CA2089495A1 - Purified thermostable nucleic acid polymerase enzyme from thermotoga maritima - Google Patents

Purified thermostable nucleic acid polymerase enzyme from thermotoga maritima

Info

Publication number
CA2089495A1
CA2089495A1 CA002089495A CA2089495A CA2089495A1 CA 2089495 A1 CA2089495 A1 CA 2089495A1 CA 002089495 A CA002089495 A CA 002089495A CA 2089495 A CA2089495 A CA 2089495A CA 2089495 A1 CA2089495 A1 CA 2089495A1
Authority
CA
Canada
Prior art keywords
nucleic acid
thermotoga maritima
enzyme
acid polymerase
polymerase enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002089495A
Other languages
French (fr)
Other versions
CA2089495C (en
Inventor
David H. Gelfand
Frances C. Lawyer
Susanne Stoffel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
David H. Gelfand
Frances C. Lawyer
Susanne Stoffel
F. Hoffmann-La Roche Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/567,244 external-priority patent/US5374553A/en
Application filed by David H. Gelfand, Frances C. Lawyer, Susanne Stoffel, F. Hoffmann-La Roche Ag filed Critical David H. Gelfand
Publication of CA2089495A1 publication Critical patent/CA2089495A1/en
Application granted granted Critical
Publication of CA2089495C publication Critical patent/CA2089495C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima, The enzyme has a molecular weight as determined by gel electrophoresis of about 97 kilodaltons and DNA
polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
CA002089495A 1990-08-13 1991-08-13 Purified thermostable nucleic acid polymerase enzyme from thermotoga maritima Expired - Lifetime CA2089495C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/567,244 US5374553A (en) 1986-08-22 1990-08-13 DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US567,244 1990-08-13
PCT/US1991/005753 WO1992003556A1 (en) 1990-08-13 1991-08-13 PURIFIED THERMOSTABLE NUCLEIC ACID POLYMERASE ENZYME FROM $i(TERMOTOGA MARITIMA)

Publications (2)

Publication Number Publication Date
CA2089495A1 true CA2089495A1 (en) 1992-02-14
CA2089495C CA2089495C (en) 2007-04-03

Family

ID=37951652

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002089495A Expired - Lifetime CA2089495C (en) 1990-08-13 1991-08-13 Purified thermostable nucleic acid polymerase enzyme from thermotoga maritima

Country Status (1)

Country Link
CA (1) CA2089495C (en)

Also Published As

Publication number Publication date
CA2089495C (en) 2007-04-03

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Legal Events

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