03-14 2008-04-16 Genesys Ltd Enzyme
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
WO2010050418A1 (ja) * 2008-10-28 2010-05-06 学校法人関西学院 変異型逆転写dnaポリメラーゼ
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
US8685648B2 (en) 2009-02-03 2014-04-01 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
EP2392680A1 (de) 2010-06-04 2011-12-07 Labor Diagnostik GmbH Leipzig Verfahren zum spezifischen Nachweis des klassischen Schweinepestvirus
WO2011163120A1 (en) 2010-06-21 2011-12-29 Life Technologies Corporation Compositions, methods and kits for nucleic acid synthesis and amplification
US9410194B2 (en) 2010-06-21 2016-08-09 Life Technologies Corporation Compositions, kits and methods for synthesis and/or detection of nucleic acids
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
EP2606149B1 (de) 2010-08-20 2017-08-02 Life Technologies Corporation Polymerasetest mit fret-substrat
US20120058481A1 (en) 2010-08-20 2012-03-08 Life Technologies Corporation Quantitative Real Time PCR Assay Using FRET Dual-Labeled Primers
CN103492587B (zh) 2010-12-13 2015-02-18 生命技术公司 利用通过多磷酸分解作用(app)反应的活化聚合核酸
US8715987B2 (en) 2011-05-02 2014-05-06 New England Biolabs, Inc. Solubilized phospholipids for stabilizing nucleic acid polymerases
US8663919B2 (en) 2011-05-18 2014-03-04 Life Technologies Corporation Chromosome conformation analysis
US8980333B2 (en) 2011-06-08 2015-03-17 Life Technologies Corporation Development of novel detergents for use in PCR systems
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
EP3293274B1 (de) 2011-08-10 2020-04-01 Life Technologies Corporation Polymerasezusammensetzungen
GB2518078B (en) 2012-06-18 2015-04-29 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
US9416405B2 (en) 2012-11-02 2016-08-16 Life Technologies Corporation Compositions, methods and kits for enhancing PCR specificity
WO2014100755A2 (en) 2012-12-20 2014-06-26 Biomatrica, Inc. Formulations and methods for stabilizing pcr reagents
CN105339505B (zh) 2013-03-12 2021-08-10 生命技术公司 基于通用报告体的基因分型方法和材料
WO2014150851A1 (en) 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9932623B2 (en) 2013-08-19 2018-04-03 Abbott Molecular Inc. Nucleotide analogs
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Ｐｃｒシステムにおいて使用するための新規化合物及びその用途
JP2017503521A (ja) 2014-01-22 2017-02-02 ライフ テクノロジーズ コーポレーション 高温核酸合成で使用するための新規な逆転写酵素
CN106572650B (zh) 2014-06-10 2021-08-31 生物马特里卡公司 在环境温度下稳定凝血细胞
EP3169310A1 (de) 2014-07-15 2017-05-24 Life Technologies Corporation Zusammensetzungen mit lipidaggregaten und verfahren zur effizienten bereitstellung von molekülen an zellen
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
ES2890776T3 (es) 2015-03-13 2022-01-24 Life Technologies Corp Métodos, composiciones y kits para la captura, la detección y la cuantificación del ARN pequeño
KR20180036712A (ko) 2015-08-24 2018-04-09 키아겐 게엠베하 Rna-서열결정 라이브러리를 생성하는 방법
WO2017070632A2 (en) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
AU2016368265B2 (en) 2015-12-08 2021-10-28 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
RU2757416C2 (ru) 2016-04-06 2021-10-15 Лайф Текнолоджис Корпорейшн Композиции, способы и наборы для синтеза и обнаружения нуклеиновых кислот
CN109477150B (zh) 2016-06-16 2023-03-31 生命技术公司 用于检测微生物的组合物、方法和试剂盒
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
EP3279337A1 (de) 2016-08-04 2018-02-07 Servizo Galego de Saúde (SERGAS) Verwendung kurzer sonden zwischen 8 und 9 nukleotiden in multiplextests
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US11613777B2 (en) 2016-08-26 2023-03-28 Life Technologies Corporation Nucleic acid extraction and amplification controls and methods of use thereof
GB2573062A (en) 2016-10-14 2019-10-23 Harvard College AAV delivery of nucleobase editors
EP3559231B1 (de) 2016-11-11 2022-08-17 Bio-Rad Laboratories, Inc. Verfahren zur verarbeitung von nukleinsäureproben
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
KR20190130613A (ko) 2017-03-23 2019-11-22 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
WO2019079347A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. USES OF BASIC EDITORS ADENOSINE
JP2021502825A (ja) 2017-11-13 2021-02-04 ライフ テクノロジーズ コーポレイション 尿路微生物検出のための組成物、方法、およびキット
MX2018008742A (es) 2018-07-16 2020-01-17 Sigma Alimentos Sa De Cv Metodo y kit de diagnostico para detectar multiple y simultaneamente una combinacion de bacterias gram positivas y/o bacterias gram negativas.
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
KR20210143230A (ko) 2019-03-19 2021-11-26 더 브로드 인스티튜트, 인코퍼레이티드 뉴클레오티드 서열을 편집하기 위한 방법 및 조성물
CN116096873A (zh) 2020-05-08 2023-05-09 布罗德研究所股份有限公司 同时编辑靶标双链核苷酸序列的两条链的方法和组合物
WO2022067130A2 (en) 2020-09-24 2022-03-31 The Broad Institute, Inc. Prime editing guide rnas, compositions thereof, and methods of using the same
EP4225914A1 (de) 2020-10-06 2023-08-16 Keygene N.V. Gezielte sequenzaddition
CA3203876A1 (en) 2021-01-11 2022-07-14 David R. Liu Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision
WO2023076898A1 (en) 2021-10-25 2023-05-04 The Broad Institute, Inc. Methods and compositions for editing a genome with prime editing and a recombinase

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
IL88923A (en) * 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
JP2531246B2 (ja) * 1988-08-26 1996-09-04 東洋紡績株式会社 耐熱性ｄｎａポリメラ―ゼおよびその製法
DK0506889T3 (da) * 1989-12-22 1997-09-22 Hoffmann La Roche Reverse transkriptaser ved høj temperatur

Also Published As

Publication number Publication date
WO1992003556A1 (en) 1992-03-05
JPH08298991A (ja) 1996-11-19
US5374553A (en) 1994-12-20
JP2584198B2 (ja) 1997-02-19
JPH07147990A (ja) 1995-06-13
EP0544789B1 (de) 2003-03-05
AU8501491A (en) 1992-03-17
DE544789T1 (de) 1993-12-16
US5420029A (en) 1995-05-30
DE69133204T2 (de) 2003-11-27
JPH07108220B2 (ja) 1995-11-22
JPH06500020A (ja) 1994-01-06
EP0544789A1 (de) 1993-06-09
US5624833A (en) 1997-04-29
DE69133204D1 (en) 2003-04-10
AU653747B2 (en) 1994-10-13
ES2193131T3 (es) 2003-11-01

Similar Documents

Publication Publication Date Title
ATE233816T1 (de) Gereinigtes thermostabiles nukleinsäure- polymeraseenzym aus thermotoga maritima
ATE135741T1 (de) Gereinigtes thermostabiles enzym
ES2152923T3 (es) Enzima polimerasa de acido nucleico termoestable purificada, de thermosipho africanus.
DE69133629D1 (de) nssystem zur Bestimmung von genetischen Krankheiten
EP0258017A3 (de) Gereinigtes thermostabiles Enzym und Verfahren zur Verstärkung, zum Nachweis und/oder zur Klonierung von Nukleinsäuresequenzen durch Verwendung dieses Enzyms
ATE530567T1 (de) Modifizierte, temperaturstabile dns-polymerasen für sequenzbestimmung
DE3486362D1 (de) Markiertes DNA.
ATE203056T1 (de) Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
ATE181106T1 (de) Mutationen in der 5'-3'-exonukleaseaktivität thermostabiler dna-polymerasen
DE69834056D1 (de) De novo oder "universaler" sequenzierungs-array
DE69419248D1 (de) Dns-polymerasen mit erhöhter temperaturstabilität und vergrössterter länge und effizient der primer-verlängerung
DK0624641T3 (da) Termostabil nukleinsyrepolymerase
EP0754765A3 (de) DNS Sequenzierung und DNS Kettenabbrecher
ES8705920A1 (es) Un metodo de preparacion de un oligonucleotido
NZ334653A (en) Thermostable nucleic acid polymerase with 3'-5' proofreading exonuclease ability from Thermococcus gorgonarius
CA2089495A1 (en) Purified thermostable nucleic acid polymerase enzyme from thermotoga maritima
Gelfand et al. Purified thermostable nucleic acid polymerase enzyme from thermotoga maritima
Gelfand et al. Mutated thermostable nucleic acid polymerase enzyme from thermotoga maritima

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0544789

Country of ref document: EP

EELA Cancelled due to lapse of time