MX2018008742A - Metodo y kit de diagnostico para detectar multiple y simultaneamente una combinacion de bacterias gram positivas y/o bacterias gram negativas. - Google Patents

Metodo y kit de diagnostico para detectar multiple y simultaneamente una combinacion de bacterias gram positivas y/o bacterias gram negativas.

Info

Publication number
MX2018008742A
MX2018008742A MX2018008742A MX2018008742A MX2018008742A MX 2018008742 A MX2018008742 A MX 2018008742A MX 2018008742 A MX2018008742 A MX 2018008742A MX 2018008742 A MX2018008742 A MX 2018008742A MX 2018008742 A MX2018008742 A MX 2018008742A
Authority
MX
Mexico
Prior art keywords
seq
gram
positive
combination
gram bacteria
Prior art date
Application number
MX2018008742A
Other languages
English (en)
Inventor
Magaly Rios Licea Merab
Jose De Haene Rosique Gregorio
Maria Guadalupe Rivas Estilla Ana
Original Assignee
Sigma Alimentos Sa De Cv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Alimentos Sa De Cv filed Critical Sigma Alimentos Sa De Cv
Priority to MX2018008742A priority Critical patent/MX2018008742A/es
Priority to EP19838597.3A priority patent/EP3825422A4/en
Priority to PCT/MX2019/000084 priority patent/WO2020017947A2/es
Publication of MX2018008742A publication Critical patent/MX2018008742A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método para detectar múltiple y simultáneamente una combinación de bacterias Gram positivas y/o bacterias Gram negativas en una muestra, el método comprende el proporcionar ADN extraído de la muestra; preparación de una mezcla de reacción que incluye: el ADN proporcionado; uno o más pares de iniciadores de oligonucleótido de secuencia seleccionada del grupo que consiste de SEQ ID NO: 1 y SEQ ID NO: 2, SEQ ID NO: 4 y SEQ ID NO: 5, SEQ ID NO: 7 y SEQ ID NO: 8, SEQ ID NO: 10 y SEQ ID NO: 11,SEQ ID NO: 13 y SEQ ID NO: 14, SEQ ID NO: 16 y SEQ ID NO: 17 y sus combinaciones; y uno o más sondas de oligonucleótido de secuencia seleccionada del grupo que consiste de SEQ ID NO: 3, SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 12, SEQ ID NO: 15, SEQ ID NO: 18 y sus combinaciones; amplificación, mediante PCR en tiempo real, del ADN proporcionado en la mezcla de reacción; y determinación simultánea de presencia o ausencia de las bacterias Gram positivas y/o bacterias Gram negativas en la muestra. El método permite identificar cualquier combinación de tres especies de bacterias Gram positivas (Listeria ssp., Listeria monocytogenes y Staphylococcus aureus) y tres especies de bacterias Gram negativas (Escherichia coli spp., Escherichia coli O157:H7 y Salmonella spp.).
MX2018008742A 2018-07-16 2018-07-16 Metodo y kit de diagnostico para detectar multiple y simultaneamente una combinacion de bacterias gram positivas y/o bacterias gram negativas. MX2018008742A (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
MX2018008742A MX2018008742A (es) 2018-07-16 2018-07-16 Metodo y kit de diagnostico para detectar multiple y simultaneamente una combinacion de bacterias gram positivas y/o bacterias gram negativas.
EP19838597.3A EP3825422A4 (en) 2018-07-16 2019-07-11 DIAGNOSTIC KIT AND METHOD FOR SIMULTANEOUSLY DETECTING A COMBINATION OF MULTIPLE GRAM POSITIVE BACTERIA AND/OR GRAM NEGATIVE BACTERIA
PCT/MX2019/000084 WO2020017947A2 (es) 2018-07-16 2019-07-11 Método y kit de diagnóstico para detectar múltiple y simultáneamente una combinación de bacterias gram positivas y/o bacterias gram negativas

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX2018008742A MX2018008742A (es) 2018-07-16 2018-07-16 Metodo y kit de diagnostico para detectar multiple y simultaneamente una combinacion de bacterias gram positivas y/o bacterias gram negativas.

Publications (1)

Publication Number Publication Date
MX2018008742A true MX2018008742A (es) 2020-01-17

Family

ID=69165225

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018008742A MX2018008742A (es) 2018-07-16 2018-07-16 Metodo y kit de diagnostico para detectar multiple y simultaneamente una combinacion de bacterias gram positivas y/o bacterias gram negativas.

Country Status (3)

Country Link
EP (1) EP3825422A4 (es)
MX (1) MX2018008742A (es)
WO (1) WO2020017947A2 (es)

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5008182A (en) 1986-01-10 1991-04-16 Cetus Corporation Detection of AIDS associated virus by polymerase chain reaction
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5176995A (en) 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US5038852A (en) 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US5386022A (en) 1985-03-28 1995-01-31 Hoffman-La Roche Inc. Primes and probes for the amplification and detection of aids associated nucleic acids
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5310893A (en) 1986-03-31 1994-05-10 Hoffmann-La Roche Inc. Method for HLA DP typing
US5405774A (en) 1986-08-22 1995-04-11 Hoffmann-La Roche Inc. DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5352600A (en) 1986-08-22 1994-10-04 Hoffmann-La Roche Inc. Purified thermostable enzyme
US5407800A (en) 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
EP0540693B1 (en) 1990-07-24 1999-01-20 F. Hoffmann-La Roche Ag THE REDUCTION OF NON-SPECIFIC AMPLIFICATION DURING $i(IN VITRO) NUCLEIC ACID AMPLIFICATION USING MODIFIED NUCLEIC ACID BASES
CA2231184A1 (en) 1995-09-20 1997-03-27 George Tice Jr. Control for pcr
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
GB9904804D0 (en) 1999-03-02 1999-04-28 King S College London Identification of bacteria
EP1495135A4 (en) 2001-08-08 2005-05-04 Qualicon Inc FAST AND SPECIFIC CAMPYLOBACTER PROOF
BRPI0710889A2 (pt) * 2006-04-24 2011-08-16 Sigma Alimentos Sa De Cv método para a detecção e quantificação múltipla e simultánea de patogênicos mediante a reação em cadeia da polimerasa em tempo real

Also Published As

Publication number Publication date
WO2020017947A2 (es) 2020-01-23
EP3825422A2 (en) 2021-05-26
EP3825422A4 (en) 2022-04-20
WO2020017947A3 (es) 2020-03-19

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