CA2089495A1 - Purified thermostable nucleic acid polymerase enzyme from thermotoga maritima - Google Patents
Purified thermostable nucleic acid polymerase enzyme from thermotoga maritimaInfo
- Publication number
- CA2089495A1 CA2089495A1 CA002089495A CA2089495A CA2089495A1 CA 2089495 A1 CA2089495 A1 CA 2089495A1 CA 002089495 A CA002089495 A CA 002089495A CA 2089495 A CA2089495 A CA 2089495A CA 2089495 A1 CA2089495 A1 CA 2089495A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- thermotoga maritima
- enzyme
- acid polymerase
- polymerase enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 241000204666 Thermotoga maritima Species 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 102000004594 DNA Polymerase I Human genes 0.000 abstract 1
- 108010017826 DNA Polymerase I Proteins 0.000 abstract 1
- 241000186394 Eubacterium Species 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 238000001502 gel electrophoresis Methods 0.000 abstract 1
- 239000002777 nucleoside Substances 0.000 abstract 1
- -1 nucleoside triphosphates Chemical class 0.000 abstract 1
- 239000001226 triphosphate Substances 0.000 abstract 1
- 235000011178 triphosphate Nutrition 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima, The enzyme has a molecular weight as determined by gel electrophoresis of about 97 kilodaltons and DNA
polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US567,244 | 1990-08-13 | ||
| US07/567,244 US5374553A (en) | 1986-08-22 | 1990-08-13 | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| PCT/US1991/005753 WO1992003556A1 (en) | 1990-08-13 | 1991-08-13 | PURIFIED THERMOSTABLE NUCLEIC ACID POLYMERASE ENZYME FROM $i(TERMOTOGA MARITIMA) |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2089495A1 true CA2089495A1 (en) | 1992-02-14 |
| CA2089495C CA2089495C (en) | 2007-04-03 |
Family
ID=37951652
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002089495A Expired - Lifetime CA2089495C (en) | 1990-08-13 | 1991-08-13 | Purified thermostable nucleic acid polymerase enzyme from thermotoga maritima |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2089495C (en) |
-
1991
- 1991-08-13 CA CA002089495A patent/CA2089495C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2089495C (en) | 2007-04-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |